BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0186.p (286 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herb... 549 e-157 gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herba... 129 2e-31 gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herb... 128 5e-31 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 127 9e-31 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 125 4e-30 >gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 369 Score = 549 bits (1414), Expect = e-157, Method: Compositional matrix adjust. Identities = 266/286 (93%), Positives = 276/286 (96%) Query: 1 MSDHKPEGLPPQHQTHQPGSEQEMHPRPHSAPAGQPGRRLAGKVALITGGDSGIGRAVAL 60 MS + + LP QHQ+HQPGSEQ+M PRPHSAPAG+PGRRLAGKVALITGGDSGIGRAVAL Sbjct: 84 MSKTEIDALPAQHQSHQPGSEQQMEPRPHSAPAGEPGRRLAGKVALITGGDSGIGRAVAL 143 Query: 61 AYAREGARLAIVYLDEEEDARQTQKDIQALDAPCLLIPGDVGSPEFCRSAVRSVMDAYGR 120 AYAREGARLAIVYLDEEEDARQTQKD+QALDAPCLL+PGDVGSPEFCRSAVR+VMDAYGR Sbjct: 144 AYAREGARLAIVYLDEEEDARQTQKDVQALDAPCLLLPGDVGSPEFCRSAVRAVMDAYGR 203 Query: 121 LDVLVNNAAQQYPAQSIEQISAEQLEHTFRTNIFSMFYLVKEAMPFLTRGARIINTTSVT 180 LDVLVNNAAQQYPAQSIE+IS QLEHTFRTNIFSMFY+VKEAMPFLTRGARIINTTSVT Sbjct: 204 LDVLVNNAAQQYPAQSIEEISQAQLEHTFRTNIFSMFYMVKEAMPFLTRGARIINTTSVT 263 Query: 181 AYRGSKHLVDYAATKGAIVAFTRSLSQQLAPAGIHVNAVAPGPIWTPLIPASFSEEEVAS 240 AYRGSKHLVDYAATKGAIVAFTRSLSQQLA AGIHVNAVAPGPIWTPLIPASFS EEVAS Sbjct: 264 AYRGSKHLVDYAATKGAIVAFTRSLSQQLASAGIHVNAVAPGPIWTPLIPASFSAEEVAS 323 Query: 241 FGKNVPLGRPGQPDEVAPAYVFLATEGASYMTGQVLHPNGGEIVNT 286 FGKNVPLGRPGQPDEVAPAYVFLATEGASYMTGQVLHPNGGEIVNT Sbjct: 324 FGKNVPLGRPGQPDEVAPAYVFLATEGASYMTGQVLHPNGGEIVNT 369 >gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 253 Score = 129 bits (325), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 88/247 (35%), Positives = 135/247 (54%), Gaps = 4/247 (1%) Query: 40 LAGKVALITGGDSGIGRAVALAYAREGARLAIVYLDEEEDARQTQKDIQALDAPCLLIPG 99 LAGKV I+G +GIG A A A+A G+ L + Y E++A +++A + + I Sbjct: 4 LAGKVVFISGASTGIGAAAARAFAARGSHLVVHYNSSEKEAEAVAAEVRAAGSKSITIGA 63 Query: 100 DVGSPEFCRSAVRSVMDAYGRLDVLVNNAAQQYPAQSIEQISAEQLEHTFRTNIFSMFYL 159 DV +AV + GR+DVL+NNA +E ++ E + N S+ Sbjct: 64 DVRDTSAINAAVAKAVAQMGRIDVLINNAGALVKRAPLETVTDELFDDIININARSVVAF 123 Query: 160 VKEAMPFLTR--GARIINTTSVTA-YRGSKHLVDYAATKGAIVAFTRSLSQQLAPAGIHV 216 + +P + G IIN TSV A + G + YAA+KG + TR ++++L I V Sbjct: 124 TRAVIPVMRAQGGGNIINVTSVAARHGGGPGALIYAASKGFVSTLTRGMAKELLADKIRV 183 Query: 217 NAVAPGPIWTPLIPASFSEEEVASFGKNVPLGRPGQPDEVAPAYVFLATEGAS-YMTGQV 275 NAVAPG I TP + E++ F K +P+GR G+PDE + A+++LA+E S Y+TGQ+ Sbjct: 184 NAVAPGVIMTPFQERFTTPEQLEGFRKTIPMGRIGEPDECSGAFLYLASEQMSGYVTGQI 243 Query: 276 LHPNGGE 282 + NGG+ Sbjct: 244 IDVNGGQ 250 >gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 128 bits (322), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 87/248 (35%), Positives = 124/248 (50%), Gaps = 6/248 (2%) Query: 40 LAGKVALITGGDSGIGRAVALAYAREGARLAIVYLDEEEDARQTQKDIQALDAPCLLIPG 99 +A KV L+TGG GIG A AL + G +A+ Y+ A IQA + + G Sbjct: 3 MADKVILVTGGSRGIGAATALLAGQRGYAVAVNYVTNRAAADAVVAQIQAAGGRAIAVGG 62 Query: 100 DVGSPEFCRSAVRSVMDAYGRLDVLVNNAAQQYPAQSIEQISAEQLEHTFRTNIFSMFYL 159 DV E +V GR+ LVNNA +E +SAE++ N+ Sbjct: 63 DVAREEDVLQLFATVDGELGRVTALVNNAGVLDHQSRLEDMSAERINRVLIANVTGSLLC 122 Query: 160 VKEAMPFLT-----RGARIINTTSVTAYRGS-KHLVDYAATKGAIVAFTRSLSQQLAPAG 213 +EA+ ++ +G I+N +S+ A G VDYAA+KGAI AFT L++++A G Sbjct: 123 AREAVKRMSTRHGGQGGAIVNLSSMAAKLGGPGEYVDYAASKGAIDAFTIGLAKEVAAEG 182 Query: 214 IHVNAVAPGPIWTPLIPASFSEEEVASFGKNVPLGRPGQPDEVAPAYVFLATEGASYMTG 273 I VNAV PG I+T + + V VP+ R G EVA A ++L +E ASY TG Sbjct: 183 IRVNAVRPGLIYTDIHASGGEAGRVDRLKDAVPMKRGGSAAEVANAILWLLSEEASYATG 242 Query: 274 QVLHPNGG 281 + +GG Sbjct: 243 TFIDVSGG 250 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 127 bits (319), Expect = 9e-31, Method: Compositional matrix adjust. Identities = 77/252 (30%), Positives = 135/252 (53%), Gaps = 11/252 (4%) Query: 40 LAGKVALITGGD--SGIGRAVALAYAREGARLAIVYLDEEEDARQTQKDIQALDAPCLLI 97 L KVA+ITGG +G+G A A A +GAR+ ++ L + + A + + + L + Sbjct: 3 LKDKVAVITGGAGINGLGYATARLMAEQGARVVVLDLAQADPAAAAAR----IGSQHLGL 58 Query: 98 PGDVGSPEFCRSAVRSVMDAYGRLDVLVNNAAQQYPAQSIEQISAEQLEHTFRTNIFSMF 157 DV C +A +++ +GR+D+LVNNA P +++E I+ + ++ Sbjct: 59 VADVTDKASCETAAAAILQQFGRIDILVNNAGITQPVKTLE-ITGADYDRILDVSLRGTL 117 Query: 158 YLVKEAMPFL--TRGARIINTTSVTAYRGSKHLVD--YAATKGAIVAFTRSLSQQLAPAG 213 Y+ + +P + + II +SV+A RG L Y+A K ++ R+++++ P G Sbjct: 118 YMSQAVLPAMRAQQNGSIICISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPEG 177 Query: 214 IHVNAVAPGPIWTPLIPASFSEEEVASFGKNVPLGRPGQPDEVAPAYVFLATEGASYMTG 273 I VN++ PG I T +I +EE+ A + +PL R G+ D++ A VFL ++ + Y TG Sbjct: 178 IRVNSITPGLIGTDIIKGKLTEEKKAEIAETIPLARLGRADDIGGACVFLGSDLSQYCTG 237 Query: 274 QVLHPNGGEIVN 285 L NGG +++ Sbjct: 238 ITLDVNGGMLIH 249 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 125 bits (314), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 83/252 (32%), Positives = 134/252 (53%), Gaps = 11/252 (4%) Query: 40 LAGKVALITGG--DSGIGRAVALAYAREGARLAIVYLDEEEDARQTQKDIQALDAPCLLI 97 L KVA+ITGG +G+G + A A +GAR+ I+ L+ E A + L A L + Sbjct: 3 LKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAAR----LGAQHLGL 58 Query: 98 PGDVGSPEFCRSAVRSVMDAYGRLDVLVNNAAQQYPAQSIEQISAEQLEHTFRTNIFSMF 157 DV C +A +V+ +GR+D+LVNNA PA+ +E I+ + + ++ Sbjct: 59 VADVTDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLE-ITGKDYDRILDVSLRGTL 117 Query: 158 YLVKEAMPFLT--RGARIINTTSVTAYRGSKHLVD--YAATKGAIVAFTRSLSQQLAPAG 213 Y+ + +P + + I+ +SV+A RG L Y+A K ++ R+++++ P G Sbjct: 118 YMSQAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPQG 177 Query: 214 IHVNAVAPGPIWTPLIPASFSEEEVASFGKNVPLGRPGQPDEVAPAYVFLATEGASYMTG 273 I VN + PG I T + S E+ A + +PLGR G PD+V VFLA++ + Y TG Sbjct: 178 IRVNCITPGLISTDINKDILSAEKKAEIAQTIPLGRLGGPDDVGGVCVFLASDLSQYCTG 237 Query: 274 QVLHPNGGEIVN 285 L NGG +++ Sbjct: 238 VTLDVNGGMLIH 249 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.133 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 980,260 Number of extensions: 43746 Number of successful extensions: 309 Number of sequences better than 1.0e-08: 51 Number of HSP's gapped: 208 Number of HSP's successfully gapped: 51 Length of query: 286 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 202 Effective length of database: 1,222,130 Effective search space: 246870260 Effective search space used: 246870260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)