BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0010.p (293 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate A... 510 e-146 gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporte... 146 2e-36 gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate A... 132 5e-32 gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate A... 107 1e-24 gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporte... 107 2e-24 >gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 510 bits (1314), Expect = e-146, Method: Compositional matrix adjust. Identities = 261/274 (95%), Positives = 264/274 (96%) Query: 1 MQRRDFLRLAPAALGATVLGSAPLLARAAQPPKTIRLDYAYYSPPSLVLRKFGWLEEDLK 60 M RRDFLRLAPAALG T LG PLL+ AAQPPK IRLDYAYYSPPSLVLRKFGWLEEDLK Sbjct: 1 MHRRDFLRLAPAALGVTALGGIPLLSSAAQPPKVIRLDYAYYSPPSLVLRKFGWLEEDLK 60 Query: 61 AQGTEVKWVLSAGSNRALEYLNSDSVDFGSTAGLAALLARANGNPVKAVYIYSRPEWTAL 120 AQGTEVKWVLS GSNRALEYLNSDSVDFGSTAGLAALLARANGNPVKAVYIYSRPEWTAL Sbjct: 61 AQGTEVKWVLSQGSNRALEYLNSDSVDFGSTAGLAALLARANGNPVKAVYIYSRPEWTAL 120 Query: 121 AVGKESTVKSITELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQHADGRAALE 180 AVGKES VKSI ELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQHADGRAALE Sbjct: 121 AVGKESPVKSINELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQHADGRAALE 180 Query: 181 QGRVAAWAGLDPHLAASELEAGSRLIYRNVNFNTYGFLNVSDTFAARHPDQVKRVIAAYE 240 QGRVAAWAGLDPHLAASELEAGSRLIYRNVNFNTYGFLNVSD+FAARHPDQVKRVIAAYE Sbjct: 181 QGRVAAWAGLDPHLAASELEAGSRLIYRNVNFNTYGFLNVSDSFAARHPDQVKRVIAAYE 240 Query: 241 RARTWIIAHPDETVALLSEESKLSAPVARLQLKR 274 RAR+WIIAHPDETVALLSEESKLSAPVARLQLKR Sbjct: 241 RARSWIIAHPDETVALLSEESKLSAPVARLQLKR 274 >gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 327 Score = 146 bits (368), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 108/306 (35%), Positives = 157/306 (51%), Gaps = 18/306 (5%) Query: 2 QRRDFLRLAPAALGA--TVLGSAPLLARAAQPPKTIRLDYAYYSPPSLVLRKFGWLEEDL 59 +RR RL+ ALGA LG++P+ AQ K +R+ Y + ++L+ G LE+ L Sbjct: 13 RRRAIQRLSAIALGALTIALGASPV---QAQETKLLRIGYQKAANTLVLLKAHGTLEKRL 69 Query: 60 KAQGTEVKWVLSAGSNRALEYLNSDSVDFGSTAGLAALLARANG-NPVKAVYIYSRPEWT 118 + G EVKW A + LE LN SVDFG + A+A G + V Y P+ Sbjct: 70 QPLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFVYTAYEIPTPDAE 129 Query: 119 ALAVGKESTVKSITELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQHADGRAA 178 L V K S +KS+ ELKGKKIA KG+D + ++ L + GL+ SD++ V+L AD RAA Sbjct: 130 GLLVQKNSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQPVYLAPADARAA 189 Query: 179 LEQGRVAAWAGLDPHLAASELEAGSRLIYRNVN-FNTYGFLNVSDTFAARHPDQVKRVIA 237 E+G V AWA DP AA+E + G+R + V N + F + FAA++P K ++ Sbjct: 190 FERGAVDAWAIWDPFQAAAEKQVGARRLATGVGAVNHHQFFLSARPFAAKNPQVEKILLE 249 Query: 238 AYERARTWIIAHPDETVALLSEESKLSAPVARLQLKR-----------XLSEESKLSAPV 286 R W+ H + A L+ L A + LKR L+E+ K++ Sbjct: 250 EISREGEWVRTHTAQAAAQLAPIQGLDADIIETGLKRYAHVYKPVDAQVLAEQQKIADAF 309 Query: 287 ARLQLI 292 L+LI Sbjct: 310 YELKLI 315 >gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 323 Score = 132 bits (331), Expect = 5e-32, Method: Compositional matrix adjust. Identities = 96/278 (34%), Positives = 142/278 (51%), Gaps = 9/278 (3%) Query: 2 QRRDFLRLAPAALGATVLGSAPLLARAAQPPKTIRLDYAYYSPPSLVLRKFGWLEEDLKA 61 Q R RL L A L S L RAA K +R+ Y ++ + +L+ G LE+ L+ Sbjct: 5 QPRHKRRLLLQGLAAGALASGTPLVRAAAD-KVLRIGYQKFNTLN-ILKGSGKLEQALQP 62 Query: 62 QGTEVKWVLSAGSNRALEYLNSDSVDFGSTAGLAALLARANGNPVKAVYIYSR---PEWT 118 QG VKW A + E LN++++D G A ++ A A+G V AVY+ + P+ Sbjct: 63 QGWSVKWFEFAAGPQLTEALNANAIDLGHAADTPSVFANASG--VNAVYLGAEQPYPKGI 120 Query: 119 ALAVGKESTVKSITELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQH-ADGRA 177 + V +ES ++S+ +LKGKK+A +G + LL+AL E GL D+E V++ + AD RA Sbjct: 121 GIFVAQESPIRSVADLKGKKVATGRGWNVQFLLLKALEEVGLSYRDIEPVYVTNAADVRA 180 Query: 178 ALEQGRVAAWAGLDPHLAASELEAGSRLIYRNVNF-NTYGFLNVSDTFAARHPDQVKRVI 236 A + G V A DP LA EL R++ N F +F RH D V+ V Sbjct: 181 AFQSGNVDAATLWDPFLAGQELATRPRILRDGTGLSNNRTFHLAVPSFVDRHKDIVRTVF 240 Query: 237 AAYERARTWIIAHPDETVALLSEESKLSAPVARLQLKR 274 A ++ W +HP ET LL+ + + V RL +R Sbjct: 241 AELKKTNQWTQSHPQETAELLAPQLGVDPKVLRLATER 278 >gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 107 bits (267), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 84/249 (33%), Positives = 120/249 (48%), Gaps = 6/249 (2%) Query: 16 ATVLGSAPLLARA---AQPPKTIRLDYAYYSPPSLVLRKFGWLEEDLKAQGTEVKWVLSA 72 AT LG LA A AQ K IR+ Y S +L+ G LE+ L G ++ W A Sbjct: 17 ATALGLGSALAPASVFAQGNKAIRIGYQKSSTLLTILKANGTLEKLLAPTGAKISWHEFA 76 Query: 73 GSNRALEYLNSDSVDFGS-TAGLAALLARANGNPVKAVYIYS-RPEWTALAVGKESTVKS 130 LE LN VD + A + A+A G + V S P A+ V +S ++ Sbjct: 77 SGLPLLEALNVGGVDLSADVADTVPVFAQAAGAKLAYVAQESPSPAAQAIVVRADSVLRK 136 Query: 131 ITELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQHADGRAALEQGRVAAWAGL 190 ++LKGKKIA TK + L+ L + GLK SDV+ +L ADGRAA E+G V AW Sbjct: 137 ASDLKGKKIAVTKAAGVHYLLIATLEKAGLKFSDVDAAYLSPADGRAAFERGSVDAWVTW 196 Query: 191 DPHLAASELEAGSRLIYRNVNFNTYG-FLNVSDTFAARHPDQVKRVIAAYERARTWIIAH 249 DP LA + ++ R++ Y + S +FA P+ ++ V ++ W+ H Sbjct: 197 DPFLAGVQRQSQVRILSDGRGVADYQRYYLASASFAQARPEVLRVVFDELQKTGHWVKQH 256 Query: 250 PDETVALLS 258 P E A L+ Sbjct: 257 PKEAAAFLA 265 >gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 107 bits (266), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 74/216 (34%), Positives = 114/216 (52%), Gaps = 8/216 (3%) Query: 48 VLRKFGWLEEDLKAQGTEVKWVLSAGSNRALEYLNSDSVDFGSTAGLAALLARANGNPVK 107 ++R G LE+ L G VKWV + LE L + S+DFG + A+A G P+ Sbjct: 64 LIRAHGVLEQRLAPLGATVKWVEFSAGPVQLEALGAGSIDFGDVGEAPPIFAQAAGTPL- 122 Query: 108 AVYI---YSRPEWTALAVGKESTVKSITELKGKKIAATKGTDPYLFLLRALHENGLKKSD 164 VY+ RP A+ V +S ++ +LKGK+IA KG++ + FL++ L ++GLK D Sbjct: 123 -VYVGATVPRPYVEAVLVKDDSELQRPQQLKGKRIALNKGSNVHYFLVKLLEKHGLKYGD 181 Query: 165 VEIVHLQHADGRAALEQGRVAAWAGLDPHLAASELEAGSRLI--YRNVNFNTYGFLNVSD 222 V V L AD RAA E+G V AWA +P+LAA++ +R++ V N + + D Sbjct: 182 VTPVWLAPADARAAFERGAVDAWAIWEPYLAAAQQGLKARILADADGVVKNRAYYFSTRD 241 Query: 223 TFAARHPDQVKRVIAAYERARTWIIAHPDETVALLS 258 +A H D ++ + + W A PDE + L+ Sbjct: 242 -YARDHADLLRIALEELGKIDAWAQARPDEYASELA 276 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.131 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 879,590 Number of extensions: 34395 Number of successful extensions: 172 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 164 Number of HSP's successfully gapped: 7 Length of query: 293 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 209 Effective length of database: 1,222,130 Effective search space: 255425170 Effective search space used: 255425170 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)