BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0200.p (196 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313027|ref|YP_003777119.1| two component transmembrane sen... 338 2e-94 gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine k... 93 1e-20 gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine... 93 1e-20 gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine... 89 2e-19 gi|300311805|ref|YP_003775897.1| two component hybrid sensor his... 89 2e-19 >gi|300313027|ref|YP_003777119.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 454 Score = 338 bits (867), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 165/196 (84%), Positives = 176/196 (89%) Query: 1 VTELLAESNEGLQRVKNIVQSLKDFSRTGESNWQMADLHRGLDSTLNIANNELKYKVTIV 60 VTELLAESNEGLQRV+NIVQSLKDFSR GESNWQM DLHRGLDSTLNIANNELKYKV+IV Sbjct: 259 VTELLAESNEGLQRVRNIVQSLKDFSRIGESNWQMTDLHRGLDSTLNIANNELKYKVSIV 318 Query: 61 KQYGELPMIKCLASELNQVFMNLLVNAAQAIQERGSVTIRTERCGQRVAVCISDTGHGIA 120 KQYGELP++KCLASELNQVFMNLLVNAAQAI ERG+VTI T R G +V+V ISDTGHGIA Sbjct: 319 KQYGELPLVKCLASELNQVFMNLLVNAAQAISERGTVTICTARAGDKVSVSISDTGHGIA 378 Query: 121 PEHINRIFEPFFTTKPVGSGTGLGLSLSYGIIKKHGGDIHVSSEPGKGTTFRIELPIDPA 180 PE++NRIFEPFFTTKPVGSGTGLGLSLSYGI+KKHGGDI VSS PG GTTF IELPIDPA Sbjct: 379 PENLNRIFEPFFTTKPVGSGTGLGLSLSYGIVKKHGGDIRVSSAPGCGTTFTIELPIDPA 438 Query: 181 SAQPPSKASGEPGEAS 196 S S A+ GEAS Sbjct: 439 SVLAQSSATETSGEAS 454 >gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 845 Score = 93.2 bits (230), Expect = 1e-20, Method: Composition-based stats. Identities = 49/116 (42%), Positives = 70/116 (60%), Gaps = 14/116 (12%) Query: 75 ELNQVFMNLLVNAAQAIQERGSVTIRT-----------ER---CGQRVAVCISDTGHGIA 120 +L +NL +NA A+ G +TI T ER GQ V + ++D+G G+ Sbjct: 580 QLESALLNLCINARDAMPGGGRLTIETANKWLDDRWAHERDLPPGQYVCISVTDSGVGMP 639 Query: 121 PEHINRIFEPFFTTKPVGSGTGLGLSLSYGIIKKHGGDIHVSSEPGKGTTFRIELP 176 P+ + R F+PFFTTKP+G+GTGLGLS+ YG ++ GG + + SE G+GTT I LP Sbjct: 640 PDVLARAFDPFFTTKPIGAGTGLGLSMVYGFARQSGGQVRLYSEVGQGTTVSIYLP 695 >gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine kinase response regulator protein [Herbaspirillum seropedicae SmR1] Length = 688 Score = 93.2 bits (230), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 59/184 (32%), Positives = 89/184 (48%), Gaps = 15/184 (8%) Query: 8 SNEGLQRVKNIVQSLKDFSRTGESNWQMADLHRGLDSTLNIANNELKYKVTI-VKQYGEL 66 + ++R + Q L FSR + + ADL+R + + + + + G L Sbjct: 354 AQSAVKRAAALTQRLLAFSRRQTLDPKPADLNRIVAGMHELIGRSVGPAIAVETIAAGGL 413 Query: 67 PMIKCLASELNQVFMNLLVNAAQAIQERGSVTIRTER--------------CGQRVAVCI 112 ++ +NL +NA A+ G +TI T GQ V++ + Sbjct: 414 WHTFVDIGQMENALLNLCINARDAMPHGGRLTIETANRWLDDIGALQRGVPPGQYVSLSV 473 Query: 113 SDTGHGIAPEHINRIFEPFFTTKPVGSGTGLGLSLSYGIIKKHGGDIHVSSEPGKGTTFR 172 SDTG G++PE + R F+PFFTTKP G GTGLGLS+ YG + GG + + SE G+G Sbjct: 474 SDTGAGMSPEVVARAFDPFFTTKPTGQGTGLGLSMVYGFAGQSGGTVRIYSELGQGAMVC 533 Query: 173 IELP 176 I LP Sbjct: 534 IYLP 537 >gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 552 Score = 89.4 bits (220), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 45/118 (38%), Positives = 67/118 (56%), Gaps = 15/118 (12%) Query: 74 SELNQVFMNLLVNAAQAIQERGSVTIRTE---------------RCGQRVAVCISDTGHG 118 +++ +N+L+NA A+ RG V IRT + G V++ ++D G G Sbjct: 289 TQMEVALLNVLINARDAMNGRGRVRIRTANEDLADPDRAVQLGLKEGLYVSIAVTDDGPG 348 Query: 119 IAPEHINRIFEPFFTTKPVGSGTGLGLSLSYGIIKKHGGDIHVSSEPGKGTTFRIELP 176 IAP+ + R+ +PFFTTK G GTGLGLS+ YG +K+ GG + ++S GTT I P Sbjct: 349 IAPDILPRVMDPFFTTKDEGKGTGLGLSMVYGFVKQSGGSVRITSGAEGGTTVTIYFP 406 >gi|300311805|ref|YP_003775897.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1278 Score = 89.4 bits (220), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 47/118 (39%), Positives = 68/118 (57%), Gaps = 15/118 (12%) Query: 74 SELNQVFMNLLVNAAQAIQERGSVTIRTERC---------------GQRVAVCISDTGHG 118 +++ + +N +NA A+ G +T++ + G+ V + ISD G G Sbjct: 835 NQVENLIINTAINARDAMPSGGRLTVQIDNVRLEDPVFLSQADLAPGEYVELQISDNGAG 894 Query: 119 IAPEHINRIFEPFFTTKPVGSGTGLGLSLSYGIIKKHGGDIHVSSEPGKGTTFRIELP 176 ++PE +R FEPFFTTK G GTGLGLS++YG +K+ G I + SE GKGTT RI P Sbjct: 895 MSPEVRDRAFEPFFTTKLEGEGTGLGLSMAYGFVKQSKGHISIHSEQGKGTTIRIYFP 952 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.133 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 533,922 Number of extensions: 17790 Number of successful extensions: 145 Number of sequences better than 1.0e-08: 33 Number of HSP's gapped: 134 Number of HSP's successfully gapped: 33 Length of query: 196 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 116 Effective length of database: 1,241,070 Effective search space: 143964120 Effective search space used: 143964120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 128 (53.9 bits)