BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0213.p (555 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313022|ref|YP_003777114.1| two component sensor histidine ... 944 0.0 gi|300313023|ref|YP_003777115.1| hypothetical protein Hsero_3729... 169 5e-43 gi|300311948|ref|YP_003776040.1| sensor histidine kinase/respons... 144 2e-35 gi|300312677|ref|YP_003776769.1| two component hybrid sensor his... 139 9e-34 gi|300310566|ref|YP_003774658.1| Hpt sensor hybrid histidine kin... 134 1e-32 >gi|300313022|ref|YP_003777114.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 944 bits (2439), Expect = 0.0, Method: Compositional matrix adjust. Identities = 465/556 (83%), Positives = 512/556 (92%), Gaps = 1/556 (0%) Query: 1 MDDRKHPPLPLRFARIDLRLALGLFSLLLVCGLWLGAFKELNTSRENHLQDARRDAQSLS 60 MDDRK PLRFARIDLR+AL LFSLLL+CGLWLGAFKEL SR N+L DARRDAQSLS Sbjct: 1 MDDRKQQSFPLRFARIDLRVALSLFSLLLICGLWLGAFKELQNSRNNYLDDARRDAQSLS 60 Query: 61 RLFLEHAYRTIEAADQAALYLRYRYAERGQSLDLATEISNGLVARNVYNLFTIVDAKGDV 120 RLFLEHAYRTIEAADQAALYLRYRYAERG +L+LATEI NGLVARNVYNLF+IVDA+GDV Sbjct: 61 RLFLEHAYRTIEAADQAALYLRYRYAERGNALNLATEIDNGLVARNVYNLFSIVDAQGDV 120 Query: 121 VLSSKPFTPLNLADREHVQVHMQGGEDKLFISKPVLGRVSKKWSLQLTRRINRPDGGFGG 180 VLSSKPFTP+NLADREHVQVHMQGG+D+LFISKPVLGRVSKKWSLQ+TRRINRPDGGFGG Sbjct: 121 VLSSKPFTPVNLADREHVQVHMQGGQDQLFISKPVLGRVSKKWSLQITRRINRPDGGFGG 180 Query: 181 VIVVSIDPQYFTSLYHQIDVGHHGVITLVGADGTARVRRTGDMDAMGEQVNGGKVYAAML 240 V+VVS+DPQYFT LYHQIDVGHHGVITLVGADG ARVRRTGDMDAMGEQV GGKVYAAML Sbjct: 181 VVVVSMDPQYFTRLYHQIDVGHHGVITLVGADGVARVRRTGDMDAMGEQVAGGKVYAAML 240 Query: 241 AQGSGVIDAFSRVDGRERIYAFHKLRDYPLYASVGIDIEERLAPYYRERNRTLTLATLIT 300 AQGSG I+A S++DGRER+YAF KLRDYPL+A+VGID++ERLAPYYRER RTL LA L++ Sbjct: 241 AQGSGTIEAVSKIDGRERLYAFQKLRDYPLFAAVGIDLKERLAPYYRERERTLMLAALLS 300 Query: 301 LAVLIFNAVLIWMAGSLVRSRQEAMQASQAKSRFLSNMSHEFRTPLNGVLGYSDALREEL 360 +AVL+FNAVL+W+AGSLVRSR+EA+QA+QAKSRFLSNMSHEFRTPLNGVLGYSDALREEL Sbjct: 301 VAVLVFNAVLLWLAGSLVRSRREALQANQAKSRFLSNMSHEFRTPLNGVLGYSDALREEL 360 Query: 361 GESPLAQYATAIHDSGNRLLELVESILEVTALEDRRVAVHREPENIRELVSQAIARHYQA 420 GESPLAQYA AIHDSGNRLL+LV +ILEVT LEDRRVAVH +P NIRELV QAIARHY Sbjct: 361 GESPLAQYAVAIHDSGNRLLDLVNAILEVTELEDRRVAVHLDPANIRELVGQAIARHYPQ 420 Query: 421 AQTKGLQLECRISDDVPQIIVCDQRKVLRVLENLIGNAVRYTDAGRVLVEVDRSS-DQLA 479 AQ KGL LECRI +DVPQI+VCD+RK+ RVL+NLI NAVRYTD GRV+VEV+RS QL Sbjct: 421 AQAKGLPLECRIGEDVPQILVCDERKIQRVLDNLISNAVRYTDHGRVMVEVERSGPQQLT 480 Query: 480 IRIKDTGIGIPAGQLDTIFEKFTQADDSERRAKDGAGLGLTIAQRLVTLMGGQLMLQSKQ 539 +RIKDTGIGI A Q + IFEKFTQADDS RR+++GAGLGLTIAQRLV LMGGQL+LQS+Q Sbjct: 481 MRIKDTGIGIAASQQNDIFEKFTQADDSPRRSQEGAGLGLTIAQRLVMLMGGQLVLQSEQ 540 Query: 540 HQGSTFSFTLPLQEPK 555 HQGSTFSFTLPLQEPK Sbjct: 541 HQGSTFSFTLPLQEPK 556 >gi|300313023|ref|YP_003777115.1| hypothetical protein Hsero_3729 [Herbaspirillum seropedicae SmR1] Length = 765 Score = 169 bits (428), Expect = 5e-43, Method: Compositional matrix adjust. Identities = 147/537 (27%), Positives = 245/537 (45%), Gaps = 45/537 (8%) Query: 49 LQDARRDAQSLSRLFLEHAYRTIEAA-----------DQAALYLRYRYAERGQSLDLATE 97 L D R D +LS + E+ +T+ +A +QA + + S++ + Sbjct: 46 LNDWRNDLSTLSTVLAENTAQTMSSARLVIDSIQADIEQANPTDQQQLQRHVGSVEFSQI 105 Query: 98 ISNGLVARNVYNLFTIVDAKGDVVLSSK--PFTPLNLADREHVQVHMQGGEDKLFISKPV 155 + + + + +A+G ++ S+ P P++L DR++ H F+ + V Sbjct: 106 MREKIRGLPFISGIGVANAEGRILALSRVYPAPPIDLTDRDYYLHHRSSDSTADFVGETV 165 Query: 156 LGRVSKKWSLQLTRRINRPDGGFGGVIVVSIDPQYFTSLYHQIDVGHHGVITLVGADGTA 215 L R + ++RR+N G +IV+ + +F + + + I L DG Sbjct: 166 LSRAGGVPTFYVSRRVNDRQGRLLAIIVIGLPCAFFETFFDNVTRDKPFSIVLARKDG-- 223 Query: 216 RVRRTGDMDAMGEQVNGGKVYAAMLAQGSGVIDA-----FSRVDGRERIYAFHK-LRDYP 269 + D + EQ G + LAQ + A S V + H+ +R++P Sbjct: 224 --KTLTSTDLLPEQ--GQRRSITPLAQLHPSMPADDEGRQSHVFMQSHWLGIHRPVRNWP 279 Query: 270 LYASVGIDIEERLAPYYRERNRTLTLATLITLAVLIFNAVLIWM--------AGSLVRSR 321 +Y +G+ E + E L L I+L LI VL W A R + Sbjct: 280 MYLEIGVTDEVYFGEWM-ESMYPLMLVAAISLVGLIGGFVLTWRQVRRREEDALLATRLK 338 Query: 322 QEAMQASQAKSRFLSNMSHEFRTPLNGVLGYSDALREELGESPLAQYATAIHDSGNRLLE 381 +EA A++AKS FL+ +SHE RTP+NG+LG S+ L E S YA +IH + L+ Sbjct: 339 EEADHANEAKSHFLAMVSHEIRTPMNGILGLSELLMESGLSSKQRDYADSIHGATGGLVR 398 Query: 382 LVESILEVTALEDRRVAVHREPENIRELVSQAIARHYQAAQTKGLQLECRISDDVPQ--I 439 ++ IL+ + +E ++ + + LV + + + Q K +Q + + D P+ Sbjct: 399 IINDILDFSKIESGKLDLEMVAFHPVRLVQELSDLYRPSLQRKDVQFDLHL--DCPENLS 456 Query: 440 IVCDQRKVLRVLENLIGNAVRYTDAGRVLVEVDRSSDQ-------LAIRIKDTGIGIPAG 492 +V D ++ +VL NL+ NA+++T AG V + + D+ L I DTGIGI Sbjct: 457 VVGDPARLKQVLGNLLSNAIKFTQAGHVQLSMSARQDESGPSRWRLFFAITDTGIGISPE 516 Query: 493 QLDTIFEKFTQADDSERRAKDGAGLGLTIAQRLVTLMGGQLMLQSKQHQGSTFSFTL 549 L +F F QAD S R G GLGL I++ LV LMGG + QS + + FSF + Sbjct: 517 ALQQLFRPFAQADSSISRRFGGTGLGLAISKNLVELMGGTIACQSIPGESTRFSFEI 573 >gi|300311948|ref|YP_003776040.1| sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1145 Score = 144 bits (363), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 84/238 (35%), Positives = 135/238 (56%), Gaps = 1/238 (0%) Query: 317 LVRSRQEAMQASQAKSRFLSNMSHEFRTPLNGVLGYSDALREELGESPLAQYATA-IHDS 375 L R+++ A A+ AKSRF++ MSHE RTPLN +LGY+ L + P + A I S Sbjct: 674 LQRAKEAAESANLAKSRFVAGMSHELRTPLNSILGYAQILHVDATIPPARKDAIGVIRRS 733 Query: 376 GNRLLELVESILEVTALEDRRVAVHREPENIRELVSQAIARHYQAAQTKGLQLECRISDD 435 G LL L++ +L++ +E ++ + + + + Q + A+ KGL S Sbjct: 734 GEHLLSLIDGLLDIAKIEAGKMRLASDELALEAFLEQIVGMFAPQAEQKGLHFRFDKSGR 793 Query: 436 VPQIIVCDQRKVLRVLENLIGNAVRYTDAGRVLVEVDRSSDQLAIRIKDTGIGIPAGQLD 495 +PQI+ DQ+++ ++L NL+GNAV++TD G V + + + + + DTGIGI + Sbjct: 794 LPQIVHADQKRLRQILINLLGNAVKFTDHGGVTLRLRYAREIAYFDVIDTGIGIADDDRE 853 Query: 496 TIFEKFTQADDSERRAKDGAGLGLTIAQRLVTLMGGQLMLQSKQHQGSTFSFTLPLQE 553 IF F ++ + R G GLGL I++ LV +MGG+L +QS+ QGS+F + L E Sbjct: 854 RIFLPFERSSAANLREDIGTGLGLAISRMLVHIMGGELSVQSQVGQGSSFHLKIYLPE 911 >gi|300312677|ref|YP_003776769.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1160 Score = 139 bits (349), Expect = 9e-34, Method: Compositional matrix adjust. Identities = 85/239 (35%), Positives = 137/239 (57%), Gaps = 6/239 (2%) Query: 317 LVRSRQEAMQASQAKSRFLSNMSHEFRTPLNGVLGYSDALREE----LGESPLAQYATAI 372 LV E +A+Q KS FL+NMSHE RTPLN L + L + L + + ++A I Sbjct: 461 LVEKATELERANQYKSEFLANMSHELRTPLNSTLILAKLLADNKQGNLSDEQV-RFAQTI 519 Query: 373 HDSGNRLLELVESILEVTALEDRRVAVHREPENIRELVSQAIARHYQAAQTKGLQLECRI 432 +GN LL L+ IL+++ +E ++ + E + +L + A AQ KGL + + Sbjct: 520 SSAGNDLLALINDILDLSKMEAGKLDIVPESFPLTKLADELSASFAITAQQKGLGFKVEV 579 Query: 433 SDDVPQIIVCDQRKVLRVLENLIGNAVRYTDAGRVLVEV-DRSSDQLAIRIKDTGIGIPA 491 VPQ + D +++ ++L+NL+ NA+++T+ G V + + + ++A ++DTGIGI Sbjct: 580 EPGVPQRMETDIQRLGQILKNLLSNAIKFTEKGEVSLRIYPDAGGRIAFAVRDTGIGIGE 639 Query: 492 GQLDTIFEKFTQADDSERRAKDGAGLGLTIAQRLVTLMGGQLMLQSKQHQGSTFSFTLP 550 Q IFE F QAD S R G GLGL+I++ L L+GG + + S+ +GS F+ +LP Sbjct: 640 DQHQFIFEAFRQADGSTHRKYGGTGLGLSISRDLAHLLGGDIRVTSRLGEGSIFTLSLP 698 >gi|300310566|ref|YP_003774658.1| Hpt sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 723 Score = 134 bits (338), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 84/241 (34%), Positives = 137/241 (56%), Gaps = 5/241 (2%) Query: 314 AGSLVRSRQEAMQASQAKSRFLSNMSHEFRTPLNGVLGYSDALREELGESPLAQYATAIH 373 A LV++++ A A + FLS MSHE RTP+N VLG + LR ++ A++A AI Sbjct: 70 AEKLVQAKEAADHAMLTRGAFLSTMSHEIRTPMNAVLGMAGLLRSTRLDTQQAEFAKAIE 129 Query: 374 DSGNRLLELVESILEVTALEDRRVAVHREPENIRELVSQAIARHYQAAQTKGLQLECRIS 433 DS + LL +V +L+ + ++ ++ + ++ ++V + A+ K ++L + Sbjct: 130 DSAHALLGIVNDVLDFSKIDVGKMDIAVVEFDLAKVVEASAEALSFKAREKRVRLLAHLD 189 Query: 434 DDVPQIIVCDQRKVLRVLENLIGNAVRYTDAGRVLVEVDR---SSDQLAIR--IKDTGIG 488 +P +V D ++ ++L NL+GNA+++T AG VL+ V +SD +R ++D+GIG Sbjct: 190 SRLPPRLVGDPGRIRQILLNLLGNALKFTHAGEVLLRVQALQVTSDHCRVRFEVRDSGIG 249 Query: 489 IPAGQLDTIFEKFTQADDSERRAKDGAGLGLTIAQRLVTLMGGQLMLQSKQHQGSTFSFT 548 + L +F F QAD S R G GLGL+I +RLV LMGG + ++S GS F F Sbjct: 250 MDEDTLRRLFSPFAQADSSVTRQYGGTGLGLSICKRLVELMGGSIGVESALGTGSCFWFE 309 Query: 549 L 549 L Sbjct: 310 L 310 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,606,462 Number of extensions: 66945 Number of successful extensions: 370 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 299 Number of HSP's successfully gapped: 45 Length of query: 555 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 465 Effective length of database: 1,193,720 Effective search space: 555079800 Effective search space used: 555079800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)