BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0523.p (1372 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312484|ref|YP_003776576.1| serine endopeptidase [Herbaspir... 1834 0.0 >gi|300312484|ref|YP_003776576.1| serine endopeptidase [Herbaspirillum seropedicae SmR1] Length = 1371 Score = 1834 bits (4751), Expect = 0.0, Method: Compositional matrix adjust. Identities = 974/1385 (70%), Positives = 1132/1385 (81%), Gaps = 27/1385 (1%) Query: 1 MKNKKKQGKSACIAPPAGLRQHRLCAAVLLTLGPALSLPAAAQSFVNYDGSTTTDAAAAL 60 MK +K ++A + A R+ L AAVLLTLGPAL PA+AQS+VNYDG++T DA+AAL Sbjct: 1 MKKSRKSPQNAALTTSAVFRKQGLYAAVLLTLGPALGTPASAQSYVNYDGASTADASAAL 60 Query: 61 ASWTGNKEFQTDWGLGAMNAQYAYAAGFSGLGIKLGAVDSGLLLSHAEFVTRNVKAVAVT 120 A+WTG+ EF+ DWGLGAMNAQYAY AG++G GIKLGAVDSGLLL+H EF RNVKA+AVT Sbjct: 61 ATWTGHAEFKADWGLGAMNAQYAYVAGYTGQGIKLGAVDSGLLLTHNEFARRNVKALAVT 120 Query: 121 GTYANDGSQYQDGSQAWKAGDAFSTTGALSKLNDKHGSHVSGTIAAAKNGAGIMGVAFAS 180 G YANDG QYQDGSQAWKAGDAFS GALS LNDKHGSHVSGTIAAAKNG GIMGVAFAS Sbjct: 121 GAYANDGYQYQDGSQAWKAGDAFSVPGALSPLNDKHGSHVSGTIAAAKNGDGIMGVAFAS 180 Query: 181 DYHITNTNGTDSSVYGVNVDYNYFKAAYGNLANAGVRAINSSWGSPDSRDDFSSIGGVAA 240 DY+ITNTNGTDSSVYGVN+DYNYFKAAYGNLA+AGVRAINSSWG+PDSRDDF SI GVAA Sbjct: 181 DYYITNTNGTDSSVYGVNMDYNYFKAAYGNLASAGVRAINSSWGNPDSRDDFGSISGVAA 240 Query: 241 AYDRLQGGGKKSWLDAAADVAKESNTLMVWAAGNSAKANINIRSALPYFRPELEQNWIAV 300 AY RLQGGGKKSWLDAAADVA+ SNTL+VWAAGN++KAN+NIRSALPYFRPELEQNWIAV Sbjct: 241 AYQRLQGGGKKSWLDAAADVAQASNTLLVWAAGNASKANVNIRSALPYFRPELEQNWIAV 300 Query: 301 AAINASSVLPSFSNQCGLARYWCVSAPGSAITSLNITSDTDLVNASGTSMAAPHVTGALG 360 AA+N SS+LPSFSNQCGLA+YWCVSAPGS ITSLNITSDT LVNASGTSMAAPHVTGALG Sbjct: 301 AALNTSSMLPSFSNQCGLAKYWCVSAPGSGITSLNITSDTALVNASGTSMAAPHVTGALG 360 Query: 361 LLMERYPDLDNQAIRTILLTTAKHLGSGPADVPNAMFGWGVPDLNKALNGPGQLLGVFNA 420 +LM+RYPDLDNQAIRTILL+TAKHLG+G AD PN+MFGWGVPDLNKAL GPGQLLGVF A Sbjct: 361 VLMQRYPDLDNQAIRTILLSTAKHLGTGAADAPNSMFGWGVPDLNKALYGPGQLLGVFKA 420 Query: 421 NIAAGRSDTWSNAISEAALIQRKSDDRAQLAAREAASPDALQAAAASAAVQIASNVESGY 480 NIAAG +DTWSN ISEAAL+QRKS+D+AQLAA +A S LQ+A SA+ Q+ + V+SGY Sbjct: 421 NIAAGSTDTWSNDISEAALVQRKSEDKAQLAAWQALSASTLQSAVDSASAQVKAQVDSGY 480 Query: 481 GQAMALVQTRAQRNATFQASKKKEDNTAFQQADKAVAANPLAAALLKQSGSGPTLSKEEL 540 A+ALVQTRAQ NA +Q+S K+ED TAFQ ADKAVAAN LAAA+LKQSGSG LSK++L Sbjct: 481 DAALALVQTRAQLNAAYQSSNKREDKTAFQNADKAVAANALAAAILKQSGSGALLSKDQL 540 Query: 541 MARLIAADP----ATVALGDYHAQATYLDAARKKTDADYVGSLVKTGEGSLTLSGNNSYS 596 +A ++ D A +ALG+Y+ QA YL AA+KKTDADYVGSLVKTG+GSLTLSGNNSY+ Sbjct: 541 LAYMLTVDSGVAAANLALGNYNGQAVYLAAAQKKTDADYVGSLVKTGDGSLTLSGNNSYT 600 Query: 597 GGTRLEGGTLGVASSTALGTGPLAMSDGTTLRAAADQLAMGNVVSVSGEGRFDTQAFTLT 656 GGTRLEGGTLGV SSTALGTG LAMSDG TLRAAAD LA+ N VS++G GR DTQAF LT Sbjct: 601 GGTRLEGGTLGVGSSTALGTGGLAMSDGVTLRAAADNLALNNAVSINGAGRIDTQAFNLT 660 Query: 657 LNAGIADGRSKGSLVKQWEGTLHVLGPLSYSGNTTIAAGTLSVPAFSLTADQTLTVGVAS 716 LN GIADGR GSLVKQ EGTL VLG +SYSGNTTIAAGTLS+P + TA Q LTVGVAS Sbjct: 661 LNNGIADGRVPGSLVKQGEGTLRVLGTVSYSGNTTIAAGTLSLPTYVQTAGQILTVGVAS 720 Query: 717 ASQYGKLAVTGAASFAPDAHLAVDVYKTASLGSGKTLAGVVTAGSLTAPRLNVTDNSLLF 776 A+QYGKLAVTGAASFA DA LAVDV K A+LG+G+ LAGV+TAGSL+APRL V+DNS+LF Sbjct: 721 ATQYGKLAVTGAASFASDAALAVDVAKAATLGNGQRLAGVITAGSLSAPRLAVSDNSVLF 780 Query: 777 DFKPVVSATALDLNIVDATSIQQV-----AQAVLTPDAAGTTTPAIQ--ATGTQPAATPA 829 DF+PVV++ A+DL+IV A SI +V AQA + A T+TP A+ T A+TP Sbjct: 781 DFQPVVASNAVDLDIVSAVSINKVVLDTLAQAQGPANTATTSTPVASALASSTPVASTPV 840 Query: 830 PPVQPTNTDASSTPASTVQASSTQASTPPAPAVQATSTQASSTPAPAVQATGTPASATPA 889 +NT A+STP +V AS+T A++ P + A++T A+STP + A+ TPA++TP Sbjct: 841 VSAPASNTPAASTPVVSVPASNTPAASTPVVSAPASNTPAASTPVVSAPASNTPAASTPV 900 Query: 890 PAVQATSTQPSTXTQASTPPAPAVQATSTQASTAPAPAVQATTTQASSTQAPTGPAANKP 949 +V P++ T A++ P +V ++T A++ P +V + T +ST + PA+N P Sbjct: 901 VSV------PASNTSAASTPLVSVPVSNTPAASTPVVSVPVSNTPVASTPVVSVPASNTP 954 Query: 950 VPAAPVAPASSPAAPAAPAAASAAPAAAPATPAAASPPPAPAPTVRPSATLIAAATAAAR 1009 + PV AS+PA+ ++ +A A+A A P A P+V AAATAAA Sbjct: 955 AASTPV--ASAPASGTPASSTAAPSASAAPAVVPAVAPAARIPSVV------AAATAAAT 1006 Query: 1010 VAPVLDRQIRQPSTADMAQVVTALGRMPDPESLLRAVSQTLPRDVSASAIRGTMASLNRV 1069 VAP+LDRQI+QPS+ADMAQVVTALGR+PD S+LRA SQTLPR+VS +AIRG +ASL+RV Sbjct: 1007 VAPILDRQIQQPSSADMAQVVTALGRLPDAASVLRAASQTLPRNVSDNAIRGVLASLDRV 1066 Query: 1070 IATRTESGNMGSAA--AGSGLSSGEAGGDRQVWIMPFESRTNQGDRGGESGFSASTSGLA 1127 IATR + GN+ +A GSGL GEAGGDRQVW+MPFESRTNQGDR G+SGFSAST G+A Sbjct: 1067 IATRLDLGNLAGSATGTGSGLGYGEAGGDRQVWLMPFESRTNQGDRDGDSGFSASTWGMA 1126 Query: 1128 AGAEMEFDSARAGLSYAYASTSASGNTAITGTGSRSRIESNMVALYGSLPLGEISLGWQA 1187 GAEM +DS R GLSYAYASTSASGNTAITG GSRSRIESN+VALYGS+PLG+++L WQA Sbjct: 1127 TGAEMAWDSGRVGLSYAYASTSASGNTAITGAGSRSRIESNLVALYGSVPLGKLALSWQA 1186 Query: 1188 DLGRNGNRLDRQMQFGGLNRIASSRFQSWHAHLGTNVSFTLPLSEAMSLVPALQLDYTRL 1247 D G N NRL R+MQFGGL+RIASSR+ +W AHLG NVS TLPLSEA+S+VPALQLDYTR+ Sbjct: 1187 DAGWNSNRLAREMQFGGLHRIASSRYHTWSAHLGANVSHTLPLSEALSVVPALQLDYTRM 1246 Query: 1248 QSPSYAENGAGDLNLSVQGKPSQALLLGTAARLNYALTPGSQISTYLGASYDVINDRDDL 1307 QSPSYAE+GAGDL+LSVQGK +QALLLG RLNYAL+ SQIST++GASYDVINDR DL Sbjct: 1247 QSPSYAESGAGDLSLSVQGKRTQALLLGVGTRLNYALSTTSQISTHVGASYDVINDRGDL 1306 Query: 1308 VATYAGAPGQSFTTSGPARSPWLLKAGVSYRFKLVEAADILLRFDAEGRSGFINQSAAIK 1367 VATYAG PGQ+FT G ARS WL KAGVSYRF+L ADI LR DAEGRSGFINQSAA+K Sbjct: 1307 VATYAGTPGQAFTAPGSARSAWLFKAGVSYRFQLTTEADISLRLDAEGRSGFINQSAALK 1366 Query: 1368 ASWRF 1372 ASWRF Sbjct: 1367 ASWRF 1371 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.311 0.124 0.349 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 3,631,910 Number of extensions: 153163 Number of successful extensions: 607 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 572 Number of HSP's successfully gapped: 10 Length of query: 1372 Length of database: 1,619,870 Length adjustment: 97 Effective length of query: 1275 Effective length of database: 1,160,575 Effective search space: 1479733125 Effective search space used: 1479733125 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 137 (57.4 bits)