BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0019.0098.p
         (342 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311855|ref|YP_003775947.1| sphingosine kinase/eukaryotic d...   542   e-155

>gi|300311855|ref|YP_003775947.1| sphingosine kinase/eukaryotic
           diacylglycerol kinase [Herbaspirillum seropedicae SmR1]
          Length = 339

 Score =  542 bits (1396), Expect = e-155,   Method: Compositional matrix adjust.
 Identities = 277/344 (80%), Positives = 297/344 (86%), Gaps = 7/344 (2%)

Query: 1   MTEQDTTQELPASHGHPDAARRPDVVAAINARAGGGHAEELAAHITEEFARHGLHAVVHL 60
           M +Q+TT  LP     P +  RPDVVA INA+AGGGHAEELAA I+ EFARHG HA V L
Sbjct: 1   MPDQNTTSPLP-----PHSQARPDVVAVINAKAGGGHAEELAARISAEFARHGRHARVRL 55

Query: 61  ARSGEDMLQAARNAVRDKIAVVAVGGGDGSVNAVASTLLADPHCESALGVLPLGTLNHFA 120
           A SGEDML  AR AVRD++ VVAVGGGDGSVNAVASTLLAD  C+SALGVLPLGTLNHFA
Sbjct: 56  AASGEDMLATARQAVRDRVPVVAVGGGDGSVNAVASTLLADAQCQSALGVLPLGTLNHFA 115

Query: 121 KDLNIPLTLDEAIANIATGRKLRVDSGEVNGAPFINNSSLGLYPDIVREREKQQARLGRG 180
           KDLNIPLTL+ AIANIATGR+LRVDS EVNG  FINNSSLGLYPDIVREREKQQARLGRG
Sbjct: 116 KDLNIPLTLEGAIANIATGRRLRVDSAEVNGVAFINNSSLGLYPDIVREREKQQARLGRG 175

Query: 181 KWLAFTWAAMGALRRYPFLRVRLSIDGKEHWRRTPFVFIGNNEYLMSGLDIGKRSTLTDG 240
           KWLAFTWAAMGALRRYPFLRVRLSIDG+EHWRRTPFVFIGNN YLMSGLDIGKRS+LT+G
Sbjct: 176 KWLAFTWAAMGALRRYPFLRVRLSIDGQEHWRRTPFVFIGNNPYLMSGLDIGKRSSLTEG 235

Query: 241 QLSLYVCHRTGRLGLLRLALHALFGRLREAHDFDVLTCSEIHIETHKKRMRVATDGEVNL 300
            LSLYVCH TGR GLLRLALHALFGRLR+AHDFD+LT ++IHIETHK+RMRVATDGEV L
Sbjct: 236 CLSLYVCHHTGRWGLLRLALHALFGRLRQAHDFDMLTATDIHIETHKRRMRVATDGEVQL 295

Query: 301 MQTPLHYRIRPASLTVIVPQVADPQAPSTSEP--GLLDKLLGEN 342
           MQTPL YRIRPASL VIVPQ     A + S P  GLLDKL GE+
Sbjct: 296 MQTPLSYRIRPASLEVIVPQAPASAATTASPPTGGLLDKLWGES 339


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.320    0.136    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 961,199
Number of extensions: 34831
Number of successful extensions: 111
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 111
Number of HSP's successfully gapped: 1
Length of query: 342
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 256
Effective length of database: 1,212,660
Effective search space: 310440960
Effective search space used: 310440960
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 131 (55.1 bits)