BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0166.p (230 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313328|ref|YP_003777420.1| type IV / VI secretion system t... 60 2e-10 gi|300310072|ref|YP_003774164.1| transmembrane protein [Herbaspi... 58 7e-10 >gi|300313328|ref|YP_003777420.1| type IV / VI secretion system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 257 Score = 60.1 bits (144), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 36/115 (31%), Positives = 56/115 (48%), Gaps = 3/115 (2%) Query: 25 DPAALSAQLDAAIAQARRAGQDAGHADADIEEALFAVLALADEIILATDWLGRAEWQRRL 84 D + +++ + + R + + DI A +A A DEIIL + + R W+RR Sbjct: 50 DDVSFESKMQKFLGEVERNAKKINASPDDIYAAKYAFCASVDEIILRSKFGIRPAWERRP 109 Query: 85 LQRRYFGITNAGVAFYTRLDAL---DAARSGVREVYFLCLSLGFAGRYGYDRNQK 136 LQ FG AG F+ +L+ L A EV+ +CL LGF G+Y + +K Sbjct: 110 LQLTMFGDQLAGENFFHKLEELRLHGNAHIQALEVFHMCLLLGFQGKYALEGPEK 164 >gi|300310072|ref|YP_003774164.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 57.8 bits (138), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 36/115 (31%), Positives = 57/115 (49%), Gaps = 3/115 (2%) Query: 25 DPAALSAQLDAAIAQARRAGQDAGHADADIEEALFAVLALADEIILATDWLGRAEWQRRL 84 D A+ + ++ + R + + D+ A +A A DEIIL +++ R W+RR Sbjct: 56 DEASFAIKMQKFLGDVERNAKKLDVSPEDVYAAKYAFCASVDEIILRSNFTIRDAWERRP 115 Query: 85 LQRRYFGITNAGVAFYTRLDAL---DAARSGVREVYFLCLSLGFAGRYGYDRNQK 136 LQ FG AG F+ +L+ L A EV+ +CL LGF G+Y + +K Sbjct: 116 LQLTMFGDQLAGENFFKQLEDLRVRGNAHVQALEVFHMCLLLGFQGKYALEGPEK 170 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.141 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 622,351 Number of extensions: 22917 Number of successful extensions: 103 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 101 Number of HSP's successfully gapped: 2 Length of query: 230 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 148 Effective length of database: 1,231,600 Effective search space: 182276800 Effective search space used: 182276800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 129 (54.3 bits)