BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0004.p (527 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314061|ref|YP_003778153.1| periplasmic glucan biosynthesis... 1028 0.0 gi|300312745|ref|YP_003776837.1| periplasmic glucans biosynthesi... 464 e-132 gi|300313221|ref|YP_003777313.1| periplasmic glucans biosynthesi... 302 3e-83 >gi|300314061|ref|YP_003778153.1| periplasmic glucan biosynthesis protein [Herbaspirillum seropedicae SmR1] Length = 517 Score = 1028 bits (2657), Expect = 0.0, Method: Compositional matrix adjust. Identities = 496/513 (96%), Positives = 505/513 (98%) Query: 15 VLSASLLAASVQAQSAQGFDLNQAVARAKELAAKPYQKPVSNLSPTFAAMQFADYMKIQP 74 LSA LLAAS QAQ++QGFDLNQAVARAK+LAAKPYQKPVSNLSPTFAAMQFADYMKIQP Sbjct: 5 TLSACLLAASAQAQTSQGFDLNQAVARAKDLAAKPYQKPVSNLSPTFAAMQFADYMKIQP 64 Query: 75 KSEKFEWRDQKTPFKLAFYHQGMQFNAPVAIHEIIDGRVQDVGYSTDRFNFGDLALAKDS 134 KSEKFEWRDQKTPFKLAFYHQGMQFNAPVAIHEIIDGRVQDVGYSTDRFNFGDL LAKDS Sbjct: 65 KSEKFEWRDQKTPFKLAFYHQGMQFNAPVAIHEIIDGRVQDVGYSTDRFNFGDLNLAKDS 124 Query: 135 TAHLGYAGFRVVYPLNRDDKEDEVMSVLGASYFRVIGKGQVYGLSARGLAIDTAMMSGEE 194 TAHLGYAGFRVVYP+NRDDKEDEVMSVLGASYFRVIGKGQVYGLSARGLAIDTAMMSGEE Sbjct: 125 TAHLGYAGFRVVYPINRDDKEDEVMSVLGASYFRVIGKGQVYGLSARGLAIDTAMMSGEE 184 Query: 195 FPHFTEFWIQRPAPGEKQLTFYALLDSPRATGAYKFTLTPGKDALLDVQARVFLRAEVGR 254 FPHFTEFWIQRPAPGEKQLTFYALLDSPRATGAYKFTLTPGKDALLDVQARVFLR EVGR Sbjct: 185 FPHFTEFWIQRPAPGEKQLTFYALLDSPRATGAYKFTLTPGKDALLDVQARVFLRGEVGR 244 Query: 255 LGIAPLTSMFLFGPNQQSSRRNFRPAIHDSNGLAIHTGNGEWLWRPLNDPQNVEVSSFEV 314 LGIAPLTSMFLFGPNQQSSRRNFRPAIHDSNGLAIHTGNGEWLWRPLNDPQNVEVSSFEV Sbjct: 245 LGIAPLTSMFLFGPNQQSSRRNFRPAIHDSNGLAIHTGNGEWLWRPLNDPQNVEVSSFEV 304 Query: 315 SNPRGFGLLQRGREFSSYEDLKDRYDLRPSAWIEPKGDWGKGAVQLVEIPTADETNDNIV 374 SNPRGFGLLQRGREFS+YEDLKDRYDLRPSAWIEPKGDWGKGAVQLVEIPTADETNDNIV Sbjct: 305 SNPRGFGLLQRGREFSAYEDLKDRYDLRPSAWIEPKGDWGKGAVQLVEIPTADETNDNIV 364 Query: 375 AYWVPAQQPARGTPLAYDYRIHWTMDEPAILNNEVGWVKQTFHTDGELTQANLIRAFDGT 434 AYW+PAQ PARGTPLAYDYRIHWTMDEPAILNNEVGWVKQTFHTDGELTQANLIRAFDGT Sbjct: 365 AYWLPAQLPARGTPLAYDYRIHWTMDEPAILNNEVGWVKQTFHTDGELTQANLIRAFDGT 424 Query: 435 TALLVDFTGGPLGSLPAGTAVQPQVNVSNNGELIDAQLQPNPAINGWRLTLRVKIRNVAQ 494 TALLVDFTGGPLG+LPAGT VQPQV+VSNNGELID QLQPNPAINGWRLTLRVKIRNVAQ Sbjct: 425 TALLVDFTGGPLGALPAGTPVQPQVSVSNNGELIDVQLQPNPAINGWRLTLRVKIRNVAQ 484 Query: 495 AVELRAALTANGKPLTETWSYQLPPRSDVQRTF 527 AVELRAAL+ANGKPLTETWSYQLPPRSDVQRTF Sbjct: 485 AVELRAALSANGKPLTETWSYQLPPRSDVQRTF 517 >gi|300312745|ref|YP_003776837.1| periplasmic glucans biosynthesis G protein [Herbaspirillum seropedicae SmR1] Length = 541 Score = 464 bits (1194), Expect = e-132, Method: Compositional matrix adjust. Identities = 235/490 (47%), Positives = 312/490 (63%), Gaps = 7/490 (1%) Query: 33 FDLNQAVARAKELAAKPYQKPVSNLSPTFAAMQFADYMKIQPKSEKFEWRDQKTPFKLAF 92 FD N ARAK LA K Y+KP L + + + +I + +K WR QK PF+L+F Sbjct: 55 FDFNTVAARAKALAGKSYKKPADELPKSLKDLSYEQASQIYFRDDKSYWRAQKLPFELSF 114 Query: 93 YHQGMQFNAPVAIHEIIDGRVQDVGYSTDRFNF-GDLALAKDSTAHLGYAGFRVVYPLNR 151 H G + P+ ++E++ V+++ ++ F++ ++ S +LG+AGFRV +P+N Sbjct: 115 IHLGASYTEPIKVNEVVGDAVREIHFNPALFDYSATRSVDPRSLRNLGFAGFRVRFPVNS 174 Query: 152 DDKEDEVMSVLGASYFRVIGKGQVYGLSARGLAIDTAMMSGEEFPHFTEFWIQRPAPGEK 211 +D+V++ GASYFR +GKGQ YGLSARGLAIDTA+ SGEEFP FTEFW++RP+ K Sbjct: 175 AKVKDDVLTFHGASYFRALGKGQSYGLSARGLAIDTALYSGEEFPRFTEFWLERPSAESK 234 Query: 212 QLTFYALLDSPRATGAYKFTLTPGKDALLDVQARVFLRAEVGRLGIAPLTSMFLFGPNQQ 271 +L YALLDSPRATGAY+F L PG D ++DV+A+++LR+ V +LGIAPLTSMFLFG NQ Sbjct: 235 ELVIYALLDSPRATGAYRFVLKPGADTVMDVKAQLYLRSNVTKLGIAPLTSMFLFGENQP 294 Query: 272 SSRRNFRPAIHDSNGLAIHTGNGEWLWRPLNDPQNVEVSSFEVSNPRGFGLLQRGREFSS 331 +FRP +HDS+GLA+ +G GEWLWRPL +P+ + V+SF NP GFGL+QR R FS Sbjct: 295 GPADDFRPEVHDSDGLAMQSGTGEWLWRPLVNPKRLLVTSFSFDNPLGFGLMQRDRSFSH 354 Query: 332 YEDLKDRYDLRPSAWIEPKGDWGKGAVQLVEIPTADETNDNIVAYWVPAQQPARGTPLAY 391 YEDL Y RPSAW+EPKG WG G V+LV+IPT DETNDNIVAYW+P P G P Sbjct: 355 YEDLDYNYQARPSAWVEPKGKWGSGRVELVQIPTPDETNDNIVAYWIPNTLPKPGQPFNV 414 Query: 392 DYRIHWTMDEPAILNNEVGWVKQTFHTDGELTQANLIRAFDGTTALLVDFTGGPLGSLPA 451 +YR+ W D + +V QT G + D L +DF GG L LPA Sbjct: 415 EYRLSWQKDNEK--RPPLAYVTQTLFGHGYRPKQE--GKTDDVQQLSIDFDGGNLKKLPA 470 Query: 452 GTAVQPQVNVSNNGELIDAQLQPNPAINGWRLTLRVKIRNVAQAVELRAAL-TAN-GKPL 509 V+ V NG+L + N A GWR+ ++++ + VELR L AN G L Sbjct: 471 NARVEGVVEADANGKLASVTTRKNEATGGWRVEVKLRRTEENKPVELRGFLRNANGGTTL 530 Query: 510 TETWSYQLPP 519 +ETWSY LPP Sbjct: 531 SETWSYILPP 540 >gi|300313221|ref|YP_003777313.1| periplasmic glucans biosynthesis protein [Herbaspirillum seropedicae SmR1] Length = 544 Score = 302 bits (774), Expect = 3e-83, Method: Compositional matrix adjust. Identities = 189/525 (36%), Positives = 277/525 (52%), Gaps = 37/525 (7%) Query: 17 SASLLAASVQAQSAQGFDLNQAVARAKELAAKPYQKPVSNLSPTFAAMQFADYMKIQPKS 76 S +L A ++ + Q F Q + RA+++A+ PYQ + + + + + KI+ K Sbjct: 28 SGALAAQGIETSAPQPFSFAQLIERARQMASTPYQPAAAAPAAILDRIDYDAHGKIRFKP 87 Query: 77 EKFEWRDQKTPFKLAFYHQGMQFNAPVAIHEIIDG--------RVQDVGYSTDRFNFGDL 128 ++ + PF L F+H G F PV + +++G + +++ Y D F+ Sbjct: 88 DRAAFATGPGPFPLTFFHLGKFFRVPVRMF-LLEGAGDATAPTQARELRYRDDYFD---- 142 Query: 129 ALAKDSTAHL-----GYAGFRVVYPL-----NRDDKEDEVMSVLGASYFRVIGKGQVYGL 178 + DS AH G+AGFR +D ++++ ++ LGASYFR IG YGL Sbjct: 143 -MPADSPAHALPPGSGFAGFRFQESRLGDQQVKDWRKNDWVAFLGASYFRAIGDLYQYGL 201 Query: 179 SARGLAIDTAMM-SGEEFPHFTEFWIQRPAPGEKQLTFYALLDSPRATGAYKFTLTPGKD 237 SARG+AID A+ EEFP FT+F+ + APG + YALLD P TGAY+F + G+ Sbjct: 202 SARGVAIDVAVAGKSEEFPDFTQFFFEPGAPGSDTVVVYALLDGPSITGAYRFAMQRGQG 261 Query: 238 ALLDVQARVFLRAEVGRLGIAPLTSMFLFGPNQQSSRRNFRPAIHDSNGLAIHTGNGEWL 297 +++ ++FLR +V RLG+AP TSM+ + + + ++RP +HDS+GLAI G GE L Sbjct: 262 VTMEIDKQLFLRRDVERLGLAPATSMYWYSETVKGTGVDWRPEVHDSDGLAIWNGAGEHL 321 Query: 298 WRPLNDPQNVEVSSFEVSNPRGFGLLQRGREFSSYEDLKDRYDLRPSAWIEPKGDWGKGA 357 WRPLN+P SSF PRGFGL+QR R+F Y D Y+ RPS W+EP GDWG+G Sbjct: 322 WRPLNNPPRTIASSFADRQPRGFGLMQRDRDFDHYLD-GVHYERRPSLWVEPLGDWGEGQ 380 Query: 358 VQLVEIPTADETNDNIVAYWVPAQQPARGTPLAYDYRIHWTMDEPAILNNEVGWVKQTFH 417 VQL+E+ T DE +DNIVA WVP G YR+HW + P + V Sbjct: 381 VQLIELRTDDEIHDNIVAMWVPKAAARAGDRYQLRYRLHWQSETP-FPSALARCVATRLG 439 Query: 418 TDGELTQAN--LIRAFDGTTALLVDFTGGPLGSLPAGTAVQPQVNVSNNG-ELIDAQLQP 474 G+ Q +R F +V+F G PL +LP G + ++ S + + P Sbjct: 440 RGGQPGQPRPPQVRKF------MVEFKGPPLQALPFGVKPEAVISASRGSISYVYTEALP 493 Query: 475 NPAINGWRLTLRVKIRNVAQAVELRAALTANGKPLTETWSYQLPP 519 N WR + + VELR L G+ L+ETW +Q P Sbjct: 494 NGVPGHWRAQFDLTAEG-EEPVELRLFLRHQGQALSETWLFQYHP 537 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,745,096 Number of extensions: 77937 Number of successful extensions: 186 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 178 Number of HSP's successfully gapped: 3 Length of query: 527 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 437 Effective length of database: 1,193,720 Effective search space: 521655640 Effective search space used: 521655640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)