BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0019.0220.p
         (358 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313015|ref|YP_003777107.1| maleylacetate reductase [Herbas...   598   e-172
gi|300309488|ref|YP_003773580.1| 4-hydroxybutyrate dehydrogenase...    99   4e-22

>gi|300313015|ref|YP_003777107.1| maleylacetate reductase
           [Herbaspirillum seropedicae SmR1]
          Length = 356

 Score =  598 bits (1542), Expect = e-172,   Method: Compositional matrix adjust.
 Identities = 311/350 (88%), Positives = 328/350 (93%)

Query: 7   HSFTYTSSAARVIFGAGSLQHLVRETDLLGARRALVLCTPGQRAEAERVAALIGERCAGI 66
            +FTYTS+AARVIFG GSLQHLVRET+LLGA+RALVLCTPGQRAEAERVAALIG RCAGI
Sbjct: 2   QAFTYTSAAARVIFGHGSLQHLVRETELLGAQRALVLCTPGQRAEAERVAALIGARCAGI 61

Query: 67  FDQAVMHVPIETARHAREVARELGADCAIAIGGGSTMGLGKAIALESELPILAIPTTYAG 126
           FDQAVMHVPIETARHAREVAR LGADCAIAIGGGSTMGLGKAIALESELPILAIPTTYAG
Sbjct: 62  FDQAVMHVPIETARHAREVARALGADCAIAIGGGSTMGLGKAIALESELPILAIPTTYAG 121

Query: 127 SEMTPIYGITEAGLKKTGRDARVLPRTVIYDPELSAGLPLKLSVTSGINAMAHAAEGLYA 186
           SEMTPIYG+TEAG KKTGRDARVLPRTVIYDP LS GLPL LSVTSGINAMAHAAEGLYA
Sbjct: 122 SEMTPIYGLTEAGRKKTGRDARVLPRTVIYDPALSLGLPLDLSVTSGINAMAHAAEGLYA 181

Query: 187 PDANPIMSLLAEEGLRALAEGLPQLVARSADLAARSQCLYGAWLCGTVLGNVGMSLHHKL 246
           PDANPIMSLLAEEGLRALAEGLPQL AR+ D+ ARS+CLYGAWLCGTVLGNVGMSLHHKL
Sbjct: 182 PDANPIMSLLAEEGLRALAEGLPQLAARADDIDARSRCLYGAWLCGTVLGNVGMSLHHKL 241

Query: 247 CHTLGGSYNLPHAETHTVILPHALAYNAPAAPEAMQRIARALGTDDAAQGVHALAARLQA 306
           CHTLGGSYNLPHA+THTVILPHALAYNA AAP+AMQRIARALG +DAAQGV+ALA  L A
Sbjct: 242 CHTLGGSYNLPHAQTHTVILPHALAYNAAAAPQAMQRIARALGVEDAAQGVYALARALHA 301

Query: 307 PLSLRELGMPYEELDRCTDIALQNAYANPRPLERTALRALLENAWQGRAP 356
           PLSL+ELGMPYE L +CT+IALQNAYANPRPL+R AL ALLE+AW GR P
Sbjct: 302 PLSLKELGMPYEALAQCTEIALQNAYANPRPLQREALHALLEDAWHGRPP 351


>gi|300309488|ref|YP_003773580.1| 4-hydroxybutyrate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 379

 Score = 99.4 bits (246), Expect = 4e-22,   Method: Compositional matrix adjust.
 Identities = 107/359 (29%), Positives = 165/359 (45%), Gaps = 42/359 (11%)

Query: 17  RVIFGAGSLQHLVRETDLLGARRALVLCTPGQRAEAERVAALIGERCAGI----FDQAVM 72
           +V F  G+L  L +E D LG ++ LV+   G R  A  +  ++G+   G+    +DQ   
Sbjct: 9   QVQFDYGALALLQQECDRLGIKKPLVVTDMGIR-NAGLLDKVLGQLKEGVGAVVYDQTPP 67

Query: 73  HVPIETARHAREVARELGADCAIAIGGGSTMGLGKAIA--------------LESEL--- 115
           +      R A  + R+ G D  +A+GGGS++ L K +A              +E  L   
Sbjct: 68  NPNEGAVRAAVALFRQHGCDGIVAVGGGSSIDLAKGVAVCGTHEGPLKSFALIEGGLANI 127

Query: 116 -----PILAIPTTYA-GSEM-TPIYGITEAGLKKTGRDARVLPRTVIYDPELSAGLPLKL 168
                P++AIPTT   GSE+      I + G K       ++P+  I DPEL+ GLP  +
Sbjct: 128 TAKTAPVIAIPTTAGTGSEVGRGAILILDDGRKVGIISPYLVPKLAICDPELTLGLPPLM 187

Query: 169 SVTSGINAMAHAAEGLYAPDANPIMSLLAEEGL-RALAEGLPQLVARSADLAARSQCLYG 227
           +  +G++A+AH  E   AP  NP    +A +GL RA A  + +      D  AR   +  
Sbjct: 188 TAATGMDAIAHCLETFMAPSFNPPADGIALDGLWRAWAH-IERATREPGDREARLN-MMS 245

Query: 228 AWLCGTVLGNVGMSLHHKLCHTLGG-SYNLPHAETHTVILPHALAYNAPAAP----EAMQ 282
           A + G +    G+   H L H+LGG +  L H   + + LP  +A+N  AA       M 
Sbjct: 246 ASMQGAMAFQKGLGCVHSLSHSLGGINPRLHHGTLNAIFLPAIIAFNESAASMVKDNKMA 305

Query: 283 RIARALGTDDAAQ---GVHALAARLQAPLSLRELGMPYEELDRCTDIAL--QNAYANPR 336
           R+A A+G    A+    +  ++ RL  P  L ELG+      +    AL   +   NPR
Sbjct: 306 RMAHAMGLGSGAEIGPAIREMSRRLGLPAGLGELGVTESMFPQIIQGALADHSHKTNPR 364


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.319    0.133    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,111,624
Number of extensions: 45874
Number of successful extensions: 210
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 208
Number of HSP's successfully gapped: 2
Length of query: 358
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 272
Effective length of database: 1,212,660
Effective search space: 329843520
Effective search space used: 329843520
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 131 (55.1 bits)