BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0520.p (225 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312487|ref|YP_003776579.1| multidrug resistance transport ... 396 e-112 gi|300312565|ref|YP_003776657.1| multidrug resistance transmembr... 136 1e-33 gi|300312016|ref|YP_003776108.1| multidrug resistance A (translo... 111 4e-26 gi|300311421|ref|YP_003775513.1| multidrug resistance efflux pum... 58 8e-10 >gi|300312487|ref|YP_003776579.1| multidrug resistance transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 397 Score = 396 bits (1018), Expect = e-112, Method: Compositional matrix adjust. Identities = 217/225 (96%), Positives = 223/225 (99%) Query: 1 LAASGAETRERLTTLRNQQAQARSTVAARRAALDAAQLRVRSLQAQVRQAQAQGETAKAQ 60 LAASGAETRERLTTL NQQAQARSTVAARRAALDAAQLRVRSLQAQVRQAQAQGETAKAQ Sbjct: 173 LAASGAETRERLTTLHNQQAQARSTVAARRAALDAAQLRVRSLQAQVRQAQAQGETAKAQ 232 Query: 61 YDAASVNLGYTEIRAAVDGRIGDKQVRVGQFVSPGTRMMTVVPTRFYVSANFKETQIGIM 120 YDAASVNLGYTEIRA+VDGRIGDKQVRVGQFVSPGTRMMTVVPTRFYVSANFKETQIGIM Sbjct: 233 YDAASVNLGYTEIRASVDGRIGDKQVRVGQFVSPGTRMMTVVPTRFYVSANFKETQIGIM 292 Query: 121 RIGQPAIIKVDALPGVEFHGHVESLSPGTGAQFSLLPPQNATGNFTKIVQRLPVRVAVDA 180 RIGQPAIIKVDALPGVEFHGHVESLSPGTGAQFSLLPPQNATGNFTKIVQR+PVRVA+DA Sbjct: 293 RIGQPAIIKVDALPGVEFHGHVESLSPGTGAQFSLLPPQNATGNFTKIVQRVPVRVAIDA 352 Query: 181 TPEETRAFVAGLSVTVEVNTIAAKDELRELRERNSESNATRKESQ 225 TPEETRAFVAGLSVTVEVNTIAAKDELR+LRER+SE+NATRKE Q Sbjct: 353 TPEETRAFVAGLSVTVEVNTIAAKDELRDLRERSSEANATRKEPQ 397 >gi|300312565|ref|YP_003776657.1| multidrug resistance transmembrane efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 411 Score = 136 bits (343), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 70/146 (47%), Positives = 99/146 (67%), Gaps = 2/146 (1%) Query: 57 AKAQYDAASVNLGYTEIRAAVDGRIGDKQVRVGQFVSPGTRMMTVVPTR-FYVSANFKET 115 A+A+ + A ++L T +RA VDG I +QV+VGQ V G +++VVP + +V ANFKE Sbjct: 242 ARAKLEQAKLDLERTVLRAPVDGVIARRQVQVGQRVQSGATLLSVVPLQQMHVDANFKEG 301 Query: 116 QIGIMRIGQPAIIKVDALPG-VEFHGHVESLSPGTGAQFSLLPPQNATGNFTKIVQRLPV 174 Q+ +RIGQP +K D G VE+HG V LS GTG+ F+++P QNATGN+ K+VQRLPV Sbjct: 302 QLTKVRIGQPVTMKADLYGGSVEYHGVVTGLSGGTGSAFAVIPAQNATGNWIKVVQRLPV 361 Query: 175 RVAVDATPEETRAFVAGLSVTVEVNT 200 R+++D R GLS+ VE++T Sbjct: 362 RISLDPKELAQRPLSVGLSMVVEIDT 387 >gi|300312016|ref|YP_003776108.1| multidrug resistance A (translocase) transmembrane secretion protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 111 bits (278), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 62/140 (44%), Positives = 86/140 (61%), Gaps = 6/140 (4%) Query: 64 ASVNLGYTEIRAAVDGRIGDKQVRVGQFVSPGTRMMTVVPT-RFYVSANFKETQIGIMRI 122 A +N I A V G + + V+VGQ ++ G +M +VP + ++ ANFKE+Q+ +RI Sbjct: 209 AYLNYARVNIVAPVSGFVSKRSVQVGQRIAAGNPLMAIVPLEQIWIDANFKESQLQHIRI 268 Query: 123 GQPAIIKVDAL-PGVEFHGHVESLSPGTGAQFSLLPPQNATGNFTKIVQRLPVRVAVDAT 181 GQP + D V++ G V S GTG FSLLP QNATGN+ K+VQR+PVR+A+D Sbjct: 269 GQPVEVIADVYGSSVKYKGTVIGFSAGTGGAFSLLPAQNATGNWIKVVQRVPVRIALD-- 326 Query: 182 PEETRA--FVAGLSVTVEVN 199 PE+ RA GLS T V+ Sbjct: 327 PEQVRAHPLRIGLSTTATVD 346 >gi|300311421|ref|YP_003775513.1| multidrug resistance efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 324 Score = 57.8 bits (138), Expect = 8e-10, Method: Compositional matrix adjust. Identities = 65/209 (31%), Positives = 108/209 (51%), Gaps = 21/209 (10%) Query: 6 AETRERLTTLR------NQQAQARSTVAARRAALDAAQ------LRVRSLQAQVRQAQAQ 53 AE R L ++A + V +R A DA ++ + QAQ++ AQAQ Sbjct: 104 AEVEARRADLNMRRAQARRRADVDALVVSREAREDAGSQVTASQAQLEAAQAQLQGAQAQ 163 Query: 54 GETAKAQYDAASVNLGYTEIRAAVDGRIGDKQVRVGQFVSPGTRMMTVVPTR-FYVSANF 112 + A +Q D A +NL T++ + VDG I + VR G + + G + V+ + F+V A F Sbjct: 164 YQAALSQRDTARLNLERTKVFSPVDGYITNLNVRRGDYAAAGAAKIAVIDSHSFWVYAYF 223 Query: 113 KETQIGIMRIGQPAIIKVDALPGVEFHGHVESLSPGTGAQFS-----LLPPQNATGNFTK 167 +ET++ ++ IG A I++ + G GHV+S++ G + + L N T N+ + Sbjct: 224 EETKLPLLSIGDRARIQL--INGASMEGHVDSIARGIYDRDNPQSRELTADVNPTFNWVR 281 Query: 168 IVQRLPVRVAVDATPEETRAFVAGLSVTV 196 + QR+PVR+ +D PE +GL+ TV Sbjct: 282 LAQRIPVRIQLDKVPENV-LLASGLTCTV 309 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.127 0.339 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 555,034 Number of extensions: 20479 Number of successful extensions: 114 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 107 Number of HSP's successfully gapped: 5 Length of query: 225 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 143 Effective length of database: 1,231,600 Effective search space: 176118800 Effective search space used: 176118800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)