BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0030.p (476 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311800|ref|YP_003775892.1| two component heavy metal senso... 795 0.0 gi|300309362|ref|YP_003773454.1| transmembrane sensory transduct... 200 2e-52 gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance ... 180 3e-46 gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine ... 100 3e-22 gi|300311751|ref|YP_003775843.1| signal transduction histidine k... 96 6e-21 >gi|300311800|ref|YP_003775892.1| two component heavy metal sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 467 Score = 795 bits (2054), Expect = 0.0, Method: Compositional matrix adjust. Identities = 399/465 (85%), Positives = 420/465 (90%), Gaps = 1/465 (0%) Query: 12 RRLPLRQSITARLVLMSAASALLIFAVAGVVLYTVLKGELLRHERDALFTTLNDISYQIE 71 R LPLRQSITARLV MSAASALLIFA+ G+VLYTVLKGELLRHERD L TTLNDISYQI Sbjct: 4 RWLPLRQSITARLVWMSAASALLIFALTGMVLYTVLKGELLRHERDGLVTTLNDISYQIG 63 Query: 72 RAGNTERWARVQLKLSTLSQADQHVFFWIASDDPRYTYGDLTRLAPSAINHADGKQVGRL 131 RAGNTERW RVQ KL+TLSQADQHV FWI+S D R+TYGDL+RLAP AI +G VGRL Sbjct: 64 RAGNTERWGRVQAKLATLSQADQHVSFWISSSDARFTYGDLSRLAPQAIRADEGNSVGRL 123 Query: 132 HLPGRAYPLHIMTRTLPAFEDRPEVRVTVGVDGAPYFEMRRTFLLATSISSLLGIVLAVI 191 HLPGR YPLHIMTRTLPAFE+RP V VTVGVDG PYFEMRRTFLLATS+ SLLGIVLAVI Sbjct: 124 HLPGRRYPLHIMTRTLPAFEERPAVDVTVGVDGEPYFEMRRTFLLATSVFSLLGIVLAVI 183 Query: 192 SSYWIARAGLEGLKKGGAARAQSFSPRKLSARMPVEALPEELSVLADAFNGALERIEQAY 251 SSYWIARAGL L++ + RAQSFSPRKLSARMPVEA+P+ELSVLA AFNGAL+RIE AY Sbjct: 184 SSYWIARAGLAPLRQM-SDRAQSFSPRKLSARMPVEAMPQELSVLALAFNGALDRIEDAY 242 Query: 252 TQLEAFNADVAHELRTPLANLIGETQVALARERSAGEFKDVLQSNLEELDRIRSIVNDML 311 TQLEAFNADVAHELRTPLANLIGETQVALARERSAGEFKDVLQSNLEELDRIRSIVNDML Sbjct: 243 TQLEAFNADVAHELRTPLANLIGETQVALARERSAGEFKDVLQSNLEELDRIRSIVNDML 302 Query: 312 FLARSDQGEAATARVPVRVAEAVQKTVDFFEFVLDDTGMSITVAGDLEATATMETALFKR 371 FLARSDQGEAATARVP RVA+ VQKTVDFFEFVLDD+GM I VAGD EATA METALFKR Sbjct: 303 FLARSDQGEAATARVPARVADEVQKTVDFFEFVLDDSGMRIEVAGDTEATAMMETALFKR 362 Query: 372 ALTNLIQNAIQHSPQGAQLRIDISGGEEMVRVAVTNPGPAIDEQHLPRLFDRFYRVDSAR 431 ALTNLIQNAIQHSP+GA LRIDI+ EE VRVAV+NPG IDE HLPRLFDRFYRVDSAR Sbjct: 363 ALTNLIQNAIQHSPEGALLRIDIACQEETVRVAVSNPGKPIDEHHLPRLFDRFYRVDSAR 422 Query: 432 QDINGSHGHGLGLAIVKAVARMHGGRAFASSSGGHNTIGFTIARQ 476 QDINGSHGHGLGLAIVKAVARMHGGRAFASS G N+IGFTIARQ Sbjct: 423 QDINGSHGHGLGLAIVKAVARMHGGRAFASSKEGVNSIGFTIARQ 467 >gi|300309362|ref|YP_003773454.1| transmembrane sensory transduction histidine kinase for cobalt/zinc/ cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 467 Score = 200 bits (508), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 154/469 (32%), Positives = 253/469 (53%), Gaps = 38/469 (8%) Query: 19 SITARLVLMSAASALLIFAVAGVVLYTVLKGELLRHERDALFTTLNDISYQIERAGNTER 78 S++ RLV + A++I A G L L +L R + + L I++ +E A + E Sbjct: 8 SLSLRLVALFGLLAMVILAALGTYLSHSLHVQLERRDDEELAGKCRSINHLVEHAASLED 67 Query: 79 -WARVQLKLSTLSQADQHVF--------FWIASDDPRYTYG--DLTRLAPSAINHADGKQ 127 AR L L TL+ DQ + + S DP T DL+++ A + + Sbjct: 68 ILARRHLVLDTLTGHDQLLVRIARADGSTLLESHDPTRTPPGIDLSQVDIRAWDSIQSWK 127 Query: 128 VGRLHLPGRAYPLHIMTRTLPAFEDRPEVRVTVGVDGAPYFEMRRTFLLATSISSL---- 183 +G L R +TR+ E V + V A R LLA S + Sbjct: 128 LGDLR--ARYVASKPVTRS-------GEQAVVLVVRTAS----DRMLLLAEYRSDVWWAA 174 Query: 184 -LGIVLAVISSYWIARAGLEGLKKGGAARAQSFSPRKLSARMPVEALPEELSVLADAFNG 242 +G +LA++ SY++ R+GL L+ AA+ ++ S +L R+ ++A P EL + ++FN Sbjct: 175 GVGALLAILMSYFLVRSGLRPLRTM-AAQTRAVSANRLDTRLDLQAAPRELHEIVESFNE 233 Query: 243 ALERIEQAYTQLEAFNADVAHELRTPLANLIGETQVALARERSAGEFKDVLQSNLEELDR 302 L+R+ +++ QL F+AD+AH++RTPL NL+ +TQVAL + R E++ +L SN+EE +R Sbjct: 234 MLDRLHRSFQQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQALLSSNIEEYER 293 Query: 303 IRSIVNDMLFLARSDQGEAATARVPVRVAEAVQKTVDFFEFVLDDTGMSITV--AGDLEA 360 + ++ MLFLAR++ + VA+ + + D+FE + D+ + + V AG L+A Sbjct: 294 LSRMMESMLFLARAENAHVVLQWQELDVAQELTRIADYFEGIADEAQVRLVVQGAGRLQA 353 Query: 361 TATMETALFKRALTNLIQNAIQHSPQGAQLRIDISGGEEMVRVAVTNPGPAIDEQHLPRL 420 AT L +RA+ NL+ NAI+++P +R+ + + + ++V+NPG I + L RL Sbjct: 354 DAT----LLRRAVGNLVANAIRYTPADGVIRLQVDAAGDGMEISVSNPGAGIPAEQLERL 409 Query: 421 FDRFYRVDSARQDINGSHGHGLGLAIVKAVARMHGGRAFASSSGGHNTI 469 FDRFYR D +R + GLGLAIV+++ +HGG+A +SS G T+ Sbjct: 410 FDRFYRADPSRS--SAGGSSGLGLAIVRSIMMLHGGQASVTSSPGQLTV 456 >gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 180 bits (456), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 101/250 (40%), Positives = 151/250 (60%), Gaps = 8/250 (3%) Query: 211 RAQSFSPRKLSARMPVEALPEELSVLADAFNGALERIEQAYTQLEAFNADVAHELRTPLA 270 RA +P LSAR+ E +P EL +A +FN L+R+E Y L F+ D+AHE+RTP+ Sbjct: 208 RAAQITPDHLSARLDQEGMPAELRRVALSFNAMLDRLEAGYAHLAQFSGDLAHEIRTPIN 267 Query: 271 NLIGETQVALARERSAGEFKDVLQSNLEELDRIRSIVNDMLFLARSDQGEAATARVPVRV 330 L+G++QVAL + RS E++ VL+SN+EEL R+ IV ++LFLA++D A R + + Sbjct: 268 VLMGQSQVALGQRRSPEEYEQVLESNVEELQRLSRIVENILFLAQADHAALAVERNALDL 327 Query: 331 AEAVQKTVDFFEFVLDDTGMSITV--AGDLEATATMETALFKRALTNLIQNAIQHSPQGA 388 AE + + D+FE + D+ GM V AG +A M ++R ++NL+ NA+++ + Sbjct: 328 AEELGRITDYFEGIADERGMRFDVQAAGQCQANLIM----WRRVVSNLVINAVRYGRAES 383 Query: 389 QLRIDISGGEEMVRVAVTNPGPAIDEQHLPRLFDRFYRVDSARQDINGSHGHGLGLAIVK 448 +RI E R+ V N + Q + R+FDRFYR D +R S +GLGLAIVK Sbjct: 384 TIRILAESDGEGSRIMVENRSEDVTAQAMARMFDRFYRGDRSRSAFTES--NGLGLAIVK 441 Query: 449 AVARMHGGRA 458 A+ +HGG A Sbjct: 442 AIMALHGGTA 451 >gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 444 Score = 100 bits (248), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 89/281 (31%), Positives = 137/281 (48%), Gaps = 39/281 (13%) Query: 195 WIARA-GLEGLKKGGAARAQSFSPRKLSARMPVE--ALPEELSVLADAFNGALERIEQAY 251 W+A + GL ++K A + R +++ P+ +P+E+ L A N L R++ A Sbjct: 158 WVAVSRGLAPVRKAAA----EVAARDMNSLQPLADVGMPQEIQPLTHALNDLLARLKHAT 213 Query: 252 TQLEAFNADVAHELRTPLANLIGETQV---ALARERSAGEFKDVLQSNLEELDRIRSIVN 308 AF AD AHELRTPL L +TQ+ A+ E F D L++ LE R +V+ Sbjct: 214 ETQRAFVADAAHELRTPLTALRLQTQLAERAVDEEERRAAFAD-LKNGLE---RTTHLVH 269 Query: 309 DMLFLARSDQGEAATARVPVRV----------AEAVQKTVDFFEFVLDDTGMSITVAGDL 358 +L LAR + + +V +R + A +D D G + V G+ Sbjct: 270 QLLTLARQEPDAMSFTQVDLRALLGSVVGDLSSVAEHGGIDLGLSDQGDDGRPLRVTGNA 329 Query: 359 EATATMETALFKRALTNLIQNAIQHSPQGA--QLRIDISGGEEMVRVAVTNPGPAIDEQH 416 +A + NL+ NA++++P G + +D+S V V + GP I E Sbjct: 330 DALRIL--------FNNLVDNALRYTPAGGVVDVGVDLSTA-GYCTVVVQDSGPGIAESE 380 Query: 417 LPRLFDRFYRVDSARQDINGSH---GHGLGLAIVKAVARMH 454 LPR+FDRFYR + Q+ +GS G GLGLAIV+ +A +H Sbjct: 381 LPRVFDRFYRAQHS-QEGSGSRAAFGSGLGLAIVRQIADLH 420 >gi|300311751|ref|YP_003775843.1| signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 446 Score = 95.9 bits (237), Expect = 6e-21, Method: Compositional matrix adjust. Identities = 69/227 (30%), Positives = 109/227 (48%), Gaps = 8/227 (3%) Query: 229 LPEELSVLADAFNGALERIEQAYTQLEAFNADVAHELRTPLANLIGETQVALARERSAGE 288 LP E+ A NG L R+E F AD AHELRTPL L + + L R + Sbjct: 203 LPREVQAFVAAINGLLARVEAGMAAQRRFVADAAHELRTPLTALSLQAE-RLGRAPMSET 261 Query: 289 FKDVLQSNLEELDRIRSIVNDMLFLARSDQGEAATARVPVRVAEAVQKTVDFFEFVLDDT 348 ++ L L R R +V +L LAR+ Q A A P + + +++ ++ + Sbjct: 262 ARERLTRLETGLQRARQLVTQLLALARA-QTPAGAALQPCSLHQVLREVIEELLPLAQCK 320 Query: 349 GMSITVAGDLEATATMETALFKRALTNLIQNAIQHSPQGAQLRIDISGGEEMVRVAVTNP 408 + + + G+ +A + + L NLI NAI ++P G + + + + V + + + Sbjct: 321 QIDLGLDGERDACVLTQPQELAQLLRNLIDNAIAYTPAGGAVSLRMERHAQWVALEICDT 380 Query: 409 GPAIDEQHLPRLFDRFYRVDSARQDINGSHGHGLGLAIVKAVARMHG 455 GP I R+F FYR+D + Q G GLGLAIV+++AR G Sbjct: 381 GPGIAASERERVFQPFYRIDPSGQ------GSGLGLAIVQSIARRLG 421 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,336,639 Number of extensions: 54958 Number of successful extensions: 374 Number of sequences better than 1.0e-08: 38 Number of HSP's gapped: 315 Number of HSP's successfully gapped: 38 Length of query: 476 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 387 Effective length of database: 1,198,455 Effective search space: 463802085 Effective search space used: 463802085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)