BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0133.p (633 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311882|ref|YP_003775974.1| long-chain acyl-CoA synthetase ... 1207 0.0 gi|300313294|ref|YP_003777386.1| long chain acyl-CoA synthetase ... 92 1e-19 gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligas... 77 3e-15 gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligas... 77 3e-15 gi|300310928|ref|YP_003775020.1| medium-chain-fatty-acid CoA lig... 75 2e-14 >gi|300311882|ref|YP_003775974.1| long-chain acyl-CoA synthetase [Herbaspirillum seropedicae SmR1] Length = 653 Score = 1207 bits (3124), Expect = 0.0, Method: Compositional matrix adjust. Identities = 580/616 (94%), Positives = 596/616 (96%), Gaps = 2/616 (0%) Query: 20 WRRDKTRKXS--ASSFACGLAALGFKRGMSLAIIGNNCPRLYWAMSAAQALGGMPVPLYQ 77 W+ R+ + SFACGLAALGF+RGMSLAIIGNNCPRLYWAMSAAQALGGMPVPLYQ Sbjct: 38 WQTTSWRQVAEEVRSFACGLAALGFRRGMSLAIIGNNCPRLYWAMSAAQALGGMPVPLYQ 97 Query: 78 DAPAADMAYVLADANVDFVMAEDQEQVDKVFEIKENLPRIAHVIYDDERGMRNYHQPELL 137 DAPAADMAYVLADANVDFVMAEDQEQVDKVFEIKE+LPRIAHVIYDDERGMRNYHQPELL Sbjct: 98 DAPAADMAYVLADANVDFVMAEDQEQVDKVFEIKESLPRIAHVIYDDERGMRNYHQPELL 157 Query: 138 SFARVQELGRAYEHAHPGFFEQQVAAGSPDDVAIILYTSGTTGKPKGVCHSHRAMITTAT 197 SFARVQELGRAYE AHP FF+Q++AAGSPDDVAIILYTSGTTGKPKGVCHSHRAMITTAT Sbjct: 158 SFARVQELGRAYERAHPDFFDQEIAAGSPDDVAIILYTSGTTGKPKGVCHSHRAMITTAT 217 Query: 198 TLVEFDHLDENDDILCYLPLAWVGDFLYSFAQQHVAGFCLNCPESPNTVMTDLREIGPTY 257 TLVEFDHLDENDDILCYLPLAWVGDFLYSFAQQHVAGFCLNCPESPNTVMTDLREIGPTY Sbjct: 218 TLVEFDHLDENDDILCYLPLAWVGDFLYSFAQQHVAGFCLNCPESPNTVMTDLREIGPTY 277 Query: 258 YFAPPRVYENILTQVMIRIEDAGWIKRKMFHGFMKVARRVGMRILDGKPGVSLVDRLQYA 317 YFAPPRVYENILTQVMIRIEDAGW+KRKMFHGFMKVARRVGMRILDGKPGVSL+DRLQY Sbjct: 278 YFAPPRVYENILTQVMIRIEDAGWLKRKMFHGFMKVARRVGMRILDGKPGVSLLDRLQYG 337 Query: 318 IGDLLVYGPLKNVLGMSRLRVAYTGGEAIGPDLFDFYRSLGINLKQLYGMTETCVTVCMQ 377 +G LLVYGPLKNVLGMSRLRVAYTGGEAIGPDLFDFYRSLGINLKQLYGMTETCVTVCMQ Sbjct: 338 LGSLLVYGPLKNVLGMSRLRVAYTGGEAIGPDLFDFYRSLGINLKQLYGMTETCVTVCMQ 397 Query: 378 PSGDVKLDSVGRPMKGVEVRIDANGEVLVRSPGLMKEYFKRPDATAEAIDQDGYFHTGDA 437 PSGDVKLDSVGRPMKGVEVRID NGEVLVRSPGLMKEYFKRPDATAEAID +GYFHTGDA Sbjct: 398 PSGDVKLDSVGRPMKGVEVRIDENGEVLVRSPGLMKEYFKRPDATAEAIDANGYFHTGDA 457 Query: 438 GFFDSDGHLKIIDRAKDVGKMACGSMFAPKYIENKLKFFPFIKEAVTFGNGREQCMAFIN 497 GFFDSDGHLKIIDRAKDVGKMA GSMFAPKYIENKLKFFPFIKEAVTFGNGREQCMAFIN Sbjct: 458 GFFDSDGHLKIIDRAKDVGKMASGSMFAPKYIENKLKFFPFIKEAVTFGNGREQCMAFIN 517 Query: 498 IDMDAVGNWAERRNLPYSGYTDLAANPAVYDLVRECVEKVNADLVADPLLADSQIHRFLI 557 IDMDAVGNWAERRNL YSGYTDLAANPAVY L+RECVEKVNADL ADPLLADSQIHRFLI Sbjct: 518 IDMDAVGNWAERRNLAYSGYTDLAANPAVYALLRECVEKVNADLAADPLLADSQIHRFLI 577 Query: 558 LHKELDPDDEELTRTRKVRRGFIAEKYAVLIEALYAGHASQYIETQVKFEDGRQGMISAD 617 LHKELDPDDEELTRTRKVRRGFIA+KYAVLIEALYAG ASQYIETQVKFEDGRQGMISAD Sbjct: 578 LHKELDPDDEELTRTRKVRRGFIADKYAVLIEALYAGRASQYIETQVKFEDGRQGMISAD 637 Query: 618 LRIADAKTFKTLQSAA 633 L+IA+AKTFKTLQSAA Sbjct: 638 LKIAEAKTFKTLQSAA 653 >gi|300313294|ref|YP_003777386.1| long chain acyl-CoA synthetase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 92.4 bits (228), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 107/476 (22%), Positives = 184/476 (38%), Gaps = 92/476 (19%) Query: 158 EQQVAAGSPDDVAIILYTSGTTGKPKGVCHSHRAMITTATTLVE-FDHLDENDDILC-YL 215 E+ AA P+ + L+TSG+T PK V ++ R M + +V+ F L E IL +L Sbjct: 200 ERAFAATGPETIVKFLFTSGSTKMPKAVINTQRMMCSNLQMIVQTFPFLGEEPPILIDWL 259 Query: 216 PLAWVGDFLYSFAQQHVAGFCL------------NCPESPNTVMTDLREIGPTYYFAPPR 263 P + ++F H G L P+ T + +L +I PT YF P Sbjct: 260 P------WNHTFGANHNVGIVLYNGGTMYIDEGKPTPQGLATTLANLHDISPTVYFNVPV 313 Query: 264 VYENILTQVMIRIEDAGWIKRKMFHGFMKVARRVGMRILDGKPGVSLVDRLQYAIGDLLV 323 +E + +E ++ K + Sbjct: 314 GWEG----IAHALEKDQALREKFY------------------------------------ 333 Query: 324 YGPLKNVLGMSRLRVAYTGGEAIGPDLFDFYRSLG-------INLKQLYGMTETCVTVCM 376 SRLR+ + G A+ ++D + I + GMTET + Sbjct: 334 ----------SRLRMQFYAGAALAQPVWDKLHATAEATCGERIVMCCGLGMTETSPSALF 383 Query: 377 QPSGDVKLDSVGRPMKGVEVRIDANG---EVLVRSPGLMKEYFKRPDATAEAIDQDGYFH 433 +V+ +G P G+E+++ NG E+ R P + Y++ P+ TAEA D++GYF Sbjct: 384 VADLEVQAGQIGIPTPGMEIKLVPNGDKIEIRYRGPNVTPGYWRAPEQTAEAFDEEGYFR 443 Query: 434 TGDAGFFDSDGH----LKIIDRAKDVGKMACGSMFAPKYIENKLKF--FPFIKEAVTFGN 487 +GDA + H R + K+ G+ + + + P++++AV G Sbjct: 444 SGDAVRWLDPAHPDRGFMFDGRVAEDFKLYTGTWVSVGPLRALIAHEGAPYLQDAVITGQ 503 Query: 488 GREQCMAFINIDMDAVGNWAERRNLPYSGYTDLAANPAVYDLVRECVEKVNADLVADPLL 547 R++ I +++ A LP N A VR+ + V L + Sbjct: 504 DRKEIGMLIVPNIERCRQLA---RLPAEASRAEVLNAAP---VRDFFQGVLDRLQSHATG 557 Query: 548 ADSQIHRFLILHKELDPDDEELTRTRKVRRGFIAEKYAVLIEALYAGHASQYIETQ 603 + +++ R L+L D E+T + + + A L+E LYAG + Q Sbjct: 558 SSNRVTRALVLVDPPSFDRGEITDKGSINQRAVLTHRAALVETLYAGTDPAVLTVQ 613 >gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 566 Score = 77.4 bits (189), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 51/168 (30%), Positives = 78/168 (46%), Gaps = 13/168 (7%) Query: 331 LGMSRLRVAYTGGEAIGPDLFDFYRSL-GINLKQLYGMTETCVTVCMQPSG-DVKLDSVG 388 L S R GG A+ + D ++ L G + + YG++ET P D ++G Sbjct: 325 LDFSGYRCCVGGGMAVQKSVADKWKKLTGCPIIEGYGLSETSPIASANPCTIDEYTGTIG 384 Query: 389 RPMKGVE----------VRIDANGEVLVRSPGLMKEYFKRPDATAEAIDQDGYFHTGDAG 438 P+ E V + GE+ +R P +M Y++RPD TA+ + DG+F TGD G Sbjct: 385 LPLPSTEFAILDDDGQPVPLGQPGEIAIRGPQVMPGYWQRPDETAKVMTPDGFFKTGDIG 444 Query: 439 FFDSDGHLKIIDRAKDVGKMACGSMFAPKYIENKLKFFPFIKEAVTFG 486 D G+ KI+DR KD+ + G P +E + P + E G Sbjct: 445 VMDERGYTKIVDRKKDM-ILVSGFNVYPNEVEGVVAQHPGVLECACVG 491 >gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 77.4 bits (189), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 117/495 (23%), Positives = 178/495 (35%), Gaps = 106/495 (21%) Query: 20 WRRDKTRKXSASSFACGLAALGFKRGMSLAIIGNNCPRLYWAMSAAQALGGMPVPLYQDA 79 WR R + A L G K+G + + N +++ + AA +G + VP Sbjct: 65 WREINAR---VDALAHALTRRGVKKGDKMLVHSRNNQQIFESAWAAFKMGVVWVPTNFRI 121 Query: 80 PAADMAYVLADANVDFVMAEDQEQVDKVFEIKENLPRIAHVIYDDERGMRNYHQPELLSF 139 + AY L ++ VM D+ V +K P + HV+ L+ Sbjct: 122 TPPEAAY-LGQSSGACVMLYDRGFAQYVDAVKAASPALEHVV--------------ALAD 166 Query: 140 ARVQEL---GRAYEHAHPGFFEQQVAAGSPDDVAIILYTSGTTGKPKG--VCHSHRAMIT 194 AR EL E + F E +V DD YTSGTTG PK + H A + Sbjct: 167 ARAGELDYEALVREGSGAAFREAEV---DYDDPLWFFYTSGTTGHPKAGMLSHGQMAFVV 223 Query: 195 TATTLVEFDHLDENDDILCYLPLAWVGDFLYSFAQQHVAGF--CLNCPESPNTVMTDLRE 252 T L L PL+ H AG +N +++ Sbjct: 224 TNHLADLMPGLTHQSRSLVVAPLS------------HGAGVHAVINTARGAASIL----- 266 Query: 253 IGPTYYFAPPRVYENILTQVMIRIEDAGW--IKRKMFHGFMKVARRVGMRILDGKPGVSL 310 L+ + +E+A W +++ V V + + D Sbjct: 267 ----------------LSSERLVVEEA-WQLVEKYRVDNLFTVPTIVKILVEDES----- 304 Query: 311 VDRLQYAIGDLLVYGPLKNVLGMSRLRVAYTGGEAIGPDLFDFYRSLGINLKQLYGMTET 370 VDR ++ LK+V+ Y G D + LG L Q YG+ E Sbjct: 305 VDRYDHS--------SLKHVI--------YAGAPMYRADQVYALKKLGKVLVQYYGLGEV 348 Query: 371 CVTVCMQP---------SGDVKLDSVGRPMKGVEVRIDAN----------GEVLVRSPGL 411 + P ++ + G P G+++ I + GE+ VR P + Sbjct: 349 TGNITFLPPYLHFEDDAHPQARVGTCGMPRTGMQIAILNDDGQELAPFETGEICVRGPAV 408 Query: 412 MKEYFKRPDATAEAIDQDGYFHTGDAGFFDSDGHLKIIDRAKDVGKMACGSMFAPKYIEN 471 Y P+A A+A D +FHTGD G D DG L I R+ D+ ++ GS P+ IE Sbjct: 409 FMGYHNNPEANAKAFKHD-WFHTGDLGHVDQDGFLYITGRSSDM-YISGGSNVYPREIEE 466 Query: 472 KLKFFPFIKEAVTFG 486 L P + E G Sbjct: 467 ALLTHPAVSEVAVLG 481 >gi|300310928|ref|YP_003775020.1| medium-chain-fatty-acid CoA ligase [Herbaspirillum seropedicae SmR1] Length = 545 Score = 75.1 bits (183), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 55/186 (29%), Positives = 92/186 (49%), Gaps = 27/186 (14%) Query: 342 GGEAIGPDLFDFYRSLGINLKQLYGMTETC--VTVCMQPSGDVKLD-------SVGRPMK 392 GG A+ L R GI++ YGM+ETC +T+ P+ ++ + GRP+ Sbjct: 306 GGSAMTGSLAKMARDRGIDVFTGYGMSETCPVLTLAQVPTEELGGEREAAIRVKTGRPLP 365 Query: 393 GVEVRI------------DANGEVLVRSPGLMKEYFKRPDATAEAIDQDGYFHTGDAGFF 440 V++R GEV+VR+P L + Y + +A+ E + + G+ HT D G Sbjct: 366 LVDLRTVDGEMQDCPRDGSTTGEVVVRAPWLTQGYLHQAEASEE-LWRGGWLHTQDIGHL 424 Query: 441 DSDGHLKIIDRAKDVGKMACGSMFAPKYIENKLKFFPFIKEAVTFG--NGR--EQCMAFI 496 D++G+L++ DR KDV K G + IE+ + P + E G +G+ E+ +A + Sbjct: 425 DAEGYLQVTDRIKDVIKTG-GEWVSSLQIEDIIARHPAVAETAVIGIQDGKWGERPVALV 483 Query: 497 NIDMDA 502 + DA Sbjct: 484 VLKKDA 489 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.139 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,067,181 Number of extensions: 90236 Number of successful extensions: 277 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 270 Number of HSP's successfully gapped: 7 Length of query: 633 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 542 Effective length of database: 1,188,985 Effective search space: 644429870 Effective search space used: 644429870 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)