BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0021.p (146 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311794|ref|YP_003775886.1| TetR family transcription regul... 239 6e-65 >gi|300311794|ref|YP_003775886.1| TetR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 239 Score = 239 bits (611), Expect = 6e-65, Method: Compositional matrix adjust. Identities = 117/131 (89%), Positives = 126/131 (96%) Query: 1 MEALLRAVEPLPPLGDSGSARDDIEQHMLQLSQLLRGKSGRVVREMIALAQFEPESRRIF 60 MEALLRAVEP+PP+GDSGS+R DIE HMLQLSQLLRGKSGRVVREMIALAQFEPES+RIF Sbjct: 103 MEALLRAVEPMPPIGDSGSSRTDIELHMLQLSQLLRGKSGRVVREMIALAQFEPESKRIF 162 Query: 61 NDNYLEPRRLALVNVLRRGAQRGEFREDADLNLVFDLLYAPVLQRLLTGSADIDERQIQS 120 NDNYLEPRRLALV+VLRRGAQRGEFREDADL+LVFDLLYAP+LQRLLTG DIDERQ+Q+ Sbjct: 163 NDNYLEPRRLALVSVLRRGAQRGEFREDADLSLVFDLLYAPLLQRLLTGQGDIDERQVQA 222 Query: 121 QLKLVLGSIAL 131 QLKLVLGSI L Sbjct: 223 QLKLVLGSITL 233 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 412,542 Number of extensions: 15626 Number of successful extensions: 60 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 60 Number of HSP's successfully gapped: 1 Length of query: 146 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 69 Effective length of database: 1,255,275 Effective search space: 86613975 Effective search space used: 86613975 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)