BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0052.p (755 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311817|ref|YP_003775909.1| glycogen debranching enzyme pro... 1427 0.0 gi|300311816|ref|YP_003775908.1| malto-oligosyltrehalose trehalo... 86 7e-18 gi|300311818|ref|YP_003775910.1| 1,4-alpha-glucan branching prot... 65 2e-11 >gi|300311817|ref|YP_003775909.1| glycogen debranching enzyme protein [Herbaspirillum seropedicae SmR1] Length = 753 Score = 1427 bits (3695), Expect = 0.0, Method: Compositional matrix adjust. Identities = 684/754 (90%), Positives = 720/754 (95%), Gaps = 2/754 (0%) Query: 1 MSVAFPDRLMPGLPYPLGATSDGLGVNFAVFSANATRMDLCLFDPSGKRELKRITLPECT 60 MSVAFPDRLMPGLPYPLGA+SDGLGVNFAVFSA+ATRMDLCLFDPSGKRELKRI LPECT Sbjct: 1 MSVAFPDRLMPGLPYPLGASSDGLGVNFAVFSAHATRMDLCLFDPSGKRELKRIALPECT 60 Query: 61 DEVWHGYLPGAAPGLVYGFRAHGPYEPTKGHRFNPNKLLLDPYAKRLVGQVRWTDALYGY 120 DEVWHGYLPGAAPGLVYGFRAHGPYEP KGHRFNPNKLLLDPYAKRLVGQVRWTDALYGY Sbjct: 61 DEVWHGYLPGAAPGLVYGFRAHGPYEPNKGHRFNPNKLLLDPYAKRLVGQVRWTDALYGY 120 Query: 121 RLHSARADLSFDRRDSAAAMPKAMVTDDAFVWEHDRLPQVPWNKTVIYEAHVKGISKMRE 180 RLHS RADLSFDRRDSAAAMPKAMVT+DAFVWEHDRLPQVPWNKTVIYEAHVKGIS++RE Sbjct: 121 RLHSPRADLSFDRRDSAAAMPKAMVTEDAFVWEHDRLPQVPWNKTVIYEAHVKGISRLRE 180 Query: 181 DLLPHERGTFAALSDPAFIDHLLRLGVTTLELMPVHAFLQDSFLIERGLSNYWGYSTLSY 240 DLLPHERGTFAAL+DPAFIDHLLRLGVTTLELMPVHAFLQDSFL+ERGL NYWGYSTLSY Sbjct: 181 DLLPHERGTFAALADPAFIDHLLRLGVTTLELMPVHAFLQDSFLVERGLHNYWGYSTLSY 240 Query: 241 FAPEPSYMSMGDPNELRMAVRRLHSAGIEVILDVVYNHTSCGSELGPTLSFRGLDNASYF 300 FAPEP Y+SMGDPNELRMAVRRLHSAGIEVILDVVYNHTSCGSELGPTLSFRGLDNASYF Sbjct: 241 FAPEPGYLSMGDPNELRMAVRRLHSAGIEVILDVVYNHTSCGSELGPTLSFRGLDNASYF 300 Query: 301 RLLPGQERYHINDTGCGNTLNLSHPRVLQMVMDSLRYWAQSFHIDGFRFDLGSTLGREPH 360 RLLPGQERYHINDTGCGNTLNLSHPRVLQMV+DSLRYWAQSFHIDGFRFDLGSTLGREPH Sbjct: 301 RLLPGQERYHINDTGCGNTLNLSHPRVLQMVLDSLRYWAQSFHIDGFRFDLGSTLGREPH 360 Query: 361 GFDPGSGFFDALLQDPVLSKLKLISEPWDIGPGGYQLGNHPARFGEWNDKFRDDMRKFWK 420 GFDPGSGFFDALLQDPVLS+LKLISEPWDIGPGGYQLGNHPARFGEWNDKFRDDMRKFWK Sbjct: 361 GFDPGSGFFDALLQDPVLSRLKLISEPWDIGPGGYQLGNHPARFGEWNDKFRDDMRKFWK 420 Query: 421 GDEAMRGGFAQRMMGSAELFEHNRRKPWATVNFIASHDGFTLRDLVSYNGKHNDANGENN 480 GDEAMRG FAQRMMGSAELFEH+RRKPWA++NFIASHDGFTLRDLVSYNGKHN+ANGENN Sbjct: 421 GDEAMRGAFAQRMMGSAELFEHHRRKPWASINFIASHDGFTLRDLVSYNGKHNEANGENN 480 Query: 481 NDGHNDNASYNWGAEGETDDPAIQATRACVQRSMLASTLLAQGTPMLLAGDEFNRSQGGN 540 NDGHNDNASYNWGAEG+TDD AI A RACVQR+MLASTLLAQGTPMLLAGDEF+RSQGGN Sbjct: 481 NDGHNDNASYNWGAEGDTDDAAILAKRACVQRAMLASTLLAQGTPMLLAGDEFHRSQGGN 540 Query: 541 NNAYCQDNDISWINWEQARSPEGAALTAFVGRVTAIRRDFDVLRASYFLHGNQMVADGLP 600 NNAYCQDN+ISW+NW+QARSPEGAAL+AFVGRVTAIRRDF+VLRASYFLHG+QMVADGLP Sbjct: 541 NNAYCQDNEISWVNWDQARSPEGAALSAFVGRVTAIRRDFEVLRASYFLHGSQMVADGLP 600 Query: 601 DVAWFDESAQPVTSEDWNNPVARAITLQLAGKRLEPHKQLSSRLHDGAGAAADGPGEAQE 660 DVAWFDE+A+PV+SEDWNNPVARAITLQLAGK E HKQLSSRLHD +D P EA E Sbjct: 601 DVAWFDEAARPVSSEDWNNPVARAITLQLAGKPQESHKQLSSRLHD--AGQSDSPAEAAE 658 Query: 661 VPPDVLMIFVNAGHEPIEFSFPRTEGEYFLLLDCAQPTGMPEGEGGQVKVDGSASVQVQA 720 PDVLMIFVNAGHE I+F FPRTEG+Y LLLD AQ TG+P GE ++ + GSA+VQV A Sbjct: 659 ATPDVLMIFVNAGHEAIDFHFPRTEGDYHLLLDTAQDTGIPTGEDVRLVLPGSATVQVPA 718 Query: 721 HTLQVFANRPLHLSSAIYPLAANEWIKRNEWGER 754 H+LQVFANRPLHL+SAIYPLAANEWI RNEWGER Sbjct: 719 HSLQVFANRPLHLASAIYPLAANEWIARNEWGER 752 >gi|300311816|ref|YP_003775908.1| malto-oligosyltrehalose trehalohydrolase [Herbaspirillum seropedicae SmR1] Length = 616 Score = 86.3 bits (212), Expect = 7e-18, Method: Compositional matrix adjust. Identities = 65/210 (30%), Positives = 89/210 (42%), Gaps = 40/210 (19%) Query: 141 PKAMVTDDAFVWEHDRLPQVPWNKTVIYEAHVKGISKMREDLLPHERGTFAALSDPAFID 200 P +V A+VW++ + PW +TV+YE H P G FA + + Sbjct: 94 PSLVVDAQAYVWQYPQWRGRPWEETVLYELH------------PGAMGGFAGIRQR--LA 139 Query: 201 HLLRLGVTTLELMPVHAFLQDSFLIERGLSNYWGYSTLSYFAPEPSYMSMGDPNELRMAV 260 L LG+T +ELMPV F G N WGY + FAP+ SY G P +L+ + Sbjct: 140 ELAELGITAIELMPVAEF--------PGAHN-WGYDGVLPFAPDASY---GTPEQLKALI 187 Query: 261 RRLHSAGIEVILDVVYNHTSCGSELGPTLSFRGLDNASYFRLLPGQERYHINDTGCGNTL 320 H G+ V LDVVYNH GP ++ +FR T G T+ Sbjct: 188 DTAHGLGMMVFLDVVYNH------FGPDGNYLHAYAPEFFR--------QDMQTLWGATI 233 Query: 321 NLSHPRVLQMVMDSLRYWAQSFHIDGFRFD 350 + P V + YW Q + DG R D Sbjct: 234 DFRRPEVDDFFTQNALYWLQEYRFDGLRLD 263 >gi|300311818|ref|YP_003775910.1| 1,4-alpha-glucan branching protein [Herbaspirillum seropedicae SmR1] Length = 804 Score = 65.1 bits (157), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 91/344 (26%), Positives = 132/344 (38%), Gaps = 63/344 (18%) Query: 22 DGL-GVNFAVFSANATRMDLC--LFDPSGKRELKRITLPECTDEVWHGYLPGAAPGLVYG 78 DG+ G FAV++ NA R+ + G+R + R+ VW ++P PG VY Sbjct: 192 DGVPGTRFAVWAPNAERVSVVGDFNQWDGRRHVMRL---RHEPGVWELFIPRIGPGDVYK 248 Query: 79 FRAHGPYEPTKGHRFNPNKLLLDPYAKRLVGQVRWTDALYGYRLHSARADLSFDRRDSAA 138 + G +G P K DP A RA S Sbjct: 249 YEIRG-----RGGVLLPLKA--DPVA---------------------RATEPPPATASVV 280 Query: 139 AMPKAMVTDDAFVWEHDRLPQVPWNKTV-IYEAHVKGISKMREDLLPHERGTFAALSDPA 197 A + V D W +R + + + IYE H ++ E H + L+D Sbjct: 281 ADARPYVWQDQ-QWMAERAQRQSLSAPMSIYEVHPASWRRVPEQ--DHRNLNWDELAD-Q 336 Query: 198 FIDHLLRLGVTTLELMPVHAFLQDSFLIERGLSNYWGYSTLSYFAPEPSYMSMGDPNELR 257 I +++ +G T +ELMPV +E WGY +S FAP + G P Sbjct: 337 LIPYVVGMGFTHIELMPV---------MEHPFGGSWGYQPISMFAPSARF---GKPERFA 384 Query: 258 MAVRRLHSAGIEVILDVVYNHTSCGSELGPTLSFRGLDNASYFRLLPGQERYHINDTGCG 317 V R H AGI V+LD V H D + + +E +H + Sbjct: 385 AFVDRCHQAGIGVLLDWVPAHFPSDQH-----GLAQFDGTALYEHADPREGFHQD----W 435 Query: 318 NTL--NLSHPRVLQMVMDSLRYWAQSFHIDGFRFD-LGSTLGRE 358 NTL NL V ++ S W + FH+DG R D + S L R+ Sbjct: 436 NTLIFNLGRNEVRGFLIASALEWLEHFHVDGLRVDAVASMLYRD 479 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,537,900 Number of extensions: 112456 Number of successful extensions: 249 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 247 Number of HSP's successfully gapped: 3 Length of query: 755 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 663 Effective length of database: 1,184,250 Effective search space: 785157750 Effective search space used: 785157750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)