BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0521.p (522 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312486|ref|YP_003776578.1| major facilitator superfamily p... 910 0.0 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 218 7e-58 gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 216 5e-57 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 118 1e-27 gi|300311185|ref|YP_003775277.1| major facilitator superfamily p... 109 4e-25 >gi|300312486|ref|YP_003776578.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 514 Score = 910 bits (2352), Expect = 0.0, Method: Compositional matrix adjust. Identities = 499/510 (97%), Positives = 504/510 (98%) Query: 13 GAQPEKADAAAWLAVAAGTLGALMATLDISIVNSSLPTIQGEIGASGTEGTWIATAYLVA 72 GA EKADAAAWLAVAAGTLGALMATLDISIVNSSLPTIQGEIGASGTEGTWIATAYLVA Sbjct: 5 GANGEKADAAAWLAVAAGTLGALMATLDISIVNSSLPTIQGEIGASGTEGTWIATAYLVA 64 Query: 73 EIVIIPMSAWLERLLGLRVLLLTAASLFTLFSVLCGLSTTLPMMIVGRVGQGFTGGALIP 132 EIVIIPMSAWLERLLGLRVLLLTAASLFTLFSVLCGLSTTLPMMIVGRVGQGFTGGALIP Sbjct: 65 EIVIIPMSAWLERLLGLRVLLLTAASLFTLFSVLCGLSTTLPMMIVGRVGQGFTGGALIP 124 Query: 133 TAMTIIATRLPRSQQPVGNALFGATAILGPVLGPVMGGWLTENVSWHYAFFINLPVGAAL 192 TAMTIIATRLPRSQQPVGNA+FGATAILGPVLGPVMGGWLTENVSWHYAFFINLPVGAAL Sbjct: 125 TAMTIIATRLPRSQQPVGNAMFGATAILGPVLGPVMGGWLTENVSWHYAFFINLPVGAAL 184 Query: 193 ITLLLVAIPYERPKFEQLAEADWFGIAGLALGLGGLTVVLEEGEREQWFSSPDIRWLAAT 252 ITLLLVAIPYERPKFEQLAEADWFGIAGLALGLGGLTVVLEEGEREQWFSSPDIRWLAAT Sbjct: 185 ITLLLVAIPYERPKFEQLAEADWFGIAGLALGLGGLTVVLEEGEREQWFSSPDIRWLAAT 244 Query: 253 SVLGFILLAIGQVRARHPVIQLRLLRDRQFGSVVMMAMIVGVALYGTAYVIPQFLSGIAG 312 SVLGFILLAIGQVRARHPVIQLRLLRDRQFGSVVMMAMIVGVALYGTAYVIPQFLSGIAG Sbjct: 245 SVLGFILLAIGQVRARHPVIQLRLLRDRQFGSVVMMAMIVGVALYGTAYVIPQFLSGIAG 304 Query: 313 YNSLQSGWIVLLSGVPMILMMPFTPLLMRLVDIRIAVGTGFLLLATSAFIETDLSPLSYG 372 YNSLQSGWIVLLSGVPMILMMPFTPLLMRLVDIRIAVGTGFLLLA SAFIETDLSPLSYG Sbjct: 305 YNSLQSGWIVLLSGVPMILMMPFTPLLMRLVDIRIAVGTGFLLLAASAFIETDLSPLSYG 364 Query: 373 GSFVASQLMRGVGTVLAMLFLNQAAIRSVPPSQAGDASGLYSAARNLGGSLALAAIAVIQ 432 GSFVASQLMRGVGTVLAMLFLNQAAIRSVPPSQAGDASGLYSAARNLGGSLALAAIAVIQ Sbjct: 365 GSFVASQLMRGVGTVLAMLFLNQAAIRSVPPSQAGDASGLYSAARNLGGSLALAAIAVIQ 424 Query: 433 DQRLWLHSRRLEESLNANSDLVQSGIAAQAHLLGGQDVAIQLMGATIQAQALTMTYADLF 492 DQRLWLHSRRLEESLNANSDLVQS + AQ HLLGGQDVA+QLMGATIQ+QALTMTYADLF Sbjct: 425 DQRLWLHSRRLEESLNANSDLVQSSMTAQGHLLGGQDVALQLMGATIQSQALTMTYADLF 484 Query: 493 WMLGVAILCVTPLVLFLRPLPTNAAPVASH 522 WMLGVAILCVTPLVLFLRPLPT+AAPVASH Sbjct: 485 WMLGVAILCVTPLVLFLRPLPTHAAPVASH 514 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 218 bits (556), Expect = 7e-58, Method: Compositional matrix adjust. Identities = 142/471 (30%), Positives = 240/471 (50%), Gaps = 8/471 (1%) Query: 31 TLGALMATLDISIVNSSLPTIQGEIGASGTEGTWIATAYLVAEIVIIPMSAWLERLLGLR 90 +L M LD SI N ++PTI G +G S EGTW+ T++ A + IP++ WL + LG Sbjct: 30 SLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGAV 89 Query: 91 VLLLTAASLFTLFSVLCGLSTTLPMMIVGRVGQGFTGGALIPTAMTIIATRLPRSQQPVG 150 L +T+ LF L S LCGL+ TLP+++ RV QG G ++P + +++ P+S+ Sbjct: 90 RLFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKSSFA 149 Query: 151 NALFGATAILGPVLGPVMGGWLTENVSWHYAFFINLPVG--AALITLLLVAIPYERPKFE 208 AL+G TA++ PV GP +GGW+T++ +W + F+IN+PVG AA +TL AI R Sbjct: 150 LALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTL---AIYRSRETPT 206 Query: 209 QLAEADWFGIAGLALGLGGLTVVLEEGEREQWFSSPDIRWLAATSVLGFILLAIGQVRAR 268 D G+A L + ++L++G+ WF+SP I L +++ F + ++ Sbjct: 207 HKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGIIALIAFAYFVVWELNND 266 Query: 269 HPVIQLRLLRDRQF-GSVVMMAMIVGVALYGTAYVIPQFLSGIAGYNSLQSGWIVLLSGV 327 HPV+ LRL R F G + +++ GV +G ++PQ+L GY S+ SG G+ Sbjct: 267 HPVVDLRLFGRRNFAGGTIAISIGYGV-FFGNLVILPQWLQQYLGYRSIDSGLSTAPIGI 325 Query: 328 PMILMMPFTPLLMRLVDIRIAVGTGFLLLATSAFIETDLSPLSYGGSFVASQLMRGVGTV 387 +++MP + LVD R F+ A ++ + + + + L++G+ T Sbjct: 326 FAVILMPLIGRFLPLVDARKVATASFIGFAIVFWLRSRYNLQVDQMTVILPTLLQGIPTA 385 Query: 388 LAMLFLNQAAIRSVPPSQAGDASGLYSAARNLGGSLALAAIAVIQDQRLWLHSRRLEESL 447 + + L + +PP + A+GL S R G++ + + R +H +L E Sbjct: 386 MFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTAWNNRSIMHHAQLTEHA 445 Query: 448 NANSDLVQSGIAAQAHLLG-GQDVAIQLMGATIQAQALTMTYADLFWMLGV 497 S +Q + + LG + ++ TI QA + D+F++ V Sbjct: 446 TPYSPWLQETVNNLSSTLGLNAEQTAGMLERTITFQAAMLGINDIFYVSAV 496 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 216 bits (549), Expect = 5e-57, Method: Compositional matrix adjust. Identities = 150/498 (30%), Positives = 259/498 (52%), Gaps = 25/498 (5%) Query: 35 LMATLDISIVNSSLPTIQGEIGASGTEGTWIATAYLVAEIVIIPMSAWLERLLG-LRVLL 93 +A LD +I N S+ TI G +GAS ++GT++ T+Y VAE + +P++ WL G +RV + Sbjct: 43 FIAVLDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFI 102 Query: 94 LTAASLFTLFSVLCGLSTTLPMMIVGRVGQGFTGGALIPTAMTIIATRLPRSQQPVGNAL 153 L+ +F LFS LCG++ +L M+I RV QG GG L+P + T++ P+ + P L Sbjct: 103 LSMV-MFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAPAAVGL 161 Query: 154 FGATAILGPVLGPVMGGWLTENVSWHYAFFINLPVGAALITLLLVAIPYERPKFEQLAEA 213 + T ++ P+ GP++GG L + SW Y F+IN+PV + + K +L Sbjct: 162 WAMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYIGWRMLKRYETKMSKL-PI 220 Query: 214 DWFGIAGLALGLGGLTVVLEEGEREQWFSSPDIRWLAATSVLGFILLAIGQVRARHPVIQ 273 D G+ + + + L ++L+EG+ + WF+S +I LA T+V+ FI I ++ R+P++ Sbjct: 221 DRVGLVLMVVWVAALQLMLDEGKEKDWFASTEIVLLAVTAVVFFIAFLIWELTERNPIVD 280 Query: 274 LRLLRDRQFGSVVMMAMIVGVALYGTAYVIPQFLSGIAGYNSLQSGWIVLLSGVPMILMM 333 LR+ R R F + V + A +G+ + P +L G GY + SG I L+G+ ++ Sbjct: 281 LRVFRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWSGCISALTGILAVMTA 340 Query: 334 PFTPLLMRLVDIR--IAVGTGFLLLAT--SAFIETDLSPLSYGGSFVASQLMRGVGTVLA 389 P + +D R + +G +L T AF TD+S G ++G+G L Sbjct: 341 PMVAKMSTTIDARKLVCIGVTWLGFITFLRAFNTTDMSFFQIGWPL----FLQGIGLPLF 396 Query: 390 MLFLNQAAIRSVPPSQAGDASGLYSAARNLGGSLALAAIAVI-QDQRLWLH---SRRLEE 445 + L A+ SV + A+GL S R + G++A + + ++ + H S ++ Sbjct: 397 FVPLTGLALSSVNEEETASAAGLMSFCRTMAGAIATSIVNTSWENDATYFHAELSGLVDR 456 Query: 446 SLNANSDLVQSGIAAQAHLLGGQDVAIQLMGATIQAQALTMTYADLFWMLGVAILCVTPL 505 +A ++ SG++A+ A + T+Q QA+ ++ +F M V+ + L Sbjct: 457 GGDAIHTMMASGMSAEQ--------ATASLAQTVQNQAVMLSTNHIFMMAAVS-FSLAAL 507 Query: 506 VLFLRPLPTNAAPV-ASH 522 ++L P PT A A+H Sbjct: 508 AVWLAPKPTRVADTSAAH 525 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 118 bits (295), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 109/397 (27%), Positives = 184/397 (46%), Gaps = 24/397 (6%) Query: 33 GALMATLDISIVNSSLPTIQGEIGASGTEGTWIATAYLVAEIVIIPMSAWLERLLGLRVL 92 G M TLD +IVN++LP + +G + AY + + P S WL G R + Sbjct: 61 GFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFGTRRM 120 Query: 93 LLTAASLFTLFSVLCGLSTTLPMMIVGRVGQGFTGGALIPTAMTIIATRLPRSQQPVGNA 152 A LF+L S+LC ++++LPM++ RV QG G L+P + +P +Q A Sbjct: 121 YFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYIAALA 180 Query: 153 LFGATAILGPVLGPVMGGWLTENVSWHYAFFINLPVGAALITLLLVAIPYER----PKFE 208 LGPV+GPV+GGWL E++SWH+ F INLPVG + +L +P + P+F Sbjct: 181 SISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLAGTPRF- 239 Query: 209 QLAEADWFGIAGLALGLGGLTVVLE---EGEREQWFSSPDIRWLAATSVLGFILLAIGQV 265 D G L+ + ++ L+ + R W S + +AA +VLG+ A Sbjct: 240 -----DLLGCVLLSACMVAFSLALDVPFDSHRTGW--SIALFVVAALTVLGYWRHASVHP 292 Query: 266 RARHPVIQLRLLRDRQFGSVVMMAMIVGVALYGTAYVIPQFLSGIAGYNSLQSGWIVLLS 325 R P+ +L L + F + ++ + +++P L GY+ L +G ++L + Sbjct: 293 R---PLFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLPA 349 Query: 326 GVPMILMMPF-TPLLMRL-VDIRIAVGTGFLLLATSAF-IETDLSPLSYGGSFVASQLMR 382 + + P+ PL+ R D + V T + A +F + T PL+ + + Sbjct: 350 AIAGTIAKPWIAPLVKRFGYDRFLLVNTAIVGAAIMSFALITPDWPLTAA---IVQLAIF 406 Query: 383 GVGTVLAMLFLNQAAIRSVPPSQAGDASGLYSAARNL 419 G + +N ++ + AG + L+S + L Sbjct: 407 GASNSMQFAAMNSVTLKGLDKRDAGSGNSLFSMVQML 443 >gi|300311185|ref|YP_003775277.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 532 Score = 109 bits (273), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 117/464 (25%), Positives = 213/464 (45%), Gaps = 37/464 (7%) Query: 35 LMATLDISIVNSSLPTIQGEIGASGTE-GTWIATAYLVAEIVIIPMSAWLERLLGLRVLL 93 ++ LD +IV ++LPTI E+ G E W+ATAYL+ ++ +P+ L G + + Sbjct: 32 MLVALDQTIVGTALPTIVAEL--KGFELYAWVATAYLLTSVITVPIFGRLGDYFGRKPFV 89 Query: 94 LTAASLFTLFSVLCGLSTTLPMMIVGRVGQGFTGGALIPTAMTIIATRLPRSQ-----QP 148 + + +F S LCG++ ++ +++ R QG GG L+ T +A P + Q Sbjct: 90 IASIIVFVGASALCGMANSMLFLVLARGLQGIGGGMLVGTCFASVADLFPDPRVRLRWQI 149 Query: 149 VGNALFG-ATAILGPVLGPVMGGWLTENVSWHYAFFINLPVGAALITLLLVAIPYERP-K 206 + + FG ATA+ GP +GG+LT+ + W + F+ NLP+G + + +P+ R + Sbjct: 150 ILSVSFGIATAV-----GPSLGGFLTQGLGWRWVFYCNLPIGVISLFFVWRFVPHIRHMQ 204 Query: 207 FEQLAEADWFGIAGLALGLGGLTVVLEEGEREQWFSSPDIRWLAATSVLGFILLAIGQVR 266 DW G ++L LG L +++E + S + L A SV F L + R Sbjct: 205 HAGKMRLDWPGALLISLSLGSLQLLVELLPKRGVTSGMLV--LLAVSVAAFYALWKWEQR 262 Query: 267 ARHPVIQLRLLRDRQFGSVVMMAMIVGVALYGTAYVIPQFLSGIAGYNSLQSGWIVLLSG 326 A P++ +LRDR S+ +++++ G A++ P G G + ++G ++ Sbjct: 263 AAQPILPFEMLRDRALASLFLLSVLAGFAMFSMLMYAPLLFQGGFGLSPREAGLLI---- 318 Query: 327 VPMILMMPFTP-----LLMRLVDIRIAVGTGFLLLATSAFIETDLSPLSYGGSFVASQLM 381 P++ + ++ R+ + + + GF L+A S ++ G +A L Sbjct: 319 TPLVACITVASIVNGRIITRIPNPNLMMNLGFALIAASCLGIVLCDRVTGKGFLLAVMLA 378 Query: 382 RGVGTVLAM----LFLNQAAIRSVPPSQAGDASGLYSAARNLGGSLALAAIAVIQDQRLW 437 G G L + +F QAA R G A+ L + R +GG L A + Q Sbjct: 379 GGFGLGLVLPNLTVFAQQAAAR----EHLGIATALLQSLRMIGGMLGTAITGTLVSQ--- 431 Query: 438 LHSRRLEESLNANSDLVQSGIAAQAHLLGGQDVAIQLMGATIQA 481 ++ +E++L + + +L D+ L+ +QA Sbjct: 432 MYGAGVEKALEGDHAAQWLKDFSDPEVLVNHDIQSVLLAQLMQA 475 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.138 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,544,028 Number of extensions: 63129 Number of successful extensions: 388 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 364 Number of HSP's successfully gapped: 15 Length of query: 522 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 432 Effective length of database: 1,193,720 Effective search space: 515687040 Effective search space used: 515687040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 133 (55.8 bits)