BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0019.0146.p
         (860 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312273|ref|YP_003776365.1| 2-oxoglutarate dehydrogenase ox...   602   e-173

>gi|300312273|ref|YP_003776365.1| 2-oxoglutarate dehydrogenase
           oxidoreductase [Herbaspirillum seropedicae SmR1]
          Length = 954

 Score =  602 bits (1551), Expect = e-173,   Method: Compositional matrix adjust.
 Identities = 334/875 (38%), Positives = 492/875 (56%), Gaps = 47/875 (5%)

Query: 15  SDARQALAHARIQSFIEAYRHEGYRIADIDPLGTLSVPEIPALLPQHHDLKDEDL--ALG 72
           +D+        +   I  YR  G R A++DPL     P IP L P  +   D D+     
Sbjct: 86  ADSDMGRKRVAVTQLIAGYRDLGSRFANLDPLQRQERPNIPELDPSFYGFTDADMDIVFN 145

Query: 73  QHRFLEGLGLTNVKELTHFLSRLYCGPLGLDASGVRDESRRQWLFARLAARDPGADPATA 132
            +    G    ++++L   L   Y   +G + + + D +  +WL  RL         ++ 
Sbjct: 146 INNTYFGPETASLRDLLQMLRDTYTRSIGAEFTYISDMAEVRWLEERLEPTRAVPSFSSE 205

Query: 133 ERAAILARLAAAEQWEHYLQQDYPHAKRFSLEGCEALLVLMDALAERSSFYQLEQLFLAM 192
           ++  IL RL AAE  E YL   Y   KRFSLEG E+ +  +D   +R+    ++++ + M
Sbjct: 206 KKKHILERLTAAEGLERYLHTKYVGQKRFSLEGGESFIASLDETIQRAGEKGVQEIVIGM 265

Query: 193 PHRGRLNLLVNLMQMPAADIVAYFDAASTTAAAAGAVDLPYHLGAETVRATAHGQLGLFM 252
            HRGRLN+LVN++     ++ + F+        AG  D+ YH G  +  +T  G + L +
Sbjct: 266 AHRGRLNVLVNILGKTPQELFSEFEGRHADDLPAG--DVKYHQGFSSDVSTPGGPVHLSL 323

Query: 253 AHNPSHLESAYPVLLGMVRGY--RSAHPQSPMPASVVIHGDAAFCGQGVVMESLKLSLQP 310
           A NPSHLE   PV+ G V+    R           +++HGDAAF GQGVVME+L L+   
Sbjct: 324 AFNPSHLEIVNPVVEGSVKARMDRRGDKDGSQVLPILVHGDAAFAGQGVVMETLNLAQTR 383

Query: 311 GYSVGGTVHVIVNNQIGFTTPNPLDPLNHGYCTDIARIVGAPVLHVNADQPEAVLRAARI 370
           GY  GGTVH+++NNQIGFTT +P D  +  YC+D+ +++ APVLHVN D PEAV+ A +I
Sbjct: 384 GYGTGGTVHIVINNQIGFTTSDPRDSRSTLYCSDVVKMIEAPVLHVNGDDPEAVVFATQI 443

Query: 371 AFDYRMQFGADVVIDLVGYRRWGHAEQDTATVTQPLLHGFIDKHPPVTQLYA-----QAG 425
           A DYRM+F  D+V+D++ +R+ GH EQDT  +TQPL++  I +HP   +LYA     Q  
Sbjct: 444 AVDYRMKFKKDIVVDIICFRKLGHNEQDTPALTQPLMYKKIAQHPGTRKLYADKLVTQQT 503

Query: 426 WPGSDDGATLLAV----------SSQQVLASFRN----------SRPSAPAAQRRAPALA 465
            P +D G  L+            +   VL +F++          +R    AA+   P   
Sbjct: 504 IP-ADGGEELVKAYRAAMDAGKHAIDPVLTNFKSKYAVDWLPFLNRKWTDAAETAVP--- 559

Query: 466 VKPDAPDLLRLRQFVERMTRLPEGFAAHPAVTRLVAQWQDSVSSSDHACSWNFAENMAYA 525
                  +  L++   ++T +PEGF  H  V +++   + ++   +    W   E++AYA
Sbjct: 560 -------MAELKRLAAKITTVPEGFKVHSLVEKVLND-RAAMGRGEMNLDWGMGEHLAYA 611

Query: 526 SLLWEGQGLRISGMDVQRGTFMQRHAVWHAIDAAQGVQQAFWPLRQVAAPGTALEVVNSP 585
           SL+  G  +R++G D  RGTF  RHAV H  +  +     F PL+ VA   +   V++S 
Sbjct: 612 SLVSSGYAVRLTGQDAGRGTFTHRHAVLHDQNRERWDAGTFIPLQNVAENQSTFTVIDSV 671

Query: 586 LSEEAVVGFEYGFSVQAGPGAMVIWEAQFGDFVNGAQIMIDQFIASGRGKWGYPSALTVL 645
           LSEEAV+ FEYG+S  A P  + IWEAQFGDF NGAQ+++DQFI+SG  KWG  S L  +
Sbjct: 672 LSEEAVLAFEYGYST-AEPNTLTIWEAQFGDFANGAQVVMDQFISSGEVKWGRASGLVQM 730

Query: 646 LPHGYEGVGPEHSTGYLSRMLLLCAQENMRVAYPSSSAQWFHLLRQQALQGDR-PLVVFT 704
           LPHGYEG GPEHS+  + R L LCA  NM+V  P+++AQ FHLLR+Q ++  R PL++ T
Sbjct: 731 LPHGYEGQGPEHSSARIERYLQLCADNNMQVVQPTTAAQIFHLLRRQMIRLFRKPLIIMT 790

Query: 705 PKSVLHGEVRSQSPLSALLQGDFQPLVEDADAPPDA-QVQRVVLCSGKVCYDLADGRRKA 763
           PKS+L  +  + SPLS L +G FQ ++ + D   DA +V+R+V CSGKV YDL + R++ 
Sbjct: 791 PKSLLRNK-DAGSPLSDLAKGSFQTVIPEVDETIDAKKVKRIVACSGKVYYDLVNARKER 849

Query: 764 GDTRTVLLTVEQLHPFPEDRLGAVLRSYPQLEEVIWVQEEERKQGAWLCVRDAIEAALPL 823
           G T T ++ VEQL+PFP     A L+ +P   E++W Q+E + QGAWL ++  I   L  
Sbjct: 850 GQTDTAIIRVEQLYPFPHKAFAAALKQFPNFNELVWAQDEPQNQGAWLQIQHNIFENLSE 909

Query: 824 QVRLRAVTRPDTDAGPTASKARHYAQQAALVQSVF 858
             +L    RP + +       +HYAQQ AL+++ F
Sbjct: 910 GQKLAYAGRPASASPAVGYYDKHYAQQKALIETAF 944


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.321    0.134    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 2,839,482
Number of extensions: 123120
Number of successful extensions: 364
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 356
Number of HSP's successfully gapped: 1
Length of query: 860
Length of database: 1,619,870
Length adjustment: 93
Effective length of query: 767
Effective length of database: 1,179,515
Effective search space: 904688005
Effective search space used: 904688005
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 135 (56.6 bits)