BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0013.p (810 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311789|ref|YP_003775881.1| chemotaxis histidine kinase [He... 1446 0.0 gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [He... 109 1e-24 gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbas... 94 5e-20 gi|300312286|ref|YP_003776378.1| signal-transducing histidine ki... 93 7e-20 gi|300313133|ref|YP_003777225.1| CheA-like histidine kinase [Her... 90 6e-19 >gi|300311789|ref|YP_003775881.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 808 Score = 1446 bits (3742), Expect = 0.0, Method: Compositional matrix adjust. Identities = 743/810 (91%), Positives = 773/810 (95%), Gaps = 2/810 (0%) Query: 1 MTIRMRITLLVILTFVAIAGIGGYAMMQSRSSAVEVRSVTEGVMPSVLASADLMGQLKDV 60 MTIRMRITLLVILTF+AIAGIGGYAM QSRSSAVEVRSVTEGVMPSVLASADLMGQLKDV Sbjct: 1 MTIRMRITLLVILTFIAIAGIGGYAMYQSRSSAVEVRSVTEGVMPSVLASADLMGQLKDV 60 Query: 61 QLAAMVMVNETDNQLVAQENDKLAEKKAVLEKALALQAQQAEGVAQRGLIEQAKESLDNY 120 QLAAMVMV E DNQLVAQEN+KLAEKKAVLEKALA QAQQAEGVAQRGLIEQAKESLDNY Sbjct: 61 QLAAMVMVTENDNQLVAQENEKLAEKKAVLEKALAAQAQQAEGVAQRGLIEQAKESLDNY 120 Query: 121 FGAIDTTAKFKLAGKNEIAQAQFFGGVMQYQTELEGIVNTLRIEKNRFKDNAIASLNGKL 180 FGAIDTTAKFKLAGKNEIAQAQFFGGVMQYQTELEGIVNTLRIEKNRFKDNAIASLNGKL Sbjct: 121 FGAIDTTAKFKLAGKNEIAQAQFFGGVMQYQTELEGIVNTLRIEKNRFKDNAIASLNGKL 180 Query: 181 AQTTTTISIVTVVAVLLLGGLGFLLYRQITGPISRMAAMMGEIATSQDFTRRLPVQRMDE 240 AQTTTTISIVTV+AVLLLGG+GFLLYRQITGPISRMAAMMGEIATSQDFTRRLPVQRMDE Sbjct: 181 AQTTTTISIVTVMAVLLLGGIGFLLYRQITGPISRMAAMMGEIATSQDFTRRLPVQRMDE 240 Query: 241 VGHSIVAFNGMVEKIEEASAQVRQKTADMQAMLQNIPQGILTIVDGNKVHPEYSAFLETV 300 VGHSIVAFNGMVEKIEEASAQVRQKTADMQAMLQNIPQGILTIV+GN++HPEYSAFLETV Sbjct: 241 VGHSIVAFNGMVEKIEEASAQVRQKTADMQAMLQNIPQGILTIVEGNRIHPEYSAFLETV 300 Query: 301 FDTTDIAGRDVMELVFSNTNLGADTLSQIEAIGGACIGEDVMNFSFNEHLMVGEIEKTMA 360 F+TTDIAGRDVMELVF++T LGADTLSQIEAIGGACIGEDVMNF FN HLMVGEIEKTMA Sbjct: 301 FETTDIAGRDVMELVFADTQLGADTLSQIEAIGGACIGEDVMNFEFNAHLMVGEIEKTMA 360 Query: 361 DGSVKILDLSWSPITDEQDMVIRLMLCVRDVTELRHLAAEANEQKRELEMIGEILAVAPA 420 DGS KILDLSWSPITDE D VIRLMLCVRDVTELR LAAEANEQKRELEMIGEILAV PA Sbjct: 361 DGSKKILDLSWSPITDEADTVIRLMLCVRDVTELRQLAAEANEQKRELEMIGEILAVTPA 420 Query: 421 KFAEFIVSARRFLDENEQIVHQNPQATAFAIAKLFRNMHTIKGNGRTYGLLHLANVVHHT 480 KFAEFI SARRFLDENEQIVHQNP+ATAFAIAKLFRNMHTIKGNGRTYGLLHLANVVHHT Sbjct: 421 KFAEFISSARRFLDENEQIVHQNPEATAFAIAKLFRNMHTIKGNGRTYGLLHLANVVHHT 480 Query: 481 EQHYDDLRHPASGAVWNQEELLLQLAQVREIIDRYARINDVTLGRGTSAGGGGEQVLPGY 540 EQHYDDLRHPASGAVW QEELL QLAQVREIIDRYARINDVTLGRG +A GEQ LPGY Sbjct: 481 EQHYDDLRHPASGAVWRQEELLRQLAQVREIIDRYARINDVTLGRGVAA--AGEQTLPGY 538 Query: 541 ERHVLTIDRKHIQESLLRLENVNTANLHELVAVRNDVRRTLRLLGTETIEEVLAPVIDSL 600 RHV+TIDRKHI++SL RLENVNTANLHELVAVRNDVRRTLRLLGTETI+EVLAPV DSL Sbjct: 539 ARHVITIDRKHIEQSLERLENVNTANLHELVAVRNDVRRTLRLLGTETIQEVLAPVTDSL 598 Query: 601 PALASDLGKSAPIVQIDDNGYVVHSFASPLLQNVFMHLIRNAVDHGLESGEERLAKGKPE 660 PALA+DLGK+AP+VQI+DNGYVVHSFA+PLLQNVFMHLIRNA+DHGLES EERLAKGKPE Sbjct: 599 PALAADLGKAAPVVQIEDNGYVVHSFAAPLLQNVFMHLIRNAMDHGLESSEERLAKGKPE 658 Query: 661 AGTIKLEMGVMEDMFQMALSDDGRGLALHKVRQIAIEKQLISVDQVLTDAEIARLILQPG 720 AG IKLEMGVMEDMFQMALSDDGRGLAL +VRQI IEK LI+VDQ L+DAEIARLILQPG Sbjct: 659 AGVIKLEMGVMEDMFQMALSDDGRGLALERVRQIGIEKHLITVDQALSDAEIARLILQPG 718 Query: 721 FSTRSEVTEVSGRGVGMDAVLDFVTREHGKIEIRFMDSEEGAAFRAFQIIVMLPENVAVE 780 FSTRSEVTEVSGRGVGMDAVLDFVTREHG+IEIRF D+EEGA FR+FQIIVMLPENVAVE Sbjct: 719 FSTRSEVTEVSGRGVGMDAVLDFVTREHGQIEIRFTDTEEGAPFRSFQIIVMLPENVAVE 778 Query: 781 VDGIDVPYPVAGQEIAAPAVLPPGGDAQVA 810 VDGIDVPYP+ GQE P VLP GGD+QVA Sbjct: 779 VDGIDVPYPMGGQETVQPVVLPQGGDSQVA 808 >gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 725 Score = 109 bits (272), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 76/215 (35%), Positives = 109/215 (50%), Gaps = 32/215 (14%) Query: 603 LASDLGKSAPIVQIDDN----GYVVHSFASPLLQNVFMHLIRNAVDHGLESGEERLAKGK 658 +A DLGK +V + +V A PL MH++RNA+DHG+E ERLA GK Sbjct: 410 IARDLGKDIDLVMTGSDTELDKSMVERLADPL-----MHIVRNAIDHGIEPAAERLAAGK 464 Query: 659 PEAGTIKLEMGVMEDMFQMALSDDGRGLALHKVRQIAIEKQLISVDQVLTDAEIARLILQ 718 P +++L + + DDGRG+ + A+ + L S D L+DAEI R + + Sbjct: 465 PAKASLQLNAIHESGSVVIEVMDDGRGMDREAILAKAVAQGLASPDAELSDAEIYRFVFE 524 Query: 719 PGFSTRSEVTEVSGRGVGMDAVLDFVTREHGKIEIRFMDSEEG-------------AAFR 765 PGFST +VTE+SGRGVGMD V + HG++ I D+ G A Sbjct: 525 PGFSTARQVTELSGRGVGMDVVRRNIEALHGEVSI---DTGRGRGTVVRLRLPLTLAIIS 581 Query: 766 AFQIIV-----MLPENVAVEVDGIDVPYPVAGQEI 795 FQ++V ++P ++ VE ID+P G I Sbjct: 582 GFQVVVGNAVFVIPLDMVVEC--IDMPPQQPGSHI 614 >gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbaspirillum seropedicae SmR1] Length = 638 Score = 93.6 bits (231), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 60/155 (38%), Positives = 84/155 (54%), Gaps = 11/155 (7%) Query: 603 LASDLGKSAPIV----QIDDNGYVVHSFASPLLQNVFMHLIRNAVDHGLESGEERLAKGK 658 LA DLGK +V + + + VV S PL+ H++RN++DHG+E +R+A GK Sbjct: 338 LAKDLGKDVDLVITGAETEVDRVVVDSLYDPLV-----HMLRNSLDHGIEV--DRVAAGK 390 Query: 659 PEAGTIKLEMGVMEDMFQMALSDDGRGLALHKVRQIAIEKQLISVDQVLTDAEIARLILQ 718 P TI L + + DDG+G+ +R A+ K LI+ Q LT E +L+ Sbjct: 391 PTKATISLNAWQAGSSVMIEVFDDGKGMDPDMLRSKALAKGLINEHQALTADEALQLVFL 450 Query: 719 PGFSTRSEVTEVSGRGVGMDAVLDFVTREHGKIEI 753 PGFST+ + VSGRGVGMD V V + G I I Sbjct: 451 PGFSTKEVASSVSGRGVGMDVVKTAVEKHRGTIRI 485 >gi|300312286|ref|YP_003776378.1| signal-transducing histidine kinase CheA protein [Herbaspirillum seropedicae SmR1] Length = 697 Score = 93.2 bits (230), Expect = 7e-20, Method: Compositional matrix adjust. Identities = 70/191 (36%), Positives = 105/191 (54%), Gaps = 23/191 (12%) Query: 603 LASDLGKSAPIVQIDDNGYVVHSFASPL-------LQNVFMHLIRNAVDHGLESGEERLA 655 LA LGK Q++ +V H A+ L + + HL+RN++DHG+E + R A Sbjct: 386 LAHKLGK-----QVE---FVTHGAATELDKGLIERIVDPLTHLVRNSIDHGIELPDARRA 437 Query: 656 KGKPEAGTIKLEMGVMEDMFQMALSDDGRGLALHKVRQIAIEKQLISVDQVLTDAEIARL 715 GK EAGT+ L + ++DDG GL K+ A ++Q + V L+DAE+ +L Sbjct: 438 SGKSEAGTLSLSAEHQGGNIIIEVTDDGGGLNREKILAKA-KQQGMPVTDNLSDAEVWQL 496 Query: 716 ILQPGFSTRSEVTEVSGRGVGMDAVLDFVTREHGKIEIRFMDSEEGAAFRAFQIIVMLPE 775 I +PGFST +VT+VSGRGVGMD V + G ++IR S +G I + LP Sbjct: 497 IFEPGFSTAEQVTDVSGRGVGMDVVKRNILAMGGAVDIR---SSKGF---GTTISISLPL 550 Query: 776 NVAVEVDGIDV 786 +A+ +DG+ + Sbjct: 551 TLAI-LDGMSI 560 >gi|300313133|ref|YP_003777225.1| CheA-like histidine kinase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 90.1 bits (222), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 56/150 (37%), Positives = 85/150 (56%), Gaps = 7/150 (4%) Query: 631 LQNVFMHLIRNAVDHGLESGEERLAKGKPEAGTIKLEMGVMEDMFQMALSDDGRGLALHK 690 L HL+RNA HG+ES E+RLA+GKP G + ++ + + +SDDGRGL L Sbjct: 457 LMGPLEHLLRNAAVHGIESAEQRLAEGKPAVGRLTVQATHEGNEAVIRVSDDGRGLDLEA 516 Query: 691 VRQIAIEKQLISVDQVLTDAEIARLILQPGFSTRSEVTEVSGRGVGMDAVLDFVTREHGK 750 +RQ A+E + + + A +A LI QPG ST +V+ ++GRG+GMD V V G Sbjct: 517 IRQKALELGAAGLAEA-SQARLADLIFQPGLSTSPQVSALAGRGIGMDVVRSEVAALGGW 575 Query: 751 IEIRFMDSEEGAAFRAFQIIVMLPENVAVE 780 + + S+ G + Q + LP ++AV+ Sbjct: 576 MRVH---SQPG---QGVQFTLYLPLSLAVQ 599 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,186,525 Number of extensions: 86374 Number of successful extensions: 402 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 407 Number of HSP's successfully gapped: 10 Length of query: 810 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 717 Effective length of database: 1,179,515 Effective search space: 845712255 Effective search space used: 845712255 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 135 (56.6 bits)