BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0064.p (340 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 649 0.0 gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 522 e-149 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 224 9e-60 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 211 6e-56 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 204 1e-53 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 649 bits (1673), Expect = 0.0, Method: Compositional matrix adjust. Identities = 320/341 (93%), Positives = 332/341 (97%), Gaps = 1/341 (0%) Query: 1 VTLALMALGTMLIAFVPSYATIGVLAPVLVLVGRLLQGFSAGVELGGVSVYLAEMATPGN 60 VTLALMALGTMLIAFVPSYATIG LAP+LVL+GRLLQGFSAGVELGGVSVYLAEMATPGN Sbjct: 88 VTLALMALGTMLIAFVPSYATIGALAPLLVLIGRLLQGFSAGVELGGVSVYLAEMATPGN 147 Query: 61 KGFYVSWQSASQQVAII-XAAIGYMLSTTLTPAQVGDWGWRVPFFIGCLIVPVLFVIRRS 119 KGFYVSWQSASQQVAII AAIGY+LST+LTPA+V WGWR+PFFIGCLIVPVLFVIRRS Sbjct: 148 KGFYVSWQSASQQVAIIVAAAIGYLLSTSLTPAEVSGWGWRIPFFIGCLIVPVLFVIRRS 207 Query: 120 LQETEEFMRRKHRPSTGQIFRSMIENWKLVIAGMMLVSMTTVSFYLITVYTPTFGKNVLK 179 LQETEEFMRRKHRPSTGQIFRSMIENWKLVIAGMMLVSMTTVSFYLITVYTPTFGKNVLK Sbjct: 208 LQETEEFMRRKHRPSTGQIFRSMIENWKLVIAGMMLVSMTTVSFYLITVYTPTFGKNVLK 267 Query: 180 LSTEASLIVTLCVGLSNFIWLPIMGALSDRIGRRPILVTFTVLTILTAYPAVNWLVHDAS 239 LSTEASLIVTLCVGLSNFIWLPIMGALSDR+GRRP+LVTFTVLTILTAYPAVNWLVHDAS Sbjct: 268 LSTEASLIVTLCVGLSNFIWLPIMGALSDRVGRRPLLVTFTVLTILTAYPAVNWLVHDAS 327 Query: 240 FAKMLMVELWLSFLYASYNGAMVVALTEVMPAEVRTVGFSLAYSLATAIFGGFTPAIATG 299 FAKML+VELWLSFLYASYNGAMVVALTEVMPA+VRT GFSLAYSLATA+FGGFTPAIATG Sbjct: 328 FAKMLIVELWLSFLYASYNGAMVVALTEVMPADVRTAGFSLAYSLATAVFGGFTPAIATG 387 Query: 300 LIEMTHDKAAPGLWMSTAAVCGLIATLILYRNKAARQAVTA 340 LIE THDKAAPG+WMSTAAVCGLIATLILYRNKA RQA TA Sbjct: 388 LIEYTHDKAAPGIWMSTAAVCGLIATLILYRNKAVRQAATA 428 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 522 bits (1345), Expect = e-149, Method: Compositional matrix adjust. Identities = 260/340 (76%), Positives = 298/340 (87%), Gaps = 1/340 (0%) Query: 1 VTLALMALGTMLIAFVPSYATIGVLAPVLVLVGRLLQGFSAGVELGGVSVYLAEMATPGN 60 VTLA+MA+GT+LIAFVP YATIGV AP+LVL GRLLQGFSAGVELGGVSVYLAEMATPGN Sbjct: 90 VTLAIMAIGTLLIAFVPGYATIGVAAPILVLTGRLLQGFSAGVELGGVSVYLAEMATPGN 149 Query: 61 KGFYVSWQSASQQVAIIXAA-IGYMLSTTLTPAQVGDWGWRVPFFIGCLIVPVLFVIRRS 119 KGFYVSWQSASQQ AI+ +A IGY++S +L+P Q+ DWGWR+PFFIGC+IVP +F+IRRS Sbjct: 150 KGFYVSWQSASQQAAIMASALIGYLVSQSLSPVQISDWGWRIPFFIGCMIVPAIFIIRRS 209 Query: 120 LQETEEFMRRKHRPSTGQIFRSMIENWKLVIAGMMLVSMTTVSFYLITVYTPTFGKNVLK 179 LQET+EF+ RK PS G+I S+ +NWK+V+AGMM+V MTTVSFYLITVYTPTFGKNVLK Sbjct: 210 LQETDEFLARKRHPSFGEIMSSIAQNWKIVVAGMMMVVMTTVSFYLITVYTPTFGKNVLK 269 Query: 180 LSTEASLIVTLCVGLSNFIWLPIMGALSDRIGRRPILVTFTVLTILTAYPAVNWLVHDAS 239 LS SLIVT CVGLSNF WLPIMGALSDR+GRRP+LV FT+LT+LTAYP ++WLV D S Sbjct: 270 LSASDSLIVTFCVGLSNFCWLPIMGALSDRVGRRPVLVAFTLLTLLTAYPVMSWLVSDIS 329 Query: 240 FAKMLMVELWLSFLYASYNGAMVVALTEVMPAEVRTVGFSLAYSLATAIFGGFTPAIATG 299 F +L+ ELWLSFLYASYNGA VVALTEVMPA VRTVGFSLAYSLATAIFGGFTP ++T Sbjct: 330 FKNLLITELWLSFLYASYNGAAVVALTEVMPAHVRTVGFSLAYSLATAIFGGFTPLVSTW 389 Query: 300 LIEMTHDKAAPGLWMSTAAVCGLIATLILYRNKAARQAVT 339 LIE + DKAAPG WMS AAVCGL+ATL+LYR K+A V+ Sbjct: 390 LIETSGDKAAPGYWMSFAAVCGLLATLVLYRGKSAAATVS 429 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 224 bits (570), Expect = 9e-60, Method: Compositional matrix adjust. Identities = 133/328 (40%), Positives = 191/328 (58%), Gaps = 6/328 (1%) Query: 1 VTLALMALGTMLIAFVPSYATIGVLAPVLVLVGRLLQGFSAGVELGGVSVYLAEMATPGN 60 +++ LM +GT +IAF+PSYA+IG+LAP + +G+++QGFSAG E G + +L E A P Sbjct: 95 LSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMIQGFSAGGEFGSSTAFLVEHA-PHR 153 Query: 61 KGFYVSWQSASQQVAIIXAAI-GYMLSTTLTPAQVGDWGWRVPFFIGCLIVPVLFVIRRS 119 +GF+ SWQ ASQ ++++ AA+ G +L+ LTP Q+ WGWRVPF G LI P IRR Sbjct: 154 RGFFSSWQVASQGISLLLAALFGAVLNNMLTPEQLASWGWRVPFIFGLLIAPAGIYIRRH 213 Query: 120 LQETEEFMRRKHRPSTGQIFRSMIENWKL-VIAGMMLVSMTTVSFYLITVYTPTFGKNVL 178 L E EF + T R + K+ ++ G V M TVS YL ++Y PT+ L Sbjct: 214 LDEAPEFKESSEK--TNAPLRDTFAHQKMRLLIGAGSVIMATVSVYL-SLYIPTYAVKQL 270 Query: 179 KLSTEASLIVTLCVGLSNFIWLPIMGALSDRIGRRPILVTFTVLTILTAYPAVNWLVHDA 238 L +S GL FI P++GALSD+IGR P ++T + L I+ YP +L + Sbjct: 271 GLPAWSSFAAMSVAGLIMFIGSPLVGALSDKIGRTPFMITSSALYIVLTYPMFVFLTNSP 330 Query: 239 SFAKMLMVELWLSFLYASYNGAMVVALTEVMPAEVRTVGFSLAYSLATAIFGGFTPAIAT 298 F ++L+++ + L Y AM L ++ P R G SLAY++A IFGGF I T Sbjct: 331 GFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVATRGTGMSLAYNIAVTIFGGFAGLIIT 390 Query: 299 GLIEMTHDKAAPGLWMSTAAVCGLIATL 326 LI+ T DK + ++ AV LIA+L Sbjct: 391 WLIDFTGDKLSVSYFVIFGAVLSLIASL 418 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 211 bits (537), Expect = 6e-56, Method: Compositional matrix adjust. Identities = 124/327 (37%), Positives = 187/327 (57%), Gaps = 4/327 (1%) Query: 1 VTLALMALGTMLIAFVPSYATIGVLAPVLVLVGRLLQGFSAGVELGGVSVYLAEMATPGN 60 VT+ LMA+GT +I P+Y +IG+ AP++++V RLLQGFSAG E+G + +L E A Sbjct: 97 VTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLLQGFSAGGEMGSATAFLTEYAPERE 156 Query: 61 KGFYVSWQSASQQVAIIX-AAIGYMLSTTLTPAQVGDWGWRVPFFIGCLIVPVLFVIRRS 119 + +Y SW AS VA++ AA+G ++ +L A + WGWR+PF +G +I PV + IR Sbjct: 157 RAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALASWGWRLPFLLGIIIGPVGYYIRHH 216 Query: 120 LQETEEFMRRKHRPSTGQIFRSMIENW-KLVIAGMMLVSMTTVSFYLITVYTPTFGKNVL 178 L+ET F+ K + T + +I N+ + A +V + TV Y++ Y PT+ VL Sbjct: 217 LEETPTFLEEKDK--TDSPLKEVIRNFPRETSASFSMVILWTVCTYVLLFYMPTYSVKVL 274 Query: 179 KLSTEASLIVTLCVGLSNFIWLPIMGALSDRIGRRPILVTFTVLTILTAYPAVNWLVHDA 238 KL I + GL + PI+G L+DRIGRRP+L L ++ AYP ++ Sbjct: 275 KLPQTTGFIAGMVGGLCIMVLAPIVGRLADRIGRRPLLSGSAALILMLAYPMFAYINSAP 334 Query: 239 SFAKMLMVELWLSFLYASYNGAMVVALTEVMPAEVRTVGFSLAYSLATAIFGGFTPAIAT 298 S + +L+ +L L A+Y G ++ A +E+ PA+V + G S+AY+LA IFGGF T Sbjct: 335 SLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKVLSTGLSVAYNLAVTIFGGFASFFIT 394 Query: 299 GLIEMTHDKAAPGLWMSTAAVCGLIAT 325 LI T AP ++ AA + T Sbjct: 395 WLIAATGSTMAPAFYVMIAAAISFVGT 421 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 204 bits (518), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 117/328 (35%), Positives = 187/328 (57%), Gaps = 5/328 (1%) Query: 1 VTLALMALGTMLIAFVPSYATIGVLAPVLVLVGRLLQGFSAGVELGGVSVYLAEMATPGN 60 V + LM L +IAF P+YATIG+ AP++++V R+LQGF+ G E + +L E A Sbjct: 85 VIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGFATGGEYSSSTAFLVESAPAHK 144 Query: 61 KGFYVSWQSASQQVAIIXAA-IGYMLSTTLTPAQVGDWGWRVPFFIGCLIVPVLFVIRRS 119 +G Y SWQ Q +A+ A +G +++ L+P + WGWR+PF +G LI PV IRR Sbjct: 145 RGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDSWGWRLPFVLGLLIGPVGLWIRRH 204 Query: 120 LQETEEFMRRKHRPSTG--QIFRSMIENWKLVIAGMMLVSMTTVSFYLITVYTPTFGKNV 177 + ET++F+ ++P+ ++ + N + V+ M V TV+FY++ V PTF Sbjct: 205 MHETDDFLESANKPNAAPIKLLDVVKTNLRGVLVSMGQVINGTVAFYVVLVNMPTFANKQ 264 Query: 178 LKLSTEASLIVTL-CVGLSNFIWLPIMGALSDRIGRRPILVTFTVLTILTAYPAVNWLVH 236 L L + + +V + V L + +P GALSDR+GRR +L+ ++ +L YP W+ Sbjct: 265 LGLPLDQAFMVQMIAVALLTAV-IPFAGALSDRLGRRTVLLYSSLAFLLMVYPLFVWVSA 323 Query: 237 DASFAKMLMVELWLSFLYASYNGAMVVALTEVMPAEVRTVGFSLAYSLATAIFGGFTPAI 296 S ++L++++ L + + G M AL+E P VR+ G ++AY+LA +FGGF P I Sbjct: 324 APSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPTRVRSTGLAVAYNLAAMLFGGFAPFI 383 Query: 297 ATGLIEMTHDKAAPGLWMSTAAVCGLIA 324 T L + + AP ++ AA G+ A Sbjct: 384 VTWLTKTSGSPVAPAWYVLFAACIGVSA 411 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.137 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 951,379 Number of extensions: 35398 Number of successful extensions: 251 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 224 Number of HSP's successfully gapped: 13 Length of query: 340 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 254 Effective length of database: 1,212,660 Effective search space: 308015640 Effective search space used: 308015640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)