BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0238.p (597 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313000|ref|YP_003777092.1| AMP-binding enzyme family prote... 939 0.0 gi|300311658|ref|YP_003775750.1| non-ribosomal peptide synthetas... 56 7e-09 >gi|300313000|ref|YP_003777092.1| AMP-binding enzyme family protein [Herbaspirillum seropedicae SmR1] Length = 586 Score = 939 bits (2427), Expect = 0.0, Method: Compositional matrix adjust. Identities = 472/586 (80%), Positives = 511/586 (87%), Gaps = 1/586 (0%) Query: 13 MNTTAQLDLLQLPFQAFDGEHVIGWRDGTALTHAAWRAEVLRWRQLITATPGERIALFHH 72 M +LDLLQLPF AF G VIGWRDG A+THA+WRAE+ RW+ L+ TPG+R+ALFHH Sbjct: 1 MTAAPRLDLLQLPFLAFAGADVIGWRDGVAVTHASWRAELRRWQHLVQTTPGQRMALFHH 60 Query: 73 DSLHFAAALFGIWLAGKTAYLPSDTLPETCRQLRTEVDAFAGDFGAHCPALWPAADAVPD 132 D+L FAAALF IWLAGKTAYLPSD LP+TCRQL+ EVDAF G+FGAHCPAL PA DA Sbjct: 61 DTLQFAAALFAIWLAGKTAYLPSDALPDTCRQLQGEVDAFLGEFGAHCPALHPAGDAAEG 120 Query: 133 DALAPWPALRADFTGLVVYTSGSTGQAQAIPKQLGQMANEVATLEALFGSIAGQADVVAT 192 D WP LRADF GLVVYTSGSTG AQAIPKQLGQMANEVATLEALFG+I G+A+V+AT Sbjct: 121 DTPDAWPTLRADFGGLVVYTSGSTGLAQAIPKQLGQMANEVATLEALFGAIVGEAEVIAT 180 Query: 193 VSHQHIYGLLFKVLWALCAGRPIHAGSAFFPEDLQRMTGPRPWLLLSSPAHLTRLPEQVT 252 VSHQHIYGLLFKVLWALCAGRP+HAGSAFFPEDLQRM G RPW+LLSSPAHL RLPEQ Sbjct: 181 VSHQHIYGLLFKVLWALCAGRPVHAGSAFFPEDLQRMAGARPWILLSSPAHLKRLPEQGA 240 Query: 253 QPDPRQLRAIFSSGGPLPAQAARDAARLLGHRPLEIYGSSETGGIAWRQPESDLLQRREH 312 QPD RQLRAIFSSGGPLPAQAA D+ARLLG RPLEIYGSSETGGIAWRQP++D LQR+EH Sbjct: 241 QPDARQLRAIFSSGGPLPAQAAADSARLLGQRPLEIYGSSETGGIAWRQPQTDPLQRQEH 300 Query: 313 WQAMPGVAVRIGAEGALEVRSPHLPDDQWFSMADRAVLVDDLPAAFELRGRSDRIVKLEE 372 WQAMPGV VR+GAEG LEVRSPHLPD WF MADRA LV P FELRGRSDRIVKLEE Sbjct: 301 WQAMPGVEVRLGAEGELEVRSPHLPDSAWFGMADRAALVPGQPGQFELRGRSDRIVKLEE 360 Query: 373 KRISLTRIEQGLAASPLVADVRVIVHQGRRARQFIAAFVVPTAQGQAVLDGQGKLALNAA 432 KRISLTRIEQ L ASPLV DVRVIVHQGRRARQFIAAFVVP+A A+L QGKLALN A Sbjct: 361 KRISLTRIEQLLNASPLVEDVRVIVHQGRRARQFIAAFVVPSAPAHAMLAAQGKLALNTA 420 Query: 433 LKASLADAVEAIALPRLWRYLPALPLNPQGKVTLSALTALLESAGAAN-ASPPTLPRVTE 491 LKA LA A+E+IALPRLWRYLPALP+NPQGKVTLSALTALLES AA S PTLP+V+E Sbjct: 421 LKAVLAGAIESIALPRLWRYLPALPVNPQGKVTLSALTALLESPAAAPVVSAPTLPQVSE 480 Query: 492 LERTAEQVQLALEIPADLLYFQGHFPGSPILPGVVQTEWSLILGRRYFSLPPRFLGLQAL 551 LER++E VQLAL+IPADLLYF+GHFPGSPILPGVVQT+WSL LGRRYF LPP FLGLQAL Sbjct: 481 LERSSEHVQLALDIPADLLYFRGHFPGSPILPGVVQTDWSLALGRRYFDLPPHFLGLQAL 540 Query: 552 KFQRVITPGMRVVLELQHDAAKSQLAFRLHSEAGQHASGRFLLGAA 597 KFQRVITPGMRV+L+LQ +A KSQL+FRLHSE GQHASGRFLLGAA Sbjct: 541 KFQRVITPGMRVLLDLQFEAGKSQLSFRLHSEGGQHASGRFLLGAA 586 >gi|300311658|ref|YP_003775750.1| non-ribosomal peptide synthetase [Herbaspirillum seropedicae SmR1] Length = 9160 Score = 56.2 bits (134), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 129/481 (26%), Positives = 167/481 (34%), Gaps = 84/481 (17%) Query: 63 PGERIALFHHDSLHFAAALFGIWLAGKTAYLPSDTLPETCRQ-----------LRTEVDA 111 PG+ +AL AL W G AYLP DT R+ L E Sbjct: 4612 PGDIVALQLPRCAELVLALLATWRCG-AAYLPLDTDSPVARRQQILQAAKVHLLVMEAGQ 4670 Query: 112 FAGDFGAHCPAL-WPAADAVPDDALAPWPALRADFTGLVVYTSGSTGQAQAIPKQLGQMA 170 A A C A+ W P A AP P R D T V++TSGSTG + + Q+A Sbjct: 4671 SAEGLPADCTAVAWDELANSPVQASAPLPGSRMDATAYVLFTSGSTGAPKGVLIGHAQLA 4730 Query: 171 NEV-ATLEALFGSIAGQADVVATVSHQHIYGLLFKVLWALCAGRPIHAGSAFFPEDLQRM 229 V +AL + TV+ LF AL G +H SA D +R Sbjct: 4731 AYVQGVTQALRLDACRHWALTGTVAADLGNTALFG---ALANGACLHVASAEDMRDGERF 4787 Query: 230 TGPRPWL-------LLSSPAHLTRLPEQVTQPDPRQLRAIFSSGGPLPAQAARDAARLLG 282 +L L P+HL L E P L + PAQ AR A Sbjct: 4788 A---KFLRQHGIDALKIVPSHLDALLETPAPYLPATL--VLGGEATAPAQLARIRALAPQ 4842 Query: 283 HRPLEIYGSSET--GGIAWRQPESDLLQRREH-------WQAMPGVAVRI---------- 323 R YG +E G + R + +A+P VR+ Sbjct: 4843 CRVFNHYGPTECTVGVLVHRAATEAEAEAEADPGASLPLTEALPHAYVRVLDAQLRPTPA 4902 Query: 324 GAEGALEVRSPHLPDDQWFSMADRAVLVDD--LP----------------AAFELRGRSD 365 GA G L + L + DR +DD LP L GR+D Sbjct: 4903 GAIGQLWLGGAQLARGYLDAQQDREAFIDDPWLPGRRLYRSGDLAVLLPQGGVRLAGRAD 4962 Query: 366 RIVKLEEKRISLTRIEQGLAASPLVADVRVIVHQGRRARQFIAAFVVPTAQGQAVLDGQG 425 VK+ RI IE L P V V+ A A + GQA+ Sbjct: 4963 HQVKVRGFRIEPGEIEAALRTQPGVRQALVLA-----APDGALAACLAADHGQAL----- 5012 Query: 426 KLALNAALKASLADAVEAIALPRLWRYLPALPLNPQGKVTLSALTALLESAGAANASPPT 485 A +A+LA+ + A P W LP LP GKV ALLE G ++ P Sbjct: 5013 -----PAWRAALAEVLPAQMQPSRWLVLPELPRLGNGKVDRK---ALLEQLGRQDSGTPD 5064 Query: 486 L 486 + Sbjct: 5065 V 5065 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,927,585 Number of extensions: 83940 Number of successful extensions: 341 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 344 Number of HSP's successfully gapped: 3 Length of query: 597 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 506 Effective length of database: 1,188,985 Effective search space: 601626410 Effective search space used: 601626410 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)