BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0219.p (269 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313016|ref|YP_003777108.1| 6-chlorohydroxyquinol-1,2-dioxy... 492 e-140 gi|300310639|ref|YP_003774731.1| catechol 1,2-dioxygenase [Herba... 139 2e-34 gi|300312960|ref|YP_003777052.1| catechol 1,2-dioxygenase [Herba... 133 2e-32 gi|300313273|ref|YP_003777365.1| protocatechuate 3,4-dioxygenase... 64 9e-12 >gi|300313016|ref|YP_003777108.1| 6-chlorohydroxyquinol-1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 296 Score = 492 bits (1266), Expect = e-140, Method: Compositional matrix adjust. Identities = 230/268 (85%), Positives = 252/268 (94%) Query: 1 MPNERLRQIMTSLVQHLHAFAREVQLTEEEWFEGIRFLTETGQLCSDKRQEFILLSDTLG 60 + ++RLR+IMTSLVQHLHAFAREV+LTE+EWF GI FLTETGQ+CSD+RQEFILLSDTLG Sbjct: 28 LTDQRLREIMTSLVQHLHAFAREVKLTEKEWFAGIDFLTETGQICSDQRQEFILLSDTLG 87 Query: 61 LSTLVMAQQNHKPAGCTEATVFGPFHVENSPVYPLGADISNGAKGQPCFVSGSVRGRDGQ 120 LSTLVMAQQNHKPAGCTEATVFGPFHVE++P YPLGADI+NGAKG+PCFVSGSVRGR+G+ Sbjct: 88 LSTLVMAQQNHKPAGCTEATVFGPFHVEDAPQYPLGADIANGAKGEPCFVSGSVRGRNGR 147 Query: 121 PVAHARMEVWQADEDGFYDVQYPDLDQLQARGTLHTDAQGRYHFRSILANSYPIPHDGPV 180 P+AHARMEVWQADE+GFYDVQY DLDQ +ARGTL TDA+GRYHFRSILAN YPIPHDGPV Sbjct: 148 PLAHARMEVWQADENGFYDVQYADLDQYRARGTLFTDAEGRYHFRSILANPYPIPHDGPV 207 Query: 181 GKMLETLGRHPWRPAHLHFRIQAEGYETLITHVFRRGDAYLESDAVFGVRSTLIADWPRH 240 G+MLE LGRHPWRPAHLHFRIQAEGYETLITHVFRR D YL+SDAVFGVRSTLIADWP H Sbjct: 208 GRMLEKLGRHPWRPAHLHFRIQAEGYETLITHVFRRDDVYLDSDAVFGVRSTLIADWPCH 267 Query: 241 EAGRAPDGSTCEQPFYTLDYDFILNPQR 268 AG APDG+ CEQPFYTLDYDF+LNP R Sbjct: 268 PAGTAPDGTQCEQPFYTLDYDFVLNPLR 295 >gi|300310639|ref|YP_003774731.1| catechol 1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 311 Score = 139 bits (351), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 88/272 (32%), Positives = 134/272 (49%), Gaps = 14/272 (5%) Query: 3 NERLRQIMTSLVQHLHAFAREVQLTEEEWFEGIRFLTETGQLCSDKRQEFILLSDTLGLS 62 N R +QI+ ++ L ++++T +E++ G+ ++ G E LLS LGL Sbjct: 27 NPRTKQIVHRILSDLFKTIEDLEITSDEYWAGVAYINRLGTA-----HEAGLLSPGLGLD 81 Query: 63 TLVMAQQNHKPA-----GCTEATVFGPFHVENSPVYPLGADISNGA--KGQPCFVSGSVR 115 + + + + A G T T+ GP +V +PV A + +G+ GQ + G+V Sbjct: 82 RFLDMRMDAEDAALGIAGGTPRTIEGPLYVAGAPVEQGFARLDDGSDKSGQTIIMHGTVY 141 Query: 116 GRDGQPVAHARMEVWQADEDGFYDVQYPDLDQ--LQARGTLHTDAQGRYHFRSILANSYP 173 G DG+P+ A++EVW A+ GFY P +Q R T+ TDAQGRY FRSI+ +Y Sbjct: 142 GADGKPMPGAQVEVWHANTKGFYSHFDPTGEQKPFNMRRTIITDAQGRYKFRSIMPTAYG 201 Query: 174 IPHDGPVGKMLETLGRHPWRPAHLHFRIQAEGYETLITHVFRRGDAYLESDAVFGVRSTL 233 P GP +L LGRH RPAH+HF + AEG+ L T + GD + D + R L Sbjct: 202 CPPAGPTQGLLNALGRHGNRPAHIHFFVTAEGHRKLTTQINIEGDPLIYDDFAYATREGL 261 Query: 234 IADWPRHEAGRAPDGSTCEQPFYTLDYDFILN 265 + + E PF + +D L Sbjct: 262 VPPLVERTDAASIKAEGLEGPFAEIVFDIKLT 293 >gi|300312960|ref|YP_003777052.1| catechol 1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 303 Score = 133 bits (334), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 83/273 (30%), Positives = 130/273 (47%), Gaps = 11/273 (4%) Query: 3 NERLRQIMTSLVQHLHAFAREVQLTEEEWFEGIRFLTETGQLCSDKRQEFILLSDTLGLS 62 N+R++ ++ LV+ + E+ + EE++ + +LT G K EF L++ LG Sbjct: 22 NQRVKTVVNRLVRDMFYTIEELDVQPEEFWAAVDYLTSAG-----KSGEFGLIAAGLGFE 76 Query: 63 TLVMAQQNHKPA-----GCTEATVFGPFHVENSPVYPLGADISNGAK-GQPCFVSGSVRG 116 + + + A G T T+ GP +V +P A + + G F+ G V Sbjct: 77 HFLDLRMDEAEARRGVQGGTPRTIEGPLYVSGAPEQKGFARMDKDPQPGDTLFMQGQVFD 136 Query: 117 RDGQPVAHARMEVWQADEDGFYDVQYPDLDQLQARGTLHTDAQGRYHFRSILANSYPIPH 176 G+P+A+A +EVW A+ G Y PD R ++ TD QGRY FRS + Y +P Sbjct: 137 EHGKPLANALVEVWHANHLGRYSFFDPDQSPFNLRCSIRTDEQGRYRFRSRVPVGYSVPP 196 Query: 177 DGPVGKMLETLGRHPWRPAHLHFRIQAEGYETLITHVFRRGDAYLESDAVFGVRSTLIAD 236 G ++L LGRH RPAH+HF + GY L T + GD YL D F R L+ + Sbjct: 197 GGATDRLLTKLGRHGSRPAHIHFFVTVPGYRKLTTQINIAGDPYLWDDFAFATREGLVPE 256 Query: 237 WPRHEAGRAPDGSTCEQPFYTLDYDFILNPQRT 269 + PFY ++++F L P++ Sbjct: 257 LKHVTDPAEIRKHEVDAPFYAIEFNFNLLPEQA 289 >gi|300313273|ref|YP_003777365.1| protocatechuate 3,4-dioxygenase beta chain protein [Herbaspirillum seropedicae SmR1] Length = 230 Score = 64.3 bits (155), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 47/148 (31%), Positives = 65/148 (43%), Gaps = 25/148 (16%) Query: 92 VYPLGADISNGAKGQP----CFVSGSVRGRDGQPVAHARMEVWQADEDGFY----DVQYP 143 + P D++ KG+P V+G V DG+PV ++ +EVWQ + G Y D Sbjct: 51 ILPHDMDLTKQGKGEPLGEKIVVTGRVVDEDGKPVRNSLLEVWQCNAAGRYFHKKDQHDA 110 Query: 144 DLD-QLQARGTLHTDAQGRYHFRSILANSYPIPHDGPVGKMLETLGRH--PWRPAHLHFR 200 LD G + TD GRY F SI YP G H WRPAH+HF Sbjct: 111 PLDPNFTGFGKMLTDDNGRYRFVSIKPGPYP-------------WGNHHKAWRPAHIHFS 157 Query: 201 IQAEGY-ETLITHVFRRGDAYLESDAVF 227 + Y + L+T ++ D D +F Sbjct: 158 LFGNVYAQRLVTQMYFPNDPLFAYDPIF 185 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 914,064 Number of extensions: 40241 Number of successful extensions: 81 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 77 Number of HSP's successfully gapped: 4 Length of query: 269 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 185 Effective length of database: 1,222,130 Effective search space: 226094050 Effective search space used: 226094050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)