BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0026.p (416 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprot... 473 e-134 gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspiri... 327 1e-90 gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 221 1e-58 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 205 6e-54 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 150 2e-37 >gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprotein [Herbaspirillum seropedicae SmR1] Length = 510 Score = 473 bits (1217), Expect = e-134, Method: Compositional matrix adjust. Identities = 275/316 (87%), Positives = 294/316 (93%) Query: 91 AIQATLAQNYLQLRVTDLQKDLYRRTVEAYTRSLQLTTHQYEAGTALRSDVAQAETQLRS 150 +IQATLAQ+YLQLRVTDLQKDLYRRTV AYTRSLQL+THQYEAGTALRSDVAQAETQLR+ Sbjct: 195 SIQATLAQDYLQLRVTDLQKDLYRRTVAAYTRSLQLSTHQYEAGTALRSDVAQAETQLRA 254 Query: 151 AQAQLIDLDATRNQLEHAIAMLIGKAPAAFSLAPLPAAAATDSGMDANALRLQASLPQIP 210 AQAQLIDLD TRNQLEHAIAMLIGKAPA FSL LP AAA+DS +DANA RLQ SLPQIP Sbjct: 255 AQAQLIDLDDTRNQLEHAIAMLIGKAPAQFSLPALPPAAASDSAIDANAARLQGSLPQIP 314 Query: 211 AGLPSDLLERRPDIANAERLAAAANANIGVARAAYFPTLTLSTTGGYNSASFADLFNTPS 270 GLPS+LLERRPDIANAERLAAAANANIGVARAAYFPTLTLS +GGYNS +FA+LFNTPS Sbjct: 315 VGLPSELLERRPDIANAERLAAAANANIGVARAAYFPTLTLSASGGYNSLAFANLFNTPS 374 Query: 271 RVWSLGAALAETLFDGGARTARNDAAMAAYDAAAAQYKQTVLAGLQDVEDNLSTLRVLDQ 330 RVWSLG+ALAE+LFDGGAR+ARNDAA+AAYDAA AQYKQTVL GLQ+VED LSTLRVLDQ Sbjct: 375 RVWSLGSALAESLFDGGARSARNDAAVAAYDAAVAQYKQTVLGGLQEVEDKLSTLRVLDQ 434 Query: 331 ESAAQAQAVRSAQLAERLAMSQYQAGTVTYLSVVTTQATSLTNQRSAVTLLGRQLVASVA 390 ES QAQAV+SAQLAERLAM QY+AGTVTYLSVVTTQA SLTNQR+AVTLLGRQLVASVA Sbjct: 435 ESTVQAQAVQSAQLAERLAMRQYEAGTVTYLSVVTTQAASLTNQRNAVTLLGRQLVASVA 494 Query: 391 LIKATGGGWQVGQTDP 406 LIKATGGGWQ G+ P Sbjct: 495 LIKATGGGWQAGRDAP 510 Score = 143 bits (360), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 62/73 (84%), Positives = 67/73 (91%) Query: 1 MTVLVLAALLGGCAVGPDYVRPAMDLPQAYKEQGPWKVAAPRQADDHQRWWEAYGDPILN 60 MT LVLAALLGGCAVGPDY RPA+DLPQAYKE GPWK+AAPRQ DDHQ WWEAYGDP+LN Sbjct: 35 MTALVLAALLGGCAVGPDYARPALDLPQAYKETGPWKLAAPRQIDDHQPWWEAYGDPVLN 94 Query: 61 DLVNQANAANQTI 73 L++QANAANQTI Sbjct: 95 ALMDQANAANQTI 107 >gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 507 Score = 327 bits (838), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 172/309 (55%), Positives = 221/309 (71%), Gaps = 19/309 (6%) Query: 93 QATLAQNYLQLRVTDLQKDLYRRTVEAYTRSLQLTTHQYEAGTALRSDVAQAETQLRSAQ 152 QA +AQNYLQLRV D Q+ L TV+AY RSLQ+ +QY AG +SDV QA+TQL+SAQ Sbjct: 187 QAQVAQNYLQLRVLDAQQQLLEETVQAYERSLQVAQNQYAAGMVAKSDVIQAQTQLKSAQ 246 Query: 153 AQLIDLDATRNQLEHAIAMLIGKAPAAFSL--APLPAAAATDSGMDANALRLQASLPQIP 210 A +D TR Q+EHAIA+L+GK PA F+L APL A A P +P Sbjct: 247 ASALDNGVTRAQVEHAIALLVGKTPAQFALPRAPLAAVA-----------------PPVP 289 Query: 211 AGLPSDLLERRPDIANAERLAAAANANIGVARAAYFPTLTLSTTGGYNSASFADLFNTPS 270 G+PS LLERRPD+A AER A+ANA IGVA+AAYFP+LTLS +GGY S++FAD F P Sbjct: 290 QGVPSMLLERRPDVAAAERRVASANAKIGVAKAAYFPSLTLSASGGYQSSTFADWFTLPG 349 Query: 271 RVWSLGAALAETLFDGGARTARNDAAMAAYDAAAAQYKQTVLAGLQDVEDNLSTLRVLDQ 330 RVWSLG LA+T+FDGGAR A D A+A YD A YKQTVL Q+VEDNL+ LR+L+Q Sbjct: 350 RVWSLGPQLAQTIFDGGARAAATDQAIATYDGTVATYKQTVLTAFQEVEDNLAALRILEQ 409 Query: 331 ESAAQAQAVRSAQLAERLAMSQYQAGTVTYLSVVTTQATSLTNQRSAVTLLGRQLVASVA 390 E+A Q +A+++A+ A L +QY+AGTV+Y++V+T QAT+L+ + S++ +L R++ ASV Sbjct: 410 EAAVQGEALQAARQAVTLVNNQYKAGTVSYVNVITAQATALSAELSSLNILNRRMAASVL 469 Query: 391 LIKATGGGW 399 L KA GGGW Sbjct: 470 LAKALGGGW 478 Score = 80.9 bits (198), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 40/86 (46%), Positives = 51/86 (59%), Gaps = 1/86 (1%) Query: 1 MTVLVLAALLGGCAVGPDYVRPAMDL-PQAYKEQGPWKVAAPRQADDHQRWWEAYGDPIL 59 + +LV + L C+VGPDYVRP MD P +KE WK A PR +WWE +GDP L Sbjct: 13 LAMLVASLALAACSVGPDYVRPQMDTGPAIFKEGQNWKPAQPRDLVADGKWWEMFGDPQL 72 Query: 60 NDLVNQANAANQTIQQAAAQYRQAQA 85 N LV Q + +NQ + A A +R A A Sbjct: 73 NALVAQVDISNQNLALAEANFRSAVA 98 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 221 bits (562), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 145/330 (43%), Positives = 200/330 (60%), Gaps = 19/330 (5%) Query: 76 AAAQYRQAQATAAVGAIQATLAQNYLQLRVTDLQKDLYRRTVEAYTRSLQLTTHQYEAGT 135 A AQ +A + + A+QA +AQ Y +R D LYR TV+ SL+L +++ G Sbjct: 181 ADAQRSEALFHSVLLALQADVAQQYFLVRELDAAAALYRGTVDLRQESLRLVQRRFDEGD 240 Query: 136 ALRSDVAQAETQLRSAQAQLIDLDATRNQLEHAIAMLIGKAPAAFSLAPLPAAAATDSGM 195 DVA+A +L SAQ++ + + R EHA+A+L+G+ PA FSL P P Sbjct: 241 ISEVDVARARAELASAQSEALGIARQRATAEHALAILLGRTPAEFSLPPQPLT------- 293 Query: 196 DANALRLQASLPQIPAGLPSDLLERRPDIANAERLAAAANANIGVARAAYFPTLTLSTTG 255 RL + +P GLPS LLERRPDIA AER AAANA +GVA++A+FP+L L+ Sbjct: 294 -----RLAVT---VPPGLPSALLERRPDIAAAERAMAAANARVGVAKSAFFPSLNLTGGL 345 Query: 256 GYNSASFADLFNTPSRVWSLG----AALAETLFDGGARTARNDAAMAAYDAAAAQYKQTV 311 GY SA DLFN SR + LG AL+ LFDGG R A D A A+Y+ A Y+ TV Sbjct: 346 GYESAQLGDLFNWSSRTFLLGPLVGTALSMPLFDGGRRQAGVDRARASYEEQVASYRGTV 405 Query: 312 LAGLQDVEDNLSTLRVLDQESAAQAQAVRSAQLAERLAMSQYQAGTVTYLSVVTTQATSL 371 L ++VEDNL+ LR+LDQ++ AQ +AV ++ A +L+ +QY+ G V+Y SV+ T L Sbjct: 406 LNAFREVEDNLANLRILDQQNRAQDEAVAASARAAKLSHTQYREGAVSYFSVIDADRTVL 465 Query: 372 TNQRSAVTLLGRQLVASVALIKATGGGWQV 401 QR++VTL G + ++V LI+A GGGW V Sbjct: 466 QQQRASVTLDGERARSTVNLIRALGGGWGV 495 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 205 bits (521), Expect = 6e-54, Method: Compositional matrix adjust. Identities = 132/316 (41%), Positives = 184/316 (58%), Gaps = 16/316 (5%) Query: 92 IQATLAQNYLQLRVTDLQKDLYRRTVEAYTRSLQLTTHQYEAGTALRSDVAQAETQLRSA 151 + A LA NY LR D++ D+ RR + ++L+L T +++ G D+AQ + L S Sbjct: 177 LTAELATNYFNLRELDVELDVVRRAIALQRQALELATARHDLGATSGLDMAQQQALLDST 236 Query: 152 QAQLIDLDATRNQLEHAIAMLIGKAPAAFSLAPLPAAAATDSGMDANALRLQASLPQIPA 211 Q+ L R Q EHAIA L G AFS+ P P IP Sbjct: 237 LTQIDLLGRQRAQYEHAIATLTGTPAPAFSITPSLTPITP---------------PAIPV 281 Query: 212 GLPSDLLERRPDIANAERLAAAANANIGVARAAYFPTLTLSTTGGYNSASFADLFNTPSR 271 G+PSD+LERRPDIA AER AAANA IGVA AAY+P+ L + G +S ++A LFN PS Sbjct: 282 GVPSDVLERRPDIAAAERAMAAANAQIGVASAAYYPSFMLQPSYGVDSRNWATLFNAPSL 341 Query: 272 VWSLGAALAETLFDGGARTARNDAAMAAYDAAAAQYKQTVLAGLQDVEDNLSTLRVLDQE 331 +WSLG + +++LFDGG A D + A Y+A A Y++TVL +Q+VED ++ L L++ Sbjct: 342 LWSLGVSASQSLFDGGRLRAGVDFSQAGYEATVASYRRTVLTAMQEVEDGITGLAALERA 401 Query: 332 SAAQAQAVRSAQLAERLAMSQYQAGTVTYLSVVTTQATSLTNQRSAVTLLGRQLVASVAL 391 A A+ SA+ +A S+Y+ G YL V+T Q + L ++R A L+G++L+ SV L Sbjct: 402 YAQSQLAIASARRVLEIASSRYEGGATPYLDVITAQQSLLNSERQAAQLMGQRLLVSVFL 461 Query: 392 IKATGGGWQVGQTDPA 407 IKA GG WQ +T PA Sbjct: 462 IKALGGDWQ-SRTPPA 476 Score = 74.3 bits (181), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 35/74 (47%), Positives = 44/74 (59%) Query: 12 GCAVGPDYVRPAMDLPQAYKEQGPWKVAAPRQADDHQRWWEAYGDPILNDLVNQANAANQ 71 GCA GPDY RP +D+P A+ + PW+ P A WWE +GD LN L QA A NQ Sbjct: 18 GCASGPDYTRPQVDMPAAWTSEAPWRSMQPADATPRGPWWERFGDAQLNALQQQALAGNQ 77 Query: 72 TIQQAAAQYRQAQA 85 T+ A A+ QA+A Sbjct: 78 TLAIATARLAQARA 91 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 150 bits (379), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 104/288 (36%), Positives = 156/288 (54%), Gaps = 17/288 (5%) Query: 116 TVEAYTRSLQLTTHQYEAGTALRSDVAQAETQLRSAQAQLIDLDATRNQLEHAIAMLIGK 175 T+E+ + S LT +E G++ + QA+T + SA+ + A +Q +A+ +L+GK Sbjct: 207 TLESQSSSYALTQRSFELGSSSALTLRQAQTSVESARVDVESYTAQVDQDRNALVLLVGK 266 Query: 176 -APAAFSLAPLPAAAATDSGMDANALRLQASLPQIPAGLPSDLLERRPDIANAERLAAAA 234 P A LP G+ L + L IP GLPSDLL+RR DI AER AA Sbjct: 267 DLPTAL----LP------QGLPDTRLAAASPLATIPPGLPSDLLQRRADIVQAERDLRAA 316 Query: 235 NANIGVARAAYFPTLTLSTTGGYNSASFADLFNTPSRVWSLGAALAETLFDGGARTARND 294 NANIG ARAA++P++TL+ G SA A LF S WS ++ +FDGGA A D Sbjct: 317 NANIGAARAAFYPSITLTVNAGTASARLAGLFKGGSGSWSFVPQISLPIFDGGANQANLD 376 Query: 295 AAMAAYDAAAAQYKQTVLAGLQDVEDNLSTLRVLDQESAAQAQAVRSAQLAERLAMSQYQ 354 A + D + AQY++ + ++V D L+ L ++ AQ V+++ A RL+ ++Y Sbjct: 377 IATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQEALVQASDEAFRLSQARYH 436 Query: 355 AGTVTYLSVVTTQATSLTNQRSAVTLLGRQL---VASVALIKATGGGW 399 +G +YL+V+ +Q T + Q++ L+G QL V K GGGW Sbjct: 437 SGVDSYLTVLDSQRTLYSAQKN---LIGTQLSRWTNLVTFYKTLGGGW 481 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.125 0.348 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 896,378 Number of extensions: 26575 Number of successful extensions: 203 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 176 Number of HSP's successfully gapped: 21 Length of query: 416 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 328 Effective length of database: 1,203,190 Effective search space: 394646320 Effective search space used: 394646320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)