BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0051.p (626 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311816|ref|YP_003775908.1| malto-oligosyltrehalose trehalo... 1054 0.0 gi|300311818|ref|YP_003775910.1| 1,4-alpha-glucan branching prot... 117 4e-27 gi|300311642|ref|YP_003775734.1| 1,4-alpha-glucan branching prot... 100 4e-22 gi|300311817|ref|YP_003775909.1| glycogen debranching enzyme pro... 96 1e-20 >gi|300311816|ref|YP_003775908.1| malto-oligosyltrehalose trehalohydrolase [Herbaspirillum seropedicae SmR1] Length = 616 Score = 1054 bits (2726), Expect = 0.0, Method: Compositional matrix adjust. Identities = 527/615 (85%), Positives = 558/615 (90%), Gaps = 14/615 (2%) Query: 2 KLPFGAQVTAPGQVRFRVWAPGADAVAVDITGRGRLPMTAEDAASPAWYVLETECDPGTL 61 LPFGAQVT PG+VRFRVWAPGA +VAVDITGRGRLPM DA W+ LET CDPG Sbjct: 8 NLPFGAQVTEPGRVRFRVWAPGARSVAVDITGRGRLPM---DAEPQGWFALETACDPGAT 64 Query: 62 YRYVFQTREQGEVSAPDPASRGQAGDVFDPSVVVDPEAFLWQHTNWQGRPWEETVLYELH 121 YRYVF T QGE+ APDPASRGQAGDVFDPS+VVD +A++WQ+ W+GRPWEETVLYELH Sbjct: 65 YRYVFDTISQGELVAPDPASRGQAGDVFDPSLVVDAQAYVWQYPQWRGRPWEETVLYELH 124 Query: 122 PGAMGGFAGIQQRLAELAELGITAIELMPVAEFPGAHNWGYDGVLPFAPDASYGTPDQLK 181 PGAMGGFAGI+QRLAELAELGITAIELMPVAEFPGAHNWGYDGVLPFAPDASYGTP+QLK Sbjct: 125 PGAMGGFAGIRQRLAELAELGITAIELMPVAEFPGAHNWGYDGVLPFAPDASYGTPEQLK 184 Query: 182 ALIDTAHGLGMMVFLDVVYNHFGPDGNYLHSYAPDFFRHDMNTLWGATIDFRRPEVADFF 241 ALIDTAHGLGMMVFLDVVYNHFGPDGNYLH+YAP+FFR DM TLWGATIDFRRPEV DFF Sbjct: 185 ALIDTAHGLGMMVFLDVVYNHFGPDGNYLHAYAPEFFRQDMQTLWGATIDFRRPEVDDFF 244 Query: 242 TQNALYWLQEYRFDGLRLDAVHAICEPDWLVALGQRVRAEIGNERHVHLVLEHDGNAAHL 301 TQNALYWLQEYRFDGLRLDAVHAICEP WL LGQR+R EIG +RHVHLVLEHDGNAAHL Sbjct: 245 TQNALYWLQEYRFDGLRLDAVHAICEPAWLERLGQRIRTEIGGQRHVHLVLEHDGNAAHL 304 Query: 302 LGYTAAVEAEAEVAEIGQVRERSSHVYDAQWNDDAHHVLHVLLTGEDSGYYSAYAEAPAE 361 LG+ + RSSHVYDAQWNDDAHHVLHVLLTGEDSGYY+AYAE PA Sbjct: 305 LGHGHPPD-----------DGRSSHVYDAQWNDDAHHVLHVLLTGEDSGYYAAYAEEPAA 353 Query: 362 KLARCLQQGFVYQGEVSPYSGEPRGEPSAELPPTAFVNFLQNHDQIGNRAFGERLTVLAH 421 KLARCLQQGFVYQGEVSPYSGEPRGEPSA+LPPTAFVNFLQNHDQIGNRAFGERLTVLAH Sbjct: 354 KLARCLQQGFVYQGEVSPYSGEPRGEPSADLPPTAFVNFLQNHDQIGNRAFGERLTVLAH 413 Query: 422 PLALHAAQALILLAPQVPMLFMGEEFCAVQPFLYFTSHRDEELVKAVREGRRREFARFPQ 481 PLALHAAQAL+LLAPQ+PMLFMGEEFCAVQPFLYFTSHRDE LVKAVREGRRREFARFPQ Sbjct: 414 PLALHAAQALVLLAPQIPMLFMGEEFCAVQPFLYFTSHRDEALVKAVREGRRREFARFPQ 473 Query: 482 FADPKLLEQIPDPNSFATFVSSIPEPGPAPGSAACLRRVAELLHIRKLHIVPYLRGAKSL 541 FADP LLEQIPDPNSFATFVSSIPEPGPAPGSAACLRRVAELLHIRKLHI PYLRGA++L Sbjct: 474 FADPALLEQIPDPNSFATFVSSIPEPGPAPGSAACLRRVAELLHIRKLHIAPYLRGARAL 533 Query: 542 EARAIGPKAVHARWQLANGSVLNITVNLDEAPLQEALDDLARAAGADVLFDSGGALEALA 601 EARAIGPKAVHARW+L+NGSVLNITVNL E +QE+LD LA++AGADVLFDSGGALEA+A Sbjct: 534 EARAIGPKAVHARWRLSNGSVLNITVNLAEQAVQESLDQLAQSAGADVLFDSGGALEAVA 593 Query: 602 AGSFPGHAILALREP 616 G FPGHAILALREP Sbjct: 594 EGRFPGHAILALREP 608 >gi|300311818|ref|YP_003775910.1| 1,4-alpha-glucan branching protein [Herbaspirillum seropedicae SmR1] Length = 804 Score = 117 bits (292), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 95/297 (31%), Positives = 130/297 (43%), Gaps = 38/297 (12%) Query: 15 VRFRVWAPGADAVAV--DITG-RGRLPMTAEDAASPAWYVLETECDPGTLYRYVFQTREQ 71 RF VWAP A+ V+V D GR + W + PG +Y+Y + R + Sbjct: 197 TRFAVWAPNAERVSVVGDFNQWDGRRHVMRLRHEPGVWELFIPRIGPGDVYKY--EIRGR 254 Query: 72 GEVSAP---DPASRGQAGDVFDPSVVVDPEAFLWQHTNWQGRPWEETVL------YELHP 122 G V P DP +R SVV D ++WQ W + L YE+HP Sbjct: 255 GGVLLPLKADPVARATEPPPATASVVADARPYVWQDQQWMAERAQRQSLSAPMSIYEVHP 314 Query: 123 GAMGGFAGIQQR---LAELAE--------LGITAIELMPVAEFPGAHNWGYDGVLPFAPD 171 + R ELA+ +G T IELMPV E P +WGY + FAP Sbjct: 315 ASWRRVPEQDHRNLNWDELADQLIPYVVGMGFTHIELMPVMEHPFGGSWGYQPISMFAPS 374 Query: 172 ASYGTPDQLKALIDTAHGLGMMVFLDVVYNHFGPDGN----------YLHSYAPDFFRHD 221 A +G P++ A +D H G+ V LD V HF D + Y H+ + F D Sbjct: 375 ARFGKPERFAAFVDRCHQAGIGVLLDWVPAHFPSDQHGLAQFDGTALYEHADPREGFHQD 434 Query: 222 MNTLWGATIDFRRPEVADFFTQNALYWLQEYRFDGLRLDAVHAICEPDWLVALGQRV 278 NTL + R EV F +AL WL+ + DGLR+DAV ++ D+ G+ V Sbjct: 435 WNTL---IFNLGRNEVRGFLIASALEWLEHFHVDGLRVDAVASMLYRDYSRKEGEWV 488 >gi|300311642|ref|YP_003775734.1| 1,4-alpha-glucan branching protein [Herbaspirillum seropedicae SmR1] Length = 819 Score = 100 bits (248), Expect = 4e-22, Method: Compositional matrix adjust. Identities = 94/309 (30%), Positives = 128/309 (41%), Gaps = 44/309 (14%) Query: 8 QVTAPGQVRFRVWAPGADAVAV-----DITGRGRLPMTAEDAASPAWYVLETECDPGTLY 62 Q+ VRF VWAP A V+V GR R PM +A W + G LY Sbjct: 212 QIDGVEGVRFAVWAPNAQRVSVIGDFNQWDGR-RNPMRLRQSAG-VWELFVPRLGDGALY 269 Query: 63 RYVFQTREQGEV--SAPDPASRGQAGDVFDPSVVVDPEAFL----WQHTNW-QGRPWEET 115 +Y GE+ DP +R S V+D + WQ +W Q R +T Sbjct: 270 KYEI-IGPHGELLPGKADPMARATEVPPATASRVIDDSKLVSGRRWQDAHWLQQRAQGQT 328 Query: 116 -----VLYELHPGAMGGFAGIQQRLAELAEL-----------GITAIELMPVAEFPGAHN 159 +YE+H + G R + EL G T +EL+PV E P + Sbjct: 329 RQAPMSIYEVHAASWLRLPGEGNRNLDWNELADRLIPYVKGMGFTHVELLPVMEHPFGGS 388 Query: 160 WGYDGVLPFAPDASYGTPDQLKALIDTAHGLGMMVFLDVVYNHFGPDGN----------Y 209 WGY + FAP A +G P + +D H G+ V LD V HF D + Y Sbjct: 389 WGYQPLSQFAPSARFGPPAEFATFVDACHRAGIGVLLDWVPAHFPSDPHGLAYFDGTPLY 448 Query: 210 LHSYAPDFFRHDMNTLWGATIDFRRPEVADFFTQNALYWLQEYRFDGLRLDAVHAICEPD 269 H + D NT + R EV F +AL WL+ + DGLR+DAV ++ D Sbjct: 449 EHGDPREGIHQDWNT---HIYNLGRHEVRGFLIASALEWLEHFHVDGLRVDAVASMLYRD 505 Query: 270 WLVALGQRV 278 + + GQ V Sbjct: 506 YSRSSGQWV 514 >gi|300311817|ref|YP_003775909.1| glycogen debranching enzyme protein [Herbaspirillum seropedicae SmR1] Length = 753 Score = 95.5 bits (236), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 67/210 (31%), Positives = 92/210 (43%), Gaps = 40/210 (19%) Query: 91 PSVVVDPEAFLWQHTNWQGRPWEETVLYELH------------PGAMGGFAGIQQ--RLA 136 P +V +AF+W+H PW +TV+YE H P G FA + + Sbjct: 141 PKAMVTEDAFVWEHDRLPQVPWNKTVIYEAHVKGISRLREDLLPHERGTFAALADPAFID 200 Query: 137 ELAELGITAIELMPVAEF--------PGAHN-WGYDGVLPFAPDASY---GTPDQLKALI 184 L LG+T +ELMPV F G HN WGY + FAP+ Y G P++L+ + Sbjct: 201 HLLRLGVTTLELMPVHAFLQDSFLVERGLHNYWGYSTLSYFAPEPGYLSMGDPNELRMAV 260 Query: 185 DTAHGLGMMVFLDVVYNH------FGPDGNYLHSYAPDFFR--------HDMNTLWGATI 230 H G+ V LDVVYNH GP ++ +FR H +T G T+ Sbjct: 261 RRLHSAGIEVILDVVYNHTSCGSELGPTLSFRGLDNASYFRLLPGQERYHINDTGCGNTL 320 Query: 231 DFRRPEVADFFTQNALYWLQEYRFDGLRLD 260 + P V + YW Q + DG R D Sbjct: 321 NLSHPRVLQMVLDSLRYWAQSFHIDGFRFD 350 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,037,352 Number of extensions: 90853 Number of successful extensions: 270 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 261 Number of HSP's successfully gapped: 4 Length of query: 626 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 535 Effective length of database: 1,188,985 Effective search space: 636106975 Effective search space used: 636106975 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)