BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0522.p (500 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312485|ref|YP_003776577.1| outer membrane protein [Herbasp... 850 0.0 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 176 5e-45 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 165 9e-42 gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 160 3e-40 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 155 6e-39 >gi|300312485|ref|YP_003776577.1| outer membrane protein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 850 bits (2195), Expect = 0.0, Method: Compositional matrix adjust. Identities = 458/498 (91%), Positives = 474/498 (95%), Gaps = 9/498 (1%) Query: 1 MINMDKTSSSFPAVRLIALAAAALKKERRPALLAGCSALGPDYAGPPEAAPQAAHSQQFV 60 MINMDKTSSSFPA RLIALAAAAL ALGPDYAGPPEAAPQAAH QFV Sbjct: 1 MINMDKTSSSFPAARLIALAAAALLA---------GCALGPDYAGPPEAAPQAAHGGQFV 51 Query: 61 RGGDSANSAAPINQWWQGLGDSELNSLIERALQANPNLGVARARLQQSRAGLALEQANAA 120 RGGDSA + APINQWWQGLGD++L SLIERALQANPNLG+ARARLQQSRAGLALEQANAA Sbjct: 52 RGGDSATTTAPINQWWQGLGDAQLTSLIERALQANPNLGIARARLQQSRAGLALEQANAA 111 Query: 121 PSVGASALAGHARLPPANLGALTGGSSSGNGSPTSANLYSVGFDASWEIDLFGARRRAVE 180 PSVGASALAGHARLPPANLGALTGG+SSGNGSPTSANLYS+GFDASWEIDLFG RRRAV+ Sbjct: 112 PSVGASALAGHARLPPANLGALTGGASSGNGSPTSANLYSLGFDASWEIDLFGGRRRAVQ 171 Query: 181 AATATADAAQASLADVQVSLAAEVANAYISLRDRQLRLSLGQEAVQAQEQMLQLTGQRVE 240 AATATADAAQASLADVQVSLAAEVANAYISLRDRQ RL+LGQ+AVQAQEQMLQLTGQRVE Sbjct: 172 AATATADAAQASLADVQVSLAAEVANAYISLRDRQQRLALGQQAVQAQEQMLQLTGQRVE 231 Query: 241 RGTASSLDQLRVQNQLDASRADLVPLQAERDAFLNQLATLLGQEPGTLDSELAATAPVPL 300 RGTASSLDQLR+QNQLDASRADLVPLQA+RDA+LNQLATLLGQEPGTLD ELAAT PVPL Sbjct: 232 RGTASSLDQLRIQNQLDASRADLVPLQADRDAYLNQLATLLGQEPGTLDGELAATVPVPL 291 Query: 301 PPQQVAVGDPVSLLRRRPDVRAAERTLAADTAKIGQAEAARYPSLKLFGVIGLGGTHPSD 360 PPQQVAVGDPVSLLRRRPDVRAAERTLAADTAKIGQAEAARYPSLKLFGVIGLGGTHPSD Sbjct: 292 PPQQVAVGDPVSLLRRRPDVRAAERTLAADTAKIGQAEAARYPSLKLFGVIGLGGTHPSD 351 Query: 361 LTRLDDFAALGAPMLSWNFLDFGRAKARVTQAEQVRDEAEMKYRQSVLEALRDAEDALSR 420 LTRLDDFAALGAPMLSWNFLDFGRAKARVTQAE+VRDEAEMKYRQSVLEALRDAEDALSR Sbjct: 352 LTRLDDFAALGAPMLSWNFLDFGRAKARVTQAERVRDEAEMKYRQSVLEALRDAEDALSR 411 Query: 421 FRYGRVSVATIARSKVSADRALVLARQRYQAGTGTLIEVLDTTRQQITAQQNLLQAEANL 480 FRYGRVSVATIAR+K+SADRAL LARQRYQAGTGTLIEVLDTTRQQITAQQNLLQAEANL Sbjct: 412 FRYGRVSVATIARTKLSADRALALARQRYQAGTGTLIEVLDTTRQQITAQQNLLQAEANL 471 Query: 481 TRSFVGIQKALGLGWSEE 498 TRSFVGIQKALGLGWS+E Sbjct: 472 TRSFVGIQKALGLGWSDE 489 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 176 bits (445), Expect = 5e-45, Method: Compositional matrix adjust. Identities = 160/488 (32%), Positives = 246/488 (50%), Gaps = 49/488 (10%) Query: 32 LLAGCSALGPDYAGPPEAAPQAAHSQQFVRGGDSAN----SAAPINQ---WWQGLGDSEL 84 LL GC+ L PDY P AP AAH G DS N S+AP+ W D L Sbjct: 23 LLGGCT-LMPDYHRPD--APVAAH----FAGADSKNAAGASSAPVVADIGWRDVFTDPSL 75 Query: 85 NSLIERALQANPNLGVARARLQQSRAGLALEQANAAPSVGASALAGHARLPPANLGALTG 144 +I AL+ N +L VA ++++RA +++A PS+ A+ +R P Sbjct: 76 QQVIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAATSETASRTP--------- 126 Query: 145 GSSSGNGSPTSANLYS--VGFDASWEIDLFGARR----RAVEAATATADAAQASLADVQV 198 G S GS ++ Y+ +GF A +E+DLFG R +A+++ +TA+A +++ Q+ Sbjct: 127 GDLSSTGSALTSRSYTATLGFSA-YELDLFGRVRSLKEQALQSFLSTAEARRST----QI 181 Query: 199 SLAAEVANAYISLRDRQLRLSLGQEAVQAQEQMLQLTGQRVERGTASSLDQLRVQNQLDA 258 SL AEVA A+++L Q RL L Q+ +++Q LT + E G++S+L + Q +++ Sbjct: 182 SLIAEVATAWLTLASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQTSVES 241 Query: 259 SRADLVPLQAERDAFLNQLATLLGQE-------PGTLDSELAATAPV-PLPPQQVAVGDP 310 +R D+ A+ D N L L+G++ G D+ LAA +P+ +PP G P Sbjct: 242 ARVDVESYTAQVDQDRNALVLLVGKDLPTALLPQGLPDTRLAAASPLATIPP-----GLP 296 Query: 311 VSLLRRRPDVRAAERTLAADTAKIGQAEAARYPSLKLFGVIGLGGTHPSDLTRLDDFAAL 370 LL+RR D+ AER L A A IG A AA YPS+ L G + L + + Sbjct: 297 SDLLQRRADIVQAERDLRAANANIGAARAAFYPSITLTVNAGTASARLAGLFKGGSGSWS 356 Query: 371 GAPMLSWNFLDFGRAKARVTQAEQVRDEAEMKYRQSVLEALRDAEDALS-RFRYGRVSVA 429 P +S D G +A + A RD + +Y +++ A R+ DAL+ R GR A Sbjct: 357 FVPQISLPIFDGGANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQA 416 Query: 430 TIARSKVSADRALVLARQRYQAGTGTLIEVLDTTRQQITAQQNLLQAEANLTRSFVGIQK 489 A + S D A L++ RY +G + + VLD+ R +AQ+NL+ + + + V K Sbjct: 417 QEALVQAS-DEAFRLSQARYHSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLVTFYK 475 Query: 490 ALGLGWSE 497 LG GW E Sbjct: 476 TLGGGWVE 483 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 165 bits (417), Expect = 9e-42, Method: Compositional matrix adjust. Identities = 157/480 (32%), Positives = 235/480 (48%), Gaps = 47/480 (9%) Query: 35 GCSALGPDYAGPPEAAPQAAHSQQFVRGGDSANSAAPINQWWQGLGDSELNSLIERALQA 94 GC++ GPDY P P A S+ R A+ A P WW+ GD++LN+L ++AL Sbjct: 18 GCAS-GPDYTRPQVDMPAAWTSEAPWRSMQPAD-ATPRGPWWERFGDAQLNALQQQALAG 75 Query: 95 NPNLGVARARLQQSRAGLALEQANAAPSVGASALAGHARL----PPANLGALTGGSSSGN 150 N L +A ARL Q+RA L + A P V +A A +R+ P N +S N Sbjct: 76 NQTLAIATARLAQARAQLNVTGAAQTPQVNLNARAARSRISANRPLTNY-------NSPN 128 Query: 151 GSPTSANLYSVGFDASWEIDLFGARRRAVEAATATADAAQASLADVQVSLAAEVANAYIS 210 S T N +++G S+E+D FG +R+VEAA A A+ + A L + ++ L AE+A Y + Sbjct: 129 FS-TVQNDFALGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAELATNYFN 187 Query: 211 LRDRQLRLSLGQEAVQAQEQMLQLTGQRVERGTASSLDQLRVQNQLDASRADLVPLQAER 270 LR+ + L + + A+ Q Q L+L R + G S LD + Q LD++ + L +R Sbjct: 188 LRELDVELDVVRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQIDLLGRQR 247 Query: 271 DAFLNQLATLLGQEPGTLDSELAATAPVPLPPQQVAVGDPVSLLRRRPDVRAAERTLAAD 330 + + +ATL G + T P+ P + VG P +L RRPD+ AAER +AA Sbjct: 248 AQYEHAIATLTGTPAPAFSITPSLT---PITPPAIPVGVPSDVLERRPDIAAAERAMAAA 304 Query: 331 TAKIGQAEAARYPSLKLFGVIGLGGTHPSDLTRLDDFAALGAPMLSW--------NFLDF 382 A+IG A AA YPS L G+ + + L AP L W + D Sbjct: 305 NAQIGVASAAYYPSFMLQPSYGVDSRNWATL--------FNAPSLLWSLGVSASQSLFDG 356 Query: 383 GRAKARVTQAEQVRDEAEMKYRQSVLEALRDAEDALSRFRYGRVSVATIARSKVSADRAL 442 GR +A V ++ + YR++VL A+++ ED ++ + A + SA R L Sbjct: 357 GRLRAGVDFSQAGYEATVASYRRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVL 416 Query: 443 VLARQRYQAGTGTLIEVLDTTRQQITAQQNLLQAEANLTRSF-------VGIQKALGLGW 495 +A RY+ G ++V ITAQQ+LL +E + V + KALG W Sbjct: 417 EIASSRYEGGATPYLDV-------ITAQQSLLNSERQAAQLMGQRLLVSVFLIKALGGDW 469 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 160 bits (404), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 151/476 (31%), Positives = 228/476 (47%), Gaps = 27/476 (5%) Query: 35 GCSALGPDYAGP----PEAAPQAAHSQQFVRGGDSANSAAPIN-----QWWQGLGDSELN 85 GCS + P Y P P A +AA + Q G +A P +WW+ GD +LN Sbjct: 30 GCS-VAPTYERPSVDTPAAFKEAAPATQTGENGSQWKTAQPAEDIARGEWWKIFGDEKLN 88 Query: 86 SLIERALQANPNLGVARARLQQSRAGLALEQANAAPSVGASALAGHARLPPANLGALTGG 145 +L + A QAN +L A ARL Q+RA +A P V A A R PA+ G Sbjct: 89 ALEDTAQQANQDLKAAAARLGQARALTRDARAGYFPQVDAGVGATRQRPSPASQGLPADA 148 Query: 146 SSSGNGSPTSANLYSVGFDASWEIDLFGARRRAVEAATATADAAQASLADVQVSLAAEVA 205 + N LY + S+E+DLFG V+AATA A ++A V ++L A+VA Sbjct: 149 DTRPN------TLYRAQVNVSYEVDLFGRVASTVDAATADAQRSEALFHSVLLALQADVA 202 Query: 206 NAYISLRDRQLRLSLGQEAVQAQEQMLQLTGQRVERGTASSLDQLRVQNQLDASRADLVP 265 Y +R+ +L + V +++ L+L +R + G S +D R + +L +++++ + Sbjct: 203 QQYFLVRELDAAAALYRGTVDLRQESLRLVQRRFDEGDISEVDVARARAELASAQSEALG 262 Query: 266 LQAERDAFLNQLATLLGQEPGTLDSELAATAPVPLPPQQVAV--GDPVSLLRRRPDVRAA 323 + +R + LA LLG+ P + P PL V V G P +LL RRPD+ AA Sbjct: 263 IARQRATAEHALAILLGRTPAEF-----SLPPQPLTRLAVTVPPGLPSALLERRPDIAAA 317 Query: 324 ERTLAADTAKIGQAEAARYPSLKLFGVIGLGGTHPSDLTRLDDFAALGAPM----LSWNF 379 ER +AA A++G A++A +PSL L G +G DL L P+ LS Sbjct: 318 ERAMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSRTFLLGPLVGTALSMPL 377 Query: 380 LDFGRAKARVTQAEQVRDEAEMKYRQSVLEALRDAEDALSRFRYGRVSVATIARSKVSAD 439 D GR +A V +A +E YR +VL A R+ ED L+ R + ++ Sbjct: 378 FDGGRRQAGVDRARASYEEQVASYRGTVLNAFREVEDNLANLRILDQQNRAQDEAVAASA 437 Query: 440 RALVLARQRYQAGTGTLIEVLDTTRQQITAQQNLLQAEANLTRSFVGIQKALGLGW 495 RA L+ +Y+ G + V+D R + Q+ + + RS V + +ALG GW Sbjct: 438 RAAKLSHTQYREGAVSYFSVIDADRTVLQQQRASVTLDGERARSTVNLIRALGGGW 493 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 155 bits (393), Expect = 6e-39, Method: Compositional matrix adjust. Identities = 150/511 (29%), Positives = 232/511 (45%), Gaps = 67/511 (13%) Query: 22 AALKKERRPALLAGCSAL------GPDYAGPPEAAPQAAHSQQFVRGGDSANSAA--PIN 73 A L R P LL C+AL G P PQA + D+A A +N Sbjct: 2 ANLSMPRSPRLLHMCAALVMAGVLGGCSLAPTYERPQAPVESSY--PADAAGRAELQAVN 59 Query: 74 QWW-QGLGDSELNSLIERALQANPNLGVARARLQQSRAGLALEQANAAPSVGASALAGHA 132 W Q D L LI AL+ N +L A ++++RA + +A+ P++ S A Sbjct: 60 LGWRQFFPDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAART 119 Query: 133 RLPPANLGALTGGSSSGNGSPTSANLYSVGFDA-SWEIDLFGARRRAVEAATATADAAQA 191 R T S S G PT +N Y VG ++E+D FG R +AA A+ + + Sbjct: 120 R---------TAASQSLTGQPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEE 170 Query: 192 SLADVQVSLAAEVANAYISLRDRQLRLSLGQEAVQAQEQMLQLTGQRVERGTASSLDQLR 251 + Q+SL A+VA AY++ R +L L E +++++ L L +R++ G +++LD Sbjct: 171 AQKSAQISLVAQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRL 230 Query: 252 VQNQLDASRADLVPLQAERDAFLNQLATLLGQEPGTLDSELAATAPVPLPPQQ------- 304 Q ++++R L+ +R N L L+G++ G L PP Q Sbjct: 231 TQTLVESARVAKTQLERQRAQAENALTLLVGKKIGEL------------PPAQLLSDEKI 278 Query: 305 ---VAVGDPVSLLRRRPDVRAAERTLAADTAKIGQAEAARYPSLKLFGVIGLGGTHPSDL 361 + G P LL RPD+R+AE++L A A IG A AA +P + L G G + L Sbjct: 279 VTDIPAGLPSDLLTNRPDIRSAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGL 338 Query: 362 TRLDDFAALGAPMLSWNFLDFGRAKARVTQAEQVRDEAEMKYRQSVLEALRDAEDALSRF 421 A P LS DFGR A + AE +++A + Y +++ A R+ DAL Sbjct: 339 FESGSGAWSFGPSLSLPIFDFGRNSANLDLAEVRKNKAVVTYEKAIQTAFREVADAL--- 395 Query: 422 RYGRVSVATIAR----SKVSADRALVLARQ--------RYQAGTGTLIEVLDTTRQQITA 469 +AR +V A A + A+Q R++ G + ++ LD R +A Sbjct: 396 ---------VARGSLDEQVKAQEAFLAAQQERLKLTDLRFKNGIASSLDQLDAQRDLFSA 446 Query: 470 QQNLLQAEANLTRSFVGIQKALGLGWSEEAL 500 QQ L+QA + + + KALG G +E + Sbjct: 447 QQALIQARQLRLNNAIDLYKALGGGLNENTV 477 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.128 0.354 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,246,421 Number of extensions: 46598 Number of successful extensions: 322 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 257 Number of HSP's successfully gapped: 18 Length of query: 500 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 411 Effective length of database: 1,198,455 Effective search space: 492565005 Effective search space used: 492565005 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)