BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0138.p (348 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311886|ref|YP_003775978.1| multi-sensor signal transductio... 687 0.0 gi|300310534|ref|YP_003774626.1| transmembrane sensor histidine ... 135 7e-33 gi|300311282|ref|YP_003775374.1| transmembrane sensor histidine ... 112 7e-26 gi|300312939|ref|YP_003777031.1| transmembrane nitrate/nitrite s... 90 3e-19 gi|300312076|ref|YP_003776168.1| signal transduction histidine k... 64 1e-11 >gi|300311886|ref|YP_003775978.1| multi-sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 687 bits (1774), Expect = 0.0, Method: Compositional matrix adjust. Identities = 331/348 (95%), Positives = 341/348 (97%) Query: 1 MQARFNAFMNAAPMPTWIKDAQLRYSYVNPAQSAFYGMTPHDMDGMTDAELMTTGGAEAS 60 MQARFNAFMNAAPMPTWIKDAQLRYSYVNPAQSAFYGMTP DMDG+TD ELM++G A AS Sbjct: 128 MQARFNAFMNAAPMPTWIKDAQLRYSYVNPAQSAFYGMTPGDMDGLTDLELMSSGAAAAS 187 Query: 61 REYDRKVLEEKCELHTQETVFDHNHNARILDVVRFPISGEGKGDMVAGLAIDVTEREQSR 120 +E DRKVLE+KCELHTQETVFDHNHNARILDVVRFPISGEGKGDMVAGLAIDVTEREQSR Sbjct: 188 QENDRKVLEQKCELHTQETVFDHNHNARILDVVRFPISGEGKGDMVAGLAIDVTEREQSR 247 Query: 121 RELELAIQRQQLLSSRVIEVQERERQHLARGLHDDVGQSLTALKINLETIKKTGSLEGPS 180 R+LELAIQRQQLLSSRVIEVQERERQHLARGLHDDVGQSLTALKINLETIKK GSLEGPS Sbjct: 248 RDLELAIQRQQLLSSRVIEVQERERQHLARGLHDDVGQSLTALKINLETIKKMGSLEGPS 307 Query: 181 LQNGIDIVTAVLAQVRSLSLDLRPPQLDNLGLIAALRSYVEQKSKLANVNGWFESSGHDE 240 LQNGIDIVTAVLAQVRSLSLDLRPPQLDNLGLIAALRSYVEQKSKLANVNGWFESSGHDE Sbjct: 308 LQNGIDIVTAVLAQVRSLSLDLRPPQLDNLGLIAALRSYVEQKSKLANVNGWFESSGHDE 367 Query: 241 ELHHDIENTAFRIVQEAVTNILRHAKCQNIWVKIDRQQDQVYLSIRDDGKGFDIERARSN 300 +LHHDIENTAFRIVQEAVTNILRHA+CQNIWVKIDRQ DQVYLSIRDDG+GFDIERARSN Sbjct: 368 QLHHDIENTAFRIVQEAVTNILRHARCQNIWVKIDRQHDQVYLSIRDDGRGFDIERARSN 427 Query: 301 AISGTSFGLLNMEERAVLVGGSMDITSAPGAGTEIVMHLPVAPIVRRM 348 AISGTSFGLLNMEERAVLVGGSM+ITSAPGAGTEIVMHLPVAPIVRRM Sbjct: 428 AISGTSFGLLNMEERAVLVGGSMEITSAPGAGTEIVMHLPVAPIVRRM 475 >gi|300310534|ref|YP_003774626.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 677 Score = 135 bits (339), Expect = 7e-33, Method: Compositional matrix adjust. Identities = 112/367 (30%), Positives = 178/367 (48%), Gaps = 41/367 (11%) Query: 3 ARFNAFMNAAPMPTWIKDAQLRYSYVNPAQSAFYGMTPHDMDGMTDAEL-MTTGGAEASR 61 A NA + P ++K+ Q + NPA G T + + EL + A+ Sbjct: 319 ALLNAVSESTSDPIFVKNRQGLMIFANPATLHKLGKTWEETMYRSSRELFLVPEEADTID 378 Query: 62 EYDRKVL----EEKCE--LHTQETVFDHNHNARILDVVRFPISG-EGKGDMVAGLAIDVT 114 DR+V+ EK E LH E V + P G +G V G++ D+T Sbjct: 379 RDDRRVMASGKPEKLEQTLHLPEGVVT-------FQTAKVPWFGKDGSVQGVIGISTDIT 431 Query: 115 EREQSRREL--------ELAIQRQQLL---SSRVIEVQERERQHLARGLHDDVGQSLTAL 163 ER+Q+ EL + IQR LL ++ + V+E E++ +AR LHD++G SLTAL Sbjct: 432 ERKQAEDELRQRESQLEKTVIQRTALLRELTNHLETVREEEKRAIARELHDNMGASLTAL 491 Query: 164 KINLETIKKTGSLEGPSLQNGID-------IVTAVLAQVRSLSLDLRPPQLDNLGLIAAL 216 ++LE + + P + D ++ +++A R + +LRP LD GL AA+ Sbjct: 492 SMHLEGVYQIL----PPDEKWADRKVRMQGLMKSLVATTRRIQTELRPNTLDLFGLKAAI 547 Query: 217 RSYVEQKSKLANVNGWFESSGHDEELHHDIENTAFRIVQEAVTNILRHAKCQNIWVKIDR 276 +++ + + D E+ H++E +R++QE + N+ +HAK + V +D Sbjct: 548 SEQLDELHERTGIACHASLPDEDVEVGHEMEIAIYRMLQEMLNNVTKHAKASKVDVILDI 607 Query: 277 QQDQVYLSIRDDGKGFDIERARSNAISGTSFGLLNMEERAVLVGGSMDITSAPGAGTEIV 336 +D V L++RDDG G ER R N ++GL + ERA GG +DI S PG G I Sbjct: 608 DEDHVALTVRDDGVGIPEER-RDNH---KTYGLRGLRERATFFGGEVDIRSTPGKGALIT 663 Query: 337 MHLPVAP 343 + LP+ P Sbjct: 664 ISLPIRP 670 >gi|300311282|ref|YP_003775374.1| transmembrane sensor histidine kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 464 Score = 112 bits (279), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 81/215 (37%), Positives = 117/215 (54%), Gaps = 15/215 (6%) Query: 133 LSSRVIEVQERERQHLARGLHDDVGQSLTALKINLET--IKKTGSLEGPS-----LQNGI 185 L+ RV+ QE ER L+R LHD + Q L ++K+ +E ++ G+ E + + Sbjct: 238 LAQRVVRSQEEERARLSRDLHDGISQWLVSIKLQIEAALVRLNGTPEQAATARAGFEKTA 297 Query: 186 DIVTAVLAQVRSLSLDLRPPQLDNLGLIAALRSYVEQ--KSKLANVNGWFESSGH--DEE 241 + VLA+VR +S DLRP LD+LGL AAL + + A V FE+ D Sbjct: 298 TQINGVLAEVRRISHDLRPAILDDLGLAAALEHLCGEWRDAAGAGVEIVFENQAQADDGA 357 Query: 242 LHHDIENTA-FRIVQEAVTNILRHAKCQNIWVKIDRQQDQVYLSIRDDGKGFDIERARSN 300 D+ NT FRI QEA+TNI RHA I V++ + + + L ++D+G GFD+ + + Sbjct: 358 TLPDVVNTVLFRIAQEALTNIARHAGASRIKVRLYQGHNLLMLQVQDNGHGFDLGQVDDH 417 Query: 301 AISGTSFGLLNMEERAVLVGGSMDITSAPGAGTEI 335 + G GL NM ER VGG + I S+P AGT I Sbjct: 418 SRHG--IGLRNMRERIEAVGGELGIDSSP-AGTSI 449 >gi|300312939|ref|YP_003777031.1| transmembrane nitrate/nitrite sensor histidine kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 650 Score = 90.1 bits (222), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 67/227 (29%), Positives = 117/227 (51%), Gaps = 15/227 (6%) Query: 121 RELELAIQRQQLLSSRVIEVQERERQHLARGLHDDVGQSLTALKINLETIKKTGSLE--- 177 R L AI+ Q+L++ ER LA+GLHD + Q L L + ++ ++ + + + Sbjct: 423 RHLGSAIENQRLIAREKEFAIANERNLLAQGLHDSIAQGLNFLNLQVQMLEDSLARDRLD 482 Query: 178 -----GPSLQNGIDIVTAVLAQVRSLSLDLRPPQLDNLGLIAALRSYVEQKSKLANVNGW 232 P L+ G V VR L L+ R +L + L + +R +++ + + + G Sbjct: 483 EIRDIAPLLRTG---VQENYEDVRELLLNFRT-RLQDSNLDSEMRKALDKLQRQSGLQGQ 538 Query: 233 FESSGHDEELHHDIENTAFRIVQEAVTNILRHAKCQNIWVKIDRQQDQVYLSIRDDGKGF 292 +G L + + I+QEA++NI +HA+ Q + + +D +QD +++ DDG GF Sbjct: 539 ITLTGDGPPLAPEQQLQVLFILQEALSNIRKHARAQQVQISVDNRQD-FRMTVSDDGHGF 597 Query: 293 DIERARSNAISGTSFGLLNMEERAVLVGGSMDITSAPGAGTEIVMHL 339 +E AR+ + GL M+ERA +G +++I S PG GT I + L Sbjct: 598 TLESARAKGLE--HVGLRIMQERAERLGANLEIDSQPGHGTRIALTL 642 >gi|300312076|ref|YP_003776168.1| signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 64.3 bits (155), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 56/211 (26%), Positives = 100/211 (47%), Gaps = 7/211 (3%) Query: 137 VIEVQERERQHLARGLHDDVGQSLTALKINLETIKKTGSLEG-----PSLQNGIDIVTAV 191 I ++E+ER+ +AR LHDD+G L + + L +++ + +G ++ Sbjct: 147 AIHIREQERERIARDLHDDLGGVLAGVGMALGRLQEQLAAQGLAAPLAEADEARQLLQQA 206 Query: 192 LAQVRSLSLDLRPPQLDNLGLIAALRSYVEQKSKLANVNGWFESSGHDEELHHDIENTAF 251 +A + S+ +L PP ++ GL+ AL+ Q + ++ + E+ + Sbjct: 207 MASMHSIIDELHPPIVE-FGLVDALQWQCRQIQREYRLHCQLDCPPTLEQEDRFLILGLL 265 Query: 252 RIVQEAVTNILRHAKCQNIWVKIDRQQDQVYLSIRDDGKGFDIERARSNAISGTSFGLLN 311 RI++EA +N RH + + V + +Q ++ + I DDG G A+ A GL N Sbjct: 266 RILREAASNAARHGRASALRVDLRMRQARLEMQIADDGCGIAPLHAKEVA-PRHGHGLRN 324 Query: 312 MEERAVLVGGSMDITSAPGAGTEIVMHLPVA 342 M ERA +GG + I + G I +PVA Sbjct: 325 MRERAQALGGELFIEAGQDGGVVIRTLIPVA 355 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,065,171 Number of extensions: 41833 Number of successful extensions: 172 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 162 Number of HSP's successfully gapped: 5 Length of query: 348 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 262 Effective length of database: 1,212,660 Effective search space: 317716920 Effective search space used: 317716920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)