BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0197.p (1168 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313029|ref|YP_003777121.1| two component hybrid sensor his... 1457 0.0 gi|300313023|ref|YP_003777115.1| hypothetical protein Hsero_3729... 244 2e-65 gi|300311948|ref|YP_003776040.1| sensor histidine kinase/respons... 187 4e-48 gi|300310566|ref|YP_003774658.1| Hpt sensor hybrid histidine kin... 186 7e-48 gi|300312677|ref|YP_003776769.1| two component hybrid sensor his... 186 9e-48 >gi|300313029|ref|YP_003777121.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1524 Score = 1457 bits (3773), Expect = 0.0, Method: Compositional matrix adjust. Identities = 784/1193 (65%), Positives = 902/1193 (75%), Gaps = 64/1193 (5%) Query: 1 VVDSSPDAIVTTDARGLITSWNRGASDTLGLPEGRALGTALSTHAALRELDQQGSTRATS 60 VVDSSPDAIVTTDA G I+SWNRGA+D L +AL T L+ H LRELD + ++ A+ Sbjct: 367 VVDSSPDAIVTTDALGRISSWNRGAADYLPSSPAQALNTPLAQHPDLRELDARAASDASG 426 Query: 61 SGFSAGTGQQEEAHVVSLQGESIRYASALMFPLRDGAGRQIGKAFMLRDVTREKRIERQL 120 Q H+VSL G +RYASA +F L D AGR +GKAFMLRD+T EK IERQL Sbjct: 427 ---------QTTPHIVSLHGAPVRYASAQVFALHDRAGRAVGKAFMLRDITHEKNIERQL 477 Query: 121 HRINEELELTVQQRTHALHQAKTMLQTVLNVMPSLVGYWDKHLINRFANASYERVFGREH 180 RINEELE TVQQRT ALHQAKTMLQTVLNVMPSLV YWD+HLIN FANA+YER F RE Sbjct: 478 QRINEELEWTVQQRTQALHQAKTMLQTVLNVMPSLVAYWDRHLINHFANAAYERAFQREA 537 Query: 181 GEITGMYMLDLVGTELFESAMPHIDAVLAGEKQTFERTFTDAVNGYKHVLTTYIPDVNEQ 240 G++ G+YM DLVG LFE A+PHI VLAG +QTFER+ D V G +H+LTTYIP++ + Sbjct: 538 GQLNGVYMPDLVGPALFEKALPHIKGVLAGAQQTFERSVDDPVLGARHMLTTYIPEIGDL 597 Query: 241 GEVNGFYVVAHDVTEITHNRNQLSEALAQNQSLPDQVQQSNALLNNVLSSATGVAIIATA 300 GEV GFYVVAHDVTEITHNRNQLSE LAQNQSL DQVQ +N LLNNVLSSA GVAIIAT Sbjct: 598 GEVTGFYVVAHDVTEITHNRNQLSETLAQNQSLLDQVQDANTLLNNVLSSAKGVAIIATD 657 Query: 301 PDGVITLFNRGAERMLGYRAEDVIGQMKEETFSGRLNHPAIRAPAPLDEKAVSDLRYRRK 360 +G++TLFN GAE+MLGYRAEDV+G++KEE FS R P P + + ++L YRR Sbjct: 658 TEGLVTLFNSGAEQMLGYRAEDVVGKLKEEHFSSR---PEAALPG---QDSHTELLYRRH 711 Query: 361 DGVTLPVTRQQTSMLDHRQRHIGDLYIAMDMSLQRQQEAELAATRDQLMLAAEVAKLGVW 420 DG TLPV RQQTSM DHRQRH+G LYIA+D+SLQRQQ + LAA RDQL+LAAEVAKLGVW Sbjct: 712 DGATLPVARQQTSMFDHRQRHLGYLYIALDVSLQRQQASALAAARDQLVLAAEVAKLGVW 771 Query: 421 VWDRTGDTFEFNRQIAEIYGIGD-GRSGQVTTYEGWRRRVHPDDVGYVESALQAALAGTG 479 VWD+ D E+N Q+AEIYG GD G + +V+TY WRRRVHPDDV YVE L+A LA G Sbjct: 772 VWDKDSDVLEYNPQVAEIYGFGDRGGAPRVSTYADWRRRVHPDDVDYVEKTLRAGLAVDG 831 Query: 480 EFEPVFRVVRADGSIRFVQARAHVDRNGMGAPVRVIGINLDITQRIEFENNLVEARLKAE 539 + EPVFRVVR DG IR+VQARA+V R+G G PVRVI INLDITQRIEFENNLV+ARLKAE Sbjct: 832 DHEPVFRVVRDDGGIRYVQARAYVQRDGAGRPVRVICINLDITQRIEFENNLVDARLKAE 891 Query: 540 QANSAKSLFLASVSHEIRTPMNGVLGMIQLLQRTQPQPAQRDYLNKAEISESLXPEISAK 599 QANSAKSLFLASVSHEIRTPMNGVLGMIQLLQRTQP PAQ+DYL+KA EISAK Sbjct: 892 QANSAKSLFLASVSHEIRTPMNGVLGMIQLLQRTQPLPAQQDYLDKA--------EISAK 943 Query: 600 SLLNLLNDLLDFSKMDAQQVQLDLQAFSLEHFRHDLTAILAGNPPQEGVSLHIELDGDLP 659 SLLNLLNDLLDFSKMDA+Q+QLDLQAF LE F H+L A+LAG+PPQ GVSLHI LD LP Sbjct: 944 SLLNLLNDLLDFSKMDAKQLQLDLQAFELERFGHNLAAVLAGSPPQAGVSLHIALDPQLP 1003 Query: 660 QRVVGDVYRLQQVLVNLISNALKFTHLGRIDVALRRLAADNTRQVMLRVEVSDTGIGIAP 719 +R+VGD +RLQQVLVNL NALKFTH G IDV RL R V LRVEVSDTGIGIA Sbjct: 1004 RRLVGDAHRLQQVLVNLTGNALKFTHQGHIDVHFTRLDPLPGRTVALRVEVSDTGIGIAA 1063 Query: 720 ERLKDIFDAFTQAEASTTRRYGGTGLGLAISRHLVQLMGGQLQVSSEPGRGSRFWFDLPM 779 +RL DIFDAFTQAEASTTRRYGGTGLGLAIS+HLV LMGGQLQVSSEPGRGSRFWFDL + Sbjct: 1064 DRLDDIFDAFTQAEASTTRRYGGTGLGLAISKHLVGLMGGQLQVSSEPGRGSRFWFDLQL 1123 Query: 780 EIEDATPLS----------QARAADEHTQAEHAPAGETARE------LEGVQILLVEDNA 823 ++D + L Q A HT AP ETAR L+GVQILLVEDN Sbjct: 1124 AVDDDSVLGPAAADDAARPQPLQAASHTAGSRAP--ETARSAPDAQCLQGVQILLVEDNP 1181 Query: 824 VNRQVATGLLSLYGAHISCAENGSTGVAMACDPERHYDLILMDLQMPGIDGIEATRRIRR 883 VNRQVA+ LL+L GA ++CA++GS GVAMACDP RH+DL+LMDLQMPG+DGIEATRRIR Sbjct: 1182 VNRQVASELLALSGAQVTCADSGSMGVAMACDPARHFDLVLMDLQMPGMDGIEATRRIRG 1241 Query: 884 QQDLRHIPILAMTANASPEDRRRCIAAGMAGHIAKPIDVSTLVPTLLSHLQHDRSI--AA 941 Q +LR +PILAMTANASPED++RCI AGMAGHIAKPID++TL+P LL+HL H+R + AA Sbjct: 1242 QYELRGLPILAMTANASPEDKQRCIEAGMAGHIAKPIDIATLIPVLLAHLHHERPVPEAA 1301 Query: 942 QETLPAEATRVDVQPADRQQVDVYADLMRRYRQASDVYRTTLDAAGPELERLFADYDAAL 1001 + +P + DR V+ Y+ ++ R++QASD+YRTTLDAAGPELERL A Y AAL Sbjct: 1302 PQAVPQDLAD------DRLSVEDYSSILHRFQQASDIYRTTLDAAGPELERLLAQYCAAL 1355 Query: 1002 ------AQASPGGIEQAAHALKGLCATIGASGLAAYFAGIERVCRAGEG--VPSDRLFPA 1053 AQ + + QA+HAL+G+ ATIGA GLAAYF +E+ CR P Sbjct: 1356 AMSPEQAQEAMAALAQASHALRGVTATIGARGLAAYFGALEQRCRQAMAGRTPDLDWLER 1415 Query: 1054 WREELGAVRRDAGVVLQQLAMQALQGVSGSPGPAAPQAVAEPATATAEASDFPDRQALRA 1113 R E+ A + DA +V Q+L MQAL+ + A A AT ASDFPDRQ LR Sbjct: 1416 CRTEMDACQHDAMLVRQRLVMQALRPPASD------AGPAAAALATVAASDFPDRQRLRE 1469 Query: 1114 LLLSSNLEALDLAEAIGKRHGLMQQTRYQQLWELIEKLDFPAALAYLDDHFGE 1166 LLLS+NLEALDL E +GKRHGL QQ Y+QL LIEKLDF AAL LD HFG+ Sbjct: 1470 LLLSNNLEALDLIETLGKRHGLTQQEPYRQLNRLIEKLDFAAALTCLDHHFGD 1522 >gi|300313023|ref|YP_003777115.1| hypothetical protein Hsero_3729 [Herbaspirillum seropedicae SmR1] Length = 765 Score = 244 bits (624), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 163/446 (36%), Positives = 240/446 (53%), Gaps = 50/446 (11%) Query: 528 ENNLVEARLK--AEQANSAKSLFLASVSHEIRTPMNGVLGMIQLLQRTQPQPAQRDYLNK 585 E+ L+ RLK A+ AN AKS FLA VSHEIRTPMNG+LG+ +LL + QRDY + Sbjct: 329 EDALLATRLKEEADHANEAKSHFLAMVSHEIRTPMNGILGLSELLMESGLSSKQRDYADS 388 Query: 586 AEISESLXPEISAKSLLNLLNDLLDFSKMDAQQVQLDLQAFSLEHFRHDLTAILAGNPPQ 645 + L+ ++ND+LDFSK+++ ++ L++ AF +L+ + + + Sbjct: 389 IHGATG--------GLVRIINDILDFSKIESGKLDLEMVAFHPVRLVQELSDLYRPSLQR 440 Query: 646 EGVSLHIELDGDLPQRVVGDVYRLQQVLVNLISNALKFTHLGRIDVALR-RLAADNTRQV 704 + V + LD VVGD RL+QVL NL+SNA+KFT G + +++ R + Sbjct: 441 KDVQFDLHLDCPENLSVVGDPARLKQVLGNLLSNAIKFTQAGHVQLSMSARQDESGPSRW 500 Query: 705 MLRVEVSDTGIGIAPERLKDIFDAFTQAEASTTRRYGGTGLGLAISRHLVQLMGGQLQVS 764 L ++DTGIGI+PE L+ +F F QA++S +RR+GGTGLGLAIS++LV+LMGG + Sbjct: 501 RLFFAITDTGIGISPEALQQLFRPFAQADSSISRRFGGTGLGLAISKNLVELMGGTIACQ 560 Query: 765 SEPGRGSRFWFDLPMEIEDATPLSQARAADEHT--------------------------- 797 S PG +RF F++ + E +TP + + Sbjct: 561 SIPGESTRFSFEI-LCPEASTPAPEPVSEAAAPAPATALATTAPALPPAAEAPGTLMPPD 619 Query: 798 QAEHAPAGETARE------LEGVQILLVEDNAVNRQVATGLLSLYGAHISCAENGSTGVA 851 A P+ A L G +IL+ ED +NRQ+ LL+ G + ENG V Sbjct: 620 NAPQPPSNVDASATVLPSLLAGARILMAEDTEINRQLLRILLARKGCILQEVENGLQAVE 679 Query: 852 MACDPERHYDLILMDLQMPGIDGIEATRRIRRQQDLRHI---PILAMTANASPEDRRRCI 908 HYDL+LMD MP +DG +AT IR + R + PI+A+TA+A DR+RC+ Sbjct: 680 AI--RTGHYDLVLMDCMMPIMDGYQATAEIRAHEAARGLPRTPIIALTASAIEGDRQRCL 737 Query: 909 AAGMAGHIAKPIDVSTLVPTLLSHLQ 934 AGM +++KP L+ T+ LQ Sbjct: 738 DAGMDDYLSKPFTQVGLMQTVEKWLQ 763 >gi|300311948|ref|YP_003776040.1| sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1145 Score = 187 bits (475), Expect = 4e-48, Method: Compositional matrix adjust. Identities = 143/411 (34%), Positives = 218/411 (53%), Gaps = 37/411 (9%) Query: 531 LVEARLKAEQANSAKSLFLASVSHEIRTPMNGVLGMIQLLQ--RTQPQPAQRDYLNKAEI 588 L A+ AE AN AKS F+A +SHE+RTP+N +LG Q+L T P PA++D + Sbjct: 674 LQRAKEAAESANLAKSRFVAGMSHELRTPLNSILGYAQILHVDATIP-PARKDAIGVIRR 732 Query: 589 SESLXPEISAKSLLNLLNDLLDFSKMDAQQVQLDLQAFSLEHFRHDLTAILAGNPPQEGV 648 S + LL+L++ LLD +K++A +++L +LE F + + A Q+G+ Sbjct: 733 S--------GEHLLSLIDGLLDIAKIEAGKMRLASDELALEAFLEQIVGMFAPQAEQKGL 784 Query: 649 SLHIELDGDLPQRVVGDVYRLQQVLVNLISNALKFTHLGRIDVALRRLAADNTRQVMLRV 708 + G LPQ V D RL+Q+L+NL+ NA+KFT G + + LR R++ Sbjct: 785 HFRFDKSGRLPQIVHADQKRLRQILINLLGNAVKFTDHGGVTLRLRY-----AREIA-YF 838 Query: 709 EVSDTGIGIAPERLKDIFDAFTQAEASTTRRYGGTGLGLAISRHLVQLMGGQLQVSSEPG 768 +V DTGIGIA + + IF F ++ A+ R GTGLGLAISR LV +MGG+L V S+ G Sbjct: 839 DVIDTGIGIADDDRERIFLPFERSSAANLREDIGTGLGLAISRMLVHIMGGELSVQSQVG 898 Query: 769 RGSRFWFDLPMEIEDATPLSQARAADEHTQAEHAPAGETARELEGVQILLVEDNAVNRQV 828 +GS F + + E P + + D+ + AG + IL+V+D A R++ Sbjct: 899 QGSSFHLKIYLP-EVHVPRPRIKLEDQMS----GYAGPSR------HILVVDDQASQRRL 947 Query: 829 ATGLLSLYGAHISCAENGSTGVAMACDPERHY---DLILMDLQMPGIDGIEATRRIRRQQ 885 +L G +S A++G +AC + DL+L+D+ MP +DG R I R + Sbjct: 948 LKDMLMPLGFTVSEADSG-----LACMETLGHALPDLVLLDIAMPQMDGWSVARAI-RAR 1001 Query: 886 DLRHIPILAMTANASPEDRRRCIAAGMAGHIAKPIDVSTLVPTLLSHLQHD 936 L +PIL ++ANA R + + KP+ L+ + HLQ D Sbjct: 1002 GLAQLPILLVSANAFENLHERNEPPLVNDFVVKPVSYHELLGKIRQHLQLD 1052 >gi|300310566|ref|YP_003774658.1| Hpt sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 723 Score = 186 bits (473), Expect = 7e-48, Method: Compositional matrix adjust. Identities = 112/275 (40%), Positives = 168/275 (61%), Gaps = 18/275 (6%) Query: 514 VIGINLDITQRIEFEN-----NLVEARLKAEQANSAKSLFLASVSHEIRTPMNGVLGMIQ 568 + GI+ R+ EN LV+A+ A+ A + FL+++SHEIRTPMN VLGM Sbjct: 51 IWGIHALTLLRLLRENARTAEKLVQAKEAADHAMLTRGAFLSTMSHEIRTPMNAVLGMAG 110 Query: 569 LLQRTQPQPAQRDYLNKAEISESLXPEISAKSLLNLLNDLLDFSKMDAQQVQLDLQAFSL 628 LL+ T+ Q AE ++++ E SA +LL ++ND+LDFSK+D ++ + + F L Sbjct: 111 LLRSTRLDTQQ------AEFAKAI--EDSAHALLGIVNDVLDFSKIDVGKMDIAVVEFDL 162 Query: 629 EHFRHDLTAILAGNPPQEGVSLHIELDGDLPQRVVGDVYRLQQVLVNLISNALKFTHLGR 688 L+ ++ V L LD LP R+VGD R++Q+L+NL+ NALKFTH G Sbjct: 163 AKVVEASAEALSFKAREKRVRLLAHLDSRLPPRLVGDPGRIRQILLNLLGNALKFTHAGE 222 Query: 689 IDVALRRL--AADNTRQVMLRVEVSDTGIGIAPERLKDIFDAFTQAEASTTRRYGGTGLG 746 + + ++ L +D+ R +R EV D+GIG+ + L+ +F F QA++S TR+YGGTGLG Sbjct: 223 VLLRVQALQVTSDHCR---VRFEVRDSGIGMDEDTLRRLFSPFAQADSSVTRQYGGTGLG 279 Query: 747 LAISRHLVQLMGGQLQVSSEPGRGSRFWFDLPMEI 781 L+I + LV+LMGG + V S G GS FWF+L + + Sbjct: 280 LSICKRLVELMGGSIGVESALGTGSCFWFELGIPV 314 Score = 91.3 bits (225), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 57/120 (47%), Positives = 71/120 (59%), Gaps = 5/120 (4%) Query: 816 ILLVEDNAVNRQVATGLLSLYGAHISCAENGSTGVAMACDPERH-YDLILMDLQMPGIDG 874 ILLVEDN +N++VA L+ G A NG + RH Y L+LMD QMP +DG Sbjct: 478 ILLVEDNVMNQKVAVYQLNAMGYAADIAANGLEALERLA---RHRYALVLMDCQMPLLDG 534 Query: 875 IEATRRIRRQQ-DLRHIPILAMTANASPEDRRRCIAAGMAGHIAKPIDVSTLVPTLLSHL 933 E TRRIRR Q +L H I+AMTANA DR RC+ AGM ++ KP+ S L L +L Sbjct: 535 YETTRRIRRMQGELAHTTIVAMTANAMAGDRERCLEAGMDDYLTKPLLRSQLSAMLAHYL 594 >gi|300312677|ref|YP_003776769.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1160 Score = 186 bits (472), Expect = 9e-48, Method: Compositional matrix adjust. Identities = 137/430 (31%), Positives = 209/430 (48%), Gaps = 50/430 (11%) Query: 531 LVEARLKAEQANSAKSLFLASVSHEIRTPMNGVLGMIQLLQRTQPQPAQRDYLNKAEISE 590 LVE + E+AN KS FLA++SHE+RTP+N L + +LL + + + A+ Sbjct: 461 LVEKATELERANQYKSEFLANMSHELRTPLNSTLILAKLLADNKQGNLSDEQVRFAQTIS 520 Query: 591 SLXPEISAKSLLNLLNDLLDFSKMDAQQVQLDLQAFSLEHFRHDLTAILAGNPPQEGVSL 650 S + LL L+ND+LD SKM+A ++ + ++F L +L+A A Q+G+ Sbjct: 521 S-----AGNDLLALINDILDLSKMEAGKLDIVPESFPLTKLADELSASFAITAQQKGLGF 575 Query: 651 HIELDGDLPQRVVGDVYRLQQVLVNLISNALKFTHLGRIDVALRRLAADNTRQVMLRVEV 710 +E++ +PQR+ D+ RL Q+L NL+SNA+KFT G + + R+ D ++ V Sbjct: 576 KVEVEPGVPQRMETDIQRLGQILKNLLSNAIKFTEKGEVSL---RIYPDAGGRIAF--AV 630 Query: 711 SDTGIGIAPERLKDIFDAFTQAEASTTRRYGGTGLGLAISRHLVQLMGGQLQVSSEPGRG 770 DTGIGI ++ + IF+AF QA+ ST R+YGGTGLGL+ISR L L+GG ++V+S G G Sbjct: 631 RDTGIGIGEDQHQFIFEAFRQADGSTHRKYGGTGLGLSISRDLAHLLGGDIRVTSRLGEG 690 Query: 771 SRFWFDLPMEIEDATPLSQAR-------------------AADEHTQAEHAPAGETAREL 811 S F LP +S A + PA + L Sbjct: 691 SIFTLSLPPLYSSEQAVSNTAPEPTPAAAAAASTLQAAHVATAPDVRINRTPAAAAPQRL 750 Query: 812 EGVQ--------------ILLVEDNAVNRQVATGLLSLYGAHISCAENGSTGVAMACDPE 857 + + IL++ED+A + L G + + GVA A Sbjct: 751 QEISIDDDRDRIETSSRLILVIEDDAAFAMILRDLAREMGFQCVVTHSANEGVAAA--EM 808 Query: 858 RHYDLILMDLQMPGIDGIEATRRIRRQQDLRHIPI-LAMTANASPEDRRRCIAAGMAGHI 916 ILMD+ +P G+ +I+R RHIP+ + A+ S E R G G+ Sbjct: 809 YRPSAILMDMNLPDFSGLGVLDQIKRNPKTRHIPVHVVSVADYSHEAMER----GAIGYA 864 Query: 917 AKPIDVSTLV 926 KP+ L+ Sbjct: 865 LKPVQREELI 874 Score = 89.4 bits (220), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 46/120 (38%), Positives = 70/120 (58%), Gaps = 4/120 (3%) Query: 811 LEGVQILLVEDNAVNRQVATGLLSLYGAHISCAENGSTGVAMAC----DPERHYDLILMD 866 EG +IL+VED+ N + +L G + A NG + P+ DL+LMD Sbjct: 1032 FEGRRILVVEDDVRNIFALSSVLEPKGMKVEIARNGREALQALQRSQDKPDAPIDLVLMD 1091 Query: 867 LQMPGIDGIEATRRIRRQQDLRHIPILAMTANASPEDRRRCIAAGMAGHIAKPIDVSTLV 926 + MP +DG+ A R IR+Q + +PI+A+TA A +D+ +C+AAG +IAKP+DV L+ Sbjct: 1092 IMMPEMDGLTAMREIRKQAVWKRLPIIALTAKAMKDDQEKCLAAGANDYIAKPLDVEKLL 1151 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 3,456,341 Number of extensions: 147223 Number of successful extensions: 824 Number of sequences better than 1.0e-08: 49 Number of HSP's gapped: 782 Number of HSP's successfully gapped: 51 Length of query: 1168 Length of database: 1,619,870 Length adjustment: 96 Effective length of query: 1072 Effective length of database: 1,165,310 Effective search space: 1249212320 Effective search space used: 1249212320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 136 (57.0 bits)