BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0044.p (499 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311810|ref|YP_003775902.1| two component sensor histidine ... 683 0.0 gi|300311887|ref|YP_003775979.1| two component sensor histidine ... 116 4e-27 gi|300310566|ref|YP_003774658.1| Hpt sensor hybrid histidine kin... 88 2e-18 gi|300313070|ref|YP_003777162.1| two component sensor histidine ... 86 5e-18 gi|300314018|ref|YP_003778110.1| two component sensor histidine ... 76 6e-15 >gi|300311810|ref|YP_003775902.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 535 Score = 683 bits (1763), Expect = 0.0, Method: Compositional matrix adjust. Identities = 345/532 (64%), Positives = 406/532 (76%), Gaps = 37/532 (6%) Query: 3 MRLLTKGLLIVAIPSLFEVLLLGALFKFQADAEDAEGWASHSLQVMNKAAELREPMLLEA 62 MRLLTKGLLIVAIP LFE++LL ALFK QADA DAE WA+HS +V+NKAA++REP+LLE+ Sbjct: 1 MRLLTKGLLIVAIPGLFEIVLLAALFKSQADAADAERWAAHSAEVINKAADVREPLLLES 60 Query: 63 ARVRNAVLLDLPDTLSRGDLWADIEVKTGELARLVADNASQSAAVRTVHESIASYRSWDD 122 AR+RNA+++D P +SR +LW D+E+K G+LARLV+DN QS +V+ + E + +R W D Sbjct: 61 ARIRNAIIIDDPSPISRPELWTDLEIKLGDLARLVSDNPEQSHSVQALRELVGQFRVWAD 120 Query: 123 SARERLMRGQRDDLIRQLRDEQGPHRLSTFLNLLDNFVAEERRLGAERSERLQAARRSQT 182 SARER+ RGQRD+L+RQLRDEQGPHRLS FLN LD F+ EER LG+ R++ LQ+AR++QT Sbjct: 121 SARERMQRGQRDELVRQLRDEQGPHRLSDFLNRLDGFLNEERHLGSLRNQALQSARQTQT 180 Query: 183 WLLILAVLVSIVTAGLASLAFMRNISTRIGVLIGNARRLXSA------------------ 224 LI AV+ SI+ A LAS+AF R+I RI VLI NARRL Sbjct: 181 AWLIAAVIGSILAAALASVAFTRSIGGRISVLIVNARRLADGLPLAARVKGHDEISLLDD 240 Query: 225 -------------------RARARARARELADVNSDLRQQTQDNEMFIYSVSHDLRSPLV 265 R++ RAREL ++N+DL QQTQDNEMFIYSVSHDLRSPLV Sbjct: 241 ALHRSSERLQEASQQSEQYRSQLERRARELTELNADLLQQTQDNEMFIYSVSHDLRSPLV 300 Query: 266 NLQGFSKEITHATRDLLDEVDRLALPEPDRQRLRTLVEEDIHTSLRFIQNAVTRSAGIID 325 NLQGFSKEI HAT DLL+ V L +P+ D+ +LR++V +DI +LRFIQNAVTRSAGIID Sbjct: 301 NLQGFSKEIGHATTDLLNAVQALPVPDADKAKLRSVVADDIQPALRFIQNAVTRSAGIID 360 Query: 326 AMLRLSRAGRVEYQPVMLDMHAIVTRIVAAMSGSIRAKSARVEIEADLPPAYGDPTSVEQ 385 +MLRLSRAGRVEYQPVMLDM+ I RIVAAMSGSIR K A VE+ +LPPA+GDPT++EQ Sbjct: 361 SMLRLSRAGRVEYQPVMLDMNVIAQRIVAAMSGSIRDKGAVVEVAPELPPAFGDPTAIEQ 420 Query: 386 VLGNLVGNAVNYLDPGRPGHITIGHVPQADSVVAAPSLVTYFVRDNGLGIPAAYMDKMFI 445 VLGNLVGNAVNYLDP RPG ITI H+ + P Y VRDNGLGIP AYM KMFI Sbjct: 421 VLGNLVGNAVNYLDPARPGRITIDHLREEGGAATGPETNVYVVRDNGLGIPPAYMHKMFI 480 Query: 446 AFQRLHGNAAKGEGIGLALVKRVVERHGGRIWVESVEGQGSCFYVALPARPP 497 AFQRLHGNAA GEGIGLALVKRVVERHGGRIWVES EGQGS FYVALPARPP Sbjct: 481 AFQRLHGNAAPGEGIGLALVKRVVERHGGRIWVESTEGQGSSFYVALPARPP 532 >gi|300311887|ref|YP_003775979.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 633 Score = 116 bits (291), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 84/279 (30%), Positives = 132/279 (47%), Gaps = 45/279 (16%) Query: 229 RARARE-LADVNSDLRQQ-------TQDNEMFIYSVSHDLRSPLVNLQGFSKEITHATRD 280 R RA E + +N+DL++Q ++ E F YSVSHDLR+PL + G++ + D Sbjct: 362 RKRAEEEIEALNADLKRQALHLHSVNKELESFSYSVSHDLRAPLRAISGYAMILEEDYAD 421 Query: 281 LLDEVDRLALPEPDRQRLRTLVEEDIHTSLRFIQNAVTRSAGIIDAMLRLSRAGRVEYQP 340 +DE R L+ I+ V + +I+ +L+L+++ E Sbjct: 422 AIDEKGR--------------------EQLQVIRRNVRKMDELINDLLKLAKSTTGELTL 461 Query: 341 VMLDMHAIVTRIVAAMSGSIRAKSARVEIEADLPPAYGDPTS---VEQVLGNLVGNAVNY 397 M +V +++A + + + D+PP G + + QV NL+ NAV + Sbjct: 462 ERFSMDDLVRQVIAGL------RRENPNVLFDVPPLEGAVANRGLITQVWENLLSNAVKF 515 Query: 398 LDPGRPGHITIGHVPQADSVVAAPSLVTYFVRDNGLGIPAAYMDKMFIAFQRLH-GNAAK 456 + + S+ P Y V DNG+G Y K+F FQRLH Sbjct: 516 SSKSEQPAVRV-------SMQVEPEEYIYGVHDNGVGFDMRYAHKLFGTFQRLHRQEEYA 568 Query: 457 GEGIGLALVKRVVERHGGRIWVESVEGQGSCFYVALPAR 495 G GIGLALV+R+V RH GR+W +S G+G+ FY +LP + Sbjct: 569 GTGIGLALVQRIVIRHTGRVWAQSKPGEGASFYFSLPRK 607 >gi|300310566|ref|YP_003774658.1| Hpt sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 723 Score = 87.8 bits (216), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 71/249 (28%), Positives = 121/249 (48%), Gaps = 36/249 (14%) Query: 252 FIYSVSHDLRSPLVNLQGFSKEITHATRDLLDEVDRLALPEPDRQRLRTLVEEDIHTSLR 311 F+ ++SH++R+P+ + G + + +TR LD + +E+ H L Sbjct: 90 FLSTMSHEIRTPMNAVLGMAG-LLRSTR--LDT---------QQAEFAKAIEDSAHALL- 136 Query: 312 FIQNAVTRSAGIIDAMLRLSR--AGRVEYQPVMLDMHAIVTRIVAAMSGSIRAKSARV-- 367 GI++ +L S+ G+++ V D+ +V A+S R K R+ Sbjct: 137 ----------GIVNDVLDFSKIDVGKMDIAVVEFDLAKVVEASAEALSFKAREKRVRLLA 186 Query: 368 EIEADLPP-AYGDPTSVEQVLGNLVGNAVNYLDPGRPGHITIGHVPQADSVVAAPSLVTY 426 +++ LPP GDP + Q+L NL+GNA+ + G + QA V + V + Sbjct: 187 HLDSRLPPRLVGDPGRIRQILLNLLGNALKFTHAGE-----VLLRVQALQVTSDHCRVRF 241 Query: 427 FVRDNGLGIPAAYMDKMFIAFQRLHGNAAK---GEGIGLALVKRVVERHGGRIWVESVEG 483 VRD+G+G+ + ++F F + + + G G+GL++ KR+VE GG I VES G Sbjct: 242 EVRDSGIGMDEDTLRRLFSPFAQADSSVTRQYGGTGLGLSICKRLVELMGGSIGVESALG 301 Query: 484 QGSCFYVAL 492 GSCF+ L Sbjct: 302 TGSCFWFEL 310 >gi|300313070|ref|YP_003777162.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 939 Score = 86.3 bits (212), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 72/251 (28%), Positives = 112/251 (44%), Gaps = 33/251 (13%) Query: 252 FIYSVSHDLRSPLVNLQGFSKEITHATRDLLDEVDRLALPEPDRQRLRTLVEEDIHTSLR 311 + ++SHDLR+PL +L G + D+L RL ++D R Sbjct: 711 LLSAISHDLRTPLTSLVG--------SADVLAANARL--------------DDDARAMAR 748 Query: 312 FIQNAVTRSAGIIDAMLRLSR--AGRVEYQPVMLDMHAIVTRIVAAMSGSIRAKSARVEI 369 I + G+++ +L ++R AG V + + + ++ ++ + I Sbjct: 749 TISEQSQKMVGLMNNLLDMARLQAGVVTLNRQWNALEELAGSALRLLAKALEGRQVDTRI 808 Query: 370 EADLPPAYGDPTSVEQVLGNLVGNAVNYLDPGRPGHITIGHVPQADSVVAAPSLVTYFVR 429 +LP D +E+VL NLV NA+ Y PG P I+ HV D AP V V Sbjct: 809 APELPLLRVDAVLLERVLANLVENAIKYSPPGTPIGIS-AHVEHGD---GAPQ-VKVCVM 863 Query: 430 DNGLGIPAAYMDKMFIAFQRLHGNAAK-GEGIGLALVKRVVERHGGRIWVESVE-GQGS- 486 D G G P F F R +A G G+GLA+ + ++E H GRIW+ E GQG+ Sbjct: 864 DQGRGFPPTMTQHAFEKFTRGDNESATPGVGLGLAICRAIIEAHQGRIWIAPQEAGQGAT 923 Query: 487 -CFYVALPARP 496 CF + + +P Sbjct: 924 VCFTLPVETQP 934 >gi|300314018|ref|YP_003778110.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 492 Score = 76.3 bits (186), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 131/491 (26%), Positives = 198/491 (40%), Gaps = 72/491 (14%) Query: 26 ALFKFQADAEDAEGWASHSLQVMNKAAELREPMLLEAARVRNAVLLDLPDTLSRGDLWAD 85 ALF + ++ A ++Q+ A L E + R ++LD P R + D Sbjct: 48 ALFTLDRMSANSRETARQAVQLTEAAQRLAERTVAMERSARQYLVLDDPAFHDRFNEARD 107 Query: 86 IEVKT-GELARLVADNASQSAAVRTVHESIASYRSWDDSARERLMRGQRDDLIRQLRDEQ 144 + GELA + + E + + D A L R D Q R Q Sbjct: 108 QARQALGELA---------DSLPKAPREMFGQWSVYVDEAAGVLDADSRKDKGEQARLFQ 158 Query: 145 GPHRLSTFLNLLDNFVAEERRLGAERSERLQAARRSQTWLLILAVLVSIVTAGLASLAF- 203 RL L + E RR R+ L AA Q +L V+ +IV A L + F Sbjct: 159 DFARLPA---LNERIALESRREVDRRNNALSAALEQQRQMLTAQVVGAIVLAVLLAFCFG 215 Query: 204 ------MRNISTRIGVLIGNARRLXSARARARARARELADVNSDLRQQTQDNEM----FI 253 M + IG L G+ R R A R L LRQ+ D E F+ Sbjct: 216 LWLSRPMARLEQAIGRL-GDNRFDQPIDVRGPADIRRLGQQLDWLRQRLADLEAEKSRFL 274 Query: 254 YSVSHDLRSPLVNL--------QGFSKEITHATRDLLDEVDRLALPEPDRQRLRTLVEED 305 VSH+L++PL L G + ++T + R E+ R+ RQ ++L + Sbjct: 275 RHVSHELKTPLAALCEGAALLDDGVAGQLTDSQR----EIARIL-----RQNTQSL-QTQ 324 Query: 306 IHTSLRFIQNAVTRSAGIIDAMLRLSRAGRVEYQPVMLDMHAIVTRIVAAMSGSIRAKSA 365 I LR+ N V+ A I PV +D+ A++ +++ A+ Sbjct: 325 IEDLLRY--NEVSFDAQRI--------------HPVPVDLRALLHKVIDDQRLQWLAREL 368 Query: 366 RVEIEADLPPAYGDPTSVEQVLGNLVGNAVNYLDPGRPGHITIGHVPQADSVVAAPSLVT 425 +VEIE DP + VL NL+ NAV + G G I + AP LV Sbjct: 369 KVEIEGAARTVVVDPEKMAIVLANLLSNAVRFSPQG--GFIRF-------LLSEAPGLVR 419 Query: 426 YFVRDNGLGIPAAYMDKMFIAF-QRLHGNAA--KGEGIGLALVKRVVERHGGRIWVESVE 482 D G G+ ++F F Q L+ A +G GIGL++V+ V+ H G++++ E Sbjct: 420 VECVDQGPGVAPTDAARIFEPFYQGLNQPTAARRGNGIGLSVVREYVQAHSGKVYLVPRE 479 Query: 483 GQGSCFYVALP 493 G G+ F + LP Sbjct: 480 G-GAHFRIELP 489 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,473,158 Number of extensions: 60353 Number of successful extensions: 400 Number of sequences better than 1.0e-08: 30 Number of HSP's gapped: 373 Number of HSP's successfully gapped: 30 Length of query: 499 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 410 Effective length of database: 1,198,455 Effective search space: 491366550 Effective search space used: 491366550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)