BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0047.p (553 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311812|ref|YP_003775904.1| glucose-6-phosphate isomerase [... 926 0.0 gi|300310432|ref|YP_003774524.1| glucose-6-phosphate isomerase [... 539 e-154 >gi|300311812|ref|YP_003775904.1| glucose-6-phosphate isomerase [Herbaspirillum seropedicae SmR1] Length = 570 Score = 926 bits (2392), Expect = 0.0, Method: Compositional matrix adjust. Identities = 441/556 (79%), Positives = 484/556 (87%), Gaps = 9/556 (1%) Query: 1 MTKLPAADLQLPLFTYGQRQSLTEKSAWIALQEHHQYSARHFRLERLFAADDKRGEKLVA 60 MT LPAADLQLPLF Y QR+SLT KSAW +LQEH++ ARHF+L RLFAADDKRGE L A Sbjct: 1 MTTLPAADLQLPLFVYAQRESLTGKSAWRSLQEHYRDRARHFQLARLFAADDKRGESLTA 60 Query: 61 EAAGIYLDYSKNLVTTETMTLLMNLARLIS---------XSPPINATEQRSVLHTALRMP 111 EA GIYLDYSKNLVT+ETM LLM LAR IN TE RSVLHTALR P Sbjct: 61 EAVGIYLDYSKNLVTSETMALLMQLARQCGLPDRIEAMFRGDRINLTENRSVLHTALRKP 120 Query: 112 ADARLVVEGEDVIASVQEVLSRMEAFAEQVRSGAWTGHTGRRIRHVVNIGIGGSDLGPMM 171 A RLVV+G+DV+A VQ+VL RME FA QVRSG W GHTG IRHVVNIGIGGSDLGPMM Sbjct: 121 ASERLVVDGQDVVAEVQQVLRRMEGFARQVRSGQWLGHTGLPIRHVVNIGIGGSDLGPMM 180 Query: 172 AYEALRPYAQPDLSLHFLSDVDGAALLETVRHLPAEETLFIVCSKTFTTLETTTNARAAR 231 AYEALRPY+ DL+LHF+SDVDGAA+L+TVR LPAE+TLFIVCSKTFTTLET TNA AAR Sbjct: 181 AYEALRPYSHDDLTLHFVSDVDGAAMLDTVRGLPAEQTLFIVCSKTFTTLETITNATAAR 240 Query: 232 DWLVRQLGEEAAVARHFVGVTTNIEEASRFGIDPQNMFGFWDWVGGRYSMTSAIGLSTMI 291 WLV+QLG E AVARHFVGVTTNIEEA RFGID NMFGFWDWVGGRYSMTSAIGLSTM+ Sbjct: 241 AWLVQQLGAEEAVARHFVGVTTNIEEAQRFGIDADNMFGFWDWVGGRYSMTSAIGLSTMV 300 Query: 292 AIGPEHFREMLAGFHAMDEHFRTAPLEANVPAILGMLTVWYTNFFGATSQAVLPYSQYLK 351 AIGPEHFR ML GFHAMDEHFR+APLE+N+P ILGMLTVWYTNFFGA SQAVLPYSQ+LK Sbjct: 301 AIGPEHFRAMLEGFHAMDEHFRSAPLESNLPVILGMLTVWYTNFFGAASQAVLPYSQHLK 360 Query: 352 RLPAYLQQLTMESNGKQVNLQGDAVDYQTSPVYWGDNGINGQHSFYQLLHQGTQMIPCDF 411 RLPAYLQQLTMESNGK + + G+ VDYQTSPVYWGDNGINGQHSFYQ+LHQGTQ++PCDF Sbjct: 361 RLPAYLQQLTMESNGKHITVNGEPVDYQTSPVYWGDNGINGQHSFYQMLHQGTQLVPCDF 420 Query: 412 IGFCQPVEALGDHHALLMANMFAQAEALAFGKNAQRVREEGTPESLTPHRVFPGNRPSNS 471 IG CQP +ALG H LLMANMFAQ+EALAFGK+A +VR+EGTPE+L PHRVF GNRPSNS Sbjct: 421 IGLCQPQQALGQQHELLMANMFAQSEALAFGKDADQVRQEGTPEALVPHRVFEGNRPSNS 480 Query: 472 MLVERLTPHSLGTLVALYEHSVFTQGVIWNIDSFDQWGVELGKQLAKRTARQLLDPAEPL 531 ML+++LTP+SLGTLVALYEHSVFTQGV+WNIDSFDQWGVELGKQLAKRTAR+L+D +PL Sbjct: 481 MLLQQLTPYSLGTLVALYEHSVFTQGVVWNIDSFDQWGVELGKQLAKRTARELIDHQQPL 540 Query: 532 HHDSSTNALIRRYRSA 547 HHDSSTNALIRRYR+ Sbjct: 541 HHDSSTNALIRRYRAC 556 >gi|300310432|ref|YP_003774524.1| glucose-6-phosphate isomerase [Herbaspirillum seropedicae SmR1] Length = 554 Score = 539 bits (1389), Expect = e-154, Method: Compositional matrix adjust. Identities = 282/545 (51%), Positives = 350/545 (64%), Gaps = 20/545 (3%) Query: 21 SLTEKSAWIALQEHHQYSARHFRLERLFAADDKRGEKLVAEAAGIYLDYSKNLVTTETMT 80 +LT+ +A+ ALQ HH A + + LFA R E++ AAG+ LDYSKN + +ET+ Sbjct: 5 ALTDTAAFKALQHHHA-KAVTWHMRELFAQQPDRFERMHVRAAGLLLDYSKNRIESETLA 63 Query: 81 LLMNLAR---------LISXSPPINATEQRSVLHTALRMPADA-RLVVEGEDVIASVQEV 130 LLM+LAR + IN TE R+VLHTALR PA A V+G+ V V V Sbjct: 64 LLMSLARERGVEALREAMFRGEKINFTEHRAVLHTALRAPAQACDAQVDGQPVSQEVHAV 123 Query: 131 LSRMEAFAEQVRSGAWTGHTGRRIRHVVNIGIGGSDLGPMMAYEALRPYAQPDLSLHFLS 190 L + F+E+VRSGAW G+TGR I +VNIGIGGS LGP M ALR Y L+ HF+S Sbjct: 124 LDHIRDFSERVRSGAWRGYTGREITDIVNIGIGGSFLGPQMVCTALRNYRHARLTTHFVS 183 Query: 191 DVDGAALLETVRHLPAEETLFIVCSKTFTTLETTTNARAARDWLVRQLGEEAAVARHFVG 250 +VDG L E + + E TLFIV SKTFTT ET NA++AR W +R G++A +ARHFV Sbjct: 184 NVDGHDLDEVLSRVDPETTLFIVASKTFTTQETMLNAKSARAWFLRH-GQQADLARHFVA 242 Query: 251 VTTNIEEASRFGIDPQNMFGFWDWVGGRYSMTSAIGLSTMIAIGPEHFREMLAGFHAMDE 310 V+TN E FGIDP NMF FWDWVGGRYS+ SAIGL + IG EHF LAG HAMD Sbjct: 243 VSTNAAEVESFGIDPDNMFPFWDWVGGRYSVWSAIGLPIALLIGFEHFSAFLAGAHAMDR 302 Query: 311 HFRTAPLEANVPAILGMLTVWYTNFFGATSQAVLPYSQYLKRLPAYLQQLTMESNGKQVN 370 HFR APLEAN+P I ++ +W NFF + S +V PY Q+L+ PAYLQQL MESNGK++ Sbjct: 303 HFRDAPLEANMPVIQALIGIWNRNFFNSESLSVAPYHQWLRYFPAYLQQLDMESNGKRIT 362 Query: 371 LQGDAVDYQTSPVYWGDNGINGQHSFYQLLHQGTQMIPCDFIGFCQPVEALGDHHALLMA 430 G V T P WGD G NGQH+F+QLLHQGT + P DFI +P + +H +L+A Sbjct: 363 RDGTPVGVATCPFIWGDVGTNGQHAFFQLLHQGTNVTPIDFIAVLKPSHGIDEHQDVLLA 422 Query: 431 NMFAQAEALAFGKNAQRVR--------EEGTPESLTPHRVFPGNRPSNSMLVERLTPHSL 482 N FAQ+EA GK A VR + +L PH+ F GNRPSN++L+ L+PH+L Sbjct: 423 NCFAQSEAFMRGKQADEVRADLERQGVDAAELAALLPHKTFDGNRPSNTLLLPELSPHAL 482 Query: 483 GTLVALYEHSVFTQGVIWNIDSFDQWGVELGKQLAKRTARQLLDPAEPLHHDSSTNALIR 542 G ++ALYEH F QG IW I+SFDQWGVELGK LAK +L P HD+STNALI Sbjct: 483 GAMIALYEHKTFVQGAIWGINSFDQWGVELGKVLAKTIHGELRSEPNPALHDASTNALIA 542 Query: 543 RYRSA 547 R A Sbjct: 543 LARQA 547 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,739,662 Number of extensions: 71923 Number of successful extensions: 208 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 201 Number of HSP's successfully gapped: 2 Length of query: 553 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 463 Effective length of database: 1,193,720 Effective search space: 552692360 Effective search space used: 552692360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)