BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0130.p (358 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311879|ref|YP_003775971.1| amino acid ABC transporter perm... 701 0.0 gi|300310287|ref|YP_003774379.1| branched-chain amino acid ABC t... 333 1e-92 gi|300311115|ref|YP_003775207.1| branched-chain amino acid ABC t... 125 5e-30 gi|300310993|ref|YP_003775085.1| branched-chain amino acid ABC t... 116 3e-27 gi|300309520|ref|YP_003773612.1| branched-chain amino acid ABC t... 113 3e-26 >gi|300311879|ref|YP_003775971.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 358 Score = 701 bits (1810), Expect = 0.0, Method: Compositional matrix adjust. Identities = 354/358 (98%), Positives = 357/358 (99%) Query: 1 MLYREAGQFKTSYIADSQIFPIRQDRIIFTLFMVFAFVGVPLLGSEYWFSAILVPFLVFA 60 MLYREAGQFKTSYIADSQIFPIRQDRIIFTLFMVFAFVGVPLLGSEYWFSAILVPFLVFA Sbjct: 1 MLYREAGQFKTSYIADSQIFPIRQDRIIFTLFMVFAFVGVPLLGSEYWFSAILVPFLVFA 60 Query: 61 LAALGLNVLTGYAGQLSLGSAAFMAVGAYAAYNFQLRVEGIPIVATLLLSGACAAMVGVL 120 LAALGLNVLTGYAGQLSLGSAAFMAVGAYAAYNFQLRV+GIPIVATLLLSG CAA+VGVL Sbjct: 61 LAALGLNVLTGYAGQLSLGSAAFMAVGAYAAYNFQLRVDGIPIVATLLLSGGCAALVGVL 120 Query: 121 FGLPSLRIKGFYLAVATLAAQFFVVWALTKFPWFSNNSSSGVISTPKIDFFGIAIDTPVR 180 FGLPSLRIKGFYLAVATLAAQFFVVWALTKFPWFSNNSSSGVISTPKIDFFGIAIDTPVR Sbjct: 121 FGLPSLRIKGFYLAVATLAAQFFVVWALTKFPWFSNNSSSGVISTPKIDFFGIAIDTPVR 180 Query: 181 KYLFVLAVVSVLALVAKNLVRSSTGRAWMAVRDMDVAAEVIGIPLMRTKLTAFAVSSFYC 240 KYLFVLAVVSVLALVAKNLVRSSTGRAWMAVRDMDVAAEVIGIPLMRTKLTAFAVSSFYC Sbjct: 181 KYLFVLAVVSVLALVAKNLVRSSTGRAWMAVRDMDVAAEVIGIPLMRTKLTAFAVSSFYC 240 Query: 241 GVAGALYAFCYLGSVEPDGFSLDLSFRILFMIIIGGVGSILGSFLGSAFILLLPIFLDNA 300 GVAGALYAFCYLGSVEPDGFSLDLSFRILFMIIIGGVGSILGSFLGSAFILLLPIFLDNA Sbjct: 241 GVAGALYAFCYLGSVEPDGFSLDLSFRILFMIIIGGVGSILGSFLGSAFILLLPIFLDNA 300 Query: 301 LPPLASLLHLPFTNATVSHIQMMVFGALIIFFLIAEPHGLARLWQIAKEKLRLWPFPH 358 LPPLA+LLHLPFTNATVSHIQMMVFGALIIFFLIAEPHGLARLWQIAKEKLRLWPFPH Sbjct: 301 LPPLAALLHLPFTNATVSHIQMMVFGALIIFFLIAEPHGLARLWQIAKEKLRLWPFPH 358 >gi|300310287|ref|YP_003774379.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 347 Score = 333 bits (855), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 179/319 (56%), Positives = 235/319 (73%), Gaps = 5/319 (1%) Query: 37 FVGVPLLGSEYWFSAILVPFLVFALAALGLNVLTGYAGQLSLGSAAFMAVGAYAAYNFQL 96 +V VP + S Y A+L+PFL +LAA+GLN+LTGYAGQLSLGSAAFMAVGA+AAYN L Sbjct: 33 YVVVPWIASAYLIDALLIPFLALSLAAVGLNLLTGYAGQLSLGSAAFMAVGAFAAYNLNL 92 Query: 97 RVEGIPIVATLLLSGACAAMVGVLFGLPSLRIKGFYLAVATLAAQFFVVWALTKFPWFSN 156 RV G+P++ +++L+G A +G++FGLPSLR++GFYLAV+TLAAQFFV W L KF WFSN Sbjct: 93 RVAGLPLLVSIVLAGLAATAIGLVFGLPSLRLRGFYLAVSTLAAQFFVQWVLVKFSWFSN 152 Query: 157 NSSSGVISTPKIDFFGIAIDTPVRKYLFVLAVVSVLALVAKNLVRSSTGRAWMAVRDMDV 216 ++ SGVI P + G+A DTP +YLF LA+V+VL L+ LV+S TG ++AVRD + Sbjct: 153 DNPSGVIDAPPLVVGGLAFDTPAGRYLFALAIVTVLTLLCWRLVQSQTGHHFIAVRDNEQ 212 Query: 217 AAEVIGIPLMRTKLTAFAVSSFYCGVAGALYAFCYLGSVEPDGFSLDLSFRILFMIIIGG 276 AA VIG+P++RTKL AFA+SSF GVAG L+ F YL +VEP GF+LD S +ILF++IIGG Sbjct: 213 AARVIGVPVLRTKLLAFAISSFVIGVAGVLWGFVYLRTVEPAGFNLDRSLQILFIVIIGG 272 Query: 277 VGSILGSFLGSAFILLLPIFLDNALPPLASLLHLPFTNATVSHIQMMVFGALIIFFLIAE 336 + +I G+F G+A I++ P+ L + LL F + + Q +V GALIIFFLI E Sbjct: 273 LATIRGAFFGAALIVVFPLLLSRL---GSLLLGQWFDSGVLEMSQRIVLGALIIFFLIVE 329 Query: 337 PHGLARLWQIAKEKLRLWP 355 P GLA LWQ ++ RLWP Sbjct: 330 PQGLAALWQRGRQ--RLWP 346 >gi|300311115|ref|YP_003775207.1| branched-chain amino acid ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 655 Score = 125 bits (315), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 79/274 (28%), Positives = 155/274 (56%), Gaps = 7/274 (2%) Query: 27 IIFTLFMVFAFVGVPLLGSEYWFSAILVPFLVFALAALGLNVLTGYAGQLSLGSAAFMAV 86 ++ +L + A G+P++ ++ ++ L++A+ GL+++ GY GQ+SLG A + Sbjct: 6 LLLSLLGIVALAGIPMVVHNPYYLHLIETILIYAILLFGLDIVVGYTGQVSLGHAGLFGI 65 Query: 87 GAYAAYNFQLRVEGIPIVATLLLSGACAAMVGVLFGLPSLRIKGFYLAVATLAAQFFVVW 146 G+YAA ++ G+PI T+ + A A+ G + LP+LR+ G YLA+ TLA + Sbjct: 66 GSYAAGVLFFKL-GMPIFVTVPAAIAVTAVFGAILALPALRVTGPYLAMVTLAFGTIIQI 124 Query: 147 ALTKFPWFSNNSSSGVISTPKIDFFGIAIDTPVRKYLFVLAVVSVLA-LVAKNLVRSSTG 205 + + + + + P I ++ +++AV+ VL+ LV +++S+ G Sbjct: 125 LINEMTFLTEGPLGIKLQKPMIG----GDKMSEVQFFYLVAVLMVLSMLVVHRILKSNLG 180 Query: 206 RAWMAVRDMDVAAEVIGIPLMRTKLTAFAVSSFYCGVAGALYAFCYLGSVEPDGFSLDLS 265 RA+ A+RD +A++ +G+ + R K+ AF +S+ G++GALYA+ + P+ ++ +L+ Sbjct: 181 RAFQALRDSPIASDCMGVSVYRYKVYAFIISAALAGLSGALYAYSE-EYISPNTYNFELT 239 Query: 266 FRILFMIIIGGVGSILGSFLGSAFILLLPIFLDN 299 L +I+GG S +GS +G+ +++LP L + Sbjct: 240 IMFLLAVIMGGRKSRIGSLIGALIVVMLPSLLSD 273 >gi|300310993|ref|YP_003775085.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 408 Score = 116 bits (290), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 104/341 (30%), Positives = 165/341 (48%), Gaps = 45/341 (13%) Query: 23 RQDRIIFTLFMVFAFVGVPLLGSEYWFSAILVPFLVFALAALGLNVLTGYAGQLSLGSAA 82 RQ R + ++ A V P GS + + + L++ + LGLN++ G+AG L LG Sbjct: 78 RQQRAAVMILLMAALVW-PFFGSRGYVDVMTLA-LIYVVLGLGLNIVVGFAGLLDLGYVG 135 Query: 83 FMAVGAYAAYNFQLRVEGIPIVATLLLSGACAAMVGVLFGLPSLRIKGFYLAVATLAAQF 142 F AVGAY Y + G+ + L+ A +A+ G L G P LR++G YLA+ TL Sbjct: 136 FYAVGAYT-YALCNQYFGLTFWECVPLAAAASALFGFLLGFPVLRLRGDYLAIVTLGFGE 194 Query: 143 FVVWALTKFPWFSNNSSSGVISTPKIDFFGIAI-DTPVRK-------------------- 181 + L + GV PK FG+ + PV + Sbjct: 195 IIRLLLNNMTSITGGPD-GVSGIPKPTVFGLVMARNPVTEGGTTFHQLFGLSYQGGHMVI 253 Query: 182 YLFVLAVVSVL--ALVAKNLVRSSTGRAWMAVRDMDVAAEVIGIPLMRTKLTAFAVSSFY 239 +L+ LA++ VL V L+R GRAW A+R+ ++A +GI + KL+AF + + + Sbjct: 254 FLYFLALLMVLFTYFVTSRLLRMPMGRAWEALREDEIACRSLGINPTKVKLSAFTLGAAF 313 Query: 240 CGVAGALYAFCYLGSVEPDGFSLDLSFRILFMIIIGGVGSILGSFLGSAFILLLPIFLDN 299 G+AG+ +A G V P+ F+ S IL ++++GG+GS LG L A ILL Sbjct: 314 AGLAGSFFA-ARQGLVTPESFTFIESALILAIVVLGGMGSQLGVIL--AAILL------T 364 Query: 300 ALPPLASLLHLPFTNATVSHIQMMVFGALIIFFLIAEPHGL 340 LP LA + + +M++FG +++ ++ P GL Sbjct: 365 VLPELAR---------SFAEYRMLIFGLVMVLMMMWRPQGL 396 >gi|300309520|ref|YP_003773612.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 404 Score = 113 bits (282), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 99/348 (28%), Positives = 165/348 (47%), Gaps = 59/348 (16%) Query: 57 LVFALAALGLNVLTGYAGQLSLGSAAFMAVGAYAAY----------------------NF 94 L++ + ALGLNV+ G+AG L LG AF A+GAY+A NF Sbjct: 48 LLYIMLALGLNVVVGFAGLLDLGYIAFYAIGAYSAGLLASPQFAAVIESFVNTYPSVGNF 107 Query: 95 QLRVEGIPIVATLL---------LSGACAAMVGVLFGLPSLRIKGFYLAVATLAAQFFVV 145 + + G IV + +S AA+ G L G P+L+++G YLA+ TL + Sbjct: 108 LVWLCGPEIVQNGIHLSLWLIVPISAFLAALFGALLGAPTLKLRGDYLAIVTLGFGEIIR 167 Query: 146 WALTKF--PWFSNNSSSGVISTPKIDFFGIAI------------------DTPVRKYLFV 185 + P N G+ I FG+++ +LF+ Sbjct: 168 IFMNNLNAPVNITNGPQGINLIDPIKVFGVSLAGEPGSGSMVKVFGMSMPSVNAYYFLFL 227 Query: 186 LAVVSVLALVAKNLVRSSTGRAWMAVRDMDVAAEVIGIPLMRTKLTAFAVSSFYCGVAGA 245 L + V+ + L S GRAW+A+R+ ++AA+ +GI KL AFA+ + + GVAGA Sbjct: 228 LLCIGVIFFSVR-LQDSRLGRAWVAIREDEIAAKAMGINTRNVKLLAFAMGASFGGVAGA 286 Query: 246 LYAFCYLGSVEPDGFSLDLSFRILFMIIIGGVGSILGSFLGSAFILLLPIFLDNALPPLA 305 ++ + G V P+ FSL S +L M+++GG+G I G LG + LP L + + P+ Sbjct: 287 MFG-AFQGFVSPESFSLTESIAVLAMVVLGGIGHIPGVVLGGVILAALPEVLRHVVEPVQ 345 Query: 306 -SLLHLPFTNATVSHIQMMVFGALIIFFLIAEPHGLARLWQIAKEKLR 352 ++ + +A V ++ +++G ++ ++ P G LW + + R Sbjct: 346 MAIFGKVWIDAEV--LRQLLYGLAMVVIMLTRPAG---LWPSPRHEDR 388 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.331 0.144 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 851,528 Number of extensions: 27768 Number of successful extensions: 203 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 190 Number of HSP's successfully gapped: 19 Length of query: 358 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 272 Effective length of database: 1,212,660 Effective search space: 329843520 Effective search space used: 329843520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 131 (55.1 bits)