BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0163.p (268 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine k... 92 5e-20 gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine... 83 2e-17 gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine... 80 2e-16 gi|300311805|ref|YP_003775897.1| two component hybrid sensor his... 70 2e-13 gi|300313027|ref|YP_003777119.1| two component transmembrane sen... 66 3e-12 >gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 845 Score = 92.0 bits (227), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 78/261 (29%), Positives = 117/261 (44%), Gaps = 46/261 (17%) Query: 4 EVPPEEAVWLEVTDDGAGIAKEHLARLFEPFFTTRGGPGGKGTGLGLAIVAGIATEHDGG 63 ++PP + V + VTD G G+ + LAR F+PFFTT+ P G GTGLGL++V G A + G Sbjct: 620 DLPPGQYVCISVTDSGVGMPPDVLARAFDPFFTTK--PIGAGTGLGLSMVYGFARQSGGQ 677 Query: 64 VAVLTS-HEGTVFRLLLPVESQLAAQWARSADADAAQTPPSPISSTGEVITPLVPSWSGS 122 V + + +GT + LP Sbjct: 678 VRLYSEVGQGTTVSIYLPRH---------------------------------------Q 698 Query: 123 HDTASEMTSDDSTNVMEWQGQGQSVFLVDDDPALRPLYQRWLSGMGMEPIGFADPVEALN 182 HD + +D G G+ V +VDD+ ++R L LS G + + D AL Sbjct: 699 HDGDEQDEADALDETAAGDGNGEVVLVVDDEASIRMLVAEILSDAGYDVLQAEDGPGALR 758 Query: 183 ELTIEPRAVDLLVTDLDMP-QISGEALIVQARALRADLPVLLCSG--NHARLDEMGRQHD 239 L + R +DLL+TD+ +P ++G + RA R DL VL +G +A D + Sbjct: 759 ILQSKQR-IDLLITDVGLPGGLNGRQVADAGRAARPDLHVLFITGYAENAAFDNGHLERG 817 Query: 240 LVTLRKPFNEQGLRRAVQAAL 260 + + KPF + L R VQ+ L Sbjct: 818 MQLMTKPFKMEALARRVQSIL 838 >gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine kinase response regulator protein [Herbaspirillum seropedicae SmR1] Length = 688 Score = 83.2 bits (204), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 78/257 (30%), Positives = 118/257 (45%), Gaps = 46/257 (17%) Query: 5 VPPEEAVWLEVTDDGAGIAKEHLARLFEPFFTTRGGPGGKGTGLGLAIVAGIATEHDGGV 64 VPP + V L V+D GAG++ E +AR F+PFFTT+ P G+GTGLGL++V G A + G V Sbjct: 463 VPPGQYVSLSVSDTGAGMSPEVVARAFDPFFTTK--PTGQGTGLGLSMVYGFAGQSGGTV 520 Query: 65 AVLTS-HEGTVFRLLLPVESQLAAQWARSADADAAQTPPSPISSTGEVITPLVPSWSGSH 123 + + +G + + LP R P S VP+ GS Sbjct: 521 RIYSELGQGAMVCIYLP----------RHVGEGGVDDLALPEESH-------VPAAPGS- 562 Query: 124 DTASEMTSDDSTNVMEWQGQGQSVFLVDDDPALRPLYQRWLSGMGMEPIGFADPVEALNE 183 +++ +VDD+P +R + L +G + D AL Sbjct: 563 ---------------------KTILVVDDEPLVRMVTVEVLRDLGYSVLEAEDGPSALR- 600 Query: 184 LTIEPRAVDLLVTDLDMPQ-ISGEALIVQARALRADLPVLLCSG--NHARLDEMGRQHDL 240 + + +DLL+TD+ +P ++G L RA R DLPVL +G +A L+ + + Sbjct: 601 VVEACQEIDLLLTDVGLPNGMNGRQLADAIRAPRPDLPVLFVTGYAENAVLNHGHLERGM 660 Query: 241 VTLRKPFNEQGLRRAVQ 257 L KPF L R V+ Sbjct: 661 QVLTKPFAADVLARRVK 677 >gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 552 Score = 79.7 bits (195), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 74/252 (29%), Positives = 106/252 (42%), Gaps = 46/252 (18%) Query: 11 VWLEVTDDGAGIAKEHLARLFEPFFTTRGGPGGKGTGLGLAIVAGIATEHDGGVAVLTSH 70 V + VTDDG GIA + L R+ +PFFTT+ GKGTGLGL++V G + G V + + Sbjct: 338 VSIAVTDDGPGIAPDILPRVMDPFFTTK--DEGKGTGLGLSMVYGFVKQSGGSVRITSGA 395 Query: 71 EGTVFRLLLPVESQLAAQWARSADADAAQTPPSPISSTGEVITPLVPSWSGSHDTASEMT 130 EG G +T P+ E Sbjct: 396 EG------------------------------------GTTVTIYFPATQDQVGGGFEY- 418 Query: 131 SDDSTNVMEWQGQGQSVFLVDDDPALRPLYQRWLSGMGMEPIGFADPVEALNELTIEPRA 190 N + QG + + +VDD + L Q L +G P EAL +L R Sbjct: 419 ----DNRSQSQGGQEHILVVDDRLEVAELAQTMLESLGYRVSMVNSPAEAL-QLIRSGRP 473 Query: 191 VDLLVTDLDMP-QISGEALIVQARALRADLPVLLCSGNHAR-LDEMGRQHDLVTLRKPFN 248 VDLL TDL MP ++G L +A+ + + VLL +G + L+ G + L KP+ Sbjct: 474 VDLLFTDLIMPGSMNGVVLAREAKRMLPTIKVLLTTGYASESLERHGADGEFPVLDKPYR 533 Query: 249 EQGLRRAVQAAL 260 L R ++ L Sbjct: 534 HDELARKIRIVL 545 >gi|300311805|ref|YP_003775897.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1278 Score = 70.1 bits (170), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 63/226 (27%), Positives = 98/226 (43%), Gaps = 48/226 (21%) Query: 3 GEVPPEEAVWLEVTDDGAGIAKEHLARLFEPFFTTRGGPGGKGTGLGLAIVAGIATEHDG 62 ++ P E V L+++D+GAG++ E R FEPFFTT+ G+GTGLGL++ G + G Sbjct: 876 ADLAPGEYVELQISDNGAGMSPEVRDRAFEPFFTTK--LEGEGTGLGLSMAYGFVKQSKG 933 Query: 63 GVAVLTSH-EGTVFRLLLPVESQLAAQWARSADADAAQTPPSPISSTGEVITPLVPSWSG 121 +++ + +GT R+ P R D Sbjct: 934 HISIHSEQGKGTTIRIYFP----------RCLD--------------------------- 956 Query: 122 SHDTASEMTSDDSTNVMEWQGQGQSVFLVDDDPALRPLYQRWLSGMGMEPIGFADPVEAL 181 E D + G +++ +V+DDP L+ LS +G + AD E Sbjct: 957 ------EEVRIDEVRDLTVHGGDETILVVEDDPNLQLTVIDTLSSLGYRVLK-ADNGERA 1009 Query: 182 NELTIEPRAVDLLVTDLDMP-QISGEALIVQARALRADLPVLLCSG 226 E+ A+DL+ TD+ MP + L QAR L D+ +L SG Sbjct: 1010 LEILQSGVAIDLVFTDVVMPGSVPVTELAQQARLLVPDIEILFTSG 1055 >gi|300313027|ref|YP_003777119.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 454 Score = 65.9 bits (159), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 39/93 (41%), Positives = 57/93 (61%), Gaps = 5/93 (5%) Query: 9 EAVWLEVTDDGAGIAKEHLARLFEPFFTTRGGPGGKGTGLGLAIVAGIATEHDGGVAVLT 68 + V + ++D G GIA E+L R+FEPFFTT+ P G GTGLGL++ GI +H G + V + Sbjct: 364 DKVSVSISDTGHGIAPENLNRIFEPFFTTK--PVGSGTGLGLSLSYGIVKKHGGDIRVSS 421 Query: 69 S-HEGTVFRLLLPVE--SQLAAQWARSADADAA 98 + GT F + LP++ S LA A +A+ Sbjct: 422 APGCGTTFTIELPIDPASVLAQSSATETSGEAS 454 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.133 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 816,230 Number of extensions: 30953 Number of successful extensions: 236 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 260 Number of HSP's successfully gapped: 7 Length of query: 268 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 184 Effective length of database: 1,222,130 Effective search space: 224871920 Effective search space used: 224871920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 130 (54.7 bits)