BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0545.p (424 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312458|ref|YP_003776550.1| methyl-accepting chemotaxis sen... 721 0.0 gi|300310309|ref|YP_003774401.1| methyl-accepting chemotaxis tra... 347 1e-96 gi|300313825|ref|YP_003777917.1| methyl-accepting chemotaxis tra... 283 2e-77 gi|300309601|ref|YP_003773693.1| methyl-accepting chemotaxis tra... 253 2e-68 gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis tra... 100 2e-22 >gi|300312458|ref|YP_003776550.1| methyl-accepting chemotaxis sensory transducer protein [Herbaspirillum seropedicae SmR1] Length = 684 Score = 721 bits (1862), Expect = 0.0, Method: Compositional matrix adjust. Identities = 359/390 (92%), Positives = 377/390 (96%) Query: 1 MLSSLRARLIVICVLIVAVAMIVLSGANIYTVRSDTLEGISTQTRQLTESYAANITEWVR 60 MLSSLRARLI ICVLIVA+AMI+LS ANI+TVRSDTLE IS+QT+QLTES+AANI+EWVR Sbjct: 1 MLSSLRARLIFICVLIVALAMIILSAANIFTVRSDTLEAISSQTQQLTESHAANISEWVR 60 Query: 61 SKRIITGAMKQALKQSDVLPIVAAAQEAGSFDDAYIGYPDKRMLALHPMPAGYDPTARPW 120 SKR++TGAMKQALKQSDVLPIVAAAQEAGSFDDAYIGYPDKRMLALHPMPAGYDPTARPW Sbjct: 61 SKRVVTGAMKQALKQSDVLPIVAAAQEAGSFDDAYIGYPDKRMLALHPMPAGYDPTARPW 120 Query: 121 YKQAVDAGKPVLTAPYVDATTGKLVVTFAEPVSDKGALQAVLGSDVQLDNVVRTVGGIKP 180 YKQAV+AGKPVLTAPYVDATTGKLVVTFAEPV DK LQAVLGSDVQLDNVVRTV GIKP Sbjct: 121 YKQAVEAGKPVLTAPYVDATTGKLVVTFAEPVGDKSNLQAVLGSDVQLDNVVRTVAGIKP 180 Query: 181 TPSSFAFIVGKDGLIITHPNKELALKPVSALDQNLSAASLAGMKQGGSATIGGVSYLLFT 240 TPSSFAFIVGKDG+IITHPNKELALKPVS+LDQ LSA SLAGMKQGGSATIGG+ YLLFT Sbjct: 181 TPSSFAFIVGKDGVIITHPNKELALKPVSSLDQTLSAQSLAGMKQGGSATIGGIRYLLFT 240 Query: 241 QPIDNTDWSLVVALDYREATHAIGALMALAVILAVVAIVAAALLLSYTITRLLRRLGVVR 300 QPI NTDWSLVVALDYREATHAIGALM L+ +LAV+AIVA+A+LLSYTITRLLRRLGVVR Sbjct: 241 QPIANTDWSLVVALDYREATHAIGALMTLSTVLAVLAIVASAILLSYTITRLLRRLGVVR 300 Query: 301 NAMEEIASGDGDLTRRLDTSGKDELAQISRAFNHFADKISRTLLDIRRASESVKVSSSEI 360 NAMEEIASGDGDLTRRLDTSGKDELAQISRAFNHFADKISRTLLDIRRASESVKVSSSEI Sbjct: 301 NAMEEIASGDGDLTRRLDTSGKDELAQISRAFNHFADKISRTLLDIRRASESVKVSSSEI 360 Query: 361 ASGNMDLSARTEQQAGSLEETASAMEELTS 390 ASGNMDLSARTEQQAGSLEETASAMEELTS Sbjct: 361 ASGNMDLSARTEQQAGSLEETASAMEELTS 390 >gi|300310309|ref|YP_003774401.1| methyl-accepting chemotaxis transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 625 Score = 347 bits (889), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 198/396 (50%), Positives = 256/396 (64%), Gaps = 10/396 (2%) Query: 3 SSLRARLIVICVLIVAVAMIVLSGANIYTVRSDTLEGISTQTRQLTESYAANITEWVRSK 62 +SLR RLI +C IV +AM+ + N T RS T+ + TQ QL +S AA I EW SK Sbjct: 23 TSLRTRLIALCAGIVVLAMLAIVLTNFLTARSRTMSTLDTQMTQLAQSQAAAIAEWSDSK 82 Query: 63 RIITGAMKQALKQSDVLPIVAAAQEAGSFDDAYIGYPDKRMLALHPMPAG--YDPTARPW 120 +++ ++K+A +D LP V AAQ AG FD AY+GY DK + YDPT RPW Sbjct: 83 KLVVTSIKEAADAADPLPFVKAAQTAGGFDLAYLGYADKHAVFSQQRSRAPDYDPTKRPW 142 Query: 121 YKQAVDAGKPVLTAPYVDATTGKLVVTFAEPVSDKGALQAVLGSDVQLDNVVRTVGGIKP 180 Y+QA +PVLTAPY A+TGKL+VTFA+ VS G + AV +DV LD VV V IKP Sbjct: 143 YQQAAKTSEPVLTAPYTSASTGKLLVTFAQSVSKDGQVAAVAAADVLLDTVVADVVAIKP 202 Query: 181 TPSSFAFIVGKDGLIITHPNKELALKPVSALD-----QNLSAASLAGMKQGGSATIGGVS 235 TP+S+AF+ G II HPN +L LKP++ LD Q L A G+ Q + G Sbjct: 203 TPNSYAFLADLSGKIIAHPNDKLRLKPLADLDAAITPQTLDEAMRTGVHQ--DVALDGRR 260 Query: 236 YLLFTQPIDNTDWSLVVALDYREATHAIGALMALAVILAVVAIVAAALLLSYTITRLLRR 295 +LF + TDW LVVALD +A ++ A++ + ++AV+ V AAL+L I+RLL R Sbjct: 261 QMLFVARVGGTDWLLVVALDRADALASLSAMLVSSGVMAVLVTVLAALVLGALISRLLGR 320 Query: 296 LGVVRNAMEEIASGDGDLTRRLDTSGK-DELAQISRAFNHFADKISRTLLDIRRASESVK 354 L VV+ A+EEIA GDGDLTRRLDT G+ DEL +I AFN F DKI ++DIR AS ++ Sbjct: 321 LTVVQGALEEIADGDGDLTRRLDTHGRSDELGRIGAAFNRFVDKIEHVMIDIRDASHTIH 380 Query: 355 VSSSEIASGNMDLSARTEQQAGSLEETASAMEELTS 390 +S +IASGNMDLS RTE QA SLE+TA+AME+LTS Sbjct: 381 AASRDIASGNMDLSQRTESQASSLEQTAAAMEQLTS 416 >gi|300313825|ref|YP_003777917.1| methyl-accepting chemotaxis transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 598 Score = 283 bits (725), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 158/395 (40%), Positives = 238/395 (60%), Gaps = 5/395 (1%) Query: 1 MLSSLRARLIVICVLIVAVAMIVLSGANIYTVRSDTLEGISTQTRQLTESYAANITEWVR 60 M +S++AR++ + ++ ++ V+ + + ++ D I + + + I EWV Sbjct: 1 MFNSIKARILFYTITLLIASLAVVGTVSFFIIKLDNDRAIERNAESVASGFGSTINEWVA 60 Query: 61 SKRIITGAMKQALKQSDVLPIVAAAQEAGSFDDAYIGYPDKRML--ALHPMPAGYDPTAR 118 ++ +T A + +D + ++ Q++G F +G DK A +PAGYDPTAR Sbjct: 61 ARTAMTVAAADGVLNADPVFVIKTLQKSGGFYVTTLGKEDKTAFTSAAEGLPAGYDPTAR 120 Query: 119 PWYKQAVDAGKPVLTAPYVDATTGKLVVTFAEPVSDKGALQAVLGSDVQLDNVVRTVGGI 178 PWYKQAV AGKPVLT PY D T K +V+F PV G V+ + + LD V V I Sbjct: 121 PWYKQAVAAGKPVLTKPYTDVATKKPMVSFTAPVMQNGQRAGVVAAAMFLDGVSDVVRSI 180 Query: 179 KPTPSSFAFIVGKDGLIITHPNKELALKPVSALDQNLSAA---SLAGMKQGGSATIGGVS 235 PTP+SFAF+V +DGL++ HP+ + KP + L+ +LA + + G + Sbjct: 181 HPTPASFAFLVDRDGLMLAHPDGQWVNKPAKEWNAELTPERLNALAADDKASEVMLEGAA 240 Query: 236 YLLFTQPIDNTDWSLVVALDYREATHAIGALMALAVILAVVAIVAAALLLSYTITRLLRR 295 L+ PI T+W+L++A+D + T + A+I V+ + AA ++S T + +R Sbjct: 241 KLVKGVPIKGTEWTLMLAMDKADVTAGLRNASRSALIALVLVALVAAGIMSLITTAVFQR 300 Query: 296 LGVVRNAMEEIASGDGDLTRRLDTSGKDELAQISRAFNHFADKISRTLLDIRRASESVKV 355 L VR AM+ ++SG+GDLT RL G DE+ QISR+FN F ++I+ L D+RR+S++VK+ Sbjct: 301 LSRVRRAMDNLSSGNGDLTLRLTEDGSDEVTQISRSFNTFVNQITGILQDVRRSSDTVKI 360 Query: 356 SSSEIASGNMDLSARTEQQAGSLEETASAMEELTS 390 SS+EIA+GN DLSARTEQQAG+LEETASAMEELTS Sbjct: 361 SSTEIATGNQDLSARTEQQAGALEETASAMEELTS 395 >gi|300309601|ref|YP_003773693.1| methyl-accepting chemotaxis transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 253 bits (647), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 157/395 (39%), Positives = 228/395 (57%), Gaps = 7/395 (1%) Query: 1 MLSSLRARLIVICVLIVAVAMIVLSGANIYTVRSDTLEGISTQTRQLTESYAANITEWVR 60 MLSSLRAR+I +L+V ++++ + AN R + +AA I +W+ Sbjct: 1 MLSSLRARVICALILLVISSVLISTAANYLIARKGLNAATDDDLATAVDQHAAVIGDWLA 60 Query: 61 SKRIITGAMKQALKQSDVLPIVAAAQEAGSFDDAYIGYPDKRMLALH--PMPAGYDPTAR 118 K + +++ SD ++ Q AG F D +GYPDKR +PA YDPT R Sbjct: 61 GKSKMIASLEDISLTSDPDAMLKQIQVAGGFYDVGVGYPDKRAKFTDWPNIPATYDPTTR 120 Query: 119 PWYKQAVDAGKPVLTAPYVDATTGKLVVTFAEPVSDKGALQAVLGSDVQLDNVVRTVGGI 178 PWYK + AGKPV PYV +T+G L+V A PV G L+AV+ DV LD+VV + I Sbjct: 121 PWYKGVLQAGKPV-ALPYV-STSGLLLVALARPVMRDGVLKAVVVGDVALDSVVENIKSI 178 Query: 179 KPTPSSFAFIVGKDGLIITHPNKELALKPVSALDQNLSAASLAGMKQGG---SATIGGVS 235 PTPSSF +V + G II HP+ +L K S L S ++A G + G Sbjct: 179 HPTPSSFGMLVEQGGRIIAHPDAQLRFKQASELAPEFSDLNVANAGPGQPPVHIVVDGRE 238 Query: 236 YLLFTQPIDNTDWSLVVALDYREATHAIGALMALAVILAVVAIVAAALLLSYTITRLLRR 295 L+ Q + TDW++VVA+D +EA+ + AL+A +++ V ++AA+L+ + RR Sbjct: 239 KLVRVQAVPGTDWNIVVAIDQQEASAGVRALLASSLLSLVAVVIAASLIGAAVTATAFRR 298 Query: 296 LGVVRNAMEEIASGDGDLTRRLDTSGKDELAQISRAFNHFADKISRTLLDIRRASESVKV 355 LG + AM I SG GDLT+RL G+DE+A I+++FN F K+ + DIR A E+V+V Sbjct: 299 LGQISAAMTAIGSGSGDLTQRLPDHGRDEVAIIAQSFNAFVAKLQSVMRDIRDAGETVRV 358 Query: 356 SSSEIASGNMDLSARTEQQAGSLEETASAMEELTS 390 +S EIA+ N DLS RTE A SLE+TA++M E+++ Sbjct: 359 ASDEIAAANHDLSVRTESAAASLEQTAASMAEISA 393 >gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 581 Score = 100 bits (249), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 60/126 (47%), Positives = 83/126 (65%), Gaps = 4/126 (3%) Query: 267 MALAVILAV-VAIVAAALLLSYTITR-LLRRLGVVRNAMEEIASGDGDLTRRLDTSGKDE 324 MA+ V LA +A V AA++L Y + R +++ L E +A+GD LT R++ +DE Sbjct: 190 MAIEVTLACSMAAVLAAIILGYLMARSIVKPLTEAVRVAENVAAGD--LTTRIEPHSRDE 247 Query: 325 LAQISRAFNHFADKISRTLLDIRRASESVKVSSSEIASGNMDLSARTEQQAGSLEETASA 384 Q+ +A D + + +RR +ES+ +S EIASGN+DLSARTEQQAGSLEETASA Sbjct: 248 TGQLMQALRKMNDNLIEIVSGVRRGTESITTASGEIASGNLDLSARTEQQAGSLEETASA 307 Query: 385 MEELTS 390 MEE+TS Sbjct: 308 MEEMTS 313 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.131 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,184,061 Number of extensions: 48254 Number of successful extensions: 328 Number of sequences better than 1.0e-08: 60 Number of HSP's gapped: 301 Number of HSP's successfully gapped: 60 Length of query: 424 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 336 Effective length of database: 1,203,190 Effective search space: 404271840 Effective search space used: 404271840 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)