BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0014.p (261 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 454 e-129 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 140 9e-35 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 134 6e-33 gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 134 1e-32 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 133 1e-32 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 454 bits (1169), Expect = e-129, Method: Compositional matrix adjust. Identities = 218/261 (83%), Positives = 242/261 (92%) Query: 1 MPSLNPSQLQRFKDKVVIVTGAASGIGEATARRFSDEGAKVLLADRNAAALTKVFDSLPA 60 M +LNPS+LQRF+ KVVIVTGAASGIGEATARRFSDEGA+VLLADR+AAAL KVFDSLP Sbjct: 1 MSALNPSRLQRFEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPP 60 Query: 61 ERSAARETDVSHHEDVRQMVEFAIERFGRIDVLVSDAGIFAEGDITQVSPEDWHHVQATN 120 ER+AARETDVSHHE VRQ+V+FAIERFG++DVLVSDAG+FAEG++T+VSPEDWH VQATN Sbjct: 61 ERTAARETDVSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATN 120 Query: 121 VNGVFYGAREAMPHLEKTRGCIVNVASVSGLGADWNLAAYNTSKGAVVNLTRAMALDFGR 180 VNGVFYGAREA+PHLEKTRGCIVNVASVSGL ADWNL+AYN SKGAV NLTRAMALDFGR Sbjct: 121 VNGVFYGAREALPHLEKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGR 180 Query: 181 KGIRVNAVCPSLTYTGMTADVAEDQEQLAKFAERIPLGRGADPVEIASVIAFLASPDAGF 240 KG+R+NAVCPSLT+T MTAD+A+D L KFAERI LGRGADP+EIA+VI FLASPDA F Sbjct: 181 KGVRINAVCPSLTHTAMTADMADDPPLLDKFAERIALGRGADPLEIAAVITFLASPDASF 240 Query: 241 VTGVNLPVDGGVTASNGQPPQ 261 V GVNLPVDGG+ ASNGQPPQ Sbjct: 241 VNGVNLPVDGGLMASNGQPPQ 261 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 140 bits (354), Expect = 9e-35, Method: Compositional matrix adjust. Identities = 90/249 (36%), Positives = 144/249 (57%), Gaps = 7/249 (2%) Query: 10 QRFKDKVVIVTGAASGIGEATARRFSDEGAKVLLADRNAAALTKVFDSLPAE--RSAARE 67 R +DKV IVTG SG GE A+ ++ EGA +++AD A +V + + A R+ + Sbjct: 1 MRLQDKVAIVTGGGSGFGEGIAKAYAREGAAIVVADIGEAGGLRVVEEIKAAGGRAVFAK 60 Query: 68 TDVSHHEDVRQMVEFAIERFGRIDVLVSDAGIFAEGD-ITQVSPEDWHHVQATNVNGVFY 126 DVS D+ Q++ A+E FG++D++V++AG + +V +++ V A NV +F Sbjct: 61 ADVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEDEFDRVYAVNVKSIFL 120 Query: 127 GAREAMPHLEKTRG-CIVNVASVSGLGADWNLAAYNTSKGAVVNLTRAMALDFGRKGIRV 185 A+ +P+ + G +N+AS +G+ L YN SKGAV+ +++MA + G IRV Sbjct: 121 SAKTFVPYFRQVGGGAFINIASTAGIRPRPGLTWYNGSKGAVITTSKSMAAELGPDKIRV 180 Query: 186 NAVCPSLTYTGMTAD---VAEDQEQLAKFAERIPLGRGADPVEIASVIAFLASPDAGFVT 242 N V P ++ TG+ ++ V + E KF IP+GR + P +IA+ +L S +A FVT Sbjct: 181 NCVNPVISATGLLSEFMGVPDTPENRKKFVATIPMGRFSTPEDIANACLYLGSDEAEFVT 240 Query: 243 GVNLPVDGG 251 GV + VDGG Sbjct: 241 GVCIEVDGG 249 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 134 bits (338), Expect = 6e-33, Method: Compositional matrix adjust. Identities = 85/243 (34%), Positives = 132/243 (54%), Gaps = 4/243 (1%) Query: 15 KVVIVTGAASGIGEATARRFSDEGAKVLLADRNAAALTKVFDSLPA--ERSAARETDVSH 72 +V +VTG A GIG + +++ + +G VL+AD N A +K D+L A R+A + D+ Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAAPLQMDLGS 68 Query: 73 HEDVRQMVEFAIERFGRIDVLVSDAGIFAEGDITQVSPEDWHHVQATNVNGVFYGAREAM 132 E + + +GR DVLV++AG+ E+W NV GV + A Sbjct: 69 PESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAGQHAA 128 Query: 133 PHLEKTR-GCIVNVASVSGLGADWNLAAYNTSKGAVVNLTRAMALDFGRKGIRVNAVCPS 191 + K + G IVN++S+SG+ A AY TSK AV+ LTR MA++ + GI VN+V P Sbjct: 129 RLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSVAPG 188 Query: 192 LTYTGMTADVAEDQEQLAKFAERIPLGRGADPVEIASVIAFLASPDAGFVTGVNLPVDGG 251 T MT + +Q + + +P+ R EIAS + +LA+ ++ ++TG +PVDGG Sbjct: 189 PVDTPMTQAMHSEQTR-ESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGHVIPVDGG 247 Query: 252 VTA 254 A Sbjct: 248 FVA 250 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 134 bits (336), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 88/255 (34%), Positives = 132/255 (51%), Gaps = 5/255 (1%) Query: 6 PSQLQRF--KDKVVIVTGAASGIGEATARRFSDEGAKVLLADRNAAALTKVFDSLPAERS 63 P+ LQ F + K+ ++TG++ GIG A AR + GA ++L R+A L V L AE Sbjct: 9 PAGLQGFSLEGKLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVAAQLRAEGH 68 Query: 64 AARET--DVSHHEDVRQMVEFAIERFGRIDVLVSDAGIFAEGDITQVSPEDWHHVQATNV 121 E+ D + E+V+ V+ G I +LV++AGI + + DWH + +TN+ Sbjct: 69 TIHESSFDATSSEEVKAAVQKIESEIGPIGILVNNAGIQRRNPLEEFKESDWHDLISTNL 128 Query: 122 NGVFYGAREAMPHL-EKTRGCIVNVASVSGLGADWNLAAYNTSKGAVVNLTRAMALDFGR 180 N VF+ A+ H+ + G I+N+ SV +A Y +KGAV T+ MA+D+G+ Sbjct: 129 NSVFFAAQAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAIDWGK 188 Query: 181 KGIRVNAVCPSLTYTGMTADVAEDQEQLAKFAERIPLGRGADPVEIASVIAFLASPDAGF 240 GI+VN + P T M + D + R P R D ++ FLAS + F Sbjct: 189 YGIQVNGLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASDASRF 248 Query: 241 VTGVNLPVDGGVTAS 255 V G L VDGGVTA+ Sbjct: 249 VNGHILYVDGGVTAT 263 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 133 bits (335), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 87/247 (35%), Positives = 134/247 (54%), Gaps = 6/247 (2%) Query: 10 QRFKDKVVIVTGAASGIGEATARRFSDEGAKVL---LADRNAAALTKVFDSLPAERSAAR 66 Q + +V +VTGA+ GIG A A + +GAKV+ ++ AAA+T+ +L E Sbjct: 4 QNLQGQVALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGPEAGRGA 63 Query: 67 ETDVSHHEDVRQMVEFAIERFGRIDVLVSDAGIFAEGDITQVSPEDWHHVQATNVNGVFY 126 +V+ E +VE + FG + +LV++AGI + ++ E+W V ATN+ V Sbjct: 64 VLNVNDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGR 123 Query: 127 GAREAMPHLEKTR-GCIVNVASVSGLGADWNLAAYNTSKGAVVNLTRAMALDFGRKGIRV 185 +R + + K R G I+N+ SV G + Y +K V ++RA+A + G + I V Sbjct: 124 LSRAVLRGMMKARTGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNITV 183 Query: 186 NAVCPSLTYTGMTADVAEDQEQLAKFAERIPLGRGADPVEIASVIAFLASPDAGFVTGVN 245 N V P T MT A +++Q A ++IPLGR EIA +AFLASP A ++TG Sbjct: 184 NCVAPGFIDTDMTR--ALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGTT 241 Query: 246 LPVDGGV 252 L V+GG+ Sbjct: 242 LNVNGGM 248 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 808,023 Number of extensions: 33574 Number of successful extensions: 285 Number of sequences better than 1.0e-08: 50 Number of HSP's gapped: 177 Number of HSP's successfully gapped: 50 Length of query: 261 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 178 Effective length of database: 1,226,865 Effective search space: 218381970 Effective search space used: 218381970 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)