BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0147.p (547 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis pro... 419 e-118 gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis tra... 410 e-115 gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis pro... 381 e-107 gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis tra... 365 e-102 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 364 e-102 >gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 576 Score = 419 bits (1077), Expect = e-118, Method: Compositional matrix adjust. Identities = 264/539 (48%), Positives = 358/539 (66%), Gaps = 6/539 (1%) Query: 1 MKLANFNIGVRLGGAFAVVLLLIMVTAMVGVRNQSSNNAEINSIVNEKYQLIALSNQIKN 60 MK+++ I VRLG FA +++++ V A VGV+N + + IVN++Y +AL QI + Sbjct: 2 MKISDMKISVRLGACFAALIVVMCVIAGVGVKNLLMVSDATDEIVNDRYVKVALGTQITD 61 Query: 61 NGYKANAVLSNILLATTPEASAKYMDEYAGLRKANTEAYKQLEGMLDSDKTKALYAEQTT 120 L N ++A PE KY+D +E ++E ++ + L A Sbjct: 62 KVNTGARSLRNAIMARNPEEQKKYLDRMDTYTSQVSEHLAEMEKRTNTPRGSELLAALKQ 121 Query: 121 ARSAYGVAVRKFFELMKTGERAEATTLFQGDLARLQTEYFVMVDKMVNSLAQEMVEDSRQ 180 AR+ Y K +L+ +R EAT +Q +YF V +MV+ + M + + + Sbjct: 122 ARADYSKERIKVVDLIHQQKRDEATAHLFDKTIPVQDKYFAAVKEMVSFQSSLMDKSAER 181 Query: 181 VAEDGHLAVIQMIVLAAVAVVLASVIGVLITRSITAPVRHAVVLAEAVAQGDLSYRLNAA 240 + A+ M+VL+ VAV+L+++ +LITRSIT P+ AV +A AVA GDL+ ++ A Sbjct: 182 ASAASSSAINIMLVLSGVAVLLSALCAMLITRSITRPLNEAVQVATAVAAGDLTVQIEAT 241 Query: 241 GKDEISRLLQALQHMSDNLHDTVSNVRNGTSAIDMAARELAQGNLSLSDRTEQQASSLEE 300 KDE LL AL+ M+DNLH V+ VR GT I+ A+RE+A GNL LS RTEQQA +LEE Sbjct: 242 SKDETGALLGALKSMNDNLHRIVAEVRAGTDTINTASREIATGNLDLSSRTEQQAGALEE 301 Query: 301 TASAMEQLTSAVRQNADNARQASELAVSASDVAVRGGASVEQVVNIMTAISTSSGKIADI 360 TASAME+LTS VRQNADNARQA+ LA +AS VA++GG+ V +VV M I+ +S KI DI Sbjct: 302 TASAMEELTSTVRQNADNARQANTLAETASQVAIQGGSVVGEVVQTMGQINDASRKIVDI 361 Query: 361 ITVIDGIAFQTNLLALNAAVEAARAGEHGRGFAVVASEVRGLAQRSAVAAKEIKSLIEGS 420 I+VIDGIAFQTN+LALNAAVEAARAGE GRGFAVVASEVR LAQRSA AAKEIKSLI+ S Sbjct: 362 ISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSAAAAKEIKSLIDAS 421 Query: 421 VGHVKAGSNIVEQTVATIDEVIASIKHATDLITDISASSKEQSEGIEQINQAISQMDQMT 480 V V++GS +V Q AT++EV+AS+K TD++ +I+A+S EQS+GIEQINQAI QMD++T Sbjct: 422 VERVESGSRLVGQAGATMEEVVASVKRVTDVVAEITAASGEQSDGIEQINQAIVQMDEVT 481 Query: 481 QENSALVEQNAAASQALQSQAEQLSALMGRFRLSELLANGQAGGHAASPRKGPREKVIQ 539 Q+N+ALVEQ AAA+Q+LQ Q+ +LS +G FRL G H SP P ++ +Q Sbjct: 482 QQNAALVEQAAAAAQSLQEQSGRLSDTVGIFRL------GNDHAHQPSPLSAPVQRSVQ 534 >gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 410 bits (1053), Expect = e-115, Method: Compositional matrix adjust. Identities = 259/517 (50%), Positives = 352/517 (68%), Gaps = 6/517 (1%) Query: 1 MKLANFNIGVRLGGAFAVVLLLIMVTAMVGVRNQSSNNAEINSIVNEKYQLIALSNQIKN 60 MKL + I VRL FAV++ ++ + VG+++ SS + IV ++Y+ IAL+ +I++ Sbjct: 1 MKLKDIKISVRLAVCFAVLIGVMCLITGVGMKSLSSISKSSALIVEDRYEKIALAVEIRD 60 Query: 61 NGYKANAVLSNILLATTPEASAKYMDEYAGLRKANTEAYKQLEGMLDSDKTKALYAEQTT 120 N A L N LL E + +Y+D A TEA ++E +L + + + L+ Sbjct: 61 NVNGAARNLRNALLGRNLEEATRYLDRSAANSAKTTEALGKMEKILSTARGRELFQSIQE 120 Query: 121 ARSAYGVAVRKFFELMKTGERAEATTLFQGDLARLQTEYFVMVDKMV---NSLAQEMVED 177 ARSAY K EL+K ++ EA L + Q +YF ++D+ V SL ++ V + Sbjct: 121 ARSAYNTPRDKLRELIKQQKKEEAVELLFTQVIPAQDKYFQVLDQFVAFQKSLMEQSVTE 180 Query: 178 SRQVAEDGHLAVIQMIVLAAVAVVLASVIGVLITRSITAPVRHAVVLAEAVAQGDLSYRL 237 R + A+ M+ L+AVA+ L + L+TRSIT P+ AV +A AVA GDL+ ++ Sbjct: 181 GRDTTQS---AIAMMLGLSAVAIALCVIAAWLVTRSITRPLNEAVSVASAVAAGDLTVQI 237 Query: 238 NAAGKDEISRLLQALQHMSDNLHDTVSNVRNGTSAIDMAARELAQGNLSLSDRTEQQASS 297 A KDE LL AL+ M+D+LH VS VR G+ I+ A+ E+A GNL LS RTE+QA + Sbjct: 238 GATSKDETGALLAALKSMNDSLHRIVSEVRQGSDTINTASAEIATGNLDLSSRTEEQAGA 297 Query: 298 LEETASAMEQLTSAVRQNADNARQASELAVSASDVAVRGGASVEQVVNIMTAISTSSGKI 357 LEETASAME+LTS V+QNADNARQA+ LA +AS+VAV+GG+ V QVV M I+ +S KI Sbjct: 298 LEETASAMEELTSTVKQNADNARQANSLAATASEVAVQGGSVVGQVVQTMGEINDASRKI 357 Query: 358 ADIITVIDGIAFQTNLLALNAAVEAARAGEHGRGFAVVASEVRGLAQRSAVAAKEIKSLI 417 DII+VIDGIAFQTN+LALNAAVEAARAGE GRGFAVVASEVR LAQRSA AAKEIK+LI Sbjct: 358 VDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKEIKALI 417 Query: 418 EGSVGHVKAGSNIVEQTVATIDEVIASIKHATDLITDISASSKEQSEGIEQINQAISQMD 477 + SV V GS +VEQ T+ EV+AS++ TD++ +ISA+S EQS+GIEQIN AI+QMD Sbjct: 418 DDSVSRVDNGSRLVEQAGTTMTEVVASVRRVTDVVAEISAASNEQSDGIEQINHAITQMD 477 Query: 478 QMTQENSALVEQNAAASQALQSQAEQLSALMGRFRLS 514 Q+TQ+N+ALVE+ AAA+Q+LQ Q+ +LSA + F+LS Sbjct: 478 QVTQQNAALVEEAAAAAQSLQEQSTRLSATVSVFKLS 514 >gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 583 Score = 381 bits (979), Expect = e-107, Method: Compositional matrix adjust. Identities = 243/530 (45%), Positives = 343/530 (64%), Gaps = 8/530 (1%) Query: 1 MKLANFNIGVRLGGAFAVVLLLIMVTAMVGVRNQSSNNAEINSIVNEKYQLIALSNQIKN 60 M+ N +I RL AF++++L+I +G+ + N ++ +VN++Y+ IA+ N I Sbjct: 1 MRFNNLSIATRLAIAFSILILVIAGIVALGLARLADINKALDLVVNDRYKKIAIINTISG 60 Query: 61 NGYKANAVLSNILLATTPEASAKYMDEYAGLRKANTEAYKQLEGMLDSDKTKALYAEQTT 120 L N LL T P A L NT+ Y +L ++ + + KA + T Sbjct: 61 KVDDVAISLRNQLLMTDPAQIAAEQKTIESLSAENTKLYDELTRLVANPEAKAKLDKVTE 120 Query: 121 ARSAYGVAVRKFFELMKTGERAEATTLFQGDLARLQTEYFVMVDKMVNSLAQEMVEDSRQ 180 R +Y ++ +L +G++ A L ++ LQ YF +D++ ++ Q++++ S Sbjct: 121 TRKSYAALRKQIDKLQSSGDKDAAIKLMLSEMVPLQKIYFSQLDELSDT-QQKLMDQSVD 179 Query: 181 VAEDGHLAVIQMIVLAAV-AVVLASVIGVLITRSITAPVRHAVVLAEAVAQGDLSYRLNA 239 AE + ++V+A V A A+ I I+RSIT P+ AV +AE VA GDL+ + A Sbjct: 180 QAEAAYETTRMIMVIAGVLAATFAAFIAWTISRSITRPLSRAVEVAETVAAGDLTAVIAA 239 Query: 240 AGKDEISRLLQALQHMSDNLHDTVSNVRNGTSAIDMAARELAQGNLSLSDRTEQQASSLE 299 DE RLLQAL M+ L V VR GT ++ A+ ++A GNL LS RTE+QAS+LE Sbjct: 240 HSNDETGRLLQALAAMNQKLKAIVLEVRRGTDSMVTASTQIATGNLDLSSRTEEQASALE 299 Query: 300 ETASAMEQLTSAVRQNADNARQASELAVSASDVAVRGGASVEQVVNIMTAISTSSGKIAD 359 ETAS++E+LTS+V+QNAD+ R++SELA +AS VA +GG +V+QVV M AI+ SS KI D Sbjct: 300 ETASSLEELTSSVKQNADHTRKSSELAANASQVANQGGLAVKQVVQTMGAINDSSHKIVD 359 Query: 360 IITVIDGIAFQTNLLALNAAVEAARAGEHGRGFAVVASEVRGLAQRSAVAAKEIKSLIEG 419 II+VIDGIAFQTN+LALNAAVEAARAGE GRGFAVVASEVRGLAQRSA AAKEIK LI Sbjct: 360 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRGLAQRSATAAKEIKELIND 419 Query: 420 SVGHVKAGSNIVEQTVATIDEVIASIKHATDLITDISASSKEQSEGIEQINQAISQMDQM 479 SV V +G+ +V Q +T+D V+ SI+ + ++ +ISA+++EQS+GIEQ+NQAI QMD Sbjct: 420 SVNQVTSGTELVAQAGSTMDAVVNSIEQVSHIVGEISAATREQSDGIEQVNQAIMQMDNT 479 Query: 480 TQENSALVEQNAAASQALQSQAEQLSALMGRFRLSELLANGQAGGHAASP 529 TQ+N+ALVE+ AAA+QALQ QA++L L+G FRL E+ GG +P Sbjct: 480 TQQNAALVEEAAAAAQALQDQAQRLLDLVGVFRLDEV------GGSGPAP 523 >gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 581 Score = 365 bits (938), Expect = e-102, Method: Compositional matrix adjust. Identities = 237/529 (44%), Positives = 333/529 (62%), Gaps = 31/529 (5%) Query: 3 LANFNIGVRLGGAFAVVLLLIMVTAMVGVRNQSSNNAEINSIVNEKYQLIALSNQIKNNG 62 N + VRL FA ++ L+ V ++ ++ N I+ IVN++Y + + +++ Sbjct: 5 FKNLKVAVRLAAVFAFIVALLAVVSVTAFIKINNINRAIDQIVNDRYLKVRWAFDLRDGV 64 Query: 63 YKANAVLSNILLATTPEASAKYMDEYAGLRKANTEAYKQLEGMLDSDKT--KALYAEQTT 120 + L I + R AN + + QLE + K + A Q T Sbjct: 65 NEQIKYLRGIAIDIEN-------------RPANEKRFAQLEVAVKQTKEALDKITARQVT 111 Query: 121 A------------RSAYGVAVRKFFELMKTGERAEATTLFQGDLARLQTEYFVMVDKMVN 168 A R A+ + F L+K G A + Q Y + + Sbjct: 112 AVGQKKAKALDEARIAFDASKADFLALVKAGNSTSADEYVLRKITDTQNNYLALARAFAD 171 Query: 169 SL-AQEMVEDSRQVAEDGHLAVIQMIVLAAVAVVLASVI-GVLITRSITAPVRHAVVLAE 226 S AQ E ++ VA DG +A I++ + ++A VLA++I G L+ RSI P+ AV +AE Sbjct: 172 SQDAQLQAEGAKAVA-DGAMA-IEVTLACSMAAVLAAIILGYLMARSIVKPLTEAVRVAE 229 Query: 227 AVAQGDLSYRLNAAGKDEISRLLQALQHMSDNLHDTVSNVRNGTSAIDMAARELAQGNLS 286 VA GDL+ R+ +DE +L+QAL+ M+DNL + VS VR GT +I A+ E+A GNL Sbjct: 230 NVAAGDLTTRIEPHSRDETGQLMQALRKMNDNLIEIVSGVRRGTESITTASGEIASGNLD 289 Query: 287 LSDRTEQQASSLEETASAMEQLTSAVRQNADNARQASELAVSASDVAVRGGASVEQVVNI 346 LS RTEQQA SLEETASAME++TS VRQNADNARQA+++AVSAS++A++GG V +VV+ Sbjct: 290 LSARTEQQAGSLEETASAMEEMTSTVRQNADNARQANQMAVSASEIALQGGQIVGRVVHT 349 Query: 347 MTAISTSSGKIADIITVIDGIAFQTNLLALNAAVEAARAGEHGRGFAVVASEVRGLAQRS 406 M I+ SS KI DII VIDGIAFQTN+LALNAAVEAARAGE GRGFAVVA+EVR LAQRS Sbjct: 350 MDEINQSSRKIVDIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRS 409 Query: 407 AVAAKEIKSLIEGSVGHVKAGSNIVEQTVATIDEVIASIKHATDLITDISASSKEQSEGI 466 A AAKEIK+LI SVG V+ GS +V Q AT+++V++S++ TD++ +ISA+S EQS GI Sbjct: 410 AAAAKEIKALIADSVGKVEGGSALVAQAGATMEQVVSSVRSVTDIVAEISAASAEQSSGI 469 Query: 467 EQINQAISQMDQMTQENSALVEQNAAASQALQSQAEQLSALMGRFRLSE 515 E+IN+A++QMD+ TQ+N+ALVEQ AAA++++Q QA +L+ + F+L + Sbjct: 470 EEINRAVTQMDEGTQQNAALVEQAAAAAKSMQEQAVRLTQSISIFKLEQ 518 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 364 bits (935), Expect = e-102, Method: Compositional matrix adjust. Identities = 232/518 (44%), Positives = 323/518 (62%), Gaps = 10/518 (1%) Query: 3 LANFNIGVRLGGAFAVVLLLIMVTAMVGVRNQSSNNAEINSI----VNEKYQLIALSNQI 58 + N IGVRL F +L+L+ V +GV S + + + ++ + I Sbjct: 1 MKNLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRIALKKERDAVQWHAAI 60 Query: 59 KNNGYKANAVLSNILLATTPEASAKYMDEYAGLRKAN-TEAYKQLEGMLDSDKTKALYAE 117 K NG + AV+ + +A Y + + A ++ K++E + + K L+ E Sbjct: 61 KENGVRTFAVMK-----SDDDAFQAYFQKQIDAQSARISQLQKEVEAAITDAEEKRLFDE 115 Query: 118 QTTARSAYGVAVRKFFELMKTGERAEATTLFQGDLARLQTEYFVMVDKMVNSLAQEMVED 177 R+AY + F++ G+ A A + L ++ +Y V + + Q + + Sbjct: 116 VGKLRAAYRATRQSIFDVKTAGDDARAREMTDTTLVKMMDDYADGVLRYADFQKQVIDQT 175 Query: 178 SRQVAEDGHLAVIQMIVLAAVAVVLASVIGVLITRSITAPVRHAVVLAEAVAQGDLSYRL 237 + + D ++ L A+ +VL + + L+TRSIT P+ AV +AE VA GDL+ R+ Sbjct: 176 AAAINRDYRSGRSMLLALGAIEIVLGAALAWLLTRSITRPLNQAVTIAETVAAGDLTSRI 235 Query: 238 NAAGKDEISRLLQALQHMSDNLHDTVSNVRNGTSAIDMAARELAQGNLSLSDRTEQQASS 297 +++ +DE +LL AL+ M+ NL D V VR GT I+ A+RE+A GNL LS RTE QA S Sbjct: 236 DSSSRDETGKLLAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDLSARTEAQAGS 295 Query: 298 LEETASAMEQLTSAVRQNADNARQASELAVSASDVAVRGGASVEQVVNIMTAISTSSGKI 357 LEETASAMEQLTS V+QNADNARQA+ELA SAS VA +GGA V+QVV M AI SS KI Sbjct: 296 LEETASAMEQLTSTVKQNADNARQANELAASASQVATQGGAVVDQVVATMGAIHASSQKI 355 Query: 358 ADIITVIDGIAFQTNLLALNAAVEAARAGEHGRGFAVVASEVRGLAQRSAVAAKEIKSLI 417 ADII VIDGIAFQTN+LALNAAVEAARAGE GRGFAVVASEVR LAQRSA AAKEIK LI Sbjct: 356 ADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQLI 415 Query: 418 EGSVGHVKAGSNIVEQTVATIDEVIASIKHATDLITDISASSKEQSEGIEQINQAISQMD 477 + SV V GS +V + T+ EV+ S+ T ++ +I+ +S+EQS+GIEQ+N AI+ +D Sbjct: 416 DDSVEKVDDGSRLVARAGDTMQEVVQSVARVTAVVREITLASQEQSDGIEQVNLAITHID 475 Query: 478 QMTQENSALVEQNAAASQALQSQAEQLSALMGRFRLSE 515 + TQ+N+ALVEQ AAA++++Q QA L+ + FRL + Sbjct: 476 ETTQQNAALVEQAAAAARSMQDQAASLTDAVSVFRLED 513 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.125 0.317 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,186,689 Number of extensions: 37544 Number of successful extensions: 590 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 290 Number of HSP's successfully gapped: 70 Length of query: 547 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 457 Effective length of database: 1,193,720 Effective search space: 545530040 Effective search space used: 545530040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)