BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0167.p (462 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310073|ref|YP_003774165.1| hypothetical protein Hsero_0738... 142 5e-35 gi|300313327|ref|YP_003777419.1| hypothetical protein Hsero_4040... 131 1e-31 >gi|300310073|ref|YP_003774165.1| hypothetical protein Hsero_0738 [Herbaspirillum seropedicae SmR1] Length = 448 Score = 142 bits (358), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 122/474 (25%), Positives = 204/474 (43%), Gaps = 38/474 (8%) Query: 1 MEDNKAIYWHQGMFMQPQHFQMADLHGQFRNKPLLESGLPHFWGVGELILSASAASNRII 60 M + + W +G+F++PQHFQ DL+ + R + + P+ WGV L + A +N + Sbjct: 1 MNHSNKVLWGEGLFLRPQHFQRQDLYHETRLRQTASALHPYCWGVSALQVDRDALNNNTL 60 Query: 61 EIERAQLLFPDRSYVEYPGNAAIAPRSFDSAWIEGDKPFNVYLGIKKLNDQEKNATQA-- 118 I L+F D + P D P + LG L Q A Sbjct: 61 RILELSLIFQDGEIYKAPDG--------------DDLPDPIELGEIALTQQSVTFYAALP 106 Query: 119 ------DSLAEAAAAPTRFASLSNTPEVPDLYSDGPPSQVRTLLHVVKVFFESELENLQG 172 + A+AA L + E PDL+++ +++ L V++ E+E + Sbjct: 107 AMKSFGSNFAQAAGGNQSNRYLLDNSETPDLFTEAAKAEMSYLKKAVRLVSENEPRD--S 164 Query: 173 YDLIPIARLVRDGDT-IKLADNFIPPCYTLGGSEVLMRTIRDIRDELSGRTRQLQEYKSP 231 IPI RL R + ++ NF+PP ++ + +L+ +R + D L + L Y Sbjct: 165 LVCIPIVRLRRSNSSGFEMDPNFVPPSLSVHAAPLLLLRLRRLMDALQAKVSAL--YGHH 222 Query: 232 REMQKA--EFDASYMVFLLALRSLNRASPYLFHLTETRQVHPWMAYGALRQLIGELSSFS 289 RE K+ EF + + L + + A L H +HP Y L L G L +FS Sbjct: 223 REPSKSVVEFRSGDITSFWLLHTASTAYASLSHYLHNPLLHPERLYEQLLTLGGSLMTFS 282 Query: 290 ERFNMLGEASDGTPGLPPYDHTELNKCFSRAHALISNLLNEITVGPEF-LAILEYRDGYH 348 S LP YDH + F+R +I LL+ + F +A+ E + YH Sbjct: 283 R--------SVALADLPAYDHQDPGPAFARLDGIIRELLDTVISSRYFAIALNETKPSYH 334 Query: 349 AAELPHTFFDRRNRFYLVVRTEQDPKWVADALLNDARLASPEDIPGLVSHALPGLELIHM 408 L D + FYL V + + D + ++ +P+D+ V ALPG++L+H Sbjct: 335 LGMLDSGKIDEKTNFYLAVNADLPAIELVDVVPLRFKIGAPDDVEKFVLAALPGVKLVHS 394 Query: 409 QVAPQGLPRRAYSYYFRIEQISQLWDSVERHGAIALQWLDAPDDLKAEIVVLRR 462 P LP R +YYF +E +++ + + +I + DLK E++ + + Sbjct: 395 PQVPAALPVRPDTYYFALENKGAMYERMLQAQSICIYVPAGIRDLKLELLAVTQ 448 >gi|300313327|ref|YP_003777419.1| hypothetical protein Hsero_4040 [Herbaspirillum seropedicae SmR1] Length = 448 Score = 131 bits (329), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 110/463 (23%), Positives = 195/463 (42%), Gaps = 20/463 (4%) Query: 1 MEDNKAIYWHQGMFMQPQHFQMADLHGQFRNKPLLESGLPHFWGVGELILSASAASNRII 60 M + + W +G+F++PQHFQ D + + R + P WG+ L + + +N + Sbjct: 1 MNQSNKVLWGEGLFLRPQHFQRQDHYHEARLHQTALTLNPFCWGISSLKVDHDSLNNNTL 60 Query: 61 EIERAQLLFPDRSYVEYPGNAAIAPRSFDSAWIE-GDKPFNVYLGIKKLNDQEKNATQAD 119 I +F D + P + P + I + Y + L N Sbjct: 61 RILELSAIFQDGEIYKAPDGDDL-PDPIELTEIPLSQQSVTFYAALPVLKSFGSN----- 114 Query: 120 SLAEAAAAPTRFASLSNTPEVPDLYSDGPPSQVRTLLHVVKVFFESELENLQGYDLIPIA 179 A A + + E PDL++D +++ L V++ ESE + IPI Sbjct: 115 -FASGVANHQSSRYIQHNIETPDLFTDAAKAELSYLKKAVRLASESEPRD--SLVCIPIV 171 Query: 180 RLVRDGDT-IKLADNFIPPCYTLGGSEVLMRTIRDIRDELSGRTRQLQEYKSPREMQKAE 238 RL R + +L +F+ P ++ + +L + +R + D L + L + E Sbjct: 172 RLRRSSSSGFELDPDFVAPSLSVHAAPLLFQRLRRLMDALQAKVSALYGHHREPSKNVVE 231 Query: 239 FDASYMVFLLALRSLNRASPYLFHLTETRQVHPWMAYGALRQLIGELSSFSERFNMLGEA 298 F + + L + + A L H +HP Y L L G L +FS R ML + Sbjct: 232 FRSGDITSFWLLHTASTAYARLSHYLNNPLLHPERLYEQLLALAGSLMTFS-RSLMLAD- 289 Query: 299 SDGTPGLPPYDHTELNKCFSRAHALISNLLNEITVGPEFLAIL-EYRDGYHAAELPHTFF 357 LPPY+H + F+R +I LL+ + F +L E + YH L Sbjct: 290 ------LPPYEHRDPGPPFARLDGIIRELLDTVISSRYFAIVLHESKPSYHMGMLDSGKI 343 Query: 358 DRRNRFYLVVRTEQDPKWVADALLNDARLASPEDIPGLVSHALPGLELIHMQVAPQGLPR 417 + + FYL V + + D + ++ +P+D+ V ALPG++L+H P LP Sbjct: 344 NEKTNFYLAVNADLPAIELVDVVPLRFKVGAPDDVEKFVLAALPGVKLVHSPQVPAALPV 403 Query: 418 RAYSYYFRIEQISQLWDSVERHGAIALQWLDAPDDLKAEIVVL 460 R +YYF +E +++ + + ++ + +LK E++ + Sbjct: 404 RPDTYYFTLESKGPMYERMLQAQSLCIYVPSGIRELKLELLAV 446 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,523,709 Number of extensions: 63924 Number of successful extensions: 175 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 166 Number of HSP's successfully gapped: 2 Length of query: 462 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 373 Effective length of database: 1,198,455 Effective search space: 447023715 Effective search space used: 447023715 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)