BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0034.p (416 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311804|ref|YP_003775896.1| iron-regulated membrane protein... 750 0.0 gi|300311746|ref|YP_003775838.1| iron-regulated membrane protein... 235 4e-63 gi|300310446|ref|YP_003774538.1| sulfite reductase, flavoprotein... 68 1e-12 >gi|300311804|ref|YP_003775896.1| iron-regulated membrane protein [Herbaspirillum seropedicae SmR1] Length = 416 Score = 750 bits (1936), Expect = 0.0, Method: Compositional matrix adjust. Identities = 363/414 (87%), Positives = 388/414 (93%) Query: 1 MRALLVLLHRWFGLAVAVFLFIAGVTGALISWDHELDAWLNPQLFHARSADRPDAGQPMP 60 MRA LVLLHRWFGLA AVFLFIAG+TGA+ISWDHELDAWLNPQLF ARSA +P AG PMP Sbjct: 1 MRATLVLLHRWFGLAAAVFLFIAGLTGAVISWDHELDAWLNPQLFDARSATQPGAGAPMP 60 Query: 61 GLELAQRVEAAEPKLRVTYVLTEAEPGHTISMMVEPRLDPATGKLPVLDYNQIAVDPVTG 120 GLELA+RVE AEP+LRVTYVLT+AEPGHTI MMVEPR+DPATGKL LDYNQIAVDP T Sbjct: 61 GLELARRVEQAEPRLRVTYVLTQAEPGHTIGMMVEPRVDPATGKLYTLDYNQIAVDPFTA 120 Query: 121 EIQGRRMWGAISLARENLLPFLYKLHYTMHLPDMGGIETGIWLMGIIGIVWALDCFIALY 180 EIQGRRMWGAISL+RENLLPFLYKLHYTMHLPD+GG+ETGI LMGIIGIVWALDC IALY Sbjct: 121 EIQGRRMWGAISLSRENLLPFLYKLHYTMHLPDLGGLETGILLMGIIGIVWALDCLIALY 180 Query: 181 LSFPNWRSWRKSLAFRWREGGHKLNFDLHRSGGVWIWGLLLVLAVTSVSMNLERQVMRPI 240 LSFPNWRSWRKSLAFRWR+GGHKLNFDLHRSGGVW+WGLLL+LAVTSVSMNLERQVMRP+ Sbjct: 181 LSFPNWRSWRKSLAFRWRDGGHKLNFDLHRSGGVWVWGLLLILAVTSVSMNLERQVMRPL 240 Query: 241 LQSVSTLTAGPFDTRTPIAPDKVPEPRISREEAVALGRAQGEKLGLTEPVGGVFYSGMFG 300 LQ++STLT PFDTRTP AP+KV EPRI+RE+AVALG+AQ K+GLTEPVGGVFYSGMFG Sbjct: 241 LQTLSTLTPSPFDTRTPTAPEKVAEPRITREQAVALGQAQARKMGLTEPVGGVFYSGMFG 300 Query: 301 VYGVGFFQPGNDHGDVGLGNAWLYFDAMSGAYEGGKIPGRGSAGDIFLQAQFPLHSGRIL 360 VYGVG+FQPGNDHGD+GLGNAWLYFDA SGAYEGGKIPGRGSAGDIFLQAQFPLHSGRIL Sbjct: 301 VYGVGYFQPGNDHGDIGLGNAWLYFDANSGAYEGGKIPGRGSAGDIFLQAQFPLHSGRIL 360 Query: 361 GLTGRVLISLMGVLVAMLSVTGVIIWVRKRRARLQQRERLAAQKTPAREGPVVE 414 GL GR+LIS MG LVAMLSVTGVIIWVRKR+ARLQQRER AAQKTPAREGPV E Sbjct: 361 GLPGRILISFMGALVAMLSVTGVIIWVRKRKARLQQRERTAAQKTPAREGPVAE 414 >gi|300311746|ref|YP_003775838.1| iron-regulated membrane protein [Herbaspirillum seropedicae SmR1] Length = 435 Score = 235 bits (600), Expect = 4e-63, Method: Compositional matrix adjust. Identities = 155/430 (36%), Positives = 229/430 (53%), Gaps = 28/430 (6%) Query: 2 RALLVLLHRWFGLAVAVFLFIAGVTGALISWDHELDAWLNPQLFHARSADRPDAGQPMPG 61 R +LVLLHRW GL +A FL + +TG+L+ +D ELDAWL PQL A+ A A M Sbjct: 19 RHVLVLLHRWTGLGMAFFLIVTSLTGSLLVFDDELDAWLAPQLQLAQPAPGQSAQAMMDP 78 Query: 62 LELAQRV-EAAEPKLRVTYVLTEAEPGHTISMMVEPRLDPATGKLPVLDYNQIAVDPVTG 120 L ++V +A P++ ++ ++ A PG T+S +P DPATGK L Y++I V+P TG Sbjct: 79 YLLREKVAQAMGPQVHISQLVLHARPGRTVSFQADPATDPATGKPYRLGYDEILVNPYTG 138 Query: 121 EIQGRRMWGAISLARENLLPFLYKLHYTMHLPDMGGIETGIWLMGIIGIVWALDCFIALY 180 +QG R ISL +L+PFL+KLH+++ LP G L+G +G++W LDCF+ + Sbjct: 139 VVQGVRDRDKISLKPADLMPFLFKLHHSLALPR----APGALLLGTVGLLWTLDCFVGMI 194 Query: 181 LSFPNWR----SWRKSLAFRWREGGHKLNFDLHRSGGVWIWGLLLVLAVTSVSMNLERQV 236 L++P R W+ + +WR G +++N DLHR+ G+W W +LL+ A +SV +NL Q+ Sbjct: 195 LTWPRARPFLVKWKPAWLIKWRAGFYRVNLDLHRAFGLWCWVMLLLFAWSSVMLNLRDQI 254 Query: 237 MRPILQSVSTLTAGPFDTR---TPIAPDKVPEPRISREEAVALGRAQGEKLGLTEPVGGV 293 P++ + + FDT P P + P S EA AL R + G G V Sbjct: 255 YLPVMSRILS-----FDTSWRPGPPLPQALSGPPQSWPEAHALARRTMD--GFARQRGLV 307 Query: 294 F-------YSGMFGVYGVGFFQPGNDHGDVGLGNAWLYFDAMSGAYEGGKIPGRGSAGDI 346 + GVY + DV GN L DA +G G +P G+ Sbjct: 308 IDFEEKLDFDRRRGVYIYLVHSNADLRADV--GNTGLRIDARTGKILGHWLPVGDRDGNT 365 Query: 347 FLQAQFPLHSGRILGLTGRVLISLMGVLVAMLSVTGVIIWVRKRRARLQQRERLAAQKTP 406 F LH G + GL R+L+ L+G V++L+VTGV++W +KR AR Q + P Sbjct: 366 FSNWLGALHMGHVFGLPWRILLVLVGGAVSLLTVTGVVVWWKKRAARRLQLRSPGDGRKP 425 Query: 407 AREGPVVEAR 416 + V +R Sbjct: 426 SASHSDVASR 435 >gi|300310446|ref|YP_003774538.1| sulfite reductase, flavoprotein component [Herbaspirillum seropedicae SmR1] Length = 850 Score = 68.2 bits (165), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 88/405 (21%), Positives = 166/405 (40%), Gaps = 63/405 (15%) Query: 8 LHRWFGLAVAVFLFIAGVTGALISWDHELDAWLNPQLFHARSADRPDAGQPMPGLELAQR 67 LH + G+ L GVTGAL+++ E+ L+P + H + + + ++ ++ Sbjct: 9 LHWFIGITAGTVLMTIGVTGALLAFREEIMDLLSPGVMHVQVRQQAT----LTPQQVLEK 64 Query: 68 VEAAEPKLRVTYVLTEAEPGHTISMMVEPRLDPATGKLPVLD-YNQIAVDPVTGEIQGRR 126 + EP+ R+T + AEPG ++ ++ P G+ +D Y + PVTG Sbjct: 65 LRQEEPQRRITTMTLSAEPGDSVRVVFAPPPGERRGQARYVDPYTGALLPPVTG------ 118 Query: 127 MWGAISLARENLLPFLYKLHYTMHLPDMGGIETGIWLMGIIGIVWALDCFIALYLSFP-- 184 F + H + LP E G ++G + + + LYL +P Sbjct: 119 ---------MEFFEFTERFHRWLLLP----TEIGKIVLGTLALCLLMLALSGLYLRWPRR 165 Query: 185 --NWRSWRKSLAFRWREGGHKLNFDLHRSGGVWIWGLLLVLAVTSVSMNLE--------- 233 + R+W L ++ G L ++LH G W + ++ A T L+ Sbjct: 166 ALSLRAW---LRLDFQLQGRSLLWNLHSVIGTWSLVMYVIFATTGAYWALDWFKDGVNTL 222 Query: 234 --RQVMRPILQSVSTLTAGPFDTRTPIAPDKVPEPRISREEAVALGRA---QGEKLGLTE 288 P+ + GP + +TP+A D +++ L +A Q +L L E Sbjct: 223 AGETRRAPMARGEGKKKDGP-NNQTPVALD------LTQAWNAFLTKAPDYQSAQLRLPE 275 Query: 289 PVGGVFYSGMFGVYGVGFFQPGNDHGDVGLGNAWLYFDAMSGAYEGGKIPGRGSAGDIFL 348 G + F G+D + + SG + + S G F+ Sbjct: 276 Q----------GTQPLQIFFLGHD-APHERARSRMVVLPQSGEIKQLERYEDKSTGGRFI 324 Query: 349 QAQFPLHSGRILGLTGRVLISLMGVLVAMLSVTGVIIWVRKRRAR 393 A +PLH G GL GR+++++ +++ + +TG ++++ +RR + Sbjct: 325 AAIYPLHMGTYFGLPGRIIMAIASLMMPLFGITGWMLYLDRRRKK 369 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.141 0.446 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,413,210 Number of extensions: 64426 Number of successful extensions: 220 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 216 Number of HSP's successfully gapped: 3 Length of query: 416 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 328 Effective length of database: 1,203,190 Effective search space: 394646320 Effective search space used: 394646320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)