BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0201.p (259 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313027|ref|YP_003777119.1| two component transmembrane sen... 325 2e-90 gi|300312318|ref|YP_003776410.1| diguanylate cyclase with PAS/PA... 59 5e-10 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 55 7e-09 >gi|300313027|ref|YP_003777119.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 454 Score = 325 bits (834), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 152/185 (82%), Positives = 164/185 (88%) Query: 1 MGHSEALMANECASQRTHADDAPDTRALVKANARYRHIIGNTPAIIYSSVPSGDFQLTFV 60 MGH + ++ ++APD AL+KANARYRHIIGNTPAIIYSSVPSGDF+LTFV Sbjct: 1 MGHCDVFEVSQVCPLPAAKEEAPDFCALIKANARYRHIIGNTPAIIYSSVPSGDFRLTFV 60 Query: 61 SENATRLLGYEPSEMLDDPNFWFNHIHPDDTAAIFSSLAMLFVEDQKIYEYRFRTQDGSY 120 SENA+R+LGYEP EMLDDPNFWFNHIHPDDTA IFSSLAMLFVEDQKIYEYRFRT +GSY Sbjct: 61 SENASRVLGYEPCEMLDDPNFWFNHIHPDDTAQIFSSLAMLFVEDQKIYEYRFRTSNGSY 120 Query: 121 LWMHDTLRLIRDAEGTPLEVIGLMTDITGRKQMEDTLQRQAEEQRALIEQLKMAQAQLMQ 180 LWMHDTLRLIRD +GTP EVIGLMTDITGRK+MEDTLQRQAEEQR LIEQLKMAQAQLMQ Sbjct: 121 LWMHDTLRLIRDPDGTPQEVIGLMTDITGRKEMEDTLQRQAEEQRKLIEQLKMAQAQLMQ 180 Query: 181 SEKLA 185 SEKLA Sbjct: 181 SEKLA 185 >gi|300312318|ref|YP_003776410.1| diguanylate cyclase with PAS/PAC sensor protein [Herbaspirillum seropedicae SmR1] Length = 611 Score = 58.5 bits (140), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 35/96 (36%), Positives = 52/96 (54%), Gaps = 3/96 (3%) Query: 67 LLGYEPSEMLDDPNFWFNHIHPDDTAAIFSSLAMLFV--EDQKIYEYRFRTQDGSYLWMH 124 +LGYE E+ + IHPDD A + ++ F D E+R R QDGSY W+ Sbjct: 213 ILGYEDWELSSHLADAYQRIHPDDLAYVQQTIRQHFEARSDHYEVEHRIRCQDGSYKWIS 272 Query: 125 DTLRLI-RDAEGTPLEVIGLMTDITGRKQMEDTLQR 159 +++ RDA+G L +IG TDIT K++ + L + Sbjct: 273 SRGKVVARDAQGNALRMIGTTTDITEIKRLTEKLGK 308 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 54.7 bits (130), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 35/134 (26%), Positives = 61/134 (45%), Gaps = 2/134 (1%) Query: 38 IIGNTPAIIYSSVPSGDFQLTFVSENATRLLGYEPSEMLDDPNFWFNHI-HPDDTAAIFS 96 ++ N ++Y + + FVSE L GY P ++L + F + HP+D + + Sbjct: 13 LLVNLDGVVYRCRDDAQWTMEFVSEGCLALTGYLPEDLLLNQRLSFLELTHPEDRTLVRN 72 Query: 97 SLAMLFVEDQKI-YEYRFRTQDGSYLWMHDTLRLIRDAEGTPLEVIGLMTDITGRKQMED 155 + E ++I EYR DG+ W+ + I +A G + G + D+T RK+ Sbjct: 73 HVDACMRERRRIDIEYRIVRADGAVRWVWERGVGIYNANGQVEAIEGFLQDVTERKEAAQ 132 Query: 156 TLQRQAEEQRALIE 169 LQ R++ E Sbjct: 133 ALQEAERRYRSIFE 146 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.127 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 672,774 Number of extensions: 24349 Number of successful extensions: 118 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 117 Number of HSP's successfully gapped: 3 Length of query: 259 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 176 Effective length of database: 1,226,865 Effective search space: 215928240 Effective search space used: 215928240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)