BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0019.0005.p
         (438 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311782|ref|YP_003775874.1| chloride channel protein [Herba...   707   0.0  
gi|300311435|ref|YP_003775527.1| chloride channel protein [Herba...    62   9e-11

>gi|300311782|ref|YP_003775874.1| chloride channel protein
           [Herbaspirillum seropedicae SmR1]
          Length = 442

 Score =  707 bits (1824), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 377/440 (85%), Positives = 400/440 (90%), Gaps = 7/440 (1%)

Query: 1   MKNQKLPEQLHLLPYIVKWCLIASLIAALAGSASAFFLFALDYATHWRETHPWIIWLLPL 60
           MKN   PEQL+LLPYI KW LIA LIAALAGSASAFFLFALD+ATHWRE H WIIWLLPL
Sbjct: 1   MKNHHYPEQLYLLPYIGKWTLIACLIAALAGSASAFFLFALDWATHWRERHAWIIWLLPL 60

Query: 61  GGFAVGWLYLKTGKPVEAGNNLLIDEIHDPKKVIPLRMAPLVLFGTVMSHLFGASVGREG 120
           GGFAVGWLYL TGKPVEAGNNLLIDEIHDP+KVIPLRMAPLVL GTV SHLFGASVGREG
Sbjct: 61  GGFAVGWLYLHTGKPVEAGNNLLIDEIHDPRKVIPLRMAPLVLLGTVASHLFGASVGREG 120

Query: 121 TAVQMGGALADQLTTVLRLRTEDRRILLMAGISAGFASVFGTPMAGALFGLEVLAIGRMR 180
           TAVQMGGALADQLT +LRLR EDRRILLMAGISAGF+SVFGTPMAGA+FGLEVLAIGRMR
Sbjct: 121 TAVQMGGALADQLTHLLRLRPEDRRILLMAGISAGFSSVFGTPMAGAIFGLEVLAIGRMR 180

Query: 181 YDAILPCFVAAIAADQVGLAWGVHHTHYHMGGVVPVALWSVAAVIAAGVVFGLVGMVFAN 240
           Y+AI PCFVAAIAADQVGLAWGVHHTHY M    P+ALWSVAAVIAAGVVFGL GM+FAN
Sbjct: 181 YEAIFPCFVAAIAADQVGLAWGVHHTHYAMNASAPMALWSVAAVIAAGVVFGLAGMIFAN 240

Query: 241 STHRLSAFMKRHVSYAPLRPFIGGAIVAVAVWALGTQRYIGLGIPVIVEAFQQPLAPWDF 300
           STH LSAFMKRHVSYAPLRPFIGG IVA+AVWA+GT RYIGLGIPVIVEAFQQPLAPWDF
Sbjct: 241 STHTLSAFMKRHVSYAPLRPFIGGFIVALAVWAVGTHRYIGLGIPVIVEAFQQPLAPWDF 300

Query: 301 LGKMVFTVTSLGTGFKGGEVTPLFYIGATVGNALAPLLNLPFSMLAGIGFVAVFAGAANT 360
           LGKMVFTVTSLG+GFKGGEVTPLFYIGAT+GNALAPLL+LPF +LAGIGFVAVFAGAANT
Sbjct: 301 LGKMVFTVTSLGSGFKGGEVTPLFYIGATLGNALAPLLHLPFPLLAGIGFVAVFAGAANT 360

Query: 361 PIASTIMAVELFGPEIGPFAALACVVAYLFSGHTGIYHAQRVGQGKYLSLPEGLKIGEIG 420
           P+ASTIMA+ELFGP+IGPFAALACVVAYLFSGHTGIYHAQRVGQ K   LP+GL+IGE+G
Sbjct: 361 PLASTIMAIELFGPQIGPFAALACVVAYLFSGHTGIYHAQRVGQAKLGLLPKGLRIGEVG 420

Query: 421 AYR-------ARRQAERDQE 433
            YR       A + +E DQ 
Sbjct: 421 GYRRKLSAAPAEQASELDQH 440


>gi|300311435|ref|YP_003775527.1| chloride channel protein
           [Herbaspirillum seropedicae SmR1]
          Length = 555

 Score = 62.0 bits (149), Expect = 9e-11,   Method: Compositional matrix adjust.
 Identities = 99/401 (24%), Positives = 162/401 (40%), Gaps = 50/401 (12%)

Query: 52  PWIIW-LLPLGGFAVGWLYLKTGKPVEAGNN-LLIDEIHDPKKVIPLRMAPLVLFGTVMS 109
           PW    LLP  G  V  L+L   K   AGN    ++ +      IP+R   L    ++ S
Sbjct: 45  PWYTRVLLPTCGGVVAGLFLVWAKRTPAGNGGDYMEAVAIGDGAIPVRNTLLRSISSLAS 104

Query: 110 HLFGASVGREGTAVQMGGALADQLTTVLRLRTEDRRILLMAGISAGFASVFGTPMAGALF 169
              G S+GREG  VQ+    A  +       +   R+L+  G +AG  S +  P+AGA F
Sbjct: 105 IASGGSIGREGPMVQLSALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFF 164

Query: 170 GLEVLAIGRMRYDAILPCFVAAIAADQVGLAWGVHHTHYHMGGVVPVALWSVAAVIAAGV 229
             E++ +G +  ++  P  VA++ A+        +   Y M     +  W V   +  GV
Sbjct: 165 ITEIV-LGSLVMESFGPVVVASVVANITMRELPGYRAAYEMPYFPEIGGWEVMLFLVLGV 223

Query: 230 VFGLVGMVFANSTHRLSAFMKRHVSYAPLRPFIGG------AIVAVAVWALGTQRYIGLG 283
           + G++   F            +     P+R  +GG      ++    VW  G        
Sbjct: 224 LAGVLAPQFLRVLELGKHGFGKLRLPLPVRLGVGGLLVGLISVQVPEVWGNGYS------ 277

Query: 284 IPVIVEAFQQPLAPWD-----FLGKMVFTVTSLGTGFKGGEVTPLFYIGATVG------- 331
              +V +       W       + K++ T  ++G+G  GG  TP  ++GA VG       
Sbjct: 278 ---LVNSLLHTSWVWQAVLMVLVVKVLATSITVGSGAVGGIFTPTLFVGAAVGYLFGDAA 334

Query: 332 NALAPL-LNLPFSMLAGIGFVAVFAGAANTPIASTIMAVEL-FGPEIGPFAALACVVAYL 389
            AL P+ ++ PF+  A +G  A  A A+  P+ + +M  E+    ++     L+CVVAY+
Sbjct: 335 QALLPVHISQPFAY-AMVGMGAFLAAASYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYV 393

Query: 390 FSGHTGIYHAQRVGQGKYLSLPEGLKIGEIGAYRARRQAER 430
            +                    +G  + EI   R R   ER
Sbjct: 394 VARSV-----------------DGRSMYEITLKRHRDNEER 417


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.326    0.141    0.439 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,312,935
Number of extensions: 53786
Number of successful extensions: 258
Number of sequences better than 1.0e-08: 4
Number of HSP's gapped: 251
Number of HSP's successfully gapped: 4
Length of query: 438
Length of database: 1,619,870
Length adjustment: 88
Effective length of query: 350
Effective length of database: 1,203,190
Effective search space: 421116500
Effective search space used: 421116500
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 132 (55.5 bits)