BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0029.p (230 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311799|ref|YP_003775891.1| two component heavy metal respo... 441 e-125 gi|300312356|ref|YP_003776448.1| cobalt/zinc/cadmium resistance ... 272 2e-74 gi|300309361|ref|YP_003773453.1| response regulator for cobalt/z... 263 1e-71 gi|300309981|ref|YP_003774073.1| two component response regulato... 155 2e-39 gi|300313818|ref|YP_003777910.1| two component transcription reg... 155 4e-39 >gi|300311799|ref|YP_003775891.1| two component heavy metal response transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 230 Score = 441 bits (1133), Expect = e-125, Method: Compositional matrix adjust. Identities = 214/230 (93%), Positives = 225/230 (97%) Query: 1 MKILIVEDEQKMSEYLCKGLTEQGCAVDVASNGIDGQHLAIQHDYDVIVLDVMLPGLDGF 60 MKILIVEDEQKMSEYLCKGLTEQGCAVD+A+NG+DGQHLAI+HDYDVIVLDVMLPGLDGF Sbjct: 1 MKILIVEDEQKMSEYLCKGLTEQGCAVDLAANGVDGQHLAIEHDYDVIVLDVMLPGLDGF 60 Query: 61 AVLRSLRTVKQTPVIMLTARDSVEDRIKGLHDGADDYLIKPFSFLELLARLQALTRRGRV 120 +VLR+LR VK+TPVIMLTARDSVEDRIKGLHDGADDYLIKPFSFLELLARLQALTRRGR Sbjct: 61 SVLRALRKVKETPVIMLTARDSVEDRIKGLHDGADDYLIKPFSFLELLARLQALTRRGRA 120 Query: 121 QEAPQLRIGDLQIDLISRKAFRGGLRIDLTAKEFALLAVMARRQGEILSKTAIAELVWDM 180 QE QLRIGDLQIDL+SRKAFRGGLRIDLTAKEFALLAVMARRQGEILSKTAIAELVWDM Sbjct: 121 QEPAQLRIGDLQIDLLSRKAFRGGLRIDLTAKEFALLAVMARRQGEILSKTAIAELVWDM 180 Query: 181 NFDSNTNVVEVAIKRLRAKIDAPFEQKLLHTVRGMGYVLEPRATSDAGDE 230 NFDSNTNVVEVAIKRLRAKIDAPFEQKLLHTVRGMGYVLEPRAT+D+ D+ Sbjct: 181 NFDSNTNVVEVAIKRLRAKIDAPFEQKLLHTVRGMGYVLEPRATADSSDD 230 >gi|300312356|ref|YP_003776448.1| cobalt/zinc/cadmium resistance two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 223 Score = 272 bits (695), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 132/223 (59%), Positives = 171/223 (76%), Gaps = 2/223 (0%) Query: 1 MKILIVEDEQKMSEYLCKGLTEQGCAVDVASNGIDGQHLAIQHDYDVIVLDVMLPGLDGF 60 M IL++ED+ K +YL KGL E G AVD+A NG DG H+A++ DYD++VLDVMLPGLDG+ Sbjct: 1 MAILVIEDDPKTGDYLRKGLRESGYAVDLARNGADGLHMALEQDYDLVVLDVMLPGLDGW 60 Query: 61 AVLRSLRTVKQTPVIMLTARDSVEDRIKGLHDGADDYLIKPFSFLELLARLQALTRRGRV 120 V+ +LR+ + PV+ LTARD V+DRI+GL GADDYL+KPFSF EL+ R++ L RRG Sbjct: 61 QVMSALRSKRDLPVLFLTARDHVDDRIRGLQLGADDYLVKPFSFTELVLRIRTLLRRGVT 120 Query: 121 QEAPQLRIGDLQIDLISRKAFRGGLRIDLTAKEFALLAVMARRQGEILSKTAIAELVWDM 180 +EA I DLQ+D + K R G+ I LT KEF LL ++ +RQGE LS++ IA +WDM Sbjct: 121 REAEVYEIADLQLDHVRHKVTRQGVNIALTNKEFMLLHLLIKRQGEALSRSVIASQIWDM 180 Query: 181 NFDSNTNVVEVAIKRLRAKIDAPFEQKLLHTVRGMGYVL--EP 221 NFDS+TNVV+VAIKRLRAKIDAPF++KL+HTVR +GY+ EP Sbjct: 181 NFDSDTNVVDVAIKRLRAKIDAPFDKKLIHTVRSVGYMFSEEP 223 >gi|300309361|ref|YP_003773453.1| response regulator for cobalt/zinc/cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 235 Score = 263 bits (672), Expect = 1e-71, Method: Compositional matrix adjust. Identities = 130/219 (59%), Positives = 163/219 (74%) Query: 1 MKILIVEDEQKMSEYLCKGLTEQGCAVDVASNGIDGQHLAIQHDYDVIVLDVMLPGLDGF 60 M IL+VEDE K +YL +GLTE G VD G+DG H A DY +I+LDVMLPG++G+ Sbjct: 1 MHILLVEDEPKSGDYLRRGLTESGFVVDWVQTGVDGLHNARTLDYKLIILDVMLPGMNGW 60 Query: 61 AVLRSLRTVKQTPVIMLTARDSVEDRIKGLHDGADDYLIKPFSFLELLARLQALTRRGRV 120 +LR LR TPV+ LTARD VEDR+KGL GADDYL+KPF++ ELLAR++ L RRG Sbjct: 61 DILRELRRTHDTPVLFLTARDEVEDRVKGLELGADDYLVKPFAYTELLARVRTLLRRGPS 120 Query: 121 QEAPQLRIGDLQIDLISRKAFRGGLRIDLTAKEFALLAVMARRQGEILSKTAIAELVWDM 180 +E + D+ +D++ RK R G RIDLTAKEFALL ++ RR GE+LS+ IA VWDM Sbjct: 121 REVDLIEYDDVVLDVLRRKVTRAGQRIDLTAKEFALLHMLIRRPGEVLSRPQIASQVWDM 180 Query: 181 NFDSNTNVVEVAIKRLRAKIDAPFEQKLLHTVRGMGYVL 219 NFDS+TNVV+VA++RLRAK+D FE+KLLHTV GMGYV Sbjct: 181 NFDSDTNVVDVAVRRLRAKLDEGFEKKLLHTVWGMGYVF 219 >gi|300309981|ref|YP_003774073.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 237 Score = 155 bits (392), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 87/221 (39%), Positives = 134/221 (60%), Gaps = 3/221 (1%) Query: 1 MKILIVEDEQKMSEYLCKGLTEQGCAVDVASNGIDGQHLAIQHDYDVIVLDVMLPGLDGF 60 M+IL+ ED+ +++ L + L G A D NG++ D+D+++LD+ LP + G Sbjct: 1 MRILLAEDDSVLADGLTRSLRHSGYAADCVKNGVEADSALSTQDFDLLILDLGLPKMSGL 60 Query: 61 AVLRSLRTVK-QTPVIMLTARDSVEDRIKGLHDGADDYLIKPFSFLELLARLQALTRRGR 119 VLR LR + PV++LTA DSVE R++GL GADDY+ KPF+ EL AR++ALTRRG Sbjct: 61 EVLRRLRARNSRLPVLILTAADSVEQRVQGLDLGADDYMAKPFALSELEARVRALTRRGA 120 Query: 120 VQEAPQLRIGDLQIDLISRKAFRGGLRIDLTAKEFALLAVMARRQGEILSKTAIAELVWD 179 +R G L D + R A+ G ++L+A+E LL V+ +R G ++SK + + + + Sbjct: 121 GGGPTVIRHGPLSYDQVGRIAYIGEQMLELSARELGLLEVLLQRTGRLVSKEQLVDHLCE 180 Query: 180 MNFDSNTNVVEVAIKRLRAKIDAPFEQKLLHTVRGMGYVLE 220 + + N +EV + RLR KI+ + + TVRG+GY LE Sbjct: 181 WGEEVSNNAIEVYVHRLRKKIEVGGVR--IATVRGLGYCLE 219 >gi|300313818|ref|YP_003777910.1| two component transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 224 Score = 155 bits (391), Expect = 4e-39, Method: Compositional matrix adjust. Identities = 84/222 (37%), Positives = 135/222 (60%), Gaps = 2/222 (0%) Query: 1 MKILIVEDEQKMSEYLCKGLTEQGCAVDVASNGIDGQHLAIQHDYDVIVLDVMLPGLDGF 60 M+IL+VED ++S +L K + + V+ A NG D L + DY +++LD+ LP +DG Sbjct: 1 MRILLVEDHIELSRWLAKAMRDAHLTVECAHNGADADSLLLTQDYALVILDLTLPRMDGM 60 Query: 61 AVLRSLRTV-KQTPVIMLTARDSVEDRIKGLHDGADDYLIKPFSFLELLARLQALTRRGR 119 VL+ +R +TPV++LTAR + DR+ GL+ GADDYL KPF EL AR++AL RR + Sbjct: 61 EVLKRMRARGNRTPVLILTARGGLADRVSGLNMGADDYLAKPFELEELEARVKALLRRSQ 120 Query: 120 VQEAPQLRIGDLQIDLISRKAFRGGLRIDLTAKEFALLAVMARRQGEILSKTAIAELVWD 179 EA + G L D +SR GG + LT +E A+L + R G + K + + V+ Sbjct: 121 NNEAVTVSCGALSFDTVSRTFTYGGELLALTPREHAVLEALIVRHGHTVPKEKLFQQVFT 180 Query: 180 MNFDSNTNVVEVAIKRLRAKIDAPFEQKL-LHTVRGMGYVLE 220 + +++ + +E+ I RLR K++ ++ + T+RG+GY+L+ Sbjct: 181 LEDNASMDAIEIYIHRLRKKLEHEGPGRVGITTLRGLGYLLQ 222 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.139 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 666,503 Number of extensions: 25945 Number of successful extensions: 221 Number of sequences better than 1.0e-08: 39 Number of HSP's gapped: 203 Number of HSP's successfully gapped: 39 Length of query: 230 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 148 Effective length of database: 1,231,600 Effective search space: 182276800 Effective search space used: 182276800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)