BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0104.p (264 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311859|ref|YP_003775951.1| CobW/P47K family protein [Herba... 495 e-141 gi|300310263|ref|YP_003774355.1| cobalamin synthesis P47K protei... 271 4e-74 gi|300314043|ref|YP_003778135.1| cobalamin synthesis protein/P47... 130 1e-31 gi|300311960|ref|YP_003776052.1| G3E family GTPase [Herbaspirill... 85 6e-18 >gi|300311859|ref|YP_003775951.1| CobW/P47K family protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 495 bits (1274), Expect = e-141, Method: Compositional matrix adjust. Identities = 242/259 (93%), Positives = 254/259 (98%) Query: 1 VTVLSGFLGAGKTTLLNHILHNREGRRVAVIVNDMSEVNIDAALVRDGGADLSRTEEKLV 60 VTVLSGFLGAGKTTLLNHILHNREGRRVAVIVNDMSEVNIDAALVRDGGADL+RTEEKLV Sbjct: 15 VTVLSGFLGAGKTTLLNHILHNREGRRVAVIVNDMSEVNIDAALVRDGGADLARTEEKLV 74 Query: 61 EMSNGCICCTLREDLLMEVRKLARERRFDQLVIESTGISEPLPVAETFTFTDEEGASLAD 120 EMSNGCICCTLREDLLMEVRKLARERRFDQLVIESTGISEPLPVAETFTFTDE+GASL+D Sbjct: 75 EMSNGCICCTLREDLLMEVRKLARERRFDQLVIESTGISEPLPVAETFTFTDEDGASLSD 134 Query: 121 VARLDTMVTVVDAFNFLKDYSSQDSLQERGESMGEEDSRTVVDLLIEQIEFCDVIVLNKL 180 VARLDTMVTVVDAFNFLKDYSSQDSL +RG+SMGEED+RTVVDLLIEQIEFCDVIVLNKL Sbjct: 135 VARLDTMVTVVDAFNFLKDYSSQDSLDQRGQSMGEEDNRTVVDLLIEQIEFCDVIVLNKL 194 Query: 181 DLVSPLDLERLHAILHKLNPRARIVTSEFGKVALDQVLNTGLFDFEQASQAPGWLQELRG 240 DLV+ DLERLHAILHKLNPRARIV +EFGKVAL+QVL+TGLFDFEQASQAP WLQELRG Sbjct: 195 DLVAADDLERLHAILHKLNPRARIVRAEFGKVALEQVLDTGLFDFEQASQAPSWLQELRG 254 Query: 241 EHVPETEQYGITSFAWRAR 259 EHVPETEQYGI+SFAW+AR Sbjct: 255 EHVPETEQYGISSFAWQAR 273 >gi|300310263|ref|YP_003774355.1| cobalamin synthesis P47K protein [Herbaspirillum seropedicae SmR1] Length = 413 Score = 271 bits (693), Expect = 4e-74, Method: Compositional matrix adjust. Identities = 145/261 (55%), Positives = 186/261 (71%), Gaps = 8/261 (3%) Query: 1 VTVLSGFLGAGKTTLLNHILHNREGRRVAVIVNDMSEVNIDAALVRDGGADLSRTEEKLV 60 VTVLSGFLG+GKTTLLNHIL NR G +VAVIVNDMSEVN+D VR +L R ++LV Sbjct: 19 VTVLSGFLGSGKTTLLNHILRNRAGMKVAVIVNDMSEVNMDGDDVRRN-TELYRGNDELV 77 Query: 61 EMSNGCICCTLREDLLMEVRKLARERRFDQLVIESTGISEPLPVAETFTFTDEEGASLAD 120 EMSNGCICCTLR DLL +V LAR RFD L+IESTGISEP+PVAETF F D +G SL++ Sbjct: 78 EMSNGCICCTLRADLLEQVSALARSGRFDYLLIESTGISEPIPVAETFAFLDSDGFSLSE 137 Query: 121 VARLDTMVTVVDAFNFLKDYSSQDSL-QERGESMGEEDSRTVVDLLIEQIEFCDVIVLNK 179 +ARLDT+VTVV+ F + + ++ Q G S +R + DLLIEQ+E+ +VI++++ Sbjct: 138 LARLDTLVTVVNGETFEQQLADHVAIAQADGAS-----TRPLSDLLIEQVEYANVILVSR 192 Query: 180 LDLVSPLDLERLHAILHKLNPRARIVTSEFGKVALDQVLNTGLFDFEQASQAPGWLQELR 239 +DL+ L A+L KLNP ARI+ G+V LDQVL+T LFD ++ PGW+Q + Sbjct: 193 VDLIDASAFASLKAVLQKLNPTARILPMAHGEVDLDQVLDTRLFDLPSLARMPGWMQTMD 252 Query: 240 GEHVP-ETEQYGITSFAWRAR 259 G H P E + YGI+S +RAR Sbjct: 253 GAHPPSEADTYGISSSVYRAR 273 >gi|300314043|ref|YP_003778135.1| cobalamin synthesis protein/P47K family protein [Herbaspirillum seropedicae SmR1] Length = 388 Score = 130 bits (327), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 86/244 (35%), Positives = 131/244 (53%), Gaps = 36/244 (14%) Query: 1 VTVLSGFLGAGKTTLLNHILHNREGRRVAVIVNDMSEVNIDAALVRDGGADLSRTEEKLV 60 V++L+GFLG+GKTTLLNH++ E + VI+N+ E+ +D LV EE +V Sbjct: 14 VSLLTGFLGSGKTTLLNHLITQPEMKDTLVIINEFGEIGLDHLLVAHS------QEETIV 67 Query: 61 EMSNGCICCTLREDLLMEVRKL------ARERRFDQLVIESTGISEPLPVAETFTFTDEE 114 EMS+GC+CCT+R DL ++ + R+F+++VIE+TG++ P P+ T TD Sbjct: 68 EMSSGCLCCTIRGDLKKTLKDITWRFAEGGRRKFNRVVIETTGLASPAPILHTL-MTD-- 124 Query: 115 GASLADVARLDTMVTVVDAFNFLKDYSSQDSLQERGESMGEEDSRTVVDLLIEQIEFCDV 174 A +A+ RLD ++ VDA N + S+ D +E ++Q D Sbjct: 125 -AFIAERYRLDGVIVTVDAVNGM---STLDHHEE----------------AVQQAAVADR 164 Query: 175 IVLNKLDLVSPLDLERLHAILHKLNPRARIVTSEFGKVALDQVLNTGLFDF-EQASQAPG 233 ++L K DLV L L A L LNP AR + + G++A ++L+ GLF E+ Sbjct: 165 LLLTKTDLVDAATLAALQARLRTLNPGARQLQPQVGQIAAAELLDAGLFKPGEKMPDVAR 224 Query: 234 WLQE 237 WL E Sbjct: 225 WLNE 228 >gi|300311960|ref|YP_003776052.1| G3E family GTPase [Herbaspirillum seropedicae SmR1] Length = 377 Score = 85.1 bits (209), Expect = 6e-18, Method: Compositional matrix adjust. Identities = 70/224 (31%), Positives = 114/224 (50%), Gaps = 21/224 (9%) Query: 1 VTVLSGFLGAGKTTLLNHILHNREGRRVAVIVNDMSEVNIDAALVRDGGADLSRTEEKLV 60 VTV++GFLGAGKTTLL +L R+ RR+A+++N+ EV ID L+R G ++ + ++ Sbjct: 19 VTVVTGFLGAGKTTLLRGLLQRRQSRRLALLINEFGEVAIDGDLLRAEGD--AQGQVQIQ 76 Query: 61 EMSNGCICCTLREDLL-MEVRKLARERRFDQLVIESTGISEPLPVAETFTFTDEEGASLA 119 + ++G I + + AR + D ++IE++G++ P V E +GA +A Sbjct: 77 DFAHGLIAYGDDAHFVPAMLALAARRAQIDHVLIETSGLALPSAVMEVL-----QGAEMA 131 Query: 120 DVARLDTMVTVVDAFNFLKD-YSSQDSLQERGESMGEEDSRTVVDLLIEQIEFCDVIVLN 178 LD + VVD L+D ++S D++ L +Q+E DV+VLN Sbjct: 132 RHFVLDATLAVVDTPLLLEDAFASADAV-----------GAATAQLFRQQLECADVVVLN 180 Query: 179 KLDLVSPLDLERLHAILHKLNPRARIVTSEFGKVALDQVLNTGL 222 K+D + L + + L P R + +G LD L GL Sbjct: 181 KIDGMEEAPLLQAEQAVRALAPNVRFLELAYG-ARLDIRLALGL 223 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 772,898 Number of extensions: 30749 Number of successful extensions: 166 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 157 Number of HSP's successfully gapped: 4 Length of query: 264 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 181 Effective length of database: 1,226,865 Effective search space: 222062565 Effective search space used: 222062565 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)