BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0119.p (261 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311869|ref|YP_003775961.1| short-chain alcohol dehydrogena... 417 e-118 gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herb... 122 3e-29 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 110 2e-25 gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herb... 108 3e-25 gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein... 106 2e-24 >gi|300311869|ref|YP_003775961.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 417 bits (1073), Expect = e-118, Method: Compositional matrix adjust. Identities = 208/261 (79%), Positives = 228/261 (87%), Gaps = 4/261 (1%) Query: 1 MNAATASSNTTPRIALITGGSRGLGKNAALHLAREGVGILLTYVGNRTAADATVSEIEAL 60 MN T S PRIALITGGSRGLGKN ALHLAR+G G++LTYVGN+ AA+ATV+E++AL Sbjct: 1 MNTPTQS----PRIALITGGSRGLGKNTALHLARDGAGVILTYVGNQQAAEATVAELQAL 56 Query: 61 GVPAVALQLDLADSASVPAFAQQVSDALYRVWQVRQFNYLVNNGGMGIYGPIAETSEADF 120 G A AL+LDLADSAS PAFAQ V +AL WQ F+YLVNNGGMG++ I ETSEA F Sbjct: 57 GAVAAALRLDLADSASFPAFAQAVREALKTHWQAEHFHYLVNNGGMGMHASIEETSEAVF 116 Query: 121 DTLYKVHLKGPFFLTQALLPLLADGGRIINISSGLARFTLPGYAAYAAMKGGVEVMTRYM 180 D L+KVHLKGPFFLTQALLPLLA GGRIINISSGLARF+LPGYAAYA+MKGGVEVMTRYM Sbjct: 117 DALFKVHLKGPFFLTQALLPLLAQGGRIINISSGLARFSLPGYAAYASMKGGVEVMTRYM 176 Query: 181 AKEFGARGIAVNTVAPGAIETDFGGGRVRDDAELNAFIAGQTALGRVGVPDDIGAAIASL 240 AKEFGARGI VNTVAPGAIETDFGGGRVRD+AELN+F+A QTALGR G+PDDIGAAIASL Sbjct: 177 AKEFGARGIVVNTVAPGAIETDFGGGRVRDNAELNSFVAHQTALGRAGLPDDIGAAIASL 236 Query: 241 LREDNGWITAQRVEVSGGMFI 261 LR DNGWI AQRVEVSGGMFI Sbjct: 237 LRPDNGWINAQRVEVSGGMFI 257 >gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 122 bits (306), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 84/259 (32%), Positives = 132/259 (50%), Gaps = 11/259 (4%) Query: 2 NAATASSNTTPRIALITGGSRGLGKNAALHLAREGVGILLTYVGNRTAADATVSEIEALG 61 AA ++S ++A + GGSRG+G LA EG + TYV + A A VS ++A G Sbjct: 7 QAAVSTSTLVSKVAFVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVSSVQAKG 66 Query: 62 VPAVALQLDLADSASVPAFAQQVSDALYRVWQVRQFNYLVNNGGMGIYGPIAETSEADFD 121 A+A+Q D AD+A++ + ++ R + LVNN G+ GP+ + D D Sbjct: 67 GRALAIQADSADAAALQQAIRLAAETFGR------LDILVNNAGVLAMGPLDQFKLEDLD 120 Query: 122 TLYKVHLKGPFFLTQALLPLLADGGRIINISSGLA-RFTLPGYAAYAAMKGGVEVMTRYM 180 V+++ F TQ + +GGR+I+I S A R G A YA K + +T+ + Sbjct: 121 RTLAVNVRSVFVATQEAARYMGEGGRVISIGSTNADRIPFAGGAVYAMSKSALVGLTKGL 180 Query: 181 AKEFGARGIAVNTVAPGAIETDFGGGRVRDDAELNAFIAGQTALGRVGVPDDIGAAIASL 240 A++ G RGI VN V PG ++TD + A + G AL R G ++I + +A L Sbjct: 181 ARDLGPRGITVNNVQPGPVDTDMNPA----SGDFAASLTGLMALPRYGKAEEIASFVAYL 236 Query: 241 LREDNGWITAQRVEVSGGM 259 ++G+IT + + GG Sbjct: 237 AGPESGYITGANLMIDGGF 255 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 110 bits (274), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 86/259 (33%), Positives = 136/259 (52%), Gaps = 13/259 (5%) Query: 6 ASSNTTPRIALITGGSRGLGKNAALHLAREGVGILLTYVGNRTAADATVSEIEALGVPA- 64 ++ N ++AL+TG SRG+G+ A LAR+G ++ T + + A A + ALG A Sbjct: 2 SAQNLQGQVALVTGASRGIGRAIATELARQGAKVIGTAT-SESGAAAITEYLAALGPEAG 60 Query: 65 VALQLDLADSASVPAFAQQVSDALYRVWQVRQFNYLVNNGGMGIYGPIAETSEADFDTLY 124 L++ D+ + A +QV + LVNN G+ + ++D++ Sbjct: 61 RGAVLNVNDAEASVALVEQVQKDFG------SLSILVNNAGITQDQLAMRMKDEEWDSVI 114 Query: 125 KVHLKGPFFLTQALLP--LLADGGRIINISSGLARFTLPGYAAYAAMKGGVEVMTRYMAK 182 +L L++A+L + A GRIINI+S + PG YAA K GV M+R +A+ Sbjct: 115 ATNLTAVGRLSRAVLRGMMKARTGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAR 174 Query: 183 EFGARGIAVNTVAPGAIETDFGGGRVRDDAELNAFIAGQTALGRVGVPDDIGAAIASLLR 242 E G+R I VN VAPG I+TD R ++ + +A I Q LGR+G ++I A+A L Sbjct: 175 EIGSRNITVNCVAPGFIDTDM--TRALNEQQ-SAAILQQIPLGRLGAAEEIAYAVAFLAS 231 Query: 243 EDNGWITAQRVEVSGGMFI 261 +IT + V+GGM++ Sbjct: 232 PQAAYITGTTLNVNGGMYM 250 >gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 369 Score = 108 bits (271), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 77/250 (30%), Positives = 117/250 (46%), Gaps = 9/250 (3%) Query: 13 RIALITGGSRGLGKNAALHLAREGVGILLTYVGNRTAADATVSEIEALGVPAVALQLDLA 72 ++ALITGG G+G+ AL AREG + + Y+ A T +++AL P + L D+ Sbjct: 126 KVALITGGDSGIGRAVALAYAREGARLAIVYLDEEEDARQTQKDVQALDAPCLLLPGDVG 185 Query: 73 DSASVPAFAQQVSDALYRVWQVRQFNYLVNNGGMGIYG-PIAETSEADFDTLYKVHLKGP 131 + + V DA R+ + LVNN I E S+A + ++ ++ Sbjct: 186 SPEFCRSAVRAVMDAYGRL------DVLVNNAAQQYPAQSIEEISQAQLEHTFRTNIFSM 239 Query: 132 FFLTQALLPLLADGGRIINISSGLARFTLPGYAAYAAMKGGVEVMTRYMAKEFGARGIAV 191 F++ + +P L G RIIN +S A YAA KG + TR ++++ + GI V Sbjct: 240 FYMVKEAMPFLTRGARIINTTSVTAYRGSKHLVDYAATKGAIVAFTRSLSQQLASAGIHV 299 Query: 192 NTVAPGAIETDFGGGRVRDDAELNAFIAGQTALGRVGVPDDIGAAIASLLREDNGWITAQ 251 N VAPG I T AE A LGR G PD++ A L E ++T Q Sbjct: 300 NAVAPGPIWTPLIPASF--SAEEVASFGKNVPLGRPGQPDEVAPAYVFLATEGASYMTGQ 357 Query: 252 RVEVSGGMFI 261 + +GG + Sbjct: 358 VLHPNGGEIV 367 >gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 245 Score = 106 bits (264), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 84/247 (34%), Positives = 124/247 (50%), Gaps = 15/247 (6%) Query: 16 LITGGSRGLGKNAALHLAREGVGILLTYVGNRTAADATVSEIEALGVPAVALQLDLADSA 75 +TG SRG+G+ AL LAREG I+L R AAD +EI ALG LQ D+A+ Sbjct: 10 FVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQVRVLQFDVANRE 69 Query: 76 SVPAFAQQVSDALYRVWQVRQFNYLVNNGGMGIYGPIAETSEADFDTLYKVHLKGPF-FL 134 A QV L V Q + +V N G+ D+D++ + +L G + L Sbjct: 70 Q----AAQV--LLADVEQHGCYYGVVCNAGITRDNAFPAMPGEDWDSVLRTNLDGFYNVL 123 Query: 135 TQALLPLLAD--GGRIINISSGLARFTLPGYAAYAAMKGGVEVMTRYMAKEFGARGIAVN 192 ++P++ GRI+ ++S G Y+A K GV T+ +A E R I VN Sbjct: 124 NPLVMPMVRRRAAGRIVTLASVSGLIGNRGQVNYSAAKAGVIGATKALALELAKRDITVN 183 Query: 193 TVAPGAIETDFGGGRVRDDAELNAFIAGQTALGRVGVPDDIGAAIASLLREDNGWITAQR 252 VAPG I+TD +DA ++ + A R G P+++ AA+A L+ D +IT Q Sbjct: 184 CVAPGLIDTDMT-----EDAPIDEILKLIPAR-RAGKPEEVAAAVAFLMSPDAAYITRQV 237 Query: 253 VEVSGGM 259 + V+GG+ Sbjct: 238 ISVNGGL 244 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 911,983 Number of extensions: 41719 Number of successful extensions: 288 Number of sequences better than 1.0e-08: 47 Number of HSP's gapped: 217 Number of HSP's successfully gapped: 47 Length of query: 261 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 178 Effective length of database: 1,226,865 Effective search space: 218381970 Effective search space used: 218381970 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)