BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0032.p (153 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311802|ref|YP_003775894.1| extradiol dioxygenase [Herbaspi... 251 2e-68 >gi|300311802|ref|YP_003775894.1| extradiol dioxygenase [Herbaspirillum seropedicae SmR1] Length = 138 Score = 251 bits (641), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 119/138 (86%), Positives = 126/138 (91%) Query: 16 MPSIFHLAYHVRDLDQSRRFYCDLLGCAEGRSTETWVDIDFFGHQLSLHLGEPFAVSNTG 75 M SIFHLAYHVRDLD +RRFYCDLLGC EGRST+TWVDIDFFGHQLSLHLGEPFAVSNTG Sbjct: 1 MSSIFHLAYHVRDLDAARRFYCDLLGCQEGRSTDTWVDIDFFGHQLSLHLGEPFAVSNTG 60 Query: 76 HVGHHLVPMPHLGVILQLDEWQQLADRLSASGEVQFVLAPQIRFAGEPGEQATMFFLDPS 135 VGHH+VPMPHLGVIL + +WQ LADRLSAS +VQFVLAPQIRFAGEPGEQATMFF DPS Sbjct: 61 KVGHHMVPMPHLGVILPMPQWQALADRLSASAQVQFVLAPQIRFAGEPGEQATMFFCDPS 120 Query: 136 GNPIEVKGFRDLATVYAK 153 GNPIEVKGF DL VYA+ Sbjct: 121 GNPIEVKGFADLQQVYAR 138 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.142 0.449 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 558,311 Number of extensions: 23437 Number of successful extensions: 44 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 44 Number of HSP's successfully gapped: 1 Length of query: 153 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 76 Effective length of database: 1,255,275 Effective search space: 95400900 Effective search space used: 95400900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 126 (53.1 bits)