BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0171.p (971 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312702|ref|YP_003776794.1| VGR-related protein [Herbaspiri... 113 8e-26 gi|300310083|ref|YP_003774175.1| VGR-related type VI secretion s... 109 1e-24 gi|300313606|ref|YP_003777698.1| VGR-related type VI secretion s... 107 4e-24 gi|300310274|ref|YP_003774366.1| VGR-related protein [Herbaspiri... 89 1e-18 gi|300310071|ref|YP_003774163.1| VGR-related protein [Herbaspiri... 87 9e-18 >gi|300312702|ref|YP_003776794.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 824 Score = 113 bits (282), Expect = 8e-26, Method: Compositional matrix adjust. Identities = 107/464 (23%), Positives = 198/464 (42%), Gaps = 30/464 (6%) Query: 25 KFAVVNMQGFESLSKTFRFTLTLVTDDATIDFDQMLQNPATFSIFAPDGGGAVPYHGVLA 84 + + ++ E+LS+ + +TL L++DDA I D M+ ++ DG + G + Sbjct: 34 ELLINRLEASEALSRDYVYTLELLSDDAHIPLDAMMGKLLRVALTRADGT-QRHFTGYVE 92 Query: 85 GFDQLHRADGYVFYRAVLVPHLWRLSLYRVSEVYLNEQTIPSIIEGLLKGARLPASAFEL 144 F L G FY A LVP L L+ + ++ + ++ + + + + A ++ Sbjct: 93 RFAFLRSDGGIAFYEARLVPWLAFLASRHNNRIF-HHLSLEGLSAAIFRAYPMHAR-WDC 150 Query: 145 KLTGDYRPRSFVCQYQETHLDFLSRWMEKEGMYYWFDHGGRAEKLIIGDHRMMHAQDAIK 204 +L R+ + Q+ E+ + L R E G +Y ++H +L + D +A+ Sbjct: 151 QLRHGDPVRTEMFQFDESDSNLLHRRWEDAGWHYHYEHQADGHQLRLSDD-STYAKPIDG 209 Query: 205 VSYRPADELDTGAAADAVQGFVCKQKPLPYQVILQDFNYRKA-AVELKVSDIVSENGFGD 263 P D + + ++ P L ++++ A E++V + + Sbjct: 210 TGQVPFQRHGGAIEEDGMAQWSAVRQFQPSSTRLSSYDFKAARPQEVEVPTLNKQGAVLQ 269 Query: 264 VMIY----GENFRNEQEGKRYARLRAEEIACGGKLFAGDSTAVGLRSGYFMELAQHYR-- 317 V Y F N +G R +RLR EE ++F G L+ G L H+ Sbjct: 270 VEHYEYAGAYGFANRDDGDRQSRLRMEEFEAAAQVFEGTGNCRFLQPGRTFRLTGHFSGS 329 Query: 318 -----EDFNGKYLVTEITHEGSQAGALLEGIRSPYSQSGAGGETNYHNTFVAIRSDVQFR 372 D + + L+ + H + L + P+ Y N +R + +R Sbjct: 330 AASHDHDRDPELLIISVEHSATN-NYLQDSETPPF----------YANRVRCVRKRIPWR 378 Query: 373 PER--VTPKPRVAGTMNATIDGEGSGEYAELDEFGQYKVQLPFDQSDKNANKGSARVRMA 430 P R + + G AT+ G +GE +DE+G+ KVQ +DQ +N + SA +R+A Sbjct: 379 PGRSYYSQPTALLGIQTATVVGP-AGENLHVDEYGRVKVQFHWDQIGRNDERSSAWLRVA 437 Query: 431 TPYSGSNHGMHFPLHKNAEVLLSFTDGDPDQPVILGAVPNSENV 474 + +SG G+ V++ + G PD+P+I G+V N N+ Sbjct: 438 SSWSGGEQGLVSVPRIGQLVIVQWLGGHPDRPIITGSVVNQLNM 481 >gi|300310083|ref|YP_003774175.1| VGR-related type VI secretion system protein [Herbaspirillum seropedicae SmR1] Length = 944 Score = 109 bits (272), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 118/523 (22%), Positives = 212/523 (40%), Gaps = 82/523 (15%) Query: 41 FRFTLTLVTDDATIDFDQMLQNPATFSIFAPDGGGAVPYHGVLAGFDQLHRADGYVFYRA 100 +R L+ ++ +A I ++L P + GGA +HG + G Y Sbjct: 62 YRLQLSCLSLNADISLRKLLGQPVLLEL--DTDGGARAFHGHVTACRMEGANGGMARYLL 119 Query: 101 VLVPHLWRLSLYRVSEVYLNEQTIPSIIEGLL-----KGARLPASAFELKLTGDYRPRSF 155 + P L L R S V+ + + P+IIE + +G PA +L+ Y RS Sbjct: 120 TIEPWLSFLRHRRDSAVF-QDMSAPAIIEAVFADYRGQGRLQPAWRLDLQDASAYAKRSL 178 Query: 156 VCQYQETHLDFLSRWMEKEGMYYWFDHGGRAEKLIIGDHRMMHAQDAIKVSYRPA----- 210 QYQET L F++R M +EG++Y+ +H G + +G H ++ A S++P Sbjct: 179 ATQYQETDLAFVTRLMVEEGLFYYIEHQGDSNSASLGSHTVVIADH--NGSFKPNARSTV 236 Query: 211 --DELDTGAAADAVQGFVCKQKPLPYQVILQDFNYRKAAVELKVSDIVSENGFGDVMIYG 268 + + A D + + ++ V + ++YR+ AV + EN +++ Sbjct: 237 AFAQTNAVMAQDNIDRWRSVRRWQTNAVEILTWDYRQTAVRAASTASSGENAHDKLVLTS 296 Query: 269 EN----FRNE--QEGKRYARLRAEEIACGGKLFAGDSTAVGLRSG--YFMELAQHYREDF 320 + + NE ++ +R A + + I K+F G T G + + + Sbjct: 297 RDAPGQYINESPEQAQRQALNQMQAIEVRNKVFTGAGTVRSFLPGTTFTLTGHYDHDGGD 356 Query: 321 NGKYLVTEITHEG------------------------SQAGALLE-GIRSPYSQSGAGGE 355 + ++++ + H+ G+LL+ +RS +Q+ Sbjct: 357 DDQFVLLRVVHQAHNNLSADLKAGVTALLGQAQLDDHDHKGSLLDTALRSADTQA----R 412 Query: 356 TNYHNTFVAIRSDVQFRP-------ERVTPKPRVAGTMNATIDGEGSGEYAELDEFGQYK 408 Y N F AIR+ + +RP ER+ PKP+ G +A + G G+ D + + Sbjct: 413 PVYRNRFEAIRNKIPYRPLLVDAQGERLYPKPQAHGQQSAVVVG-AQGQAVHTDRDNRIR 471 Query: 409 VQLPFDQSDK------------------NANKGSARVRMA--TPYSGSNHGMHFPLHKNA 448 VQ + + ++ N G+ +A P +G+N G Sbjct: 472 VQFHWQRGERSHSGLSSPTPDDHVGAPANEQAGTWVRVLAGLAPTAGANWGGQAVPRVGQ 531 Query: 449 EVLLSFTDGDPDQPVILGAVPNSENVSLVNANSATENRISTKG 491 EVL+ F +GD D+PV++G + N V N A + S G Sbjct: 532 EVLIDFIEGDIDRPVVIGTLYNGRGVDNAQGNQAGQGAGSATG 574 >gi|300313606|ref|YP_003777698.1| VGR-related type VI secretion system protein [Herbaspirillum seropedicae SmR1] Length = 886 Score = 107 bits (268), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 136/569 (23%), Positives = 225/569 (39%), Gaps = 74/569 (13%) Query: 6 SRLMSNKRFEFVSTAFDRDKFAVVNMQGFESLSKTFRFTLTLVTDDATIDFDQMLQNPAT 65 +R+ ++R + ++ D + E L+ + T+ L+ I +Q++ P T Sbjct: 14 ARITQHRRLLQLDSSLGPDILLPQRLVATERLNDGYEVTIDLLATHTGIALEQLIAQPVT 73 Query: 66 FSIFAPDGGGAVPYHGVLAGFDQLHRADGYV-FYRAVLVPHLWRLSLYRVSEVYLNEQTI 124 I DG +P HG + +L +DG + F + L P L L + + ++ +++T Sbjct: 74 LWIRQHDGD-TLPLHGYVHTAKRLG-SDGELDFCQLSLSPWLHFLRFRKDARIWQDKRT- 130 Query: 125 PSIIEGLLKGARLPASAFELKLTGDYRPRSFVCQYQETHLDFLSRWMEKEGMYYWFDH-- 182 I+ + + +L Y PR + QY ET F+ R ME+EG WF H Sbjct: 131 EDILADVFNNHPQARGHYRFQLERPYTPRPYCTQY-ETDWHFVQRLMEEEG---WFAHHE 186 Query: 183 ---GGRAEKLIIGDHRM----MHAQDAIKVSYRPADELDTGAAADAVQGFVCKQKPLPYQ 235 G L+I D+ + Q P DELD A + K Sbjct: 187 QREDGSGHVLVITDNTWNLPGLPQQGIAFHGAAPRDELDKILHWSASRTLSSK------- 239 Query: 236 VILQDFNYRKAAVELKVSDIVSENGFGDVMIYGE--------------NFRNEQEGKRYA 281 +R + + K + E + YG+ +FR +++G R A Sbjct: 240 ------GWRGRSDDYKSPGMKKEAEEQVMPEYGQLPAQLEMYEYTGAYSFRLQEQGNRQA 293 Query: 282 RLRAEEIACGGKLFAGDSTAVGLRSGYFMELAQHYRE----DFNGKYLVTEI-------- 329 L+ E+ + ++ S L G + L +H + D ++++ I Sbjct: 294 DLQVEQWESSMQRYSAVSGVRNLACGRWFRLEEHPQHRAEPDRERQFVILAIDWFVENNL 353 Query: 330 --THE-----GSQAGALL---EGIRSPYSQSGAGGETNYH--NTFVAIRSDVQFRPERVT 377 +H+ GS GAL+ E IR + E H N F R + FRP R Sbjct: 354 PLSHQALDFPGSLKGALMAFKESIRRERQVAETDNEHTGHCFNRFEVQRRKLPFRPARAH 413 Query: 378 PKPRVAGTMNATIDGEGSGEYAELDEFGQYKVQLPFDQSDKNANKGSARVRMATPYSGSN 437 +P V A + G S E D + KVQ +D+ + S VR++ P +G Sbjct: 414 ARP-VMLPQTAIVVGPAS-EEVYTDHLDRIKVQFRWDRINPGNEAASCWVRVSYPNAGQY 471 Query: 438 HGMHFPLHKNAEVLLSFTDGDPDQPVILGAVPNSENVSLVNANSATENRISTK----GGN 493 G EV++SF +GDPD+PV+ G + N+E + N S + G N Sbjct: 472 WGAIQVPRIGQEVIVSFLNGDPDRPVVTGRLFNAEQRPQWHTNGRLSGVKSKEFGGTGFN 531 Query: 494 QMHMSDVKGKEVIWLNSPFHHSSIGIGSV 522 QM M D + I L S H+ + +G + Sbjct: 532 QMVMDDTPEQNRIHLYSTNTHAQLNLGHL 560 >gi|300310274|ref|YP_003774366.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 928 Score = 89.4 bits (220), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 127/553 (22%), Positives = 222/553 (40%), Gaps = 59/553 (10%) Query: 12 KRFEFVSTAFDRDKFAVVNMQGFESLSKTFRFTLTLVTDDATIDFDQMLQNPATFSIFAP 71 KR + + D M G E L + + +T+ LV+ I+ +++ P T + Sbjct: 32 KRILKLDSVLGPDVLLPQRMVGSERLGRGYDYTIDLVSTRDDIELKKLIAQPVTLWL-QQ 90 Query: 72 DGGGAVPYHGVLAGFDQLHRADGYVFYRAVLVPHLWRLSLYRVSEVYLNEQTIPSIIEGL 131 DG P HG + +L ++ + P L L YR + I+ + Sbjct: 91 DGSDYAPLHGYVHTARRLGSDGQLASFQIGMAPWLHFLK-YRKDARIWQDSKADDILADV 149 Query: 132 LKGARLPASAFELKLTGDYRPRSFVCQYQETHLDFLSRWMEKEGMYYWFDH--GGRAEKL 189 + ++ PRS+ QY ET F+ R ME+EG Y + + G L Sbjct: 150 FDQHAYARGNYRFEIVTPALPRSYCTQY-ETDWHFVQRLMEEEGWYAYHEQKPDGSGHVL 208 Query: 190 IIGDHRMMHAQDAI-KVSYRPA---DELDTGAAADAVQGFVCKQKPLPYQVILQDFNYRK 245 +I D+ A ++++ A DE++ A +G + + + +Y+ Sbjct: 209 VITDNTDQAKPLAPEQLAFHGAGTQDEVNRILHWSASRGLASTR------LATRTDDYKS 262 Query: 246 AAVELKVSD-IVSENGF--GDVMIY----GENFRNEQEGKRYARLRAEEIACGGKLFAGD 298 + + + ++ E+G G++ +Y ++ + +G R ARLR E+ K F G Sbjct: 263 PGDQKQTQERVLPEHGALPGELEVYEYSGPYSYAKQDDGDRQARLRVEQWESSMKRFMGV 322 Query: 299 STAVGLRSGYFMELAQH------YREDFNGKYLVTEITHEGSQA---------GAL---L 340 S A L +G + L H ++D + E E + G+L L Sbjct: 323 SGARTLAAGRWFTLTDHPAHRGDSKQDSQFMLIAVEWFIENNLPLSGTARDFPGSLKPQL 382 Query: 341 EGIRSPYSQSG--AGGET---NYHNTFVAIRSDVQFRPERVTPKPRVAGTMNATIDGEGS 395 + ++ +S AGG + N F R + +R KP V A + G Sbjct: 383 DAFKASMGRSNDDAGGNARTGHCFNRFEVQRRKLPYRSPFDHTKP-VMHPQTAVVVGP-Q 440 Query: 396 GEYAELDEFGQYKVQLPFDQSDKNANKGSARVRMATPYSGSNHGMHFPLHKNAEVLLSFT 455 GE D+ + KVQ +D+ + K S VR++ P +G N G E+++SF Sbjct: 441 GEEVFTDKLNRVKVQFRWDRINPGDEKASCWVRVSYPNAGENWGEVKVPRVGQELIISFL 500 Query: 456 DGDPDQPVILGAVPNSENVSLVNANSATENRIS--------TKGGNQMHMSDVKGKEVIW 507 +GDPD+PV G + N E + T+ R+S G NQ+ M D + + Sbjct: 501 NGDPDRPVATGRLFNQEQPPQWH----TDGRLSGLKSKEFGGNGFNQLVMDDTTSQNRVH 556 Query: 508 LNSPFHHSSIGIG 520 L S ++ + +G Sbjct: 557 LYSSQSNAQLNLG 569 >gi|300310071|ref|YP_003774163.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 954 Score = 86.7 bits (213), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 121/503 (24%), Positives = 193/503 (38%), Gaps = 64/503 (12%) Query: 24 DKFAVVNMQGFESLSKTFRFTLTLVTDDATIDFDQMLQNPATFSIFAPDGGGAVPYHGVL 83 + AV +G ES++ FRF + ++ +D Q + + + DGG +HG Sbjct: 40 ETLAVERFEGEESVNDNFRFDIDALSISTDLDLKQFIGAEISLRLLQADGGTRA-WHGYC 98 Query: 84 AGFDQLHRADGYVFYRAVLVPHLWRLSLYRVSEVYLNEQTIPSIIEGLLKGARLPASAFE 143 L G YR L L L R + T+ I +LK P + F Sbjct: 99 TQASWLGADGGLARYRLRLESFLAFLD-RRHDSFLFQDMTVTDIAAAVLK--EYPQANFS 155 Query: 144 LKLTGDYRPRSFVCQYQETHLDFLSRWMEKEGMYYWFDHGGRAEKLIIGDHRMMHAQDAI 203 L++T R QY+ET FL R + EG+ + F+H + + D HA+ + Sbjct: 156 LEVTQTLAKRDICTQYRETDYAFLRRILASEGLNFRFEHE-QDDATQQHDQSQPHARHKL 214 Query: 204 KVSYRPA--------DE------LDTGAAADAVQGFVCKQKPLPYQVILQDFNYRK-AAV 248 + R A DE + A+DA+ F + P V L ++ K ++ Sbjct: 215 VIFDRNAKAPALPGGDEYIRFHRVAAMEASDAITDFAAVRSVRPNGVALASWHPEKLSSP 274 Query: 249 ELKVSDIVSENGFGDVMIY----GENFRNEQEGKRYARLRAEEIACGGKLFAGDSTAVGL 304 + + + +Y ++F ++ +A+L + + K F G A L Sbjct: 275 SHEEGSTLDAGELPSLPVYDGTGQQDFADQDAAAAHAQLMLQALEMQNKRFEGAGAARQL 334 Query: 305 RSGYFMELAQHYR-EDFNGKYLVTEITHEG-SQAGALLEGIRSPYSQSGAGGET------ 356 +G L QH R + + V + H + A + I +P + + GG Sbjct: 335 AAGARFTLLQHERYPEGENAFKVVSVRHAARNNVSANITRILAPAAGALFGGSAAAERDD 394 Query: 357 -----NYHNTFVAIRSDVQFRP----ERVTPKPRVAGTMNATIDGEGSGEYAELDEFGQY 407 Y N+F AIR V P ER+ P R GT A + G D + Sbjct: 395 DLQAGTYRNSFTAIRDSVPMVPAAIAERLKPTAR--GTQTALVTGAADAAVT-TDRNHRV 451 Query: 408 KVQLPF-----------------DQSDKNANKGSAR---VRMATPYSGSNHGMHFPLHKN 447 KVQ + D D NAN + +R+A SG N G F Sbjct: 452 KVQFHWQRGKSPNPGGLRETGNLDDKDGNANGNDSSGTWIRVAEAQSGPNWGSQFIPRVG 511 Query: 448 AEVLLSFTDGDPDQPVILGAVPN 470 +EVL+ F +GD D+PV++ + N Sbjct: 512 SEVLVDFIEGDIDRPVVVAQLYN 534 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.129 0.362 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,772,002 Number of extensions: 113765 Number of successful extensions: 315 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 303 Number of HSP's successfully gapped: 7 Length of query: 971 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 877 Effective length of database: 1,174,780 Effective search space: 1030282060 Effective search space used: 1030282060 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 135 (56.6 bits)