BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0177.p (131 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313045|ref|YP_003777137.1| two component sensor histidine ... 216 7e-58 gi|300310565|ref|YP_003774657.1| response regulator containing a... 69 9e-14 gi|300312322|ref|YP_003776414.1| two component hybrid sensor his... 64 4e-12 gi|300311034|ref|YP_003775126.1| chemotaxis response regulator p... 63 8e-12 gi|300312287|ref|YP_003776379.1| chemotaxis cheY protein [Herbas... 58 2e-10 >gi|300313045|ref|YP_003777137.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 120 Score = 216 bits (549), Expect = 7e-58, Method: Compositional matrix adjust. Identities = 102/120 (85%), Positives = 111/120 (92%) Query: 12 LPLLLIVDDEIDITDTYAMLLELHGYRVVTAVHGADALTKAQQAWPDLVISDCMMPVMNG 71 +PLLLIVDDE+DITDTY+MLLELHGYRV+TA HGADAL KA+Q WPDLVISDCMMPVMNG Sbjct: 1 MPLLLIVDDEVDITDTYSMLLELHGYRVITACHGADALDKARQHWPDLVISDCMMPVMNG 60 Query: 72 LELCAALRALPGGATLPIILCSGAPERHDLSQSSHNLFLRKPVFFEQMLAEIQRLLTARP 131 LELCAALR LPGGA LP+ILCSGAPERHDLSQSSHNLFLRKPVFF+++L EI RLL RP Sbjct: 61 LELCAALRRLPGGAQLPVILCSGAPERHDLSQSSHNLFLRKPVFFDRLLEEIGRLLPGRP 120 >gi|300310565|ref|YP_003774657.1| response regulator containing a CheY-like receiver domain and an HD-GYP domain [Herbaspirillum seropedicae SmR1] Length = 348 Score = 69.3 bits (168), Expect = 9e-14, Method: Compositional matrix adjust. Identities = 41/124 (33%), Positives = 68/124 (54%), Gaps = 3/124 (2%) Query: 10 AALPLLLIVDDEIDITDTYAMLLELHGYRVVTAVHGADALTKAQQAWPDLVISDCMMPVM 69 + + +L++ DD+ +++ ML ++ R+ T AL Q+ PDL++ D MMP M Sbjct: 2 SNMNILIVDDDKTNLSLFSHMLGQVPQTRIDTCADARQALDWCQEHAPDLILLDYMMPGM 61 Query: 70 NGLELCAALRALPGGATLPIILCS---GAPERHDLSQSSHNLFLRKPVFFEQMLAEIQRL 126 +GL+ + RA+PG P+I+ + G RH Q S N FL KPV ++LA ++ + Sbjct: 62 DGLDFLSRFRAMPGQEMTPVIMITADMGLQVRHTALQMSANDFLSKPVNRVELLARVKNM 121 Query: 127 LTAR 130 L R Sbjct: 122 LAMR 125 >gi|300312322|ref|YP_003776414.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 775 Score = 63.9 bits (154), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 39/129 (30%), Positives = 62/129 (48%), Gaps = 4/129 (3%) Query: 3 GASPPQQAALPLLLIVDDEIDITDTYAMLLELHGYRVVTAVHGADALTKAQQAWPDLVIS 62 GA+P AA +L+VDD + + + LL GY+V AV G D + DLVI+ Sbjct: 643 GAAPTTTAARKKVLVVDDSLTVRELERKLLTNRGYQVTVAVDGMDGWNAVRADSFDLVIT 702 Query: 63 DCMMPVMNGLELCAALRALPGGATLPIILCSGAPERHD----LSQSSHNLFLRKPVFFEQ 118 D MP M+G+EL + +R +LP+++ S D L + + F + E Sbjct: 703 DIDMPRMDGIELVSLIRGAANLQSLPVMIVSYKDREEDRQRGLEAGADHYFTKSSFHDES 762 Query: 119 MLAEIQRLL 127 +L + L+ Sbjct: 763 LLQAVTDLI 771 >gi|300311034|ref|YP_003775126.1| chemotaxis response regulator protein [Herbaspirillum seropedicae SmR1] Length = 126 Score = 62.8 bits (151), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 41/117 (35%), Positives = 65/117 (55%), Gaps = 4/117 (3%) Query: 15 LLIVDDEIDITDTYAMLLELHGYRVVTAVHGADALTKAQQAW-PDLVISDCMMPVMNGLE 73 + +VDD + + LE+ G+ V TA G AL K Q + PDL+I+D MP M+GLE Sbjct: 7 IFLVDDSATMLMSVRSHLEMAGFAVDTAEDGVKALKKMQAGYKPDLLITDINMPNMDGLE 66 Query: 74 LCAALRALPGGATLPIILC---SGAPERHDLSQSSHNLFLRKPVFFEQMLAEIQRLL 127 L +RALPG +PI+ S + +R + ++ +L KPV +L ++++L Sbjct: 67 LIRNVRALPGFRFIPILTLTTESQSGKRDEGKKAGATGWLVKPVGGADLLKIVRQVL 123 >gi|300312287|ref|YP_003776379.1| chemotaxis cheY protein [Herbaspirillum seropedicae SmR1] Length = 121 Score = 58.2 bits (139), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 43/116 (37%), Positives = 59/116 (50%), Gaps = 8/116 (6%) Query: 15 LLIVDDEIDITDTYAMLLELHGYRVVTAVHGADALTKAQQAWPDLVISDCMMPVMNGLEL 74 +L VDD + L+ GY V AV G DAL KA+ DLV++D MP M+GL L Sbjct: 5 ILAVDDSGSLRQMVVFSLKAAGYNVTEAVDGVDALEKAKSQTFDLVLTDQNMPRMDGLTL 64 Query: 75 CAALRALPGGATLPIILCSGAPERHDLSQSSH-----NLFLRKPVFFEQMLAEIQR 125 +LR L A +PI++ + E D ++ N +L KP F Q L E+ R Sbjct: 65 IRSLRELTSYARVPILMLT--TEFSDEMKAKGKAAGANGWLVKP-FDPQRLTEVVR 117 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 372,859 Number of extensions: 13832 Number of successful extensions: 144 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 157 Number of HSP's successfully gapped: 9 Length of query: 131 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 56 Effective length of database: 1,264,745 Effective search space: 70825720 Effective search space used: 70825720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 125 (52.8 bits)