BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0531.p (399 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312475|ref|YP_003776567.1| metal-dependent amidase/aminoac... 748 0.0 gi|300310161|ref|YP_003774253.1| aminohydrolase [Herbaspirillum ... 370 e-103 gi|300310472|ref|YP_003774564.1| hippurate hydrolase [Herbaspiri... 337 7e-94 >gi|300312475|ref|YP_003776567.1| metal-dependent amidase/aminoacylase/carboxypeptidase [Herbaspirillum seropedicae SmR1] Length = 399 Score = 748 bits (1931), Expect = 0.0, Method: Compositional matrix adjust. Identities = 357/397 (89%), Positives = 382/397 (96%) Query: 1 MSHPHEHALCEVADLHDIHDEITGIRRHLHQHPELSFEEVDTAALVASRLEEWGYAVTRH 60 MSH H LCEV DLHDI DEIT IRRHLHQHPELSFEEVDTAALVA RLE+WGYAVTRH Sbjct: 1 MSHAHPGHLCEVGDLHDIQDEITAIRRHLHQHPELSFEEVDTAALVAQRLEQWGYAVTRH 60 Query: 61 IGGNGLVATLKAGTSARSIGLRADMDALPIHEETGLDWASVNPGAMHACGHDGHTAMLLG 120 IGGNGLVATL+ G+SARSIGLRADMDALPI EETGLDWASV PGAMHACGHDGHTAMLLG Sbjct: 61 IGGNGLVATLRVGSSARSIGLRADMDALPIQEETGLDWASVKPGAMHACGHDGHTAMLLG 120 Query: 121 AAKHLARTRRFDGTLHLIFQPAEEAGFDSGAQKMLADGLFERFPCDAVFGIHNHPGVEAG 180 AA+HLARTRRFDGTL+LIFQPAEEAGFDSGAQKMLADGLFERFPC+AVFGIHNHPGVEAG Sbjct: 121 AARHLARTRRFDGTLNLIFQPAEEAGFDSGAQKMLADGLFERFPCEAVFGIHNHPGVEAG 180 Query: 181 TFMFRSGPFMAACDTVKIRLTGRGSHAARPHLSVDPVVAAASLVMALQTVVSRNVDPMET 240 TFMFRSGPFMAACDTVKIR+TGRGSHAARPHLSVDPVV AASLVMALQTVVSRN+DPM++ Sbjct: 181 TFMFRSGPFMAACDTVKIRITGRGSHAARPHLSVDPVVVAASLVMALQTVVSRNIDPMDS 240 Query: 241 AVVTVGSLHAGKASNVIPEFATLELSVRSFKPEVRALLEERIRALVALHAESYGARADIE 300 AVVTVGSLHAGKASNVIPEFAT+ELSVRSFKPEVR LLE+RIRALV+ HA+SYGA+A+I+ Sbjct: 241 AVVTVGSLHAGKASNVIPEFATMELSVRSFKPEVRELLEQRIRALVSTHAQSYGAQAEID 300 Query: 301 YLRGYPVLVNSDAETEFARSVAEELVGAEKVIAPFGPIAGSEDFAYFLQQRPGCFLRVGN 360 YLRGYPVLVNSDAET+FARSVAEELVG EKVIAPFGPIAGSEDFAYFL+QRPGCFLRVGN Sbjct: 301 YLRGYPVLVNSDAETDFARSVAEELVGPEKVIAPFGPIAGSEDFAYFLRQRPGCFLRVGN 360 Query: 361 GQGQPMLHNAGYDFNDANIPVGAAFWTRLVERWLPVK 397 GQG+PMLHNAGYDFNDANIP+GAA+WTRLVERW+P++ Sbjct: 361 GQGKPMLHNAGYDFNDANIPIGAAYWTRLVERWMPLQ 397 >gi|300310161|ref|YP_003774253.1| aminohydrolase [Herbaspirillum seropedicae SmR1] Length = 402 Score = 370 bits (950), Expect = e-103, Method: Compositional matrix adjust. Identities = 192/389 (49%), Positives = 247/389 (63%), Gaps = 13/389 (3%) Query: 21 EITGIRRHLHQHPELSFEEVDTAALVASRLEEWGYAVTRHIGGNGLVATLKAGTSARSIG 80 ++ IRRHLH HPEL FEE TA+ VA L +G V +IGG G+VATL+ G S R+I Sbjct: 16 DLVSIRRHLHAHPELRFEEKRTASFVADMLRSYGLTVAENIGGYGVVATLRKGKSTRAIA 75 Query: 81 LRADMDALPIHEETGLDWASVNPGAMHACGHDGHTAMLLGAAKHLARTRRFDGTLHLIFQ 140 LRADMDALP+ E+ S G MHACGHDGHT MLLGAA+ L+R FDGT+H +FQ Sbjct: 76 LRADMDALPMSEQNDFSHISTCAGKMHACGHDGHTTMLLGAARRLSREVEFDGTVHFVFQ 135 Query: 141 PAEEAGFDSGAQKMLADGLFERFPCDAVFGIHNHPGVEAGTFMFRSGPFMAACDTVKIRL 200 PAEE G +GA+ M+ DGLFERFP DA+FG+HN PG+ AG+F R GP MA+ +T L Sbjct: 136 PAEEGG--AGARLMIEDGLFERFPADAIFGVHNWPGLPAGSFGLRPGPLMASSNTFSATL 193 Query: 201 TGRGSHAARPHLSVDPVVAAASLVMALQTVVSRNVDPMETAVVTVGSLHAGKASNVIPEF 260 GRG+H A+PH S+DPV+AAA L +A Q++V+RN++P AV++V LH G A NVIPE Sbjct: 194 FGRGAHGAQPHRSIDPVLAAAQLTLAWQSIVTRNINPNHRAVISVTQLHTGTADNVIPEQ 253 Query: 261 ATLELSVRSFKPEVRALLEERIRALVALHAESYGARADIEYLRGYPVLVNSDAETEFARS 320 ATL +VRSF E L+E R+ A+ + + R + R YP +VN T+ A Sbjct: 254 ATLSGTVRSFDAETLDLIEHRMAAIAEAIGQMFDLRVQFHFERLYPAVVNHPEATQQAMQ 313 Query: 321 VAEELVGAEKVIAPFGPIAGSEDFAYFLQQRPGCFLRVGNG---QGQPM--------LHN 369 VGAE+V P SEDFA++LQ +PGC+ +GNG G+ LH+ Sbjct: 314 AMMAAVGAEQVDGDTEPAFASEDFAFYLQHKPGCYAFLGNGVIADGKQAGSQATSRELHS 373 Query: 370 AGYDFNDANIPVGAAFWTRLVERWLPVKA 398 YDFND I G A+W LV ++L V A Sbjct: 374 PFYDFNDDIIDAGVAYWVSLVRQYLGVAA 402 >gi|300310472|ref|YP_003774564.1| hippurate hydrolase [Herbaspirillum seropedicae SmR1] Length = 403 Score = 337 bits (865), Expect = 7e-94, Method: Compositional matrix adjust. Identities = 173/392 (44%), Positives = 241/392 (61%), Gaps = 5/392 (1%) Query: 6 EHALCEVADLHDIHDEITGIRRHLHQHPELSFEEVDTAALVASRLEEWGYAVTRHIGGNG 65 EH + LH G+RR LH HPEL FEE TA +V L+ GY VTR +GG G Sbjct: 2 EHTGHQHDTLHQHRAHWAGLRRDLHAHPELRFEEHRTADVVVRELQSLGYTVTRGLGGTG 61 Query: 66 LVATLKAGTSARSIGLRADMDALPIHEETGLDWASVNPGAMHACGHDGHTAMLLGAAKHL 125 +VA+L R I LRAD+DALPI E AS G MHACGHDGHT MLLGAA+ L Sbjct: 62 VVASLAGADPQRGIVLRADLDALPIVEANDFAHASCTHGVMHACGHDGHTVMLLGAARLL 121 Query: 126 ARTRRFDGTLHLIFQPAEEAGFDSGAQKMLADGLFERFPCDAVFGIHNHPGVEAGTFMFR 185 + G++H +FQP EE G +GA+KM+ DGLFE+FP +AVFG+HN PG+ AG F R Sbjct: 122 KQGPMLPGSVHFVFQPGEEGG--AGARKMIDDGLFEQFPTEAVFGMHNWPGLPAGQFGLR 179 Query: 186 SGPFMAACDTVKIRLTGRGSHAARPHLSVDPVVAAASLVMALQTVVSRNVDPMETAVVTV 245 GP MAA +KI +TG+G+HAA+PHL +DP+ A S+V+ QT+ +R+ DP++ AV++V Sbjct: 180 PGPIMAAGSRLKITITGKGAHAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISV 239 Query: 246 GSLHAGKASNVIPEFATLELSVRSFKPEVRALLEERIRALVALHAESYGARADIEYLRGY 305 HAG NVIP+ A L ++R+ ++ L+E IR + AE+YGA+ ++E+ + Y Sbjct: 240 CMFHAGDTDNVIPDRAELRGTIRTLSSTLQQKLQEDIRRMCHALAEAYGAKVEVEFFQYY 299 Query: 306 PVLVNSDAETEFARSVAEELVGAEKVIAPFGPIAGSEDFAYFLQQRPGCFLRVGNG---Q 362 P +N+ AET+F V + G +++ SEDF + L++RPG ++ +GN + Sbjct: 300 PATINTPAETDFCERVIRQTFGDDRIRTGIPANMTSEDFGFMLEERPGAYVLIGNAPQDR 359 Query: 363 GQPMLHNAGYDFNDANIPVGAAFWTRLVERWL 394 LH+ YDFND I G +W L + + Sbjct: 360 ASHSLHHPHYDFNDDIIEAGVRYWVALAQGYF 391 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,353,799 Number of extensions: 60717 Number of successful extensions: 166 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 162 Number of HSP's successfully gapped: 3 Length of query: 399 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 312 Effective length of database: 1,207,925 Effective search space: 376872600 Effective search space used: 376872600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)