BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0053.p (812 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311818|ref|YP_003775910.1| 1,4-alpha-glucan branching prot... 1437 0.0 gi|300311642|ref|YP_003775734.1| 1,4-alpha-glucan branching prot... 993 0.0 gi|300311816|ref|YP_003775908.1| malto-oligosyltrehalose trehalo... 118 1e-27 gi|300311817|ref|YP_003775909.1| glycogen debranching enzyme pro... 62 1e-10 >gi|300311818|ref|YP_003775910.1| 1,4-alpha-glucan branching protein [Herbaspirillum seropedicae SmR1] Length = 804 Score = 1437 bits (3720), Expect = 0.0, Method: Compositional matrix adjust. Identities = 721/812 (88%), Positives = 749/812 (92%), Gaps = 8/812 (0%) Query: 1 MSGMSVTAYRASTTPQEETSDASSAAPGSASAQPPSSSHSSHPPEFPEQTALSLSLEEQG 60 MSGMSV AYRAS E +SDAS A P SAS S+ S HP E TALSLS EEQG Sbjct: 1 MSGMSVMAYRASVESPEASSDASFAPPSSAS-----SADSIHPLE---TTALSLSFEEQG 52 Query: 61 LLEALLAGRLTQPFGLLGPRYASQSSGVSAIRAFHPGAAAVRVRCRHSGALLGELQQFEL 120 +LEALLAGRLTQPF LLGPRYAS +A+R FHPGA AVRVRCRHSG LL ELQQ+ + Sbjct: 53 MLEALLAGRLTQPFDLLGPRYASAKGDGAAVRCFHPGAEAVRVRCRHSGVLLAELQQYPV 112 Query: 121 HGQPSGFFSGVATGLDQRRHQVPYVLEVDWLIPDGSRSVQSTEDPYAFGLLLGELDLHLL 180 GQPSGFFSG+ GL+QRRH+VPYVLEVDW IPDGSRSVQSTEDPYAFGLLLGELDLHLL Sbjct: 113 DGQPSGFFSGLVPGLEQRRHEVPYVLEVDWRIPDGSRSVQSTEDPYAFGLLLGELDLHLL 172 Query: 181 REGRHRQLADCLGARPMDIDGVPGTRFAVWAPNAERVSVVGDFNQWDGRRHVMRLRHESG 240 +EGRHRQLADCLGARPM+IDGVPGTRFAVWAPNAERVSVVGDFNQWDGRRHVMRLRHE G Sbjct: 173 QEGRHRQLADCLGARPMEIDGVPGTRFAVWAPNAERVSVVGDFNQWDGRRHVMRLRHEPG 232 Query: 241 VWELFIPRIGPGDVYKYEILGRDGALLPLKADPVARATEPPPATASVVADARPYLWQDQQ 300 VWELFIPRIGPGDVYKYEI GR G LLPLKADPVARATEPPPATASVVADARPY+WQDQQ Sbjct: 233 VWELFIPRIGPGDVYKYEIRGRGGVLLPLKADPVARATEPPPATASVVADARPYVWQDQQ 292 Query: 301 WMAERAQRQSLSAPMSIYELHPASWRRIPEQAHRNLSWDELADELIPYVVGMGFTHIELM 360 WMAERAQRQSLSAPMSIYE+HPASWRR+PEQ HRNL+WDELAD+LIPYVVGMGFTHIELM Sbjct: 293 WMAERAQRQSLSAPMSIYEVHPASWRRVPEQDHRNLNWDELADQLIPYVVGMGFTHIELM 352 Query: 361 PVMEHPFGGSWGYQPISMFAPSARFGKPERFAAFVDRCHQAGIGVLLDWVPAHFPSDQHG 420 PVMEHPFGGSWGYQPISMFAPSARFGKPERFAAFVDRCHQAGIGVLLDWVPAHFPSDQHG Sbjct: 353 PVMEHPFGGSWGYQPISMFAPSARFGKPERFAAFVDRCHQAGIGVLLDWVPAHFPSDQHG 412 Query: 421 LAQFDGTALYEHADPREGFHQDWNTLIFNLGRNEVRGFLIASALEWIEHFHVDGLRVDAV 480 LAQFDGTALYEHADPREGFHQDWNTLIFNLGRNEVRGFLIASALEW+EHFHVDGLRVDAV Sbjct: 413 LAQFDGTALYEHADPREGFHQDWNTLIFNLGRNEVRGFLIASALEWLEHFHVDGLRVDAV 472 Query: 481 ASMLYRDYSRKSGEWVPNIHGGRENLEAVAFLRELNTVVAQRCPGVLMIAEESTSWPGVT 540 ASMLYRDYSRK GEWVPNIHGGRENLEAVAFLRELNTVVAQRCPG LMIAEESTSWPGVT Sbjct: 473 ASMLYRDYSRKEGEWVPNIHGGRENLEAVAFLRELNTVVAQRCPGALMIAEESTSWPGVT 532 Query: 541 SEVSNEADHHGLGFSYKWNMGWMNDTLRYMQHDPLFRRYHHHDMTFGLLYGFSENFILPI 600 + V E DHHGLGFSYKWNMGWMNDTLRYMQHDPLFRRYHHHDMTFGLLYGFSENFILPI Sbjct: 533 APVKEEGDHHGLGFSYKWNMGWMNDTLRYMQHDPLFRRYHHHDMTFGLLYGFSENFILPI 592 Query: 601 SHDEVVHGKRALLNKMPGDEWRRFANVRAYYGFMWTHPGKKLLFMGSEFGQPDEFDHDKS 660 SHDEVVHGKRALLNKMPGD+WRRFANVRAY+GFMWTHPGKKLLFMGSEFGQPDEFDHDKS Sbjct: 593 SHDEVVHGKRALLNKMPGDQWRRFANVRAYFGFMWTHPGKKLLFMGSEFGQPDEFDHDKS 652 Query: 661 PYWHLLNEHGDHPAGHRQLRQLVSDLNHLYRSEPALHQRDCRADGFSWAVGDDSVNSVLA 720 PYWHLL+EH HPA HRQLRQLV DLN LY SEPALHQRDCRADGF+WAVGDDSVNSVLA Sbjct: 653 PYWHLLDEHAGHPAEHRQLRQLVGDLNRLYCSEPALHQRDCRADGFAWAVGDDSVNSVLA 712 Query: 721 YFRYADEGPPVLIVVNLTPVPRTHYRIGVPPIAPGHHGVWREVLNTDAAVYGGSNSGNMG 780 YFRYADEGPPVL+VVNLTPVPR +YRIGVP IAPGHHGVWREVLNTDA +YGGSN+GN+G Sbjct: 713 YFRYADEGPPVLVVVNLTPVPRVNYRIGVPSIAPGHHGVWREVLNTDAGLYGGSNNGNLG 772 Query: 781 QVQVQEFGWHGHPTSIELNLPPLSTLILRRES 812 VQVQE WHGH SIEL LPPLSTLILRRES Sbjct: 773 AVQVQELAWHGHAASIELTLPPLSTLILRRES 804 >gi|300311642|ref|YP_003775734.1| 1,4-alpha-glucan branching protein [Herbaspirillum seropedicae SmR1] Length = 819 Score = 993 bits (2566), Expect = 0.0, Method: Compositional matrix adjust. Identities = 503/792 (63%), Positives = 588/792 (74%), Gaps = 50/792 (6%) Query: 58 EQGLLEALLAGRLTQPFGLLGPRY------ASQSSGVSAIR--AFHPGAAAVRVRCRHS- 108 ++ L+ L+ GRL PF LLGPR A+Q + +R + PGA+AV+V R S Sbjct: 38 DESLVARLMQGRLGDPFALLGPREIADAPEATQKAKAKRLRLCCYFPGASAVQVYARTSA 97 Query: 109 -----------GALLGELQQF-------------ELHGQPSGFFSGVATGLDQRRHQ--V 142 G+LLG L ++ E Q SG F G L +R Q + Sbjct: 98 APDELHAPDSTGSLLGSLHEWSPATGQGESDHDQEAPRQRSGVFFG-EVKLPRRAVQPPI 156 Query: 143 PYVLEVDWLIPDGSRSVQSTEDPYAFGLLLGELDLHLLREGRHRQLADCLGARPMDIDGV 202 PYV V W DG ++Q TED YAFGLLL + DLHL REGRHRQLA CLG++ M IDGV Sbjct: 157 PYVFHVTWRGADGRETLQITEDAYAFGLLLSDFDLHLFREGRHRQLARCLGSQVMQIDGV 216 Query: 203 PGTRFAVWAPNAERVSVVGDFNQWDGRRHVMRLRHESGVWELFIPRIGPGDVYKYEILGR 262 G RFAVWAPNA+RVSV+GDFNQWDGRR+ MRLR +GVWELF+PR+G G +YKYEI+G Sbjct: 217 EGVRFAVWAPNAQRVSVIGDFNQWDGRRNPMRLRQSAGVWELFVPRLGDGALYKYEIIGP 276 Query: 263 DGALLPLKADPVARATEPPPATASVVADARPYL----WQDQQWMAERAQRQSLSAPMSIY 318 G LLP KADP+ARATE PPATAS V D + WQD W+ +RAQ Q+ APMSIY Sbjct: 277 HGELLPGKADPMARATEVPPATASRVIDDSKLVSGRRWQDAHWLQQRAQGQTRQAPMSIY 336 Query: 319 ELHPASWRRIPEQAHRNLSWDELADELIPYVVGMGFTHIELMPVMEHPFGGSWGYQPISM 378 E+H ASW R+P + +RNL W+ELAD LIPYV GMGFTH+EL+PVMEHPFGGSWGYQP+S Sbjct: 337 EVHAASWLRLPGEGNRNLDWNELADRLIPYVKGMGFTHVELLPVMEHPFGGSWGYQPLSQ 396 Query: 379 FAPSARFGKPERFAAFVDRCHQAGIGVLLDWVPAHFPSDQHGLAQFDGTALYEHADPREG 438 FAPSARFG P FA FVD CH+AGIGVLLDWVPAHFPSD HGLA FDGT LYEH DPREG Sbjct: 397 FAPSARFGPPAEFATFVDACHRAGIGVLLDWVPAHFPSDPHGLAYFDGTPLYEHGDPREG 456 Query: 439 FHQDWNTLIFNLGRNEVRGFLIASALEWIEHFHVDGLRVDAVASMLYRDYSRKSGEWVPN 498 HQDWNT I+NLGR+EVRGFLIASALEW+EHFHVDGLRVDAVASMLYRDYSR SG+WVPN Sbjct: 457 IHQDWNTHIYNLGRHEVRGFLIASALEWLEHFHVDGLRVDAVASMLYRDYSRSSGQWVPN 516 Query: 499 IHGGRENLEAVAFLRELNTVVAQRCPGVLMIAEESTSWPGVTSEVSNEADHHGLGFSYKW 558 IHGGRENLEAV FLRELN VA+RCPG LMIAEEST+WPGVT+ VS GLGF+YKW Sbjct: 517 IHGGRENLEAVDFLRELNQTVAERCPGALMIAEESTAWPGVTAPVSEG----GLGFTYKW 572 Query: 559 NMGWMNDTLRYMQHDPLFRRYHHHDMTFGLLYGFSENFILPISHDEVVHGKRALLNKMPG 618 NMGWM+DTLRY++ D ++RRYHHHD+TFG++Y FSE+F+LPISHDEVVHGK LL KM G Sbjct: 573 NMGWMHDTLRYVELDSIYRRYHHHDITFGMIYAFSESFVLPISHDEVVHGKGTLLQKMAG 632 Query: 619 DEWRRFANVRAYYGFMWTHPGKKLLFMGSEFGQPDEFDHDKSPYWHLLNEHGDHPAGHRQ 678 D W+RFAN+RAY GFMWTHPGKKLLFMG EF + EFD D+SP+W LL++ A HR Sbjct: 633 DAWQRFANLRAYLGFMWTHPGKKLLFMGCEFAETREFDPDRSPHWDLLDD-----AHHRG 687 Query: 679 LRQLVSDLNHLYRSEPALHQRDCRADGFSWAVGDDSVNSVLAYFRYADEGPPVLIVVNLT 738 +++LV DLN LY PAL++ D A GFSW VG+D +NSV AY R D+ PVL+V+N+T Sbjct: 688 VQRLVRDLNRLYAGTPALYRHDTEAKGFSWVVGNDELNSVFAYLRQTDDEAPVLVVINMT 747 Query: 739 PVPRTHYRIGVPPIAPGHHGVWREVLNTDAAVYGGSNSGNMGQVQVQEFGWHGHPTSIEL 798 PVPR HYRIGVPP W E+LNTDA YGGS+ GN GQ++ Q HGH +S+ L Sbjct: 748 PVPRHHYRIGVPP-GQTQQDRWEEILNTDADCYGGSHLGNGGQLRAQAQACHGHASSLVL 806 Query: 799 NLPPLSTLILRR 810 LPPLSTL+LRR Sbjct: 807 TLPPLSTLVLRR 818 >gi|300311816|ref|YP_003775908.1| malto-oligosyltrehalose trehalohydrolase [Herbaspirillum seropedicae SmR1] Length = 616 Score = 118 bits (296), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 98/310 (31%), Positives = 136/310 (43%), Gaps = 41/310 (13%) Query: 205 TRFAVWAPNAERVSVVGDFNQWDGRRHVMRLRHESGVWELFIPRIGPGDVYKY--EILGR 262 RF VWAP A V+V D R + + E W PG Y+Y + + + Sbjct: 21 VRFRVWAPGARSVAV--DITG----RGRLPMDAEPQGWFALETACDPGATYRYVFDTISQ 74 Query: 263 DGALLPLKADPVARATEPPPATASVVADARPYLWQDQQWMAERAQRQSLSAPMSIYELHP 322 + P DP +R S+V DA+ Y+WQ QW + L YELHP Sbjct: 75 GELVAP---DPASRGQAGDVFDPSLVVDAQAYVWQYPQWRGRPWEETVL------YELHP 125 Query: 323 ASWRRIPEQAHRNLSWDELADELIPYVVGMGFTHIELMPVMEHPFGGSWGYQPISMFAPS 382 + R ELA+ +G T IELMPV E P +WGY + FAP Sbjct: 126 GAMGGFAGIRQR---LAELAE--------LGITAIELMPVAEFPGAHNWGYDGVLPFAPD 174 Query: 383 ARFGKPERFAAFVDRCHQAGIGVLLDWVPAHFPSDQHGLAQFDGTALYEHADPREGFHQD 442 A +G PE+ A +D H G+ V LD V HF D + Y HA E F QD Sbjct: 175 ASYGTPEQLKALIDTAHGLGMMVFLDVVYNHFGPDGN----------YLHAYAPEFFRQD 224 Query: 443 WNTL---IFNLGRNEVRGFLIASALEWIEHFHVDGLRVDAVASMLYRDYSRKSGEWVPNI 499 TL + R EV F +AL W++ + DGLR+DAV ++ + + G+ + Sbjct: 225 MQTLWGATIDFRRPEVDDFFTQNALYWLQEYRFDGLRLDAVHAICEPAWLERLGQRIRTE 284 Query: 500 HGGRENLEAV 509 GG+ ++ V Sbjct: 285 IGGQRHVHLV 294 >gi|300311817|ref|YP_003775909.1| glycogen debranching enzyme protein [Herbaspirillum seropedicae SmR1] Length = 753 Score = 62.4 bits (150), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 59/195 (30%), Positives = 82/195 (42%), Gaps = 27/195 (13%) Query: 317 IYELHPASWRRIPEQA--HRNLSWDELAD-ELIPYVVGMGFTHIELMPV---------ME 364 IYE H R+ E H ++ LAD I +++ +G T +ELMPV +E Sbjct: 167 IYEAHVKGISRLREDLLPHERGTFAALADPAFIDHLLRLGVTTLELMPVHAFLQDSFLVE 226 Query: 365 HPFGGSWGYQPISMFAPSARF---GKPERFAAFVDRCHQAGIGVLLDWVPAHFPSDQH-- 419 WGY +S FAP + G P V R H AGI V+LD V H Sbjct: 227 RGLHNYWGYSTLSYFAPEPGYLSMGDPNELRMAVRRLHSAGIEVILDVVYNHTSCGSELG 286 Query: 420 ---GLAQFDGTALYEHADPREGFHQD----WNTLIFNLGRNEVRGFLIASALEWIEHFHV 472 D + + +E +H + NTL NL V ++ S W + FH+ Sbjct: 287 PTLSFRGLDNASYFRLLPGQERYHINDTGCGNTL--NLSHPRVLQMVLDSLRYWAQSFHI 344 Query: 473 DGLRVDAVASMLYRD 487 DG R D + S L R+ Sbjct: 345 DGFRFD-LGSTLGRE 358 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,806,546 Number of extensions: 128733 Number of successful extensions: 335 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 325 Number of HSP's successfully gapped: 4 Length of query: 812 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 719 Effective length of database: 1,179,515 Effective search space: 848071285 Effective search space used: 848071285 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 135 (56.6 bits)