BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0009.p (471 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309611|ref|YP_003773703.1| outer membrane channel lipoprot... 638 0.0 gi|300311703|ref|YP_003775795.1| ABC-type export system outer me... 187 1e-48 gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 135 5e-33 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 129 3e-31 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 125 9e-30 >gi|300309611|ref|YP_003773703.1| outer membrane channel lipoprotein [Herbaspirillum seropedicae SmR1] Length = 493 Score = 638 bits (1646), Expect = 0.0, Method: Compositional matrix adjust. Identities = 353/460 (76%), Positives = 395/460 (85%), Gaps = 15/460 (3%) Query: 19 SGCALGPTGETPAMPQPVHYAAEATPATLGPQEARQTFVLGQRSVPQWWRAFGSADLSAW 78 SGCA GPTGE PAMP+P HYAA+ATPA +GP+ ARQ FVLGQR+VPQWW+A+GS +LSAW Sbjct: 19 SGCAFGPTGEAPAMPRPDHYAAQATPADVGPEGARQRFVLGQRAVPQWWQAYGSPELSAW 78 Query: 79 VEEALRSNHSLAEADRNLASARLQLRAQIDESLWPTIDAVGQAQRQRALGLPNLGPTTNL 138 VEEALR+NHSLA+A+RNLASARLQLRAQID+SLWP++DAVGQAQRQRALGLPN+GPTTNL Sbjct: 79 VEEALRNNHSLAQAERNLASARLQLRAQIDQSLWPSVDAVGQAQRQRALGLPNIGPTTNL 138 Query: 139 YNVFAGQIRASYTFDLFGVERFANSALAAQVDAQSPPPPALLGALP-------------- 184 YNVFAGQIRASYTFDLFG ER+AN+A AAQVDAQS A AL Sbjct: 139 YNVFAGQIRASYTFDLFGTERYANAATAAQVDAQSYQFDAARRALVANVVISAINAAALR 198 Query: 185 -QVADTERLVALADADVGELQRRLALGAVSNDDVLSARANAQSLRASLPALKAQWQGARH 243 QV+DTERLVALA+AD E+QRR LGA S DD+L+ARANAQSLRASLP L+AQW ARH Sbjct: 199 VQVSDTERLVALAEADASEVQRRAELGAASQDDLLAARANAQSLRASLPGLQAQWLAARH 258 Query: 244 ALAVLMGRTPDAAPADLDITRLKLPEQVPMTVPSELLKQRPDILAADAALKVAAAQQALA 303 ALAVLMGRTPD APADLD+ L+LP +VP+ VPSELL+QRPDILAADAALKVAAAQQA A Sbjct: 259 ALAVLMGRTPDQAPADLDLASLRLPAEVPLAVPSELLRQRPDILAADAALKVAAAQQAQA 318 Query: 304 TAEMFPSLSISASFGQSGFSWAKATSGAGAVWGLGASLTQPLFHGGALRAKRDASKEAYL 363 TAEMFPSLSISASFGQSGF+W KAT AGAVWGLGASLTQPLFHGGALR+ +A+++AYL Sbjct: 319 TAEMFPSLSISASFGQSGFTWPKATGAAGAVWGLGASLTQPLFHGGALRSMSEAAEQAYL 378 Query: 364 AAEAHYRQTVLNAFQNVADSLVALTQDADALQASAAARAATEQGWRNAQRRAQLGAYPAS 423 AAEA+YRQTVLNAFQNVADSLVAL+ DADA A+AAAR A+EQGWRNAQRR LGA P S Sbjct: 379 AAEANYRQTVLNAFQNVADSLVALSHDADAQTAAAAARQASEQGWRNAQRRVALGAVPIS 438 Query: 424 AARAGERQYLNARLGEIRATASRMNDTATLYQAMGVGLDA 463 AAR+GERQYLNA L E RATASR+N TATL+QAMGV L+A Sbjct: 439 AARSGERQYLNAHLSEARATASRLNATATLFQAMGVSLEA 478 >gi|300311703|ref|YP_003775795.1| ABC-type export system outer membrane channel protein [Herbaspirillum seropedicae SmR1] Length = 489 Score = 187 bits (476), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 154/465 (33%), Positives = 236/465 (50%), Gaps = 29/465 (6%) Query: 19 SGCALGPTGETPAMPQPVHYAAEATPATL---GPQEARQTFVLGQRSVPQWWRAFGSADL 75 +GC +GP P P+ Y + P L + Q ++G+ +WW+ FGS +L Sbjct: 22 AGCLVGPDFVAPTPPRVSQYTRD--PVLLDRMSEPDMSQQLLMGENPPARWWQLFGSPEL 79 Query: 76 SAWVEEALRSNHSLAEADRNLASARLQLRAQIDESLWPTIDAVGQAQRQRALGLPNLGPT 135 A V +AL N +L A NL A LRA +P +DA A+R R+ L G + Sbjct: 80 DAAVTQALTRNPTLQAAVSNLLLAEDNLRAG-GAVFYPRLDANMMAERTRSASLVQ-GSS 137 Query: 136 T--NLYNVFAGQIRASYTFDLFGVERFANSALAAQVDAQSPPPPALL------------- 180 +++V SY DLFG +R L A VD Q A Sbjct: 138 APDKVFSVATLSGAVSYAIDLFGEKRRTLEQLEAMVDVQQQLHHAAYLTLTANVVNAYIA 197 Query: 181 --GALPQVADTERLVALADADVGELQRRLALGAVSNDDVLSARANAQSLRASLPALKAQW 238 G L Q++ +++AL + + +Q R+ G D+LS + +A L L + Sbjct: 198 RAGYLSQISLVRQIIALEELQLEAIQARVDTGVAPLADLLSQASLLAGNQALLAQLTQRL 257 Query: 239 QGARHALAVLMGRTP-DAAPADLDITRLKLPEQVPMTVPSELLKQRPDILAADAALKVAA 297 H LA L G P A+ +L + L LP +P+++PS L+++RPDILAA+A + A+ Sbjct: 258 YQIEHLLAQLQGEFPAQASLPELVLADLHLPSDIPVSLPSALVRRRPDILAAEARMHAAS 317 Query: 298 AQQALATAEMFPSLSISASFGQSGFSWAKATSGAGAVWGLGASLTQPLFHGGALRAKRDA 357 A +++A +PSL ++A++G + + ++ T A ++W G SL+ PLF+GG LRA+RDA Sbjct: 318 AAIGMSSAAQYPSLELTATYGVAARNLSQLTGPATSLWSFGPSLSTPLFNGGRLRAQRDA 377 Query: 358 SKEAYLAAEAHYRQTVLNAFQNVADSLVALTQDADALQA-SAAARAATEQ-GWRNAQRRA 415 + A+ A+++ Y+QTVL AF+ VAD+L AL D + A SAAAR A + + A A Sbjct: 378 AVNAFEASQSEYQQTVLTAFEQVADALRALQADGHIVSAQSAAARYAYDTLLLQRASYSA 437 Query: 416 QLGAYPASAARAGERQYLNARLGEIRATASRMNDTATLYQAMGVG 460 L +Y + E QY +A + ++AT R DT L A G G Sbjct: 438 GLSSY--ADMLLYEVQYRSAAIASLQATVQRYQDTVALLVASGGG 480 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 135 bits (341), Expect = 5e-33, Method: Compositional matrix adjust. Identities = 152/486 (31%), Positives = 225/486 (46%), Gaps = 54/486 (11%) Query: 20 GCALGPTGETPAMPQPVHYAAEATPATLGPQEARQTFVLGQRSVP-------QWWRAFGS 72 GC++ PT E P++ P + EA PAT + Q + + P +WW+ FG Sbjct: 30 GCSVAPTYERPSVDTPAAFK-EAAPATQTGENGSQW----KTAQPAEDIARGEWWKIFGD 84 Query: 73 ADLSAWVEEALRSNHSLAEADRNLASARLQLRAQIDESLWPTIDAVGQAQRQR----ALG 128 L+A + A ++N L A L AR R +P +DA A RQR + G Sbjct: 85 EKLNALEDTAQQANQDLKAAAARLGQARALTR-DARAGYFPQVDAGVGATRQRPSPASQG 143 Query: 129 LP---NLGPTTNLYNVFAGQIRASYTFDLFGVERFANSALAAQVDAQSPP---PPALLGA 182 LP + P T ++ Q+ SY DLFG R A++ AA DAQ LL Sbjct: 144 LPADADTRPNT----LYRAQVNVSYEVDLFG--RVASTVDAATADAQRSEALFHSVLLAL 197 Query: 183 LPQVADTERLVALADA---------DVGE-----LQRRLALGAVSNDDVLSARANAQSLR 228 VA LV DA D+ + +QRR G +S DV ARA S + Sbjct: 198 QADVAQQYFLVRELDAAAALYRGTVDLRQESLRLVQRRFDEGDISEVDVARARAELASAQ 257 Query: 229 ASLPALKAQWQGARHALAVLMGRTP-DAAPADLDITRLKLPEQVPMTVPSELLKQRPDIL 287 + + Q A HALA+L+GRTP + + +TRL + VP +PS LL++RPDI Sbjct: 258 SEALGIARQRATAEHALAILLGRTPAEFSLPPQPLTRLAV--TVPPGLPSALLERRPDIA 315 Query: 288 AADAALKVAAAQQALATAEMFPSLSISASFGQSG------FSWAKATSGAGAVWGLGASL 341 AA+ A+ A A+ +A + FPSL+++ G F+W+ T G + +G +L Sbjct: 316 AAERAMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSRTFLLGPL--VGTAL 373 Query: 342 TQPLFHGGALRAKRDASKEAYLAAEAHYRQTVLNAFQNVADSLVALTQDADALQASAAAR 401 + PLF GG +A D ++ +Y A YR TVLNAF+ V D+L L +A A Sbjct: 374 SMPLFDGGRRQAGVDRARASYEEQVASYRGTVLNAFREVEDNLANLRILDQQNRAQDEAV 433 Query: 402 AATEQGWRNAQRRAQLGAYPASAARAGERQYLNARLGEIRATASRMNDTATLYQAMGVGL 461 AA+ + + + + + GA + +R L + + R T L +A+G G Sbjct: 434 AASARAAKLSHTQYREGAVSYFSVIDADRTVLQQQRASVTLDGERARSTVNLIRALGGGW 493 Query: 462 DAPRQQ 467 P +Q Sbjct: 494 GVPAEQ 499 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 129 bits (325), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 139/471 (29%), Positives = 214/471 (45%), Gaps = 34/471 (7%) Query: 11 ALAAALLLSGCALGPTGETPAMPQPVHYAAEATPATLGPQEARQTFVLGQRSVPQWWRAF 70 AL A +L GC+L PT E P P Y A+A G E Q LG W + F Sbjct: 18 ALVMAGVLGGCSLAPTYERPQAPVESSYPADAA----GRAEL-QAVNLG------WRQFF 66 Query: 71 GSADLSAWVEEALRSNHSLAEADRNLASARLQLR-AQIDESLWPTIDAVGQAQRQRALGL 129 L + AL +N L A N+ AR Q + A+ D L P ++ G A R R Sbjct: 67 PDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARAD--LLPNLNLSGNAARTRTAAS 124 Query: 130 PNLG--PT-TNLYNVFAGQIRASYTFDLFG-VERFANSALAAQVDAQSPPPPALLGALPQ 185 +L PT +N Y V G +Y D FG V ++ALA+ + + A + + Q Sbjct: 125 QSLTGQPTISNSYQV--GLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQ 182 Query: 186 VADT---ERLVA----LAD-------ADVGELQRRLALGAVSNDDVLSARANAQSLRASL 231 VA ER A LAD +++ Q+RL +GA + D + +S R + Sbjct: 183 VAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVESARVAK 242 Query: 232 PALKAQWQGARHALAVLMGRTPDAAPADLDITRLKLPEQVPMTVPSELLKQRPDILAADA 291 L+ Q A +AL +L+G+ P ++ K+ +P +PS+LL RPDI +A+ Sbjct: 243 TQLERQRAQAENALTLLVGKKIGELPPAQLLSDEKIVTDIPAGLPSDLLTNRPDIRSAEQ 302 Query: 292 ALKVAAAQQALATAEMFPSLSISASFGQSGFSWAKATSGAGAVWGLGASLTQPLFHGGAL 351 +L A A A A FP +S++ +FG + + W G SL+ P+F G Sbjct: 303 SLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFDFGRN 362 Query: 352 RAKRDASKEAYLAAEAHYRQTVLNAFQNVADSLVALTQDADALQASAAARAATEQGWRNA 411 A D ++ A Y + + AF+ VAD+LVA + ++A A AA ++ + Sbjct: 363 SANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAAQQERLKLT 422 Query: 412 QRRAQLGAYPASAARAGERQYLNARLGEIRATASRMNDTATLYQAMGVGLD 462 R + G + +R +A+ I+A R+N+ LY+A+G GL+ Sbjct: 423 DLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLN 473 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 125 bits (313), Expect = 9e-30, Method: Compositional matrix adjust. Identities = 137/464 (29%), Positives = 203/464 (43%), Gaps = 40/464 (8%) Query: 20 GCALGPTGETPAMPQPVHYAAEATPATLGPQEARQTFVLGQRSVPQ--WWRAFGSADLSA 77 GCA GP P + P + +EA ++ P +A P+ WW FG A L+A Sbjct: 18 GCASGPDYTRPQVDMPAAWTSEAPWRSMQPADA----------TPRGPWWERFGDAQLNA 67 Query: 78 WVEEALRSNHSLAEADRNLASARLQLRAQIDESLWPTIDAVGQAQRQRA--------LGL 129 ++AL N +LA A LA AR QL + P ++ +A R R Sbjct: 68 LQQQALAGNQTLAIATARLAQARAQLNV-TGAAQTPQVNLNARAARSRISANRPLTNYNS 126 Query: 130 PNLGPTTNLYNVFAGQIRASYTFDLFG-VERFANSALAAQVDAQSPPPPALLGALPQVAD 188 PN + + N FA SY D FG V+R +A AA + + L ++A Sbjct: 127 PNF---STVQNDFALGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAELAT 183 Query: 189 TERLVALADADVGELQRRLAL--------------GAVSNDDVLSARANAQSLRASLPAL 234 + D ++ ++R +AL GA S D+ +A S + L Sbjct: 184 NYFNLRELDVELDVVRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQIDLL 243 Query: 235 KAQWQGARHALAVLMGRTPDAAPADLDITRLKLPEQVPMTVPSELLKQRPDILAADAALK 294 Q HA+A L G A +T + P +P+ VPS++L++RPDI AA+ A+ Sbjct: 244 GRQRAQYEHAIATLTGTPAPAFSITPSLTPIT-PPAIPVGVPSDVLERRPDIAAAERAMA 302 Query: 295 VAAAQQALATAEMFPSLSISASFGQSGFSWAKATSGAGAVWGLGASLTQPLFHGGALRAK 354 A AQ +A+A +PS + S+G +WA + +W LG S +Q LF GG LRA Sbjct: 303 AANAQIGVASAAYYPSFMLQPSYGVDSRNWATLFNAPSLLWSLGVSASQSLFDGGRLRAG 362 Query: 355 RDASKEAYLAAEAHYRQTVLNAFQNVADSLVALTQDADALQASAAARAATEQGWRNAQRR 414 D S+ Y A A YR+TVL A Q V D + L A S A A+ + A R Sbjct: 363 VDFSQAGYEATVASYRRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLEIASSR 422 Query: 415 AQLGAYPASAARAGERQYLNARLGEIRATASRMNDTATLYQAMG 458 + GA P ++ LN+ + R+ + L +A+G Sbjct: 423 YEGGATPYLDVITAQQSLLNSERQAAQLMGQRLLVSVFLIKALG 466 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.127 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,296,465 Number of extensions: 49455 Number of successful extensions: 296 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 258 Number of HSP's successfully gapped: 17 Length of query: 471 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 382 Effective length of database: 1,198,455 Effective search space: 457809810 Effective search space used: 457809810 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)