BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0115.p (248 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313993|ref|YP_003778085.1| diguanylate cyclase/phosphodies... 131 6e-32 gi|300310067|ref|YP_003774159.1| diguanylate cyclase GGDEF famil... 118 4e-28 gi|300309626|ref|YP_003773718.1| diguanylate cyclase [Herbaspiri... 117 7e-28 gi|300312843|ref|YP_003776935.1| response regulator containing a... 117 1e-27 gi|300310865|ref|YP_003774957.1| response regulator containing a... 115 4e-27 >gi|300313993|ref|YP_003778085.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 573 Score = 131 bits (329), Expect = 6e-32, Method: Compositional matrix adjust. Identities = 61/107 (57%), Positives = 79/107 (73%) Query: 142 KELEALSYRDGLTNVANRRMFDSALEVEWANAVNTRAPLSMIMLDIDYFKQYNDHYGHIR 201 ++LEALS DGLT VANRR FD AL E AV + L+++MLD+DYFK+YND YGH+ Sbjct: 396 RKLEALSATDGLTGVANRRRFDEALANEMRRAVRSGQSLALLMLDVDYFKKYNDRYGHLA 455 Query: 202 GDECLKQVAQLLSQSATRSRDFFARYGGEEFVLILPDCDEKAALQVA 248 GDECL+ VA++L +S+ R+ D ARYGGEEF +I+ +CD ALQ A Sbjct: 456 GDECLRIVARVLQKSSRRATDLVARYGGEEFSVIIAECDTTQALQQA 502 >gi|300310067|ref|YP_003774159.1| diguanylate cyclase GGDEF family protein [Herbaspirillum seropedicae SmR1] Length = 369 Score = 118 bits (296), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 58/118 (49%), Positives = 74/118 (62%), Gaps = 3/118 (2%) Query: 127 ITERKKTEARLVELQKELEALSYRDGLTNVANRRMFDSALEVEWANAVNTRAPLSMIMLD 186 I ER + L + ++L +LS DGLT +ANRR FD LE EW ++ PL + +D Sbjct: 179 IAERTRD---LAQANEQLRSLSLTDGLTGIANRRSFDETLEQEWRRSMRHGTPLMLAFID 235 Query: 187 IDYFKQYNDHYGHIRGDECLKQVAQLLSQSATRSRDFFARYGGEEFVLILPDCDEKAA 244 +D+FK YNDHYGH+ GD L+QVAQ L QS R D ARYGGEEF +I D D +A Sbjct: 236 VDWFKPYNDHYGHLAGDAVLRQVAQTLRQSIARVGDTVARYGGEEFAIIAADTDGQAG 293 >gi|300309626|ref|YP_003773718.1| diguanylate cyclase [Herbaspirillum seropedicae SmR1] Length = 358 Score = 117 bits (294), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 57/107 (53%), Positives = 72/107 (67%), Gaps = 1/107 (0%) Query: 142 KELEALSYRDGLTNVANRRMFDSALEVEWANAVNTRAPLSMIMLDIDYFKQYNDHYGHIR 201 ++LE + D LT + NRR FD L EW + PLS++M+D+DYFKQYND YGH Sbjct: 196 QKLEQQTLTDALTGIGNRRSFDLRLREEWTRHLRNARPLSLLMVDVDYFKQYNDTYGHPA 255 Query: 202 GDECLKQVAQLLSQSATRSRDFFARYGGEEFVLILPDCDEKAALQVA 248 GD L Q+ ++L +S R+ DF ARYGG+EF LILPD DE ALQVA Sbjct: 256 GDALLTQLGRVL-RSPLRASDFLARYGGDEFSLILPDTDEIGALQVA 301 >gi|300312843|ref|YP_003776935.1| response regulator containing a CheY-like receiver and a GGDEF domains protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 117 bits (292), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 54/105 (51%), Positives = 73/105 (69%) Query: 144 LEALSYRDGLTNVANRRMFDSALEVEWANAVNTRAPLSMIMLDIDYFKQYNDHYGHIRGD 203 LE L+++DGLT +ANRR D+ LE E+ A + A L++IM+D+D+FKQYND YGH GD Sbjct: 343 LEMLAHQDGLTGLANRRHLDTMLETEFKRAERSGAALALIMIDVDFFKQYNDLYGHQAGD 402 Query: 204 ECLKQVAQLLSQSATRSRDFFARYGGEEFVLILPDCDEKAALQVA 248 ECL++VA +L + R D ARYGGEE ++LP D + A VA Sbjct: 403 ECLRRVAGVLKERQRRPGDLAARYGGEEMAMLLPGTDAEGACAVA 447 >gi|300310865|ref|YP_003774957.1| response regulator containing a CheY-like receiver domain and a GGDEF domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 554 Score = 115 bits (288), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 68/161 (42%), Positives = 97/161 (60%), Gaps = 18/161 (11%) Query: 100 HIWIRDVVHVVRNPDGSPNALIGF-----MFDITERKKTEARLVELQKELEAL------- 147 H+W+R + V+ A+I F + + R++ EA+ + ++ELE+L Sbjct: 299 HVWVRGIGVVL------LLAVICFGGKRLVVLVAGRERAEAQAIAARQELESLYRTLEAQ 352 Query: 148 SYRDGLTNVANRRMFDSALEVEWANAVNTRAPLSMIMLDIDYFKQYNDHYGHIRGDECLK 207 S +DGLT+V NRR FD+AL E A T LS++M+D+D+FKQ+ND YGH GD CL+ Sbjct: 353 SQKDGLTDVFNRRYFDAALAAELARLNRTGESLSLMMIDVDHFKQFNDTYGHAAGDVCLR 412 Query: 208 QVAQLLSQSATRSRDFFARYGGEEFVLILPDCDEKAALQVA 248 QVA +S A R D ARYGGEEF +ILP+C +A +A Sbjct: 413 QVAAAISTVARRQGDIVARYGGEEFAVILPNCHPGSAQGLA 453 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 754,553 Number of extensions: 29006 Number of successful extensions: 181 Number of sequences better than 1.0e-08: 38 Number of HSP's gapped: 146 Number of HSP's successfully gapped: 38 Length of query: 248 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 165 Effective length of database: 1,226,865 Effective search space: 202432725 Effective search space used: 202432725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)