BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0181.p (448 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313041|ref|YP_003777133.1| major facilitator superfamily p... 813 0.0 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 198 8e-52 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 183 2e-47 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 176 3e-45 gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 160 2e-40 >gi|300313041|ref|YP_003777133.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 436 Score = 813 bits (2099), Expect = 0.0, Method: Compositional matrix adjust. Identities = 415/439 (94%), Positives = 422/439 (96%), Gaps = 11/439 (2%) Query: 18 MTTSAESAEFTGALNESQHRPLTSSDYRTLGLSALGGTLEFYDFVIYVFFAAVVGKLFFP 77 MTTSAE FTGALNESQHRPL+SSDY+TLGLSALGGTLEFYDFVIYVFFAAVVGKLFFP Sbjct: 1 MTTSAE---FTGALNESQHRPLSSSDYKTLGLSALGGTLEFYDFVIYVFFAAVVGKLFFP 57 Query: 78 ADMPEWLRMLQTFGIFAAGYLARPVGGIIIAHYGDVIGRKRMFTLSIFLMAVPTLVIGLL 137 ADMPEWLRMLQTFGIFAAGYLARPVGGIIIAHYGDVIGRKRMFTLSIFLMAVPTLVIGLL Sbjct: 58 ADMPEWLRMLQTFGIFAAGYLARPVGGIIIAHYGDVIGRKRMFTLSIFLMAVPTLVIGLL 117 Query: 138 PTYASIGVAAPILLLLMRTLQGAAIGGEMPGAWVFVAEHVPANRYSFGVGSLTAGITGGI 197 PTYASIGVAAPILLLLMRTLQGAAIGGEMPGAWVFVAEHVPANRYSFGVGSLTAGITGGI Sbjct: 118 PTYASIGVAAPILLLLMRTLQGAAIGGEMPGAWVFVAEHVPANRYSFGVGSLTAGITGGI 177 Query: 198 LLGSLMGIFINSVYAPAEVAGYAWRIPFILGGVFGLVSVYLRRYLEETPVGKK------- 250 LLGSLMGIFINS + PAEVAGYAWRIPFILGGVFGLVSVYLRRYLEETPV ++ Sbjct: 178 LLGSLMGIFINSAFEPAEVAGYAWRIPFILGGVFGLVSVYLRRYLEETPVFRELAARRKE 237 Query: 251 -XELPLKTVLREHRAASVLTAGMTWCLSGAVVVVILMTPSYLQTVHHLPAKLVMQANCAG 309 ELPLKTVLREHRAASVLTAGMTWCLSGAVVVVILMTPSYLQTVHHL KLVMQANCAG Sbjct: 238 ANELPLKTVLREHRAASVLTAGMTWCLSGAVVVVILMTPSYLQTVHHLSPKLVMQANCAG 297 Query: 310 TLMLTIGCLAIGWLCDRIGTRASMIIGWGGLGLSTWWFYGQLPGSPESLIWGYALVGLFV 369 TLMLTIGCLAIGWLCDRIGTRASMIIGWGGLGLSTWWFYGQLPGSPESL+WGYALVGLFV Sbjct: 298 TLMLTIGCLAIGWLCDRIGTRASMIIGWGGLGLSTWWFYGQLPGSPESLVWGYALVGLFV 357 Query: 370 GSISLTPIVSTRAFPPPVRFTGLSFAYNMAYAVFGGLTPVLISLWIKADPQGPALYVIGL 429 GSISLTPIVSTRAFPPPVRFTGLSFAYNMAYAVFGGLTPVLISLWIKADPQGPALYVIGL Sbjct: 358 GSISLTPIVSTRAFPPPVRFTGLSFAYNMAYAVFGGLTPVLISLWIKADPQGPALYVIGL 417 Query: 430 SILGVLLAFVPMSSKGYKA 448 SILGVLLAFVPMSSKGY+A Sbjct: 418 SILGVLLAFVPMSSKGYRA 436 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 198 bits (503), Expect = 8e-52, Method: Compositional matrix adjust. Identities = 144/428 (33%), Positives = 219/428 (51%), Gaps = 35/428 (8%) Query: 18 MTTSAESAEFTGALNESQHRPLTSSDYRTLGLSALGGTLEFYDFVIYVFFAAVVGKLFFP 77 M S A+ +++ Q R R + + +G LE++DF +Y FFA ++ KLFFP Sbjct: 1 MQASTLPADGIVSVSNQQRR-------RAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFP 53 Query: 78 A--DMPEWLRMLQTFGIFAAGYLARPVGGIIIAHYGDVIGRKRMFTLSIFLMAVPTLVIG 135 D+ L + TFG+ G+ RPVGGI++ Y D +GRK +++I LMAV T +IG Sbjct: 54 TGNDLTSLLLAVATFGV---GFFMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIG 110 Query: 136 LLPTYASIGVAAPILLLLMRTLQGAAIGGEMPGAWVFVAEHVPANRYSFGVGSLTAGITG 195 L PTY SIG+ AP+++++ R LQG + GGEM A F+ E+ P ++ + A I Sbjct: 111 LAPTYESIGLFAPLIIVVARLLQGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGV 170 Query: 196 GILLGSLMGIFINSVYAPAEVAGYAWRIPFILGGVFGLVSVYLRRYLEETPV----GKKX 251 +LLG+ +G F+ + A +A + WR+PF+LG + G V Y+R +LEETP K Sbjct: 171 AVLLGAAVGTFVTASLDAAALASWGWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKT 230 Query: 252 ELPLKTVLREHRAASVLTAGMTWCLSGAVVVVILMTPSYLQTVHHLPAKLVMQANCAGTL 311 + PLK V+R + + M + V++ P+Y V LP A G L Sbjct: 231 DSPLKEVIRNFPRETSASFSMVILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGL 290 Query: 312 MLTIGCLAIGWLCDRIGTRASMIIGWGGLGLSTWW-FYGQLPGSPE-------SLIWGYA 363 + + +G L DRIG R ++ G L L + + + +P L++G Sbjct: 291 CIMVLAPIVGRLADRIGRR-PLLSGSAALILMLAYPMFAYINSAPSLSTLLIFQLVFG-T 348 Query: 364 LVGLFVGSISLTPIVS--TRAFPPPVRFTGLSFAYNMAYAVFGGLTPVLISLWIKA--DP 419 L+ + G PI++ + FP V TGLS AYN+A +FGG I+ I A Sbjct: 349 LIAAYTG-----PILAAFSELFPAKVLSTGLSVAYNLAVTIFGGFASFFITWLIAATGST 403 Query: 420 QGPALYVI 427 PA YV+ Sbjct: 404 MAPAFYVM 411 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 183 bits (465), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 122/405 (30%), Positives = 197/405 (48%), Gaps = 13/405 (3%) Query: 42 SDYRTLGLSALGGTLEFYDFVIYVFFAAVVGKLFFPADMPEWLRMLQTFGIFAAGYLARP 101 + R + + +G LE+YDF++Y FF+A++ +LFFPAD E+ +L F G+ RP Sbjct: 6 NKQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPAD-NEYTSLLVALATFGIGFFMRP 64 Query: 102 VGGIIIAHYGDVIGRKRMFTLSIFLMAVPTLVIGLLPTYASIGVAAPILLLLMRTLQGAA 161 VGG+++ Y D GRK + I LM + +I PTYA+IG+AAPI++++ R LQG A Sbjct: 65 VGGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGFA 124 Query: 162 IGGEMPGAWVFVAEHVPANRYSFGVGSLTAGITGGILLGSLMGIFINSVYAPAEVAGYAW 221 GGE + F+ E PA++ G + G+ MG + +P + + W Sbjct: 125 TGGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDSWGW 184 Query: 222 RIPFILGGVFGLVSVYLRRYLEETP-----VGKKXELPLK--TVLREHRAASVLTAGMTW 274 R+PF+LG + G V +++RR++ ET K P+K V++ + +++ G Sbjct: 185 RLPFVLGLLIGPVGLWIRRHMHETDDFLESANKPNAAPIKLLDVVKTNLRGVLVSMGQVI 244 Query: 275 CLSGAVVVVILMTPSYLQTVHHLPAKLVMQANCAGTLMLTIGCLAIGWLCDRIGTRASMI 334 + A VV++ P++ LP +LT G L DR+G R ++ Sbjct: 245 NGTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLGRRTVLL 304 Query: 335 IGWGGLGLSTWWFY---GQLPGSPESLIWGYALVGLFVGSISLTPIVSTRAFPPPVRFTG 391 L + + P P L+ L + + P + FP VR TG Sbjct: 305 YSSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPTRVRSTG 364 Query: 392 LSFAYNMAYAVFGGLTPVLISLWIKA--DPQGPALYVIGLSILGV 434 L+ AYN+A +FGG P +++ K P PA YV+ + +GV Sbjct: 365 LAVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAACIGV 409 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 176 bits (447), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 128/402 (31%), Positives = 199/402 (49%), Gaps = 12/402 (2%) Query: 45 RTLGLSALGGTLEFYDFVIYVFFAAVVGKLFFPADMPEWLRMLQTFGIFAAGYLARPVGG 104 R + S++G LE++D +IY FA V+ K FFP + + +L TF F + RP+G Sbjct: 19 RAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTG-DDSVSLLLTFATFGVSFFMRPLGA 77 Query: 105 IIIAHYGDVIGRKRMFTLSIFLMAVPTLVIGLLPTYASIGVAAPILLLLMRTLQGAAIGG 164 +++ Y D GRK LSI LM V T +I +P+YASIG+ AP + L + +QG + GG Sbjct: 78 VVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMIQGFSAGG 137 Query: 165 EMPGAWVFVAEHVPANRYSFGVGSL-TAGITGGILLGSLMGIFINSVYAPAEVAGYAWRI 223 E + F+ EH P R F + + GI+ +LL +L G +N++ P ++A + WR+ Sbjct: 138 EFGSSTAFLVEHAPHRRGFFSSWQVASQGIS--LLLAALFGAVLNNMLTPEQLASWGWRV 195 Query: 224 PFILGGVFGLVSVYLRRYLEETPVGKKXELPLKTVLRE---HRAASVLTAGMTWCLSGAV 280 PFI G + +Y+RR+L+E P K+ LR+ H+ +L + ++ Sbjct: 196 PFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTNAPLRDTFAHQKMRLLIGAGSVIMATVS 255 Query: 281 VVVILMTPSYLQTVHHLPAKLVMQANCAGTLMLTIGCLAIGWLCDRIGTRASMIIG---W 337 V + L P+Y LPA A L++ IG +G L D+IG MI + Sbjct: 256 VYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDKIGRTPFMITSSALY 315 Query: 338 GGLGLSTWWFYGQLPGSPESLIWGYALVGLFVGSISLTPIVSTRAFPPPVRFTGLSFAYN 397 L + F PG + L+ + L + P + FP R TG+S AYN Sbjct: 316 IVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVATRGTGMSLAYN 375 Query: 398 MAYAVFGGLTPVLISLWIKADPQGPALYVIGLSILGVLLAFV 439 +A +FGG ++I+ I D G L V I G +L+ + Sbjct: 376 IAVTIFGGFAGLIITWLI--DFTGDKLSVSYFVIFGAVLSLI 415 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 160 bits (405), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 127/419 (30%), Positives = 215/419 (51%), Gaps = 37/419 (8%) Query: 53 GGTLEFYDFVIYVFFAAVVGKLFFPADMPEWLRMLQTFGIFAAGYLARPVGGIIIAHYGD 112 G LE +DF +Y F+A+ + K FFP+D E++ ++ TFG F AG+L RP+G II+ Y D Sbjct: 22 GNFLEMFDFFLYGFYASYISKTFFPSD-NEFVSLILTFGTFGAGFLMRPLGAIILGSYID 80 Query: 113 VIGRKRMFTLSIFLMAVPTLVIGLLPTYASIGVAAPILLLLMRTLQGAAIGGEMPGAWVF 172 +GR++ +++ +MA+ TL+I +P YA+IGVAAPIL+L R LQG + G E+ G V+ Sbjct: 81 RVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVLTGRLLQGFSAGVELGGVSVY 140 Query: 173 VAEHVPANRYSFGVGSLTAGITGGILLGSLMGIFINSVYAPAEVAGYAWRIPFILGGVFG 232 +AE F V +A I+ +L+G ++ +P +++ + WRIPF +G + Sbjct: 141 LAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLSPVQISDWGWRIPFFIGCMIV 200 Query: 233 LVSVYLRRYLEETP--VGKKXELPLKTVLREHRAASVLTAGMTWCLSGAVVVVILMTPS- 289 +RR L+ET + +K ++ + W + A +++++MT Sbjct: 201 PAIFIIRRSLQETDEFLARKRHPSFGEIMS--------SIAQNWKIVVAGMMMVVMTTVS 252 Query: 290 -YLQTVHHLP-AKLVMQANCAGTLMLTIGCLAIGWLC---------DRIGTRASMIIGWG 338 YL TV+ K V++ + + +L++T C+ + C DR+G R +++ + Sbjct: 253 FYLITVYTPTFGKNVLKLSASDSLIVTF-CVGLSNFCWLPIMGALSDRVGRRP-VLVAFT 310 Query: 339 GLGLST-----WWFYGQLPGSPESLIWGYALVGLFVGSISLTPIVS-TRAFPPPVRFTGL 392 L L T W + S ++L+ + S + +V+ T P VR G Sbjct: 311 LLTLLTAYPVMSWLVSDI--SFKNLLITELWLSFLYASYNGAAVVALTEVMPAHVRTVGF 368 Query: 393 SFAYNMAYAVFGGLTPVLISLWI---KADPQGPALYVIGLSILGVLLAFVPMSSKGYKA 448 S AY++A A+FGG TP L+S W+ D P ++ ++ G+L V K A Sbjct: 369 SLAYSLATAIFGGFTP-LVSTWLIETSGDKAAPGYWMSFAAVCGLLATLVLYRGKSAAA 426 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.142 0.446 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,454,651 Number of extensions: 62986 Number of successful extensions: 372 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 339 Number of HSP's successfully gapped: 15 Length of query: 448 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 360 Effective length of database: 1,203,190 Effective search space: 433148400 Effective search space used: 433148400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)