BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0227.p (245 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein... 488 e-139 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 176 2e-45 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 164 5e-42 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 140 1e-34 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 115 4e-27 >gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 245 Score = 488 bits (1256), Expect = e-139, Method: Compositional matrix adjust. Identities = 240/245 (97%), Positives = 243/245 (99%) Query: 1 MTTSGAHSIFVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQTRV 60 MTT+GAHSIFVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQ RV Sbjct: 1 MTTTGAHSIFVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQVRV 60 Query: 61 LQFDVAHREQAAQALLADVEQHGCYYGVVCNAGITRDNAFPAMPGEDWDSVLRTNLDGFY 120 LQFDVA+REQAAQ LLADVEQHGCYYGVVCNAGITRDNAFPAMPGEDWDSVLRTNLDGFY Sbjct: 61 LQFDVANREQAAQVLLADVEQHGCYYGVVCNAGITRDNAFPAMPGEDWDSVLRTNLDGFY 120 Query: 121 NVLNPLVMPMVRRRAAGRIVTLASVSGLIGNRGQVNYSAAKAGVIGATKALALELAKRDI 180 NVLNPLVMPMVRRRAAGRIVTLASVSGLIGNRGQVNYSAAKAGVIGATKALALELAKRDI Sbjct: 121 NVLNPLVMPMVRRRAAGRIVTLASVSGLIGNRGQVNYSAAKAGVIGATKALALELAKRDI 180 Query: 181 TVNCVAPGLIDTDMTEDAPVDEILKLIPARRAGKPEEVAAAVAFLMSPDAAYITRQVISV 240 TVNCVAPGLIDTDMTEDAP+DEILKLIPARRAGKPEEVAAAVAFLMSPDAAYITRQVISV Sbjct: 181 TVNCVAPGLIDTDMTEDAPIDEILKLIPARRAGKPEEVAAAVAFLMSPDAAYITRQVISV 240 Query: 241 NGGLA 245 NGGLA Sbjct: 241 NGGLA 245 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 176 bits (446), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 112/240 (46%), Positives = 148/240 (61%), Gaps = 8/240 (3%) Query: 10 FVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQT-RVLQFDVAHR 68 VTG+SRGIGRAIA LAR+G ++ + AA + +AALG + R +V Sbjct: 12 LVTGASRGIGRAIATELARQGAKVIGTATSESGAA-AITEYLAALGPEAGRGAVLNVNDA 70 Query: 69 EQAAQALLADVEQ-HGCYYGVVCNAGITRDNAFPAMPGEDWDSVLRTNLDGFYNVLNPLV 127 E A+ AL+ V++ G +V NAGIT+D M E+WDSV+ TNL + ++ Sbjct: 71 E-ASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGRLSRAVL 129 Query: 128 MPMVRRRAAGRIVTLASVSGLIGNRGQVNYSAAKAGVIGATKALALELAKRDITVNCVAP 187 M++ R GRI+ + SV G GN GQ+NY+AAKAGV G ++ALA E+ R+ITVNCVAP Sbjct: 130 RGMMKART-GRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNITVNCVAP 188 Query: 188 GLIDTDMT---EDAPVDEILKLIPARRAGKPEEVAAAVAFLMSPDAAYITRQVISVNGGL 244 G IDTDMT + IL+ IP R G EE+A AVAFL SP AAYIT ++VNGG+ Sbjct: 189 GFIDTDMTRALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGTTLNVNGGM 248 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 164 bits (416), Expect = 5e-42, Method: Compositional matrix adjust. Identities = 94/238 (39%), Positives = 134/238 (56%), Gaps = 4/238 (1%) Query: 10 FVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQTRVLQFDVAHRE 69 +VTG GIG AI RL ++GY +V C D+ A + G + +V++ E Sbjct: 7 YVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQGFDIHASEGNVSNWE 66 Query: 70 QAAQALLADVEQHGCYYGVVCNAGITRDNAFPAMPGEDWDSVLRTNLDGFYNVLNPLVMP 129 A + G +V NAGITRD F M +DWD+V+ TNL+ +NV ++ Sbjct: 67 STKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNSLFNVTKQVIEG 126 Query: 130 MVRRRAAGRIVTLASVSGLIGNRGQVNYSAAKAGVIGATKALALELAKRDITVNCVAPGL 189 M R GRI+ ++SV+G G GQ NYS AKAG+ G T ALA E+A + +TVN V+PG Sbjct: 127 MCER-GFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNTVSPGY 185 Query: 190 IDTDMT---EDAPVDEILKLIPARRAGKPEEVAAAVAFLMSPDAAYITRQVISVNGGL 244 I TDM +++I+ IP +R G+PEE+A+ VA++ S + Y T S+NGGL Sbjct: 186 IGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFSLNGGL 243 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 140 bits (353), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 91/245 (37%), Positives = 136/245 (55%), Gaps = 6/245 (2%) Query: 4 SGAHSIFVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQTRVLQF 63 SGA VTG + GIG +I+ +LA +G+ VL DAA + A + A G + LQ Sbjct: 6 SGARVALVTGGAMGIGASISQQLAAKGH-TVLVADINLDAASKTADALVAAGHRAAPLQM 64 Query: 64 DVAHREQAAQALLADVEQHGCYYGVVCNAGITRDNAFPAMPGEDWDSVLRTNLDGFYNVL 123 D+ E A A + +G +V NAG+ R +F P E+W + N+ G Sbjct: 65 DLGSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAG 124 Query: 124 NPLVMPMVRRRAAGRIVTLASVSGLIGNRGQVNYSAAKAGVIGATKALALELAKRDITVN 183 MV+++ GRIV ++S+SG+ G+ Y +KA VIG T+ +A+ELA+ ITVN Sbjct: 125 QHAARLMVKQQW-GRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVN 183 Query: 184 CVAPGLIDTDMTE----DAPVDEILKLIPARRAGKPEEVAAAVAFLMSPDAAYITRQVIS 239 VAPG +DT MT+ + + +L+P RR G +E+A+AV +L + ++AYIT VI Sbjct: 184 SVAPGPVDTPMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGHVIP 243 Query: 240 VNGGL 244 V+GG Sbjct: 244 VDGGF 248 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 115 bits (287), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 83/242 (34%), Positives = 127/242 (52%), Gaps = 12/242 (4%) Query: 9 IFVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQTRVLQFDVAHR 68 + VTG++ GIG A A R + EG ++L A RDAA + +T + DV+H Sbjct: 17 VIVTGAASGIGEATARRFSDEGARVLL---ADRDAAALGKVFDSLPPERTAARETDVSHH 73 Query: 69 EQAAQALLADVEQHGCYYGVVCNAGITRDNAFPAMPGEDWDSVLRTNLDG-FYNVLNPLV 127 EQ Q + +E+ G +V +AG+ + + EDW V TN++G FY L Sbjct: 74 EQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATNVNGVFYGAREAL- 132 Query: 128 MPMVRRRAAGRIVTLASVSGLIGNRGQVNYSAAKAGVIGATKALALELAKRDITVNCVAP 187 P + + G IV +ASVSGL + Y+A+K V T+A+AL+ ++ + +N V P Sbjct: 133 -PHLEK-TRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGVRINAVCP 190 Query: 188 GLIDT----DMTEDAP-VDEILKLIPARRAGKPEEVAAAVAFLMSPDAAYITRQVISVNG 242 L T DM +D P +D+ + I R P E+AA + FL SPDA+++ + V+G Sbjct: 191 SLTHTAMTADMADDPPLLDKFAERIALGRGADPLEIAAVITFLASPDASFVNGVNLPVDG 250 Query: 243 GL 244 GL Sbjct: 251 GL 252 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 745,700 Number of extensions: 30100 Number of successful extensions: 266 Number of sequences better than 1.0e-08: 46 Number of HSP's gapped: 191 Number of HSP's successfully gapped: 46 Length of query: 245 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 162 Effective length of database: 1,226,865 Effective search space: 198752130 Effective search space used: 198752130 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)