BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0165.p (1312 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310093|ref|YP_003774185.1| transmembrane protein [Herbaspi... 156 1e-38 gi|300313314|ref|YP_003777406.1| transmembrane protein [Herbaspi... 150 8e-37 >gi|300310093|ref|YP_003774185.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1291 Score = 156 bits (394), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 143/544 (26%), Positives = 252/544 (46%), Gaps = 54/544 (9%) Query: 6 LLVLLALGCWGAALYMAWP-LWVALALFCGVIGVYFLSKFLRRVYIVMRSRSKL------ 58 L L +G L + W + +A+AL C +L +L R + V R+ KL Sbjct: 22 LAAFLFIGASTLELALTWAAVILAIALLC------WLGYWLYRRWKVRRAAGKLGEVLEQ 75 Query: 59 -AQQDVATRSSVAQAASPEALLRRKWKEAVATLRGSSLRRRGNP--LYVLPWYIVVGKSG 115 A Q VA S AQ AL R + EA+ T++ S L ++ LY LPWY+V+G Sbjct: 76 QATQSVAASSDGAQREEVAAL-RTRMLEAINTIKTSKLGQKSGSAALYELPWYMVIGNPA 134 Query: 116 TGKTTALTRARLSSPIQRVRQSGPVEQ----TQNYDWWYFDQAVVIDSAGRYVDAQDSEA 171 GK+TA+ + L P + G + T+N DW++ +++D+AGRY E Sbjct: 135 AGKSTAIANSGLQFPF--ADKGGKIVHGVGGTRNCDWFFTTDGILLDTAGRY---SVYEE 189 Query: 172 DRREWELGLDLLARYRPREGIDGLVLAIGTDRLANVSRDELA-EEGRVVRARIEQLIQMF 230 DR EW L LL RYR + I+G+++A+ L +R E A + +R R+++L + Sbjct: 190 DRNEWFSFLGLLKRYRKQAPINGIIIAVSIAELTG-NRPEFAINLAKNLRQRVQELTEKL 248 Query: 231 GKRFPIYVLVTKCDQLYGMEEWAKRMPENTLEQAMG--YLSDEIVGDRSAAQFLQGAFDS 288 P+YV+ TK D + G E+ + ++ G D + F FD Sbjct: 249 EVFAPVYVVFTKADLITGFNEFFLDTERSERDRVWGATLAYDRKRSGQEVVSFFDERFDE 308 Query: 289 IGARLQQLRIALVARMAGAAGEAASPELLLFPNELQNLRPKLELFLNACLADNPYLERPF 348 + A L+++ + A M+ GE +P +L FP E +++ L F+ +NP+ +P Sbjct: 309 LYAGLKEMSL---ASMSVKRGENMAPGVLTFPLEFSSIKGVLRSFVATLFEENPFQFKPV 365 Query: 349 LRGLFFSSGLQEGGAVSSLLGQVLP----PVASHPN----THAGLFLHDFFGRILPQDRF 400 RG +F+S +QEG VS+ ++ + P + G FLH+ F +++ D+ Sbjct: 366 FRGFYFTSAVQEGATVSASSQRIADRFGLALEHKPQREIFSQHGFFLHNLFKQVIFADK- 424 Query: 401 VSRPAELVNRWRQVTRNLGVVAWILIASAV-GIMI---TVSFVSNMETLSLVREKRPFDV 456 +LV ++ T+ A A A+ G+++ + S++ N + L+ V+ V Sbjct: 425 -----QLVAQYTSRTKIRMRYATFFAAIAILGVLLGGWSWSYMGNRQLLANVQADMDKAV 479 Query: 457 RFSGI---IEQDIPALQKVSDAVQLVERRNENWKTEWMVIATNMDDLEARLKLNFVSNFR 513 + ++ AL+ + D ++ ++R + + D LE +L+ + S + Sbjct: 480 KLQEKRLDLQSRFEALEILQDRIEQLDRYRASRPISVGMGLYQGDLLERKLREEYFSGIK 539 Query: 514 KYVL 517 + +L Sbjct: 540 EIML 543 >gi|300313314|ref|YP_003777406.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1266 Score = 150 bits (378), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 121/432 (28%), Positives = 204/432 (47%), Gaps = 45/432 (10%) Query: 2 LWLVL-----LVLLALGCWGAALYMAW------PLWVALALFCGVIGVYFLSKFLRRVYI 50 LW +L L LL A L++ P+W A+ LFC ++ + + +R + Sbjct: 4 LWKILSDTRVLTLLGFMALAAVLFIGAEQLELAPIWPAV-LFCALLLAWLALRRYQR-WK 61 Query: 51 VMRSRSKLA----QQDVATRSSVAQAASPE--ALLRRKWKEAVATLRGSSLRRRGNP--L 102 R+ +KL QQ ++AA + A LR++ EA+ T++ S L ++ L Sbjct: 62 AQRAATKLENVLEQQAAQPLPVDSEAARHDDVAALRQRMLEAINTIKTSKLGQKSGAAAL 121 Query: 103 YVLPWYIVVGKSGTGKTTALTRARLSSPIQRVRQSGPVEQ----TQNYDWWYFDQAVVID 158 Y LPWY+V+G GK+TA+ + L P + G + T+N DW++ +++D Sbjct: 122 YELPWYMVIGNPAAGKSTAIANSGLQFPF--ADKGGKIVHGVGGTRNCDWFFTTDGILLD 179 Query: 159 SAGRYVDAQDSEADRREWELGLDLLARYRPREGIDGLVLAIGTDRLANVSRDELA-EEGR 217 +AGRY E DRREW L LL RYR + I+G+++A+ L +R E A + Sbjct: 180 TAGRY---SVYEEDRREWFSFLGLLKRYRKQAPINGIIIAVSIAELTG-NRPEFAINLAK 235 Query: 218 VVRARIEQLIQMFGKRFPIYVLVTKCDQLYGMEEWAKRMPENTLEQAMG--YLSDEIVGD 275 +R R+++L + P+YV+ TK D + G E+ ++ G D Sbjct: 236 NLRQRVQELTEKLEVFAPVYVVFTKTDLITGFNEFFLDSERGERDRVWGATLAYDRKHSG 295 Query: 276 RSAAQFLQGAFDSIGARLQQLRIALVARMAGAAGEAASPELLLFPNELQNLRPKLELFLN 335 + + F FD + A L+++ + A MA GE +P +L FP E + L F+ Sbjct: 296 QEVSNFFDERFDELYAGLKEMSL---ANMAVKRGENLAPGVLTFPLEFSAFKGVLRSFVT 352 Query: 336 ACLADNPYLERPFLRGLFFSSGLQEGGAVSSLLGQVLP----PVASHPN----THAGLFL 387 +NP+ +P RG +F+S LQEG + + ++ + P + G FL Sbjct: 353 TLFEENPFQFKPVFRGFYFTSALQEGATLGASAQRIADRFGLTLEDQPQREVLSKQGFFL 412 Query: 388 HDFFGRILPQDR 399 H+ F +++ D+ Sbjct: 413 HNLFKQVIFADK 424 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 3,992,946 Number of extensions: 171544 Number of successful extensions: 731 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 725 Number of HSP's successfully gapped: 2 Length of query: 1312 Length of database: 1,619,870 Length adjustment: 97 Effective length of query: 1215 Effective length of database: 1,160,575 Effective search space: 1410098625 Effective search space used: 1410098625 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 136 (57.0 bits)