BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0030.p (469 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311305|ref|YP_003775397.1| glucosyltransferase involved in... 755 0.0 gi|300312044|ref|YP_003776136.1| lipopolysaccharide synthesis su... 137 3e-33 gi|300313506|ref|YP_003777598.1| galactosyltransferase [Herbaspi... 120 2e-28 >gi|300311305|ref|YP_003775397.1| glucosyltransferase involved in lipopolysaccharide synthesis protein [Herbaspirillum seropedicae SmR1] Length = 441 Score = 755 bits (1950), Expect = 0.0, Method: Compositional matrix adjust. Identities = 369/441 (83%), Positives = 406/441 (92%), Gaps = 1/441 (0%) Query: 30 LIVAYLWVLVRLHGHAFNGDYWVLAIMAFFLSSYFFTEFHERRLRRNPKGPSIISPV-LD 88 +IVAYLWVLVRLHG F+GDYW+LAIMAFF+SSYFFTEFHERRLRRNPKG ++ + +D Sbjct: 1 MIVAYLWVLVRLHGVPFSGDYWMLAIMAFFISSYFFTEFHERRLRRNPKGTRPVASIFVD 60 Query: 89 WLAVAAVLVVVAYICEFYQQFSLRAIFFWVVTTPVGLIISQQLQSRVMRDLHSKGEVRKA 148 WLAV AVL ++ Y+CEFYQQF+ + WV+ TPVGL++SQ QSRVMRDLH+KGEV KA Sbjct: 61 WLAVVAVLGLIGYVCEFYQQFAPGVLAAWVIGTPVGLLVSQYAQSRVMRDLHTKGEVSKA 120 Query: 149 IIIGVNPAALKLADRVEKYPSLMIKLMGFFDDRELNRQPAGTFTPLHGKMSDVAAYVREN 208 IIIGVNPAALKLA+R++ YP+LMIKLMGFFDDRE+ RQP GTFTPL GKMSDVAAYVR++ Sbjct: 121 IIIGVNPAALKLAERMDNYPALMIKLMGFFDDREIGRQPQGTFTPLMGKMSDVAAYVRKH 180 Query: 209 NINMVYISMPISAQPRVLQMIDELQDTTASIYFIPDIYIFNLIQARFDYVGGMAVMAICE 268 NINMVYISMPISAQPRVLQMIDELQDTTASIYF+PDIYIFNLIQARFDYVGGMAVMAICE Sbjct: 181 NINMVYISMPISAQPRVLQMIDELQDTTASIYFVPDIYIFNLIQARFDYVGGMAVMAICE 240 Query: 269 TPFTGTNNIVKRISDLVLASGILLMLLPVMMVIALCVKLTSPGPIIFKQRRYGLDGEEIV 328 TPFTG NN VKR+SD++LA+ ILLMLLPV++VIA+CVK TSPGP+IFKQRRYGLDGEEIV Sbjct: 241 TPFTGMNNFVKRVSDILLATVILLMLLPVLVVIAICVKATSPGPVIFKQRRYGLDGEEIV 300 Query: 329 VYKFRSMTVMEDGGKVTQATKGDMRLTKIGGFLRSSSLDELPQFFNVLQGRMSIVGPRPH 388 VYKFRSMTV+EDG V QATKGD RLTKIGGFLR SSLDELPQF NVLQGRMSIVGPRPH Sbjct: 301 VYKFRSMTVLEDGANVQQATKGDARLTKIGGFLRKSSLDELPQFVNVLQGRMSIVGPRPH 360 Query: 389 AVAHNELYRKQIKGYMLRHKVKPGITGWAQVNGLRGETETLDKMKARIEFDLEYLRRWSL 448 AVAHNELYRKQIKGYMLRHKVKPGITGWAQVNGLRGETETLDKMKARIEFDLEYLRRWSL Sbjct: 361 AVAHNELYRKQIKGYMLRHKVKPGITGWAQVNGLRGETETLDKMKARIEFDLEYLRRWSL 420 Query: 449 MFDLWIIFQTVRMVIKRDNAY 469 FDLWII QTVR+V+KR+NAY Sbjct: 421 TFDLWIIVQTVRLVLKRENAY 441 >gi|300312044|ref|YP_003776136.1| lipopolysaccharide synthesis sugar transferase [Herbaspirillum seropedicae SmR1] Length = 461 Score = 137 bits (344), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 78/186 (41%), Positives = 115/186 (61%), Gaps = 3/186 (1%) Query: 279 KRISDLVLASGILLMLLPVMMVIALCVKLTSPGPIIFKQRRYGLDGEEIVVYKFRSMTV- 337 KR DL+ + +L +L P+M+ A+C+ L GP++++Q R G + E +V KFRSM V Sbjct: 273 KRSFDLITSCIMLPLLSPLMLGAAICIWLEDRGPVLYRQERVGKNNEHFMVLKFRSMRVD 332 Query: 338 MEDGGKVTQATKGDMRLTKIGGFLRSSSLDELPQFFNVLQGRMSIVGPRPHAVAHNELYR 397 E G A+ GD R+T++G +R +DELPQ FNVL+G MS VGPRP E + Sbjct: 333 AEKAGTPQWASSGDPRITRVGNVMRKYRIDELPQLFNVLRGDMSFVGPRPERPYFVEQFA 392 Query: 398 KQIKGYMLRHKVKPGITGWAQVNGLRGETETLDKMKARIEFDLEYLRRWSLMFDLWIIFQ 457 ++I Y +RH VKPGITGWAQV G ++ D ++++DL Y++ SL D+ I+ Sbjct: 393 REITFYNVRHCVKPGITGWAQVRYKYGASK--DDAVQKLQYDLYYVKNNSLFLDVLILID 450 Query: 458 TVRMVI 463 T+ +VI Sbjct: 451 TLSVVI 456 >gi|300313506|ref|YP_003777598.1| galactosyltransferase [Herbaspirillum seropedicae SmR1] Length = 186 Score = 120 bits (302), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 75/189 (39%), Positives = 108/189 (57%), Gaps = 9/189 (4%) Query: 278 VKRISDLVLA-SGILLMLLPVMMVIALCVKLTSPGPIIFKQRRYGLDGEEIVVYKFRSMT 336 +KR+ D +A +L+ +P++MV+ L V+LTSPGP ++ R G + KFRSM Sbjct: 1 MKRLFDFAVALVASVLLAIPIVMVM-LAVRLTSPGPALYWSERVGRHNRIFKMPKFRSMR 59 Query: 337 VMEDGGKVTQATKGDMRLTKIGGFLRSSSLDELPQFFNVLQGRMSIVGPRPHAVAHNELY 396 + LT IG FLR SSLDELPQ +++L+G MS+VGPRP ++L Sbjct: 60 IDTPAVATHLLEDPAQWLTPIGSFLRKSSLDELPQLWSILRGDMSLVGPRPALFNQDDLI 119 Query: 397 RKQIKGYMLRHKVKPGITGWAQVNGLRGETETLDKMKARIEFDLEYLRRWSLMFDLWIIF 456 ++ H++ PG+TGWAQ+NG R E DK + D EYL+R S +FDL I++ Sbjct: 120 --TLRTSQGVHELVPGLTGWAQING-RDELPIPDK----VRLDAEYLQRRSFLFDLKILW 172 Query: 457 QTVRMVIKR 465 T V+ R Sbjct: 173 MTALKVLAR 181 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.141 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,337,096 Number of extensions: 51169 Number of successful extensions: 144 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 139 Number of HSP's successfully gapped: 3 Length of query: 469 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 380 Effective length of database: 1,198,455 Effective search space: 455412900 Effective search space used: 455412900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)